LOCUS       NM_016823               3850 bp    mRNA    linear   PRI 13-MAY-2024
DEFINITION  Homo sapiens CRK proto-oncogene, adaptor protein (CRK), transcript
            variant II, mRNA.
ACCESSION   NM_016823
VERSION     NM_016823.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Guo,C., Lv,X., Zhang,Q., Yi,L., Ren,Y., Li,Z., Yan,J., Zheng,S.,
            Sun,M.Z. and Liu,S.
  TITLE     CRKL but not CRKII contributes to hemin-induced erythroid
            differentiation of CML
  JOURNAL   J Cell Mol Med 28 (9), e18308 (2024)
   PUBMED   38683131
  REMARK    GeneRIF: CRKL but not CRKII contributes to hemin-induced erythroid
            differentiation of CML.
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Yin,Y., Zhang,L., Li,Y., Zhang,C. and He,A.
  TITLE     Gab2 plays a carcinogenic role in ovarian cancer by regulating
            CrkII
  JOURNAL   J Ovarian Res 16 (1), 79 (2023)
   PUBMED   37085900
  REMARK    GeneRIF: Gab2 plays a carcinogenic role in ovarian cancer by
            regulating CrkII.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3850)
  AUTHORS   Rodriguez-Blazquez,A., Carabias,A., Moran-Vaquero,A., de Cima,S.,
            Luque-Ortega,J.R., Alfonso,C., Schuck,P., Manso,J.A.,
            Macedo-Ribeiro,S., Guerrero,C. and de Pereda,J.M.
  TITLE     Crk proteins activate the Rap1 guanine nucleotide exchange factor
            C3G by segregated adaptor-dependent and -independent mechanisms
  JOURNAL   Cell Commun Signal 21 (1), 30 (2023)
   PUBMED   36737758
  REMARK    GeneRIF: Crk proteins activate the Rap1 guanine nucleotide exchange
            factor C3G by segregated adaptor-dependent and -independent
            mechanisms.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3850)
  AUTHORS   Benz,C., Ali,M., Krystkowiak,I., Simonetti,L., Sayadi,A.,
            Mihalic,F., Kliche,J., Andersson,E., Jemth,P., Davey,N.E. and
            Ivarsson,Y.
  TITLE     Proteome-scale mapping of binding sites in the unstructured regions
            of the human proteome
  JOURNAL   Mol Syst Biol 18 (1), e10584 (2022)
   PUBMED   35044719
REFERENCE   5  (bases 1 to 3850)
  AUTHORS   Hempstead,B.L., Birge,R.B., Fajardo,J.E., Glassman,R., Mahadeo,D.,
            Kraemer,R. and Hanafusa,H.
  TITLE     Expression of the v-crk oncogene product in PC12 cells results in
            rapid differentiation by both nerve growth factor- and epidermal
            growth factor-dependent pathways
  JOURNAL   Mol Cell Biol 14 (3), 1964-1971 (1994)
   PUBMED   7509449
REFERENCE   6  (bases 1 to 3850)
  AUTHORS   Schaller,M.D., Hildebrand,J.D., Shannon,J.D., Fox,J.W., Vines,R.R.
            and Parsons,J.T.
  TITLE     Autophosphorylation of the focal adhesion kinase, pp125FAK, directs
            SH2-dependent binding of pp60src
  JOURNAL   Mol Cell Biol 14 (3), 1680-1688 (1994)
   PUBMED   7509446
REFERENCE   7  (bases 1 to 3850)
  AUTHORS   Fioretos,T., Heisterkamp,N., Groffen,J., Benjes,S. and Morris,C.
  TITLE     CRK proto-oncogene maps to human chromosome band 17p13
  JOURNAL   Oncogene 8 (10), 2853-2855 (1993)
   PUBMED   8378094
REFERENCE   8  (bases 1 to 3850)
  AUTHORS   Cicchetti,P., Mayer,B.J., Thiel,G. and Baltimore,D.
  TITLE     Identification of a protein that binds to the SH3 region of Abl and
            is similar to Bcr and GAP-rho
  JOURNAL   Science 257 (5071), 803-806 (1992)
   PUBMED   1379745
REFERENCE   9  (bases 1 to 3850)
  AUTHORS   Matsuda,M., Tanaka,S., Nagata,S., Kojima,A., Kurata,T. and
            Shibuya,M.
  TITLE     Two species of human CRK cDNA encode proteins with distinct
            biological activities
  JOURNAL   Mol Cell Biol 12 (8), 3482-3489 (1992)
   PUBMED   1630456
REFERENCE   10 (bases 1 to 3850)
  AUTHORS   Mayer,B.J. and Hanafusa,H.
  TITLE     Association of the v-crk oncogene product with
            phosphotyrosine-containing proteins and protein kinase activity
  JOURNAL   Proc Natl Acad Sci U S A 87 (7), 2638-2642 (1990)
   PUBMED   1690891
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AU125411.1, BC008506.1 and
            AC032044.28.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_016823.3.
            
            Summary: This gene encodes a member of an adapter protein family
            that binds to several tyrosine-phosphorylated proteins. The product
            of this gene has several SH2 and SH3 domains (src-homology domains)
            and is involved in several signaling pathways, recruiting
            cytoplasmic proteins in the vicinity of tyrosine kinase through
            SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this
            protein functions as a positive regulator of transformation whereas
            the C-terminal SH3 domain functions as a negative regulator of
            transformation. Two alternative transcripts encoding different
            isoforms with distinct biological activity have been described.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (II) includes an alternate
            segment, compared to variant I, resulting in a longer protein
            (isoform a) that has a distinct C-terminus and an additional SH3
            domain, compared to isoform b. Isoform a is a negative modulator of
            transformation activity.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.154102.1,
                                           SRR1660807.101919.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000300574.3/ ENSP00000300574.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-164               AU125411.1         36-199
            165-2375            BC008506.1         183-2393
            2376-3850           AC032044.28        27165-28639
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p13.3"
     gene            1..3850
                     /gene="CRK"
                     /gene_synonym="CRKII; p38"
                     /note="CRK proto-oncogene, adaptor protein"
                     /db_xref="GeneID:1398"
                     /db_xref="HGNC:HGNC:2362"
                     /db_xref="MIM:164762"
     exon            1..356
                     /gene="CRK"
                     /gene_synonym="CRKII; p38"
                     /inference="alignment:Splign:2.1.0"
     CDS             116..1030
                     /gene="CRK"
                     /gene_synonym="CRKII; p38"
                     /note="isoform a is encoded by transcript variant II;
                     adapter molecule crk; proto-oncogene c-Crk; v-crk sarcoma
                     virus CT10 oncogene-like protein; v-crk avian sarcoma
                     virus CT10 oncogene homolog"
                     /codon_start=1
                     /product="adapter molecule crk isoform a"
                     /protein_id="NP_058431.2"
                     /db_xref="CCDS:CCDS11002.1"
                     /db_xref="GeneID:1398"
                     /db_xref="HGNC:HGNC:2362"
                     /db_xref="MIM:164762"
                     /translation="MAGNFDSEERSSWYWGRLSRQEAVALLQGQRHGVFLVRDSSTSP
                     GDYVLSVSENSRVSHYIINSSGPRPPVPPSPAQPPPGVSPSRLRIGDQEFDSLPALLE
                     FYKIHYLDTTTLIEPVSRSRQGSGVILRQEEAEYVRALFDFNGNDEEDLPFKKGDILR
                     IRDKPEEQWWNAEDSEGKRGMIPVPYVEKYRPASASVSALIGGNQEGSHPQPLGGPEP
                     GPYAQPSVNTPLPNLQNGPIYARVIQKRVPNAYDKTALALEVGELVKVTKINVSGQWE
                     GECNGKRGHFPFTHVRLLDQQNPDEDFS"
     exon            357..892
                     /gene="CRK"
                     /gene_synonym="CRKII; p38"
                     /inference="alignment:Splign:2.1.0"
     exon            893..3850
                     /gene="CRK"
                     /gene_synonym="CRKII; p38"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3804..3809
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CRK"
                     /gene_synonym="CRKII; p38"
                     /note="hexamer: AATAAA"
     polyA_site      3821
                     /gene="CRK"
                     /gene_synonym="CRKII; p38"
                     /note="major polyA site"
     polyA_site      3850
                     /gene="CRK"
                     /gene_synonym="CRKII; p38"
                     /note="major polyA site"
ORIGIN      
        1 atttcgggcg ctgctgtgaa gctgaaaccg gagccggtcc gctgggcggc gggcgccggg
       61 ggccggaggg gcgcgcgcgg cggcggcacc ccagcgttta ggcgcggagg cagccatggc
      121 gggcaacttc gactcggagg agcggagtag ctggtactgg gggaggttga gtcggcagga
      181 ggcggtggcg ctgctgcagg gccagcggca cggggtgttc ctggtgcggg actcgagcac
      241 cagccccggg gactatgtgc tcagcgtctc agagaactcg cgcgtctccc actacatcat
      301 caacagcagc ggcccgcgcc cgccggtgcc accgtcgccc gcccagcctc cgcccggggt
      361 gagcccctcc agactccgaa taggagatca agagtttgat tcattgcctg ctttactgga
      421 attctacaaa atacactatt tggacactac aacgttgata gaaccagttt ccagatccag
      481 gcagggtagt ggagtgattc tcaggcagga ggaggcggag tatgtgcgag ccctctttga
      541 ctttaatggg aatgatgagg aagatcttcc ctttaagaaa ggagacatct tgagaatccg
      601 ggacaagcct gaagagcagt ggtggaatgc ggaggacagc gaaggcaaga gagggatgat
      661 tccagtccct tacgtcgaga agtatagacc tgcctccgcc tcagtatcgg ctctgattgg
      721 aggtaaccag gagggttccc acccacagcc actgggtggg ccggagcctg ggccctatgc
      781 ccaacccagc gtcaacactc cgctccctaa cctccagaat gggcccatat atgccagggt
      841 tatccagaag cgagtcccca atgcctacga caagacagcc ttggctttgg aggtcggtga
      901 gctggtaaag gttacgaaga ttaatgtgag tggtcagtgg gaaggggagt gtaatggcaa
      961 acgaggtcac ttcccattca cacatgtccg tctgctggat caacagaatc ccgatgagga
     1021 cttcagctga gtatagttca acagttttgc tgacagatgg gaacaatctt tttttttttt
     1081 ttccaactgc catctataca attttcttac agatgtcaaa agcagtctag tttatataag
     1141 cattctgtta cctgtgatat tttttagact gaactgctcc attcctagtc ttaattacca
     1201 tattcagggt acgaactgga gggcttgtgt gttagcttct gaattggcaa ttggaggcgg
     1261 tagtggtcgt gcctgtgtgt atcagaaggg ataggtatct tgcctccttt ctctcaggca
     1321 gtgcaaatca ccctgtggaa aaccgatgga caggaaggag tgttacacac tgcttaccct
     1381 gatttattca gtggttttgt tttcattctg gaaccatact atcaaatggc gacagactgt
     1441 tccgttccac ccccgtgaag taatcatgca ccgtgtgaat agtatcaagc aggattgctt
     1501 tcattgtatg gagcatgacc agcgtgtgac tcattctgac atttcagatc ctaagaattc
     1561 taagaacact actagaagca tttgttccct cctagtcaat gcttcatact ttttcttggg
     1621 attcttttag cccttgacat tcttgtcccc caaacctgta agtaggtgaa ttcctaagat
     1681 aagtgtgtat tttcattcca ggtgaaaagc aggatgtacc gagcacttta ttcagtgcat
     1741 agctttaagc cagtgttgga ttcactaagt ggacagccag tctcccagct ctctgccttc
     1801 cccaaaaggg tcgtagtagg tcacccttct acagcagcta actagagtcc taactaatgg
     1861 gatccagcag ggccatttct ccagagggcc agtatcctat taggagactc ttggaattct
     1921 taggttctac tcaagagtgg aaggaccaat cacctctgat attctgtgga aggttttggg
     1981 gtcaaattct gccctctgca ttctgtgcaa cttgtataaa agtcaagtta gtattacatg
     2041 aatttggggt agggttagtg ctttgaaaaa atgttgaacc ggctgggcgc ggtggctcac
     2101 gtctgtaatc ccagcacttt gggaggccga ggcgggtgga tcatgaggtc aggagttcga
     2161 gaccagcctg gccaacatag tgaaacccca tctctgctaa agatataaaa aattagcccg
     2221 gcgtggtggt gcacgcctgt aatcccagct actcgggagg ctgaggcagg agaattgctt
     2281 caacctggga ggtggaggct gcagtgagcc gagatcgcac cactgcgttc cagcctgagc
     2341 gacagggcaa gactcagtct caaaaaaaaa aaaaaggaaa aaaaaaagaa aaaaaaatgt
     2401 tgaaccaatt gtgaattact tatgtattat tcatttctca tggggagagt aatgctgttg
     2461 aagaacatta cattgtaaac tgccttcatt tttggctctt tgtttatgtt caggtttagt
     2521 ttacaaaccc atttaagtat ggaatgattt atatggggtc aggtgctcca caaaatagac
     2581 ctatgagacc aaaaatgacc taggctattt agacgacagc atgaaacttc cacgttagtt
     2641 ctcagtctat aaaggcactt accggtctct ggtgtggtat gaccaataga aacaccttat
     2701 agtttgcttt ggacctcatt ttggaaaaat aatctgcctt tctaattgtt ctgcataggt
     2761 taaaatgata aatttacatt ctttgaacct ataccagatt gtggtgtccg agtgaccggc
     2821 acactgtctg acacacagtc agtgtgcacg tatttgtctg agtgaatgag gagacctgag
     2881 aaaccggtga cgtggcacag ggaagccagc tggcccagga ttccgtacat ggccgcaagc
     2941 agactaacgc gttgacgcta atttaatgta ttttacctca cactaaggtc atgcttgata
     3001 aagacgttaa actcaacttg taaaatggta gcccagtgct atgcacagag tgggtgctca
     3061 ttagtgttga atgaacacat ttgtaatact acatgtaatt ccatctgact gctttgttaa
     3121 attttcagtt agaacgtaga tactgtaaag tccacacaca cattaaatct tgttttcctg
     3181 aaagtatggc atcaaaaata cttgtagaaa aaccttgtca caactgattt gaatgttcct
     3241 attttctttt cctttgactt tgatattggc ttgtaatgtc tcttttcatc atatgtaata
     3301 tcagtggaac aggcagcgct actcaagtcc taaggattcc tcagtgatca gtgatccagg
     3361 gccgttcatg aaccactggg ctggatttga ctgttgagtg tggcagttaa tgcccctcaa
     3421 gaaatcaaag gatgtcttat aagtgtcttc caaaaaaaag caaatgctga aatcctattg
     3481 gcaaagtaaa ctgaaattgg ctgctatatt ttatataatc atttctgcaa atcccatttt
     3541 ttgaatacta atatttgaca tggttaattc ttattaattt gttggaattg tttattgtta
     3601 ataatgcaaa tagataattt ttaattatcc acaagtaaca tttcactgtt aatggtttga
     3661 aataggtgat aagcaaacca atttgaaata aaatataaac atgtgccatt gtattataac
     3721 actatacact ttcttgacag ttaaatttaa aaaaaaattt tttttggtag catgtattgt
     3781 atatgtttat agtatatgta gtaaataaaa atatggccaa atgtttggct tggtgatctt
     3841 ttgcaaaaaa
//
