LOCUS       NM_031476               4583 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens cysteine rich secretory protein LCCL domain containing
            2 (CRISPLD2), mRNA.
ACCESSION   NM_031476
VERSION     NM_031476.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Rong,W., Rome,C.P., Dietrich,M.A. and Yao,S.
  TITLE     Decreased CRISPLD2 expression impairs osteogenic differentiation of
            human mesenchymal stem cells during in vitro expansion
  JOURNAL   J Cell Physiol 238 (6), 1368-1380 (2023)
   PUBMED   37021796
  REMARK    GeneRIF: Decreased CRISPLD2 expression impairs osteogenic
            differentiation of human mesenchymal stem cells during in vitro
            expansion.
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Lavictoire,S.J., Jomaa,D., Gont,A., Jardine,K., Cook,D.P. and
            Lorimer,I.A.J.
  TITLE     Identification of Rac guanine nucleotide exchange factors promoting
            Lgl1 phosphorylation in glioblastoma
  JOURNAL   J Biol Chem 297 (5), 101172 (2021)
   PUBMED   34624316
  REMARK    GeneRIF: Identification of Rac guanine nucleotide exchange factors
            promoting Lgl1 phosphorylation in glioblastoma.
REFERENCE   3  (bases 1 to 4583)
  AUTHORS   Kachroo,P., Hecker,J., Chawes,B.L., Ahluwalia,T.S., Cho,M.H.,
            Qiao,D., Kelly,R.S., Chu,S.H., Virkud,Y.V., Huang,M., Barnes,K.C.,
            Burchard,E.G., Eng,C., Hu,D., Celedon,J.C., Daya,M., Levin,A.M.,
            Gui,H., Williams,L.K., Forno,E., Mak,A.C.Y., Avila,L.,
            Soto-Quiros,M.E., Cloutier,M.M., Acosta-Perez,E., Canino,G.,
            Bonnelykke,K., Bisgaard,H., Raby,B.A., Lange,C., Weiss,S.T. and
            Lasky-Su,J.A.
  CONSRTM   National Heart, Lung, and Blood Institute Trans-Omics for Precision
            Medicine (TOPMed) Consortium
  TITLE     Whole Genome Sequencing Identifies CRISPLD2 as a Lung Function Gene
            in Children With Asthma
  JOURNAL   Chest 156 (6), 1068-1079 (2019)
   PUBMED   31557467
  REMARK    GeneRIF: Our findings suggest that using specific asthma
            characteristics, such as airway hyperresponsiveness, can help
            identify more genetically homogeneous asthma subgroups with
            genotype-phenotype associations that may not be observed in all
            children with asthma. CRISPLD2 also may be important for baseline
            lung function in individuals with asthma who also may have airway
            hyperresponsiveness.
REFERENCE   4  (bases 1 to 4583)
  AUTHORS   Chiquet,B.T., Yuan,Q., Swindell,E.C., Maili,L., Plant,R., Dyke,J.,
            Boyer,R., Teichgraeber,J.F., Greives,M.R., Mulliken,J.B., Letra,A.,
            Blanton,S.H. and Hecht,J.T.
  TITLE     Knockdown of Crispld2 in zebrafish identifies a novel network for
            nonsyndromic cleft lip with or without cleft palate candidate genes
  JOURNAL   Eur J Hum Genet 26 (10), 1441-1450 (2018)
   PUBMED   29899370
  REMARK    GeneRIF: we demonstrated that knockdown of Crispld2 in zebrafish
            alters neural crest cell migration patterns resulting in abnormal
            jaw and palate development. In this study, we performed RNA
            profiling in zebrafish embryos and identified 249 differentially
            expressed genes following knockdown of Crispld2.
REFERENCE   5  (bases 1 to 4583)
  AUTHORS   Ge,X., Shi,Q.M., Ding,Z., Ju,Q., Wang,H., Wang,Q., Li,M.X.,
            Chen,G., Wang,H.X. and Xu,L.C.
  TITLE     Association Between CRISPLD2 Polymorphisms and the Risk of
            Nonsyndromic Clefts of the Lip and/or Palate: A Meta-analysis
  JOURNAL   Cleft Palate Craniofac J 55 (3), 328-334 (2018)
   PUBMED   29437515
  REMARK    GeneRIF: CRISPLD2 rs4783099 was associated with cleft lip and/or
            palate (CL/P) statistically (OR = 3.18, P < .01). Compared to
            genotype TT, genotypes CC and CT were correlated significantly (OR
            = 2.04, P = .04) with CL/P. No evidence showed an association
            between genetic variation at the CRISPLD2 locus and cleft palate
            only (CP).
REFERENCE   6  (bases 1 to 4583)
  AUTHORS   Wang,Z.Q., Xing,W.M., Fan,H.H., Wang,K.S., Zhang,H.K., Wang,Q.W.,
            Qi,J., Yang,H.M., Yang,J., Ren,Y.N., Cui,S.J., Zhang,X., Liu,F.,
            Lin,D.H., Wang,W.H., Hoffmann,M.K. and Han,Z.G.
  TITLE     The novel lipopolysaccharide-binding protein CRISPLD2 is a critical
            serum protein to regulate endotoxin function
  JOURNAL   J Immunol 183 (10), 6646-6656 (2009)
   PUBMED   19864597
  REMARK    GeneRIF: present work introduces mammal CRISPLD2 as a major serum
            protein that acts as a natural LPS antagonist and promises to be of
            considerable preventative value against endotoxic shock.
REFERENCE   7  (bases 1 to 4583)
  AUTHORS   Gudbjartsson,D.F., Walters,G.B., Thorleifsson,G., Stefansson,H.,
            Halldorsson,B.V., Zusmanovich,P., Sulem,P., Thorlacius,S.,
            Gylfason,A., Steinberg,S., Helgadottir,A., Ingason,A.,
            Steinthorsdottir,V., Olafsdottir,E.J., Olafsdottir,G.H.,
            Jonsson,T., Borch-Johnsen,K., Hansen,T., Andersen,G., Jorgensen,T.,
            Pedersen,O., Aben,K.K., Witjes,J.A., Swinkels,D.W., den Heijer,M.,
            Franke,B., Verbeek,A.L., Becker,D.M., Yanek,L.R., Becker,L.C.,
            Tryggvadottir,L., Rafnar,T., Gulcher,J., Kiemeney,L.A., Kong,A.,
            Thorsteinsdottir,U. and Stefansson,K.
  TITLE     Many sequence variants affecting diversity of adult human height
  JOURNAL   Nat Genet 40 (5), 609-615 (2008)
   PUBMED   18391951
REFERENCE   8  (bases 1 to 4583)
  AUTHORS   Chiquet,B.T., Lidral,A.C., Stal,S., Mulliken,J.B., Moreno,L.M.,
            Arcos-Burgos,M., Valencia-Ramirez,C., Blanton,S.H. and Hecht,J.T.
  TITLE     CRISPLD2: a novel NSCLP candidate gene
  JOURNAL   Hum Mol Genet 16 (18), 2241-2248 (2007)
   PUBMED   17616516
  REMARK    GeneRIF: CRISPLD2 is expressed in the mandible, palate and
            nasopharynx regions during craniofacial development at E13.5-E17.5,
            respectively. Altogether, these data suggest that genetic variation
            in CRISPLD2 has a role in the etiology of NSCLP.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Erratum:[Hum Mol Genet. 2008 Apr 1;17(7):1061. Arco-Burgos,
            Mauricio [corrected to Arcos-Burgos, Mauricio]]
REFERENCE   9  (bases 1 to 4583)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   10 (bases 1 to 4583)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DB095807.1, DA325436.1, AL136861.1, AC025280.5, BQ015624.1 and
            DB260709.1.
            
            On Nov 22, 2018 this sequence version replaced NM_031476.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AL136861.1, HM005620.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000262424.10/ ENSP00000262424.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-31                DB095807.1         5-35
            32-149              DA325436.1         32-149
            150-693             AL136861.1         131-674
            694-694             AC025280.5         91132-91132         c
            695-1947            AL136861.1         676-1928
            1948-2328           BQ015624.1         179-559
            2329-2797           DB260709.1         89-557
            2798-3772           AL136861.1         2779-3753
            3773-3778           AC025280.5         31925-31930         c
            3779-4583           AL136861.1         3754-4558
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q24.1"
     gene            1..4583
                     /gene="CRISPLD2"
                     /gene_synonym="CRISP11; LCRISP2; LGL1"
                     /note="cysteine rich secretory protein LCCL domain
                     containing 2"
                     /db_xref="GeneID:83716"
                     /db_xref="HGNC:HGNC:25248"
                     /db_xref="MIM:612434"
     exon            1..149
                     /gene="CRISPLD2"
                     /gene_synonym="CRISP11; LCRISP2; LGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            150..463
                     /gene="CRISPLD2"
                     /gene_synonym="CRISP11; LCRISP2; LGL1"
                     /inference="alignment:Splign:2.1.0"
     CDS             224..1717
                     /gene="CRISPLD2"
                     /gene_synonym="CRISP11; LCRISP2; LGL1"
                     /note="CRISP-11; cysteine-rich secretory protein 11;
                     testis secretory sperm-binding protein Li 207a; LCCL
                     domain-containing cysteine-rich secretory protein 2;
                     trypsin inhibitor; late gestation lung 1"
                     /codon_start=1
                     /product="cysteine-rich secretory protein LCCL
                     domain-containing 2 precursor"
                     /protein_id="NP_113664.1"
                     /db_xref="CCDS:CCDS10949.1"
                     /db_xref="GeneID:83716"
                     /db_xref="HGNC:HGNC:25248"
                     /db_xref="MIM:612434"
                     /translation="MSCVLGGVIPLGLLFLVCGSQGYLLPNVTLLEELLSKYQHNESH
                     SRVRRAIPREDKEEILMLHNKLRGQVQPQASNMEYMTWDDELEKSAAAWASQCIWEHG
                     PTSLLVSIGQNLGAHWGRYRSPGFHVQSWYDEVKDYTYPYPSECNPWCPERCSGPMCT
                     HYTQIVWATTNKIGCAVNTCRKMTVWGEVWENAVYFVCNYSPKGNWIGEAPYKNGRPC
                     SECPPSYGGSCRNNLCYREETYTPKPETDEMNEVETAPIPEENHVWLQPRVMRPTKPK
                     KTSAVNYMTQVVRCDTKMKDRCKGSTCNRYQCPAGCLNHKAKIFGTLFYESSSSICRA
                     AIHYGILDDKGGLVDITRNGKVPFFVKSERHGVQSLSKYKPSSSFMVSKVKVQDLDCY
                     TTVAQLCPFEKPATHCPRIHCPAHCKDEPSYWAPVFGTNIYADTSSICKTAVHAGVIS
                     NESGGDVDVMPVDKKKTYVGSLRNGVQSESLGTPRDGKAFRIFAVRQ"
     exon            464..582
                     /gene="CRISPLD2"
                     /gene_synonym="CRISP11; LCRISP2; LGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            583..715
                     /gene="CRISPLD2"
                     /gene_synonym="CRISP11; LCRISP2; LGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            716..831
                     /gene="CRISPLD2"
                     /gene_synonym="CRISP11; LCRISP2; LGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            832..932
                     /gene="CRISPLD2"
                     /gene_synonym="CRISP11; LCRISP2; LGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            933..1076
                     /gene="CRISPLD2"
                     /gene_synonym="CRISP11; LCRISP2; LGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1077..1137
                     /gene="CRISPLD2"
                     /gene_synonym="CRISP11; LCRISP2; LGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1138..1204
                     /gene="CRISPLD2"
                     /gene_synonym="CRISP11; LCRISP2; LGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1205..1335
                     /gene="CRISPLD2"
                     /gene_synonym="CRISP11; LCRISP2; LGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1336..1379
                     /gene="CRISPLD2"
                     /gene_synonym="CRISP11; LCRISP2; LGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1380..1452
                     /gene="CRISPLD2"
                     /gene_synonym="CRISP11; LCRISP2; LGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1453..1528
                     /gene="CRISPLD2"
                     /gene_synonym="CRISP11; LCRISP2; LGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1529..1662
                     /gene="CRISPLD2"
                     /gene_synonym="CRISP11; LCRISP2; LGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1663..4583
                     /gene="CRISPLD2"
                     /gene_synonym="CRISP11; LCRISP2; LGL1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4565..4570
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CRISPLD2"
                     /gene_synonym="CRISP11; LCRISP2; LGL1"
                     /note="hexamer: AATAAA"
     polyA_site      4583
                     /gene="CRISPLD2"
                     /gene_synonym="CRISP11; LCRISP2; LGL1"
                     /note="major polyA site"
ORIGIN      
        1 actcgcatcc cagggcgtct ccggctgctc ctattgagct gtctgctcgc tgtgcccgct
       61 gtgcctgctg tgcccgcgct gtcgccgctg ctaccgcgtc tgctggacgc gggagacgcc
      121 agcgagctgg tgattggagc cctgcggaga gctcaagcgc ccagctctgc ccgaggagcc
      181 caggctgccc cgtgagtccc atagttgctg caggagtgga gccatgagct gcgtcctggg
      241 tggtgtcatc cccttggggc tgctgttcct ggtctgcgga tcccaaggct acctcctgcc
      301 caacgtcact ctcttagagg agctgctcag caaataccag cacaacgagt ctcactcccg
      361 ggtccgcaga gccatcccca gggaggacaa ggaggagatc ctcatgctgc acaacaagct
      421 tcggggccag gtgcagcctc aggcctccaa catggagtac atgacctggg atgacgaact
      481 ggagaagtct gctgcagcgt gggccagtca gtgcatctgg gagcacgggc ccaccagtct
      541 gctggtgtcc atcgggcaga acctgggcgc tcactggggc aggtatcgct ctccggggtt
      601 ccatgtgcag tcctggtatg acgaggtgaa ggactacacc tacccctacc cgagcgagtg
      661 caacccctgg tgtccagaga ggtgctcggg gcccatgtgc acgcactaca cacagatagt
      721 ttgggccacc accaacaaga tcggttgtgc tgtgaacacc tgccggaaga tgactgtctg
      781 gggagaagtt tgggagaacg cggtctactt tgtctgcaat tattctccaa aggggaactg
      841 gattggagaa gccccctaca agaatggccg gccctgctct gagtgcccac ccagctatgg
      901 aggcagctgc aggaacaact tgtgttaccg agaagaaacc tacactccaa aacctgaaac
      961 ggacgagatg aatgaggtgg aaacggctcc cattcctgaa gaaaaccatg tttggctcca
     1021 accgagggtg atgagaccca ccaagcccaa gaaaacctct gcggtcaact acatgaccca
     1081 agtcgtcaga tgtgacacca agatgaagga caggtgcaaa gggtccacgt gtaacaggta
     1141 ccagtgccca gcaggctgcc tgaaccacaa ggcgaagatc tttggaactc tgttctatga
     1201 aagctcgtct agcatatgcc gcgccgccat ccactacggg atcctggatg acaagggagg
     1261 cctggtggat atcaccagga acgggaaggt ccccttcttc gtgaagtctg agagacacgg
     1321 cgtgcagtcc ctcagcaaat acaaaccttc cagctcattc atggtgtcaa aagtgaaagt
     1381 gcaggatttg gactgctaca cgaccgttgc tcagctgtgc ccgtttgaaa agccagcaac
     1441 tcactgccca agaatccatt gtccggcaca ctgcaaagac gaaccttcct actgggctcc
     1501 ggtgtttgga accaacatct atgcagatac ctcaagcatc tgcaagacag ccgtgcacgc
     1561 gggagtcatc agcaacgaga gtgggggtga cgtggacgtg atgcccgtgg ataaaaagaa
     1621 gacctacgtg ggctcgctca ggaatggagt tcagtctgaa agcctgggga ctcctcggga
     1681 tggaaaggcc ttccggatct ttgctgtcag gcagtgaatt tccagcacca ggggagaagg
     1741 ggcgtcttca ggagggcttc ggggttttgc ttttattttt attttgtcat tgcggggtat
     1801 atggagagtc aggaaacttc ctttgactga tgttcagtgt ccatcacttt gtggcctgtg
     1861 ggtgaggtga catctcatcc cctcactgaa gcaacagcat cccaaggtgc tcagccggac
     1921 tccctggtgc ctgatcctgc tggggcctgg gggtctccat ctggacgtcc tctctccttt
     1981 agagatctga gctgtctctt aaaggggaca gttgcccaaa atgttccttg ctatgtgttc
     2041 ttctgttggt ggaggaagtt gatttcaacc tccctgccaa aagaacaaac catttgaagc
     2101 tcacaattgt gaagcattca cggcgtcgga agaggccttt tgagcaagcg ccaatgagtt
     2161 tcaggaatga agtagaaggt agttatttaa aaataaaaaa cacagtccgt ccctaccaat
     2221 agaggaaaat ggttttaatg tttgctggtc agacagacaa atgggctaga gtaagagggc
     2281 tgcgggtatg agagaccccg gctccgccct ggcacgtgtc cttgctggcg gcccgccaca
     2341 ggcccccttc aatggccgca ttcaggatgg ctctatacac agcagtgctg gtttatgtag
     2401 agttcagcag tcacttcaga gatgtatctt gtctttgtca ggcccttcgt cttcatggcc
     2461 cacctgtttt ctgccgtgac ctttggtccc attgaggact aaggatcggg accctttctt
     2521 taccccctac ccgttgtggc tcccaccctg cctcggactg gtttacgtgt cctggttcac
     2581 acccaggact tttctttgca agcgaacctg tttgaagccc aagtcttaac tcctggtctc
     2641 gtaaggttcc actgagacga gatgtctgag aacaaccaaa gaaggcctgc tctttgctgc
     2701 ttttaaaaaa tgacaattaa atgtgcagat tccccacgca cccgatgacc tattttttca
     2761 gccgtgggag gaatggagtc tttggtacat tcctcaccga ggttagcagc tcagtttgtg
     2821 gttatgaaac cgtctgtggc ctcatgacag cgagagatgg gaatacacta gaaggatctc
     2881 ttttcctgtt ttcgtgaaac gactcttgcc aaacgttccc gaggcgccaa ggagtgtagt
     2941 acaccctggc tgccatcact ctataaaagt gcttcatgag cccagaccaa aagcccacag
     3001 tgaaatgaag tacccttttg taaatagcat ttttttgcag aaggtgaaaa ttccactctc
     3061 taccaccggg ccagccaata gatcactttg gtgaatgcta gtttcaaatt tgattcaaaa
     3121 tatttcttag gtgaaagaac tagcagaaag tcaaaaacta agatactgta gactggacaa
     3181 gaaattctac ctgggcacct aggtgatgcc ttctttcttt gattgccttt ctaataaatg
     3241 cagaatctga aggtaaatag gtttaaaaca aaacaaaaac ccaccccttt aaggagttgg
     3301 taaaaagcag ttcaactctt agcttgactg agctaaaatt cacaggacta cgtgctttgt
     3361 gcattgtagt ctagtcgtaa ttcataggta ctgactcctc agccccaaat gtcggagagg
     3421 aagaattcgg tcagcctgtc aggtcgtgag tccagttacc accaaacatc tgggaaactt
     3481 ctgggtgctg ggtgctctgc tgctggactt ttgtggctgt gtctgtgtct gcaagataaa
     3541 ttagatcgcc ctgtggggtt tgcagaatta gtgaagggtc caggacgatc ccagtgggct
     3601 cgcttccaaa gcatcccact caagggagac ttgaaacttc cagtgtgagt tgaccccatc
     3661 atttaaaaat aaagtccccg ggttccttaa tgcctccttc actgggcctt cctagcagga
     3721 tagaaagtcc ttgcccagag caggacctgg ctgtcttttt tttttttttt ttttttttcc
     3781 cgagaccaag tttcactctg ttgcccaagg tagagtgcag tggcgtgatc tctgctcatt
     3841 gcaactgccg cctcccgggt tcaagcaatt ctcatgcatc agcctcccaa gtacctggga
     3901 ctacaggcgt gagctaccat gcccggctaa tttttgtatt tttagtagag atggggtttc
     3961 attatgttgg ccaggctggt ctcgaactcc ttacctcagg tgatccaccc accttggcct
     4021 cccgaagtgc tgggattaca ggcatgagcc actgcgcccg gccatggacc tggctgtctt
     4081 tatcatcccc acaaacattt tgaaactgga atatttgtct tcagaaaatg gaaacaagac
     4141 tataaatgat aagccctgtc cctagcacca cctctcctgt gtgtggaata gaggcccctc
     4201 gtgctaccaa cacttaccct gtgtttaaaa agatcttgta ccaagccaac ggcgttcctg
     4261 gctctcctgc ccacaggatg aacattttcg gcttccttag gagttttgcc ctaccgtatt
     4321 ccaaagcgtg tgctggtttc tcatattgtc tgtaggctca ctcagcccgc agtttatgtg
     4381 tgtgcttttt tctatgaaaa atgatgtatt ttgctacttc ctgtgtacaa agttttattg
     4441 taaatgtttt ttgtgctttg catgaacagg ggccacgttg ttgcaattgt ttcagtagaa
     4501 ctggtttgat ttctaaaatg ttcctgtaac atatctttta tgaacaaatc tgaacaattt
     4561 gtgaaataaa acattgaaaa cca
//
