LOCUS       NM_031461               4297 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens cysteine rich secretory protein LCCL domain containing
            1 (CRISPLD1), transcript variant 1, mRNA.
ACCESSION   NM_031461
VERSION     NM_031461.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Khadjeh,S., Hindmarsh,V., Weber,F., Cyganek,L., Vidal,R.O.,
            Torkieh,S., Streckfuss-Bomeke,K., Lbik,D., Tiburcy,M.,
            Mohamed,B.A., Bonn,S., Toischer,K. and Hasenfuss,G.
  TITLE     CRISPLD1: a novel conserved target in the transition to human heart
            failure
  JOURNAL   Basic Res Cardiol 115 (3), 27 (2020)
   PUBMED   32146539
  REMARK    GeneRIF: CRISPLD1: a novel conserved target in the transition to
            human heart failure; findings provide new pathophysiological data
            on Ca(2+) regulation in the transition to failure and novel
            candidate genes with promising potential for therapeutic
            interventions
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Chiquet,B.T., Henry,R., Burt,A., Mulliken,J.B., Stal,S.,
            Blanton,S.H. and Hecht,J.T.
  TITLE     Nonsyndromic cleft lip and palate: CRISPLD genes and the folate
            gene pathway connection
  JOURNAL   Birth Defects Res A Clin Mol Teratol 91 (1), 44-49 (2011)
   PUBMED   21254358
  REMARK    GeneRIF: These novel findings suggest that CRISPLD1 plays a role in
            Nonsyndromic cleft lip and palate (NSCLP) through the interaction
            with CRISPLD2 and folate pathway genes.
REFERENCE   3  (bases 1 to 4297)
  AUTHORS   Gonzalez-Begne,M., Lu,B., Han,X., Hagen,F.K., Hand,A.R.,
            Melvin,J.E. and Yates,J.R.
  TITLE     Proteomic analysis of human parotid gland exosomes by
            multidimensional protein identification technology (MudPIT)
  JOURNAL   J Proteome Res 8 (3), 1304-1314 (2009)
   PUBMED   19199708
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BP357192.1, BC020514.1, AC100782.2 and BU790187.1.
            
            On Nov 23, 2018 this sequence version replaced NM_031461.5.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AL834301.1, SRR1660805.247975.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000262207.9/ ENSP00000262207.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-103               BP357192.1         33-135
            104-3045            BC020514.1         1-2942
            3046-3703           AC100782.2         111120-111777       c
            3704-4297           BU790187.1         6-599               c
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q21.13"
     gene            1..4297
                     /gene="CRISPLD1"
                     /gene_synonym="CRISP-10; CRISP10; LCRISP1"
                     /note="cysteine rich secretory protein LCCL domain
                     containing 1"
                     /db_xref="GeneID:83690"
                     /db_xref="HGNC:HGNC:18206"
     exon            1..416
                     /gene="CRISPLD1"
                     /gene_synonym="CRISP-10; CRISP10; LCRISP1"
                     /inference="alignment:Splign:2.1.0"
     exon            417..736
                     /gene="CRISPLD1"
                     /gene_synonym="CRISP-10; CRISP10; LCRISP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             479..1981
                     /gene="CRISPLD1"
                     /gene_synonym="CRISP-10; CRISP10; LCRISP1"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; CocoaCrisp; trypsin inhibitor Hl; cysteine-rich
                     secretory protein 10; LCCL domain-containing cysteine-rich
                     secretory protein 1"
                     /codon_start=1
                     /product="cysteine-rich secretory protein LCCL
                     domain-containing 1 isoform 1 precursor"
                     /protein_id="NP_113649.1"
                     /db_xref="CCDS:CCDS6219.1"
                     /db_xref="GeneID:83690"
                     /db_xref="HGNC:HGNC:18206"
                     /translation="MKCTAREWLRVTTVLFMARAIPAMVVPNATLLEKLLEKYMDEDG
                     EWWIAKQRGKRAITDNDMQSILDLHNKLRSQVYPTASNMEYMTWDVELERSAESWAES
                     CLWEHGPASLLPSIGQNLGAHWGRYRPPTFHVQSWYDEVKDFSYPYEHECNPYCPFRC
                     SGPVCTHYTQVVWATSNRIGCAINLCHNMNIWGQIWPKAVYLVCNYSPKGNWWGHAPY
                     KHGRPCSACPPSFGGGCRENLCYKEGSDRYYPPREEETNEIERQQSQVHDTHVRTRSD
                     DSSRNEVISAQQMSQIVSCEVRLRDQCKGTTCNRYECPAGCLDSKAKVIGSVHYEMQS
                     SICRAAIHYGIIDNDGGWVDITRQGRKHYFIKSNRNGIQTIGKYQSANSFTVSKVTVQ
                     AVTCETTVEQLCPFHKPASHCPRVYCPRNCMQANPHYARVIGTRVYSDLSSICRAAVH
                     AGVVRNHGGYVDVMPVDKRKTYIASFQNGIFSESLQNPPGGKAFRVFAVV"
     exon            737..855
                     /gene="CRISPLD1"
                     /gene_synonym="CRISP-10; CRISP10; LCRISP1"
                     /inference="alignment:Splign:2.1.0"
     exon            856..988
                     /gene="CRISPLD1"
                     /gene_synonym="CRISP-10; CRISP10; LCRISP1"
                     /inference="alignment:Splign:2.1.0"
     exon            989..1104
                     /gene="CRISPLD1"
                     /gene_synonym="CRISP-10; CRISP10; LCRISP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1105..1205
                     /gene="CRISPLD1"
                     /gene_synonym="CRISP-10; CRISP10; LCRISP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1206..1346
                     /gene="CRISPLD1"
                     /gene_synonym="CRISP-10; CRISP10; LCRISP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1347..1407
                     /gene="CRISPLD1"
                     /gene_synonym="CRISP-10; CRISP10; LCRISP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1408..1474
                     /gene="CRISPLD1"
                     /gene_synonym="CRISP-10; CRISP10; LCRISP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1475..1605
                     /gene="CRISPLD1"
                     /gene_synonym="CRISP-10; CRISP10; LCRISP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1606..1649
                     /gene="CRISPLD1"
                     /gene_synonym="CRISP-10; CRISP10; LCRISP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1650..1722
                     /gene="CRISPLD1"
                     /gene_synonym="CRISP-10; CRISP10; LCRISP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1723..1798
                     /gene="CRISPLD1"
                     /gene_synonym="CRISP-10; CRISP10; LCRISP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1799..1929
                     /gene="CRISPLD1"
                     /gene_synonym="CRISP-10; CRISP10; LCRISP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1930..4297
                     /gene="CRISPLD1"
                     /gene_synonym="CRISP-10; CRISP10; LCRISP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2309..2314
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CRISPLD1"
                     /gene_synonym="CRISP-10; CRISP10; LCRISP1"
                     /note="hexamer: AATGAA"
     polyA_site      2327
                     /gene="CRISPLD1"
                     /gene_synonym="CRISP-10; CRISP10; LCRISP1"
                     /note="major polyA site"
     regulatory      3017..3022
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CRISPLD1"
                     /gene_synonym="CRISP-10; CRISP10; LCRISP1"
                     /note="hexamer: ATTAAA"
     polyA_site      3039
                     /gene="CRISPLD1"
                     /gene_synonym="CRISP-10; CRISP10; LCRISP1"
                     /note="major polyA site"
     regulatory      4217..4222
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CRISPLD1"
                     /gene_synonym="CRISP-10; CRISP10; LCRISP1"
                     /note="hexamer: AATAAA"
     polyA_site      4240
                     /gene="CRISPLD1"
                     /gene_synonym="CRISP-10; CRISP10; LCRISP1"
     regulatory      4266..4271
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CRISPLD1"
                     /gene_synonym="CRISP-10; CRISP10; LCRISP1"
                     /note="hexamer: ATTAAA"
     polyA_site      4297
                     /gene="CRISPLD1"
                     /gene_synonym="CRISP-10; CRISP10; LCRISP1"
ORIGIN      
        1 ggaagcgcta ggcgcctggt tctgcgcgta ctggctgtac ggagcaggag caagaggtcg
       61 ccgccagcct ccgccgccga gcctcgttcg tgtccccgcc cctcgctcct gcagctactg
      121 ctcagaaacg ctggggcgcc caccctggca gactaacgaa gcagctccct tcccacccca
      181 actgcaggtc taattttgga cgctttgcct gccatttctt ccaggttgag ggagccgcag
      241 aggcggaggc tcgcgtattc ctgcagtcag cacccacgtc gcccccggac gctcggtgct
      301 caggcccttc gcgagcgggg ctctccgtct gcggtccctt gtgaaggctc tgggcggctg
      361 cagaggccgg ccgtccggtt tggctcacct ctcccaggaa acttcacact ggagagccaa
      421 aaggagtgga agagcctgtc ttggagattt tcctggggaa atcctgaggt cattcattat
      481 gaagtgtacc gcgcgggagt ggctcagagt aaccacagtg ctgttcatgg ctagagcaat
      541 tccagccatg gtggttccca atgccacttt attggagaaa cttttggaaa aatacatgga
      601 tgaggatggt gagtggtgga tagccaaaca acgagggaaa agggccatca cagacaatga
      661 catgcagagt attttggacc ttcataataa attacgaagt caggtgtatc caacagcctc
      721 taatatggag tatatgacat gggatgtaga gctggaaaga tctgcagaat cctgggctga
      781 aagttgcttg tgggaacatg gacctgcaag cttgcttcca tcaattggac agaatttggg
      841 agcacactgg ggaagatata ggcccccgac gtttcatgta caatcgtggt atgatgaagt
      901 gaaagacttt agctacccat atgaacatga atgcaaccca tattgtccat tcaggtgttc
      961 tggccctgta tgtacacatt atacacaggt cgtgtgggca actagtaaca gaatcggttg
     1021 tgccattaat ttgtgtcata acatgaacat ctgggggcag atatggccca aagctgtcta
     1081 cctggtgtgc aattactccc caaagggaaa ctggtggggc catgcccctt acaaacatgg
     1141 gcggccctgt tctgcttgcc cacctagttt tggagggggc tgtagagaaa atctgtgcta
     1201 caaagaaggg tcagacaggt attatccccc tcgagaagag gaaacaaatg aaatagaacg
     1261 acagcagtca caagtccatg acacccatgt ccggacaaga tcagatgata gtagcagaaa
     1321 tgaagtcata agcgcacagc aaatgtccca aattgtttct tgtgaagtaa gattaagaga
     1381 tcagtgcaaa ggaacaacct gcaataggta cgaatgtcct gctggctgtt tggatagtaa
     1441 agctaaagtt attggcagtg tacattatga aatgcaatcc agcatctgta gagctgcaat
     1501 tcattatggt ataatagaca atgatggtgg ctgggtagat atcactagac aaggaagaaa
     1561 gcattatttc atcaagtcca atagaaatgg tattcaaaca attggcaaat atcagtctgc
     1621 taattccttc acagtctcta aagtaacagt tcaggctgtg acttgtgaaa caactgtgga
     1681 acagctctgt ccatttcata agcctgcttc acattgccca agagtatact gtcctcgtaa
     1741 ctgtatgcaa gcaaatccac attatgctcg tgtaattgga actcgagttt attctgatct
     1801 gtccagtatc tgcagagcag cagtacatgc tggagtggtt cgaaatcacg gtggttatgt
     1861 tgatgtaatg cctgtggaca aaagaaagac ctacattgct tcttttcaga atggaatctt
     1921 ctcagaaagt ttacagaatc ctccaggagg aaaggcattc agagtgtttg ctgttgtgtg
     1981 aaactgaata cttggaagag gaccataaag actattccaa atgcaatatt tctgaatttt
     2041 gtataaaact gtaacattac tgtacagagt acatcaacta ttttcagccc aaaaaggtgc
     2101 caaatgcata taaatcttga taaacaaagt ctataaaata aaacatggga cattagcttt
     2161 gggaaaagta atgaaaatat aatggtttta gaaatcctgt gttaaatatt gctatatttt
     2221 cttagcagtt atttctacag ttaattacat agtcatgatt gttctacgtt tcatatatta
     2281 tatggtgctt tgtatatgcc actaataaaa tgaatctaaa cattgaatgt gaatggccct
     2341 cagaaaatca tctagtgcat ttaaaaataa tcgactctaa aactgaaaga aaccttatca
     2401 cattttcccc agttcaatgc tatgccatta ccaactccaa ataatctcaa ataattttcc
     2461 acttaataac tgtaaagttt ttttctgtta atttaggcat atagaatatt aaattctgat
     2521 attgcacttc ttattttata taaaataatc ctttaatatc caaatgaatc tgttaaaatg
     2581 tttgattcct tgggaatggc cttaaaaata aatgtaataa agtcagagtg gtggtatgaa
     2641 aacattccta gtgatcatgt agtaaatgta gggttaagca tggacagcca gagctttcta
     2701 tgtactgtta aaattgaggt cacatatttt cttttgtatc ctggcaaata ctcctgcagg
     2761 ccaggaagta taatagcaaa aagttgaaca aagatgaact aatgtattac attaccattg
     2821 ccactgattt ttttttaaat ggtaaatgac cttgtatata aatattgcca tatcatggta
     2881 cctataatgg tgatatattt gtttctatga aaaatgtatt gtgctttgat actaaaaatc
     2941 tgtaaaatgt tagttttggt aatttttttt ctgctggtgg atttacatat taaatttttt
     3001 ctgctggtgg ataaacatta aaattaatca tgtttcaaag ttttattttc agttcctttt
     3061 gcatgcctat tttgatttag aaatcacttt aagataaatg aacaaaatta ttgtaagtct
     3121 tctaaacttg gtttattgac gttagtataa ataacataca ataccagatg tctacaaaat
     3181 cgacctgatt atttaggtat ttgtatgtga aagagaaaca catatttaga aacacagcaa
     3241 gggagatttt gaataaagag agagatgaat ttataaagta ggaaaaaaag aatctgaaag
     3301 atacaaagtg attataaaag aattgacagg acaataaata cttcaaatac ttttaggaag
     3361 taaatgagta ggagtttagg agggataaac gagataaatt ccatgcaatg atcaaggaaa
     3421 gcaagagagc agataataca aaagaaaaag aagaaggttc acataaatag cttctggagt
     3481 gcaaattata aagacatcaa catttttgat catataataa gtactggctt acagctaagt
     3541 ggttccaaat actggtaatt aaaacaatga tgtatacgtt aaagattgca aaacttaaaa
     3601 gcagattttt atgggaactt ttttcgaaag gatacatgac caccttctta aatagtatga
     3661 ctttacatag actctttttc tggtccttac tgctcctccc acaacaggga agcccgatca
     3721 gttctgtcag tataaggttt tcttctattt gcccttagtt tagatctcca tcatcaattc
     3781 cacttactat tgtaatacct tcagctggag cctgtgtctt tctccattcc tttttaatac
     3841 attcgtattt cccagcccca gaacccttga aattaactct tccatgagtt ttcattgctg
     3901 aagaaaaacc aaacattctt gtaaaaacat ttaagaactt ccatagtctt gttcatattg
     3961 tttacctatc ttctacacaa ggcgtgtcct tgggtaagtt atttaacttc aatttacctt
     4021 acctgtaaaa tggaacaaat aatatctcct taagattgct ctacgaatta attgacacat
     4081 tatgtgtgat gtacttaaag gaataataaa tgcataatat gaggtcagtg gacgccattt
     4141 tctgttataa tcatttttgt tatctaattt ggggaaacta ttcatctgtc atatactatt
     4201 gatcctttgg cacttaaata aatgtgaaat cctccaggaa ttttcctgtg attctccttg
     4261 taagaattaa atgctctcta atatgatatc cctttaa
//
