LOCUS       NM_006061               2152 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens cysteine rich secretory protein 3 (CRISP3), transcript
            variant 1, mRNA.
ACCESSION   NM_006061
VERSION     NM_006061.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Wang,Y., Zhang,Y. and Qi,X.
  TITLE     EP300 promotes tumor stemness via epigenetic activation of CRISP3
            leading to lobaplatin resistance in triple-negative breast cancer
  JOURNAL   Hum Cell 37 (5), 1475-1488 (2024)
   PUBMED   38879857
  REMARK    GeneRIF: EP300 promotes tumor stemness via epigenetic activation of
            CRISP3 leading to lobaplatin resistance in triple-negative breast
            cancer.
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 2152)
  AUTHORS   Wang,Y., Sheng,N., Xie,Y., Chen,S., Lu,J., Zhang,Z., Shan,Q.,
            Wu,D., Zheng,G., Li,M., Zheng,Y. and Fan,S.
  TITLE     Low expression of CRISP3 predicts a favorable prognosis in patients
            with mammary carcinoma
  JOURNAL   J Cell Physiol 234 (8), 13629-13638 (2019)
   PUBMED   30609035
  REMARK    GeneRIF: In this study, we found obvious differences in the
            expression of CRISP3 between different mammary carcinoma cells,
            which is related to the prognosis of patients with mammary
            carcinoma
REFERENCE   4  (bases 1 to 2152)
  AUTHORS   Belardin,L., Camargo,M., Intasqui,P., Antoniassi,M., Fraietta,R.
            and Bertolla,R.
  TITLE     Cysteine-rich secretory protein 3: inflammation role in adult
            varicocoele
  JOURNAL   Andrology 7 (1), 53-61 (2019)
   PUBMED   30354034
  REMARK    GeneRIF: in the presence of varicocoele, there is a marked increase
            in seminal CRISP-3 levels. Surgical intervention (varicocelectomy)
            decreases CRISP-3 levels and improves semen quality.
REFERENCE   5  (bases 1 to 2152)
  AUTHORS   Da Ros,V.G., Munoz,M.W., Battistone,M.A., Brukman,N.G.,
            Carvajal,G., Curci,L., Gomez-ElIas,M.D., Cohen,D.B. and
            Cuasnicu,P.S.
  TITLE     From the epididymis to the egg: participation of CRISP proteins in
            mammalian fertilization
  JOURNAL   Asian J Androl 17 (5), 711-715 (2015)
   PUBMED   26112483
  REMARK    Review article
REFERENCE   6  (bases 1 to 2152)
  AUTHORS   Udby,L., Cowland,J.B., Johnsen,A.H., Sorensen,O.E., Borregaard,N.
            and Kjeldsen,L.
  TITLE     An ELISA for SGP28/CRISP-3, a cysteine-rich secretory protein in
            human neutrophils, plasma, and exocrine secretions
  JOURNAL   J Immunol Methods 263 (1-2), 43-55 (2002)
   PUBMED   12009203
REFERENCE   7  (bases 1 to 2152)
  AUTHORS   Friess,H., Ding,J., Kleeff,J., Liao,Q., Berberat,P.O., Hammer,J.
            and Buchler,M.W.
  TITLE     Identification of disease-specific genes in chronic pancreatitis
            using DNA array technology
  JOURNAL   Ann Surg 234 (6), 769-78 (2001)
   PUBMED   11729383
REFERENCE   8  (bases 1 to 2152)
  AUTHORS   Cowland,J.B. and Borregaard,N.
  TITLE     The individual regulation of granule protein mRNA levels during
            neutrophil maturation explains the heterogeneity of neutrophil
            granules
  JOURNAL   J Leukoc Biol 66 (6), 989-995 (1999)
   PUBMED   10614782
REFERENCE   9  (bases 1 to 2152)
  AUTHORS   Kratzschmar,J., Haendler,B., Eberspaecher,U., Roosterman,D.,
            Donner,P. and Schleuning,W.D.
  TITLE     The human cysteine-rich secretory protein (CRISP) family. Primary
            structure and tissue distribution of CRISP-1, CRISP-2 and CRISP-3
  JOURNAL   Eur J Biochem 236 (3), 827-836 (1996)
   PUBMED   8665901
REFERENCE   10 (bases 1 to 2152)
  AUTHORS   Kjeldsen,L., Cowland,J.B., Johnsen,A.H. and Borregaard,N.
  TITLE     SGP28, a novel matrix glycoprotein in specific granules of human
            neutrophils with similarity to a human testis-specific gene product
            and a rodent sperm-coating glycoprotein
  JOURNAL   FEBS Lett 380 (3), 246-250 (1996)
   PUBMED   8601434
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB210604.1, X95240.1 and
            AL121950.8.
            
            On Jun 2, 2019 this sequence version replaced NM_006061.3.
            
            Summary: This gene encodes a member of the cysteine-rich secretory
            protein (CRISP) family within the CRISP, antigen 5 and
            pathogenesis-related 1 proteins superfamily. The encoded protein
            has an N-terminal CRISP, antigen 5 and pathogenesis-related 1
            proteins domain, a hinge region, and a C-terminal ion channel
            regulator domain. This protein contains cysteine residues, located
            in both the N- and C-terminal domains, that form eight disulfide
            bonds, a distinguishing characteristic of this family. This gene is
            expressed in the male reproductive tract where it plays a role in
            sperm function and fertilization, and the female reproductive tract
            where it plays a role in endometrial receptivity for embryo
            implantation. This gene is upregulated in certain types of prostate
            cancer. Alternative splicing results in multiple transcript
            variants. [provided by RefSeq, Nov 2016].
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            CCDS Note: The coding region has been updated to extend the
            N-terminus to one that is more supported by the available
            transcript data. The updated start codon is found only in primates
            and it has a weak Kozak signal. It is possible that ribosomal leaky
            scanning may sometimes allow the better conserved downstream start
            codon to be used. There is no experimental evidence to indicate
            which start codon is preferentially used in vivo.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X95240.1, X94323.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2151887 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000263045.9/ ENSP00000263045.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-45                DB210604.1         68-112
            46-2008             X95240.1           1-1963
            2009-2009           AL121950.8         116401-116401       c
            2010-2152           X95240.1           1965-2107
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p12.3"
     gene            1..2152
                     /gene="CRISP3"
                     /gene_synonym="Aeg2; CRISP-3; CRS3; dJ442L6.3; SGP28"
                     /note="cysteine rich secretory protein 3"
                     /db_xref="GeneID:10321"
                     /db_xref="HGNC:HGNC:16904"
                     /db_xref="MIM:618062"
     exon            1..58
                     /gene="CRISP3"
                     /gene_synonym="Aeg2; CRISP-3; CRS3; dJ442L6.3; SGP28"
                     /inference="alignment:Splign:2.1.0"
     CDS             22..798
                     /gene="CRISP3"
                     /gene_synonym="Aeg2; CRISP-3; CRS3; dJ442L6.3; SGP28"
                     /note="isoform 1 is encoded by transcript variant 1;
                     specific granule protein (28 kDa)"
                     /codon_start=1
                     /product="cysteine-rich secretory protein 3 isoform 1"
                     /protein_id="NP_006052.2"
                     /db_xref="CCDS:CCDS4929.2"
                     /db_xref="GeneID:10321"
                     /db_xref="HGNC:HGNC:16904"
                     /db_xref="MIM:618062"
                     /translation="MKQILHPALETTAMTLFPVLLFLVAGLLPSFPANEDKDPAFTAL
                     LTTQTQVQREIVNKHNELRRAVSPPARNMLKMEWNKEAAANAQKWANQCNYRHSNPKD
                     RMTSLKCGENLYMSSASSSWSQAIQSWFDEYNDFDFGVGPKTPNAVVGHYTQVVWYSS
                     YLVGCGNAYCPNQKVLKYYYVCQYCPAGNWANRLYVPYEQGAPCASCPDNCDDGLCTN
                     GCKYEDLYSNCKSLKLTLTCKHQLVRDSCKASCNCSNSIY"
     exon            59..132
                     /gene="CRISP3"
                     /gene_synonym="Aeg2; CRISP-3; CRS3; dJ442L6.3; SGP28"
                     /inference="alignment:Splign:2.1.0"
     exon            133..249
                     /gene="CRISP3"
                     /gene_synonym="Aeg2; CRISP-3; CRS3; dJ442L6.3; SGP28"
                     /inference="alignment:Splign:2.1.0"
     exon            250..337
                     /gene="CRISP3"
                     /gene_synonym="Aeg2; CRISP-3; CRS3; dJ442L6.3; SGP28"
                     /inference="alignment:Splign:2.1.0"
     exon            338..483
                     /gene="CRISP3"
                     /gene_synonym="Aeg2; CRISP-3; CRS3; dJ442L6.3; SGP28"
                     /inference="alignment:Splign:2.1.0"
     exon            484..581
                     /gene="CRISP3"
                     /gene_synonym="Aeg2; CRISP-3; CRS3; dJ442L6.3; SGP28"
                     /inference="alignment:Splign:2.1.0"
     exon            582..670
                     /gene="CRISP3"
                     /gene_synonym="Aeg2; CRISP-3; CRS3; dJ442L6.3; SGP28"
                     /inference="alignment:Splign:2.1.0"
     exon            671..2152
                     /gene="CRISP3"
                     /gene_synonym="Aeg2; CRISP-3; CRS3; dJ442L6.3; SGP28"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcaccttcct tctgtcaata gatgaaacaa atacttcatc ctgctctgga aaccactgca
       61 atgacattat tcccagtgct gttgttcctg gttgctgggc tgcttccatc ttttccagca
      121 aatgaagata aggatcccgc ttttactgct ttgttaacca cccaaacaca agtgcaaagg
      181 gagattgtga ataagcacaa tgaactgagg agagcagtat ctccccctgc cagaaacatg
      241 ctgaagatgg aatggaacaa agaggctgca gcaaatgccc aaaagtgggc aaaccagtgc
      301 aattacagac acagtaaccc aaaggatcga atgacaagtc taaaatgtgg tgagaatctc
      361 tacatgtcaa gtgcctccag ctcatggtca caagcaatcc aaagctggtt tgatgagtac
      421 aatgattttg actttggtgt agggccaaag actcccaacg cagtggttgg acattataca
      481 caggttgttt ggtactcttc atacctcgtt ggatgtggaa atgcctactg tcccaatcaa
      541 aaagttctaa aatactacta tgtttgccaa tattgtcctg ctggtaattg ggctaataga
      601 ctatatgtcc cttatgaaca aggagcacct tgtgccagtt gcccagataa ctgtgacgat
      661 ggactatgca ccaatggttg caagtacgaa gatctctata gtaactgtaa aagtttgaag
      721 ctcacattaa cctgtaaaca tcagttggtc agggacagtt gcaaggcctc ctgcaattgt
      781 tcaaacagca tttattaaat acgcattaca caccgagtag ggctatgtag agaggagtca
      841 gattatctac ttagatttgg catctactta gatttaacat atactagctg agaaattgta
      901 ggcatgtttg atacacattt gatttcaaat gtttttcttc tggatctgct ttttatttta
      961 caaaaatatt tttcatacaa atggttaaaa agaaacaaaa tctataacaa caactttgga
     1021 tttttatata taaactttgt gatttaaatt tactgaattt aattagggtg aaaattttga
     1081 aagttgtatt ctcatatgac taagttcact aaaaccctgg attgaaagtg aaaattatgt
     1141 tcctagaaca aaatgtacaa aaagaacaat ataattttca catgaaccct tggctgtagt
     1201 tgcctttcct agctccactc taaggctaag catcttcaaa gacgttttcc catatgctgt
     1261 cttaattctt ttcactcatt cacccttctt cccaatcatc tggctggcat cctcacaatt
     1321 gagttgaagc tgttcctcct aaaacaatcc tgacttttat tttgccaaaa tcaatacaat
     1381 cctttgaatt ttttatctgc ataaatttta cagtagaata tgatcaaacc ttcattttta
     1441 aacctctctt ctctttgaca aaacttcctt aaaaaagaat acaagataat ataggtaaat
     1501 accctccact caaggaggta gaactcagtc ctctcccttg tgagtcttca ctaaaatcag
     1561 tgactcactt ccaaagagtg gagtatggaa agggaaacat agtaacttta caggggagaa
     1621 aaatgacaaa tgacgtcttc accaagtgat caaaattaac gtcaccagtg ataagtcatt
     1681 cagatttgtt ctagataatc tttctaaaaa ttcataatcc caatctaatt atgagctaaa
     1741 acatccagca aactcaagtt gaaggacatt ctacaaaata tccctggggt attttagagt
     1801 attcctcaaa actgtaaaaa tcatggaaaa taagggaatc ctgagaaaca atcacagacc
     1861 acatgagact aaggagacat gtgagccaaa tgcaatgtgc ttcttggatc agatcctgga
     1921 acagaaaaag atcagtaatg aaaaaactga tgaagtctga atagaatctg gagtattttt
     1981 aacagtagtg ttgatttctt aatcttgata aatatagcag ggtaatgtaa gatgataacg
     2041 ttagagaaac tgaaactggg tgagggctat ctaggaattc tctgtactat cttaccaaat
     2101 tttcggtaag tctaagaaag caatgcaaaa taaaaagtgt cttgaaaaaa aa
//
