LOCUS       NM_003296               1365 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens cysteine rich secretory protein 2 (CRISP2), transcript
            variant 1, mRNA.
ACCESSION   NM_003296
VERSION     NM_003296.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Lecamwasam,A., Novakovic,B., Meyer,B., Ekinci,E.I., Dwyer,K.M. and
            Saffery,R.
  TITLE     DNA methylation profiling identifies epigenetic differences between
            early versus late stages of diabetic chronic kidney disease
  JOURNAL   Nephrol Dial Transplant 36 (11), 2027-2038 (2021)
   PUBMED   33146725
  REMARK    GeneRIF: DNA methylation profiling identifies epigenetic
            differences between early versus late stages of diabetic chronic
            kidney disease.
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Manfrevola,F., Ferraro,B., Sellitto,C., Rocco,D., Fasano,S.,
            Pierantoni,R. and Chianese,R.
  TITLE     CRISP2, CATSPER1 and PATE1 Expression in Human Asthenozoospermic
            Semen
  JOURNAL   Cells 10 (8), 1956 (2021)
   PUBMED   34440724
  REMARK    GeneRIF: CRISP2, CATSPER1 and PATE1 Expression in Human
            Asthenozoospermic Semen.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1365)
  AUTHORS   Li,Z., Chen,J., Zhao,S., Li,Y., Zhou,J., Liang,J. and Tang,H.
  TITLE     Discovery and validation of novel biomarkers for detection of
            cervical cancer
  JOURNAL   Cancer Med 10 (6), 2063-2074 (2021)
   PUBMED   33624385
  REMARK    GeneRIF: Discovery and validation of novel biomarkers for detection
            of cervical cancer.
REFERENCE   4  (bases 1 to 1365)
  AUTHORS   Zhou,J.H., Zhou,Q.Z., Yang,J.K., Lyu,X.M., Bian,J., Guo,W.B.,
            Chen,Z.J., Xia,M., Xia,H., Qi,T., Li,X. and Liu,C.D.
  TITLE     MicroRNA-27a-mediated repression of cysteine-rich secretory protein
            2 translation in asthenoteratozoospermic patients
  JOURNAL   Asian J Androl 19 (5), 591-595 (2017)
   PUBMED   27517483
  REMARK    GeneRIF: low expression of CRISP2 protein rather than its mRNA was
            observed in the ejaculated spermatozoa from asthenoteratozoospermic
            patients as compared with normozoospermic males
            Erratum:[Asian J Androl. 2023 Nov 1;25(6):758. doi:
            10.4103/aja202348.. PMID: 37750804]
REFERENCE   5  (bases 1 to 1365)
  AUTHORS   Istas,G., Declerck,K., Pudenz,M., Szic,K.S.V.,
            Lendinez-Tortajada,V., Leon-Latre,M., Heyninck,K., Haegeman,G.,
            Casasnovas,J.A., Tellez-Plaza,M., Gerhauser,C., Heiss,C.,
            Rodriguez-Mateos,A. and Berghe,W.V.
  TITLE     Identification of differentially methylated BRCA1 and CRISP2 DNA
            regions as blood surrogate markers for cardiovascular disease
  JOURNAL   Sci Rep 7 (1), 5120 (2017)
   PUBMED   28698603
  REMARK    GeneRIF: The integrated network analysis identified BRCA1 and
            CRISP2 differentially methylated regions (DMR) as the most central
            disease-associated DNA methylation biomarkers. Moreover,
            methylation changes at BRCA1 and CRISP2 specific CpG sites were
            consistently associated with subclinical atherosclerosis measures.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1365)
  AUTHORS   Jamsai,D., Reilly,A., Smith,S.J., Gibbs,G.M., Baker,H.W.,
            McLachlan,R.I., de Kretser,D.M. and O'Bryan,M.K.
  TITLE     Polymorphisms in the human cysteine-rich secretory protein 2
            (CRISP2) gene in Australian men
  JOURNAL   Hum Reprod 23 (9), 2151-2159 (2008)
   PUBMED   18550510
  REMARK    GeneRIF: Polymorphisms within the CRISP2 gene have no significant
            association with male infertility.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 1365)
  AUTHORS   Du,Y., Huang,X., Li,J., Hu,Y., Zhou,Z. and Sha,J.
  TITLE     Human testis specific protein 1 expression in human spermatogenesis
            and involvement in the pathogenesis of male infertility
  JOURNAL   Fertil Steril 85 (6), 1852-1854 (2006)
   PUBMED   16759931
  REMARK    GeneRIF: The protein level and localization of TPX1 were altered in
            patients with spermatogenic arrest and in infertile men with
            oligoasthenoteratospermia syndrome.
REFERENCE   8  (bases 1 to 1365)
  AUTHORS   Busso,D., Cohen,D.J., Hayashi,M., Kasahara,M. and Cuasnicu,P.S.
  TITLE     Human testicular protein TPX1/CRISP-2: localization in spermatozoa,
            fate after capacitation and relevance for gamete interaction
  JOURNAL   Mol Hum Reprod 11 (4), 299-305 (2005)
   PUBMED   15734896
  REMARK    GeneRIF: TPX1 is a component of the sperm acrosome that remains
            associated with sperm after capacitation and acrosome reaction ,
            and is relevant for sperm-oocyte interaction.
REFERENCE   9  (bases 1 to 1365)
  AUTHORS   Kratzschmar,J., Haendler,B., Eberspaecher,U., Roosterman,D.,
            Donner,P. and Schleuning,W.D.
  TITLE     The human cysteine-rich secretory protein (CRISP) family. Primary
            structure and tissue distribution of CRISP-1, CRISP-2 and CRISP-3
  JOURNAL   Eur J Biochem 236 (3), 827-836 (1996)
   PUBMED   8665901
REFERENCE   10 (bases 1 to 1365)
  AUTHORS   Kasahara,M., Gutknecht,J., Brew,K., Spurr,N. and Goodfellow,P.N.
  TITLE     Cloning and mapping of a testis-specific gene with sequence
            similarity to a sperm-coating glycoprotein gene
  JOURNAL   Genomics 5 (3), 527-534 (1989)
   PUBMED   2613236
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AY773479.1 and DB524518.1.
            
            On Nov 23, 2018 this sequence version replaced NM_003296.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript. All variants encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X95239.1, SRR5189667.325060.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1968832,
                                           SAMEA1968968 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000339139.5/ ENSP00000339155.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1358              AY773479.1         28-1385
            1359-1365           DB524518.1         472-478
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p12.3"
     gene            1..1365
                     /gene="CRISP2"
                     /gene_synonym="CRISP-2; CT36; GAPDL5; TPX1; TSP1"
                     /note="cysteine rich secretory protein 2"
                     /db_xref="GeneID:7180"
                     /db_xref="HGNC:HGNC:12024"
                     /db_xref="MIM:187430"
     exon            1..69
                     /gene="CRISP2"
                     /gene_synonym="CRISP-2; CT36; GAPDL5; TPX1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            70..173
                     /gene="CRISP2"
                     /gene_synonym="CRISP-2; CT36; GAPDL5; TPX1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            174..210
                     /gene="CRISP2"
                     /gene_synonym="CRISP-2; CT36; GAPDL5; TPX1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            211..285
                     /gene="CRISP2"
                     /gene_synonym="CRISP-2; CT36; GAPDL5; TPX1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             220..951
                     /gene="CRISP2"
                     /gene_synonym="CRISP-2; CT36; GAPDL5; TPX1; TSP1"
                     /note="glyceraldehyde-3-phosphate dehydrogenase-like 5;
                     testis specific protein 1 (probe H4-1 p3-1); cancer/testis
                     antigen 36; testis-specific protein TPX-1; testicular
                     tissue protein Li 43"
                     /codon_start=1
                     /product="cysteine-rich secretory protein 2 precursor"
                     /protein_id="NP_003287.1"
                     /db_xref="CCDS:CCDS4928.1"
                     /db_xref="GeneID:7180"
                     /db_xref="HGNC:HGNC:12024"
                     /db_xref="MIM:187430"
                     /translation="MALLPVLFLVTVLLPSLPAEGKDPAFTALLTTQLQVQREIVNKH
                     NELRKAVSPPASNMLKMEWSREVTTNAQRWANKCTLQHSDPEDRKTSTRCGENLYMSS
                     DPTSWSSAIQSWYDEILDFVYGVGPKSPNAVVGHYTQLVWYSTYQVGCGIAYCPNQDS
                     LKYYYVCQYCPAGNNMNRKNTPYQQGTPCAGCPDDCDKGLCTNSCQYQDLLSNCDSLK
                     NTAGCEHELLKEKCKATCLCENKIY"
     exon            286..402
                     /gene="CRISP2"
                     /gene_synonym="CRISP-2; CT36; GAPDL5; TPX1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            403..490
                     /gene="CRISP2"
                     /gene_synonym="CRISP-2; CT36; GAPDL5; TPX1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            491..636
                     /gene="CRISP2"
                     /gene_synonym="CRISP-2; CT36; GAPDL5; TPX1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            637..734
                     /gene="CRISP2"
                     /gene_synonym="CRISP-2; CT36; GAPDL5; TPX1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            735..823
                     /gene="CRISP2"
                     /gene_synonym="CRISP-2; CT36; GAPDL5; TPX1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            824..1365
                     /gene="CRISP2"
                     /gene_synonym="CRISP-2; CT36; GAPDL5; TPX1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1341..1346
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CRISP2"
                     /gene_synonym="CRISP-2; CT36; GAPDL5; TPX1; TSP1"
                     /note="hexamer: AATAAA"
     polyA_site      1365
                     /gene="CRISP2"
                     /gene_synonym="CRISP-2; CT36; GAPDL5; TPX1; TSP1"
                     /note="major polyA site"
ORIGIN      
        1 gcagctcctc aacgccgcaa cgcgccggcc caactgcagg aaggtctgtg ctctggagcc
       61 agggtaaatg gttataaaat tatacaccat ggccctccta aagacactct aggaaaacca
      121 tgtcatcctg atcttaaaac acctgcaaga aagagcacag tacttcacca ttaataaagt
      181 agatatttca tcctgctcag aaaaccaaca tttccagcaa tggctttact accggtgttg
      241 tttctggtta ctgtgctgct tccatcttta cctgcagaag gaaaggatcc cgcttttact
      301 gctttgttaa ccacccagtt gcaagtgcaa agggagattg taaataaaca caatgaacta
      361 aggaaagcag tctctccacc tgccagtaac atgctaaaga tggaatggag cagagaggta
      421 acaacgaatg cccaaaggtg ggcaaacaag tgcactttac aacatagtga tccagaggac
      481 cgcaaaacca gtacaagatg tggtgagaat ctctatatgt caagtgaccc tacttcctgg
      541 tcttctgcaa tccaaagctg gtatgacgag atcctagatt ttgtctatgg tgtaggacca
      601 aagagtccca atgcagttgt tggacattat actcagcttg tttggtactc gacttaccag
      661 gtaggctgtg gaattgccta ctgtcccaat caagatagtc taaaatacta ctatgtttgc
      721 caatattgtc ctgctggtaa taatatgaat agaaagaata ccccgtacca acaaggaaca
      781 ccttgtgccg gttgccctga tgactgtgac aaaggactat gcaccaatag ttgccagtat
      841 caagatctcc taagtaactg tgattccttg aagaatacag ctggctgtga acatgagtta
      901 ctcaaggaaa agtgcaaggc tacttgccta tgtgagaaca aaatttactg atttacctag
      961 tgagcattgt gcaagactgc atggataagg gctgcatcat ttaattgcga cataccagtg
     1021 gaaattgtat gtatgttagt gacaaatttg atttcaaaga gcaatgcatc ttctccccca
     1081 gatcatcaca gaaatcactt tcaggcaatg atttacaaaa gtagcatagt agatgatgac
     1141 aactgtgaac tctgacataa atttagtgct ttataacgaa ctgaatcagg ttgaggattt
     1201 tgaaaactgt ataaccatag gatttaggtc actaggactt tggatcaaaa tggtgcatta
     1261 cgtatttcct gaaacatgct aaagaagaag actgtaacat cattgccatt cctactacct
     1321 gagtttttac ttgcataaac aataaattca aagctttaca tctgc
//
