LOCUS       NM_001131               1918 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens cysteine rich secretory protein 1 (CRISP1), transcript
            variant 1, mRNA.
ACCESSION   NM_001131
VERSION     NM_001131.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Sheng,J., Gadella,B.M., Olrichs,N.K., Kaloyanova,D.V. and
            Helms,J.B.
  TITLE     The less conserved metal-binding site in human CRISP1 remains
            sensitive to zinc ions to permit protein oligomerization
  JOURNAL   Sci Rep 11 (1), 5498 (2021)
   PUBMED   33750840
  REMARK    GeneRIF: The less conserved metal-binding site in human CRISP1
            remains sensitive to zinc ions to permit protein oligomerization.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Maldera,J.A., Weigel Munoz,M., Chirinos,M., Busso,D., G E Raffo,F.,
            Battistone,M.A., Blaquier,J.A., Larrea,F. and Cuasnicu,P.S.
  TITLE     Human fertilization: epididymal hCRISP1 mediates sperm-zona
            pellucida binding through its interaction with ZP3
  JOURNAL   Mol Hum Reprod 20 (4), 341-349 (2014)
   PUBMED   24334245
  REMARK    GeneRIF: Epididymal CRISP1 mediates sperm-zona pellucida binding
            through its interaction with ZP3.
REFERENCE   3  (bases 1 to 1918)
  AUTHORS   Legare,C., Cloutier,F., Makosso-Kallyth,S., Laflamme,N., Jarvi,K.,
            Tremblay,R.R. and Sullivan,R.
  TITLE     Cysteine-rich secretory protein 1 in seminal plasma: potential
            biomarker for the distinction between obstructive and
            nonobstructive azoospermia
  JOURNAL   Fertil Steril 100 (5), 1253-1260 (2013)
   PUBMED   23987519
  REMARK    GeneRIF: Seminal CRISP1 combined with neutral alpha glucosidase
            activity can potentially distinguish between obstructive and
            nonobstructive azoospermia.
REFERENCE   4  (bases 1 to 1918)
  AUTHORS   de Mateo,S., Castillo,J., Estanyol,J.M., Ballesca,J.L. and Oliva,R.
  TITLE     Proteomic characterization of the human sperm nucleus
  JOURNAL   Proteomics 11 (13), 2714-2726 (2011)
   PUBMED   21630459
REFERENCE   5  (bases 1 to 1918)
  AUTHORS   Legare,C., Boudreau,L., Thimon,V., Thabet,M. and Sullivan,R.
  TITLE     Vasectomy affects cysteine-rich secretory protein expression along
            the human epididymis and its association with ejaculated
            spermatozoa following vasectomy surgical reversal
  JOURNAL   J Androl 31 (6), 573-583 (2010)
   PUBMED   20378925
  REMARK    GeneRIF: CRISP1 was in the seminal plasma of normal and
            vasovasostomized men, but not in that of vasectomized men. The
            soluble concentration of CRISP1 was significantly higher in the
            seminal plasma of vasovasostomized men as compared with that from
            normal men.
REFERENCE   6  (bases 1 to 1918)
  AUTHORS   Cohen,D.J., Ellerman,D.A., Busso,D., Morgenfeld,M.M., Piazza,A.D.,
            Hayashi,M., Young,E.T., Kasahara,M. and Cuasnicu,P.S.
  TITLE     Evidence that human epididymal protein ARP plays a role in gamete
            fusion through complementary sites on the surface of the human egg
  JOURNAL   Biol Reprod 65 (4), 1000-1005 (2001)
   PUBMED   11566719
REFERENCE   7  (bases 1 to 1918)
  AUTHORS   Kirchhoff,C.
  TITLE     Molecular characterization of epididymal proteins
  JOURNAL   Rev Reprod 3 (2), 86-95 (1998)
   PUBMED   9685187
  REMARK    Review article
REFERENCE   8  (bases 1 to 1918)
  AUTHORS   Hayashi,M., Fujimoto,S., Takano,H., Ushiki,T., Abe,K., Ishikura,H.,
            Yoshida,M.C., Kirchhoff,C., Ishibashi,T. and Kasahara,M.
  TITLE     Characterization of a human glycoprotein with a potential role in
            sperm-egg fusion: cDNA cloning, immunohistochemical localization,
            and chromosomal assignment of the gene (AEGL1)
  JOURNAL   Genomics 32 (3), 367-374 (1996)
   PUBMED   8838800
REFERENCE   9  (bases 1 to 1918)
  AUTHORS   Kratzschmar,J., Haendler,B., Eberspaecher,U., Roosterman,D.,
            Donner,P. and Schleuning,W.D.
  TITLE     The human cysteine-rich secretory protein (CRISP) family. Primary
            structure and tissue distribution of CRISP-1, CRISP-2 and CRISP-3
  JOURNAL   Eur J Biochem 236 (3), 827-836 (1996)
   PUBMED   8665901
REFERENCE   10 (bases 1 to 1918)
  AUTHORS   Hayashi,M.
  TITLE     [Analysis of the human acidic epididymal glycoprotein-like
            molecule: isolation of cDNA and tissue localization]
  JOURNAL   Hokkaido Igaku Zasshi 70 (5), 743-753 (1995)
   PUBMED   8543280
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL359458.17.
            
            On May 17, 2019 this sequence version replaced NM_001131.2.
            
            Summary: Fertilization consists of a sequence of specific cell-cell
            interactions culminating in the fusion of the sperm and egg plasma
            membranes. Recognition, binding, and fusion occur through the
            interaction of complementary molecules that are localized to
            specific domains of the sperm and egg plasma membranes. In the
            sperm, the postacrosomal region or equatorial segment is involved
            in sperm-egg plasma membrane fusion. The protein encoded by this
            gene is a member of the cysteine-rich secretory protein (CRISP)
            family. It is expressed in the epididymis, is secreted into the
            epididymal lumen, and binds to the postacrosomal region of the
            sperm head, where it plays a role in sperm-egg fusion.
            Alternatively spliced transcript variants encoding different
            isoforms have been found for this gene. [provided by RefSeq, Mar
            2011].
            
            Transcript Variant: This variant (1) represents the predominant
            transcript and encodes the longer isoform (1). Variants 1 and 3
            encode the same isoform.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: GQ472203.1, X95237.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1968832,
                                           SAMEA1968968 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000335847.9/ ENSP00000338276.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-107               AL359458.17        46086-46192         c
            108-175             AL359458.17        36992-37059         c
            176-304             AL359458.17        31658-31786         c
            305-395             AL359458.17        27866-27956         c
            396-544             AL359458.17        26177-26325         c
            545-642             AL359458.17        20555-20652         c
            643-731             AL359458.17        18094-18182         c
            732-1918            AL359458.17        13914-15100         c
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p12.3"
     gene            1..1918
                     /gene="CRISP1"
                     /gene_synonym="AEGL1; ARP; CRISP-1; HEL-S-57; HSCRISP1D;
                     HSCRISP1G; HUMARP"
                     /note="cysteine rich secretory protein 1"
                     /db_xref="GeneID:167"
                     /db_xref="HGNC:HGNC:304"
                     /db_xref="MIM:601193"
     exon            1..107
                     /gene="CRISP1"
                     /gene_synonym="AEGL1; ARP; CRISP-1; HEL-S-57; HSCRISP1D;
                     HSCRISP1G; HUMARP"
                     /inference="alignment:Splign:2.1.0"
     exon            108..175
                     /gene="CRISP1"
                     /gene_synonym="AEGL1; ARP; CRISP-1; HEL-S-57; HSCRISP1D;
                     HSCRISP1G; HUMARP"
                     /inference="alignment:Splign:2.1.0"
     CDS             110..859
                     /gene="CRISP1"
                     /gene_synonym="AEGL1; ARP; CRISP-1; HEL-S-57; HSCRISP1D;
                     HSCRISP1G; HUMARP"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; acidic epididymal glycoprotein-like 1;
                     cysteine-rich secretory protein-1 delta; AEG-related
                     protein; AEG-like protein; acidic epididymal glycoprotein
                     homolog; epididymis secretory protein Li 57"
                     /codon_start=1
                     /product="cysteine-rich secretory protein 1 isoform 1
                     precursor"
                     /protein_id="NP_001122.2"
                     /db_xref="CCDS:CCDS4931.1"
                     /db_xref="GeneID:167"
                     /db_xref="HGNC:HGNC:304"
                     /db_xref="MIM:601193"
                     /translation="MEIKHLLFLVAAACLLPMLSMKKKSARDQFNKLVTDLPNVQEEI
                     VNIHNALRRRVVPPASNMLKMSWSEEAAQNARIFSKYCDMTESNPLERRLPNTFCGEN
                     MHMTSYPVSWSSVIGVWYSESTSFKHGEWTTTDDDITTDHYTQIVWATSYLIGCAIAS
                     CRQQGSPRYLYVCHYCHEGNDPETKNEPYKTGVPCEACPSNCEDKLCTNPCIYYDEYF
                     DCDIQVHYLGCNHSTTILFCKATCLCDTEIK"
     exon            176..304
                     /gene="CRISP1"
                     /gene_synonym="AEGL1; ARP; CRISP-1; HEL-S-57; HSCRISP1D;
                     HSCRISP1G; HUMARP"
                     /inference="alignment:Splign:2.1.0"
     exon            305..395
                     /gene="CRISP1"
                     /gene_synonym="AEGL1; ARP; CRISP-1; HEL-S-57; HSCRISP1D;
                     HSCRISP1G; HUMARP"
                     /inference="alignment:Splign:2.1.0"
     exon            396..544
                     /gene="CRISP1"
                     /gene_synonym="AEGL1; ARP; CRISP-1; HEL-S-57; HSCRISP1D;
                     HSCRISP1G; HUMARP"
                     /inference="alignment:Splign:2.1.0"
     exon            545..642
                     /gene="CRISP1"
                     /gene_synonym="AEGL1; ARP; CRISP-1; HEL-S-57; HSCRISP1D;
                     HSCRISP1G; HUMARP"
                     /inference="alignment:Splign:2.1.0"
     exon            643..731
                     /gene="CRISP1"
                     /gene_synonym="AEGL1; ARP; CRISP-1; HEL-S-57; HSCRISP1D;
                     HSCRISP1G; HUMARP"
                     /inference="alignment:Splign:2.1.0"
     exon            732..1918
                     /gene="CRISP1"
                     /gene_synonym="AEGL1; ARP; CRISP-1; HEL-S-57; HSCRISP1D;
                     HSCRISP1G; HUMARP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1293..1298
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CRISP1"
                     /gene_synonym="AEGL1; ARP; CRISP-1; HEL-S-57; HSCRISP1D;
                     HSCRISP1G; HUMARP"
                     /note="hexamer: ATTAAA"
     polyA_site      1315
                     /gene="CRISP1"
                     /gene_synonym="AEGL1; ARP; CRISP-1; HEL-S-57; HSCRISP1D;
                     HSCRISP1G; HUMARP"
                     /note="major polyA site"
ORIGIN      
        1 attgaacaaa tctcccctgt ggactgtcat ttaaaagcac aaatacacta catagagaaa
       61 ggcttggttc ttatcaggac acaaatttaa aggctgtgtg gacttgggga tggaaattaa
      121 acacctcttg tttttggttg ctgctgcttg cttactgcct atgttgtcca tgaaaaagaa
      181 atcagctaga gaccaattta ataagctcgt caccgacttg ccaaatgtac aagaagagat
      241 cgttaatata cacaacgccc tcaggagaag agtagttcca ccagccagca acatgctgaa
      301 gatgagttgg agtgaagagg ctgcacaaaa tgccagaatt ttttcaaagt attgtgatat
      361 gacagagagc aacccccttg agaggagact tccaaatacc ttttgtggag aaaatatgca
      421 tatgacatct tatcctgtat catggtcaag tgtaattgga gtctggtaca gtgagtctac
      481 aagtttcaaa catggagaat ggacaacaac ggatgatgac ataactactg accactacac
      541 tcagattgtt tgggccacat cttacctgat tggctgtgcc attgcatctt gccgccaaca
      601 aggatcacct cgatatctct acgtttgtca ctattgtcat gagggaaatg atcctgaaac
      661 aaagaatgaa ccttataaga caggcgtccc atgtgaagcc tgcccaagta actgtgaaga
      721 caaactttgc actaacccct gcatctacta tgatgaatac ttcgactgtg acatacaagt
      781 ccattatctg ggatgcaacc actcaacaac tatcctattc tgtaaagcca cttgtctgtg
      841 tgacactgag ataaaatagg tctttgttat tttcaactgt tctatgctgt gacgatgagg
      901 aggagatgtc tgttggattc atgtcttttg ctatagttca gtagcttctg ctaaatttca
      961 ctgattttaa tcatgctgga gaccttaact cccatcctga tacatcctga agtaacactg
     1021 ttttaaactt tcttagtgct ggagtaaaag gtcaagtcca acacctgcct taaatttaaa
     1081 tcatgtgatt tatagttttt aagttggcat aattcaactt atggtataac tgggtccctc
     1141 aacagtaacc tgggctaaaa taggtcttat gtggttcaac tcccaccccc gccttcccca
     1201 tattttcaac cactctgatt atcttccctg cacaactaac atccagtaat aattcttcac
     1261 ttttaaaatt ttacttctac tttaaatcaa tcattaaagg aatccacaaa gcaaacagag
     1321 ttcagtctca tcttgcaagg taaatatcat ttaattggaa gtagtttaaa tgtctcattg
     1381 ttttattgac acatctatat atacatttgt gaagcaagaa acaataaaaa agcttcgtat
     1441 gccattaatt taacaaaata tgtattcagt actgattgca tacaagatgc atgtttatat
     1501 atatggaagg aatatagttt catttcattg caaaggcagt ataaaagata tataaaatag
     1561 cataatatga gaaattaagt ccctaaagac atataggtca catattatta ttgccagatg
     1621 agcataaata gcttctgttt ggagattcag gaaagcctta gggtggaatg aggaacatct
     1681 tctgagtaaa cagggttgca aaggttatga ttatttcaac acaatggaag agcacagtta
     1741 aggccaacta acgtaaaatg cactgaagcc ttagggaata ttgaagggcc tgacatgggg
     1801 aaagggaagg ctaaaaatac ttggtcaaat tttaacatta taccaaagtt atacccagtt
     1861 ctacctactt gtatatttct ttactcattt caataaagtg tttgaaaaaa aaaaaaaa
//
