LOCUS       NM_139161               1086 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens crumbs cell polarity complex component 3 (CRB3),
            transcript variant 2, mRNA.
ACCESSION   NM_139161
VERSION     NM_139161.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Wang,Z., Wang,X., Liu,L., Guo,X., Zhang,H., Yin,J., Lin,R., Shao,Y.
            and Cai,D.
  TITLE     Fructose-bisphosphatase1 (FBP1) alleviates experimental
            osteoarthritis by regulating Protein crumbs homolog 3 (CRB3)
  JOURNAL   Arthritis Res Ther 25 (1), 235 (2023)
   PUBMED   38049890
  REMARK    GeneRIF: Fructose-bisphosphatase1 (FBP1) alleviates experimental
            osteoarthritis by regulating Protein crumbs homolog 3 (CRB3).
            Erratum:[Arthritis Res Ther. 2023 Dec 28;25(1):250. doi:
            10.1186/s13075-023-03247-9.. PMID: 38155346]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Tilston-Lunel,A., Mazzilli,S., Kingston,N.M., Szymaniak,A.D.,
            Hicks-Berthet,J., Kern,J.G., Abo,K., Reid,M.E., Perdomo,C.,
            Wilson,A.A., Spira,A., Beane,J. and Varelas,X.
  TITLE     Aberrant epithelial polarity cues drive the development of
            precancerous airway lesions
  JOURNAL   Proc Natl Acad Sci U S A 118 (18) (2021)
   PUBMED   33903236
  REMARK    GeneRIF: Aberrant epithelial polarity cues drive the development of
            precancerous airway lesions.
REFERENCE   3  (bases 1 to 1086)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 1086)
  AUTHORS   Iioka,H., Saito,K. and Kondo,E.
  TITLE     Crumbs3 regulates the expression of glycosphingolipids on the
            plasma membrane to promote colon cancer cell migration
  JOURNAL   Biochem Biophys Res Commun 519 (2), 287-293 (2019)
   PUBMED   31500807
  REMARK    GeneRIF: The Crb3 may promote colon cancer cell migration by
            regulating the expression of GSLs on the cell surface.
REFERENCE   5  (bases 1 to 1086)
  AUTHORS   Madigan,V.J., Tyson,T.O., Yuziuk,J.A., Pillai,M., Moller-Tank,S.
            and Asokan,A.
  TITLE     A CRISPR Screen Identifies the Cell Polarity Determinant Crumbs 3
            as an Adeno-associated Virus Restriction Factor in Hepatocytes
  JOURNAL   J Virol 93 (21), e00943-19 (2019)
   PUBMED   31391273
  REMARK    GeneRIF: Knockout (KO) of apical polarity determinant Crumbs 3
            (Crb3) enhances Adeno-associated viruses (AAV) transduction, while
            overexpression exerts the opposite effect. Crb3 restricts AAV
            transduction in a serotype- and cell type-specific manner.
            Increased availability of galactosylated glycans on the surfaces of
            Crb3 KO cells, but not the universal AAV receptor, leads to
            increased capsid attachment and enhanced transduction.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1086)
  AUTHORS   Fogg,V.C., Liu,C.J. and Margolis,B.
  TITLE     Multiple regions of Crumbs3 are required for tight junction
            formation in MCF10A cells
  JOURNAL   J Cell Sci 118 (Pt 13), 2859-2869 (2005)
   PUBMED   15976445
  REMARK    GeneRIF: The crumbs3 protein plays an important role in epithelial
            tight junction formation, and also provide the first known
            functional role for the mammalian Crumbs FERM binding domain.
REFERENCE   7  (bases 1 to 1086)
  AUTHORS   Lemmers,C., Michel,D., Lane-Guermonprez,L., Delgrossi,M.H.,
            Medina,E., Arsanto,J.P. and Le Bivic,A.
  TITLE     CRB3 binds directly to Par6 and regulates the morphogenesis of the
            tight junctions in mammalian epithelial cells
  JOURNAL   Mol Biol Cell 15 (3), 1324-1333 (2004)
   PUBMED   14718572
  REMARK    GeneRIF: CRB3, through its cytoplasmic domain and its interactors,
            plays a role in apical membrane morphogenesis and tight junction
            regulation.
REFERENCE   8  (bases 1 to 1086)
  AUTHORS   Roh,M.H., Fan,S., Liu,C.J. and Margolis,B.
  TITLE     The Crumbs3-Pals1 complex participates in the establishment of
            polarity in mammalian epithelial cells
  JOURNAL   J Cell Sci 116 (Pt 14), 2895-2906 (2003)
   PUBMED   12771187
  REMARK    GeneRIF: the Crumbs3-Pals1 complex has a role in development of
            polarity in mammalian epithelial cells
REFERENCE   9  (bases 1 to 1086)
  AUTHORS   Makarova,O., Roh,M.H., Liu,C.J., Laurinec,S. and Margolis,B.
  TITLE     Mammalian Crumbs3 is a small transmembrane protein linked to
            protein associated with Lin-7 (Pals1)
  JOURNAL   Gene 302 (1-2), 21-29 (2003)
   PUBMED   12527193
REFERENCE   10 (bases 1 to 1086)
  AUTHORS   Lemmers,C., Medina,E., Delgrossi,M.H., Michel,D., Arsanto,J.P. and
            Le Bivic,A.
  TITLE     hINADl/PATJ, a homolog of discs lost, interacts with crumbs and
            localizes to tight junctions in human epithelial cells
  JOURNAL   J Biol Chem 277 (28), 25408-25415 (2002)
   PUBMED   11964389
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB215367.1, AF503290.1 and
            BC002652.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_139161.4.
            
            Summary: This gene encodes a member of the Crumbs family of
            proteins. This gene is widely expressed in epithelial tissues where
            the encoded protein isoforms play various roles such as the control
            of cytokinesis and ciliogenesis or the formation of tight
            junctions. Alternative splicing results in multiple transcript
            variants encoding different isoforms. [provided by RefSeq, Mar
            2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF503290.1, AK292956.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000600229.6/ ENSP00000472010.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 DB215367.1         34-41
            9-1073              AF503290.1         1-1065
            1074-1086           BC002652.2         592-604
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.3"
     gene            1..1086
                     /gene="CRB3"
                     /note="crumbs cell polarity complex component 3"
                     /db_xref="GeneID:92359"
                     /db_xref="HGNC:HGNC:20237"
                     /db_xref="MIM:609737"
     exon            1..80
                     /gene="CRB3"
                     /inference="alignment:Splign:2.1.0"
     exon            81..256
                     /gene="CRB3"
                     /inference="alignment:Splign:2.1.0"
     CDS             175..537
                     /gene="CRB3"
                     /note="isoform a precursor is encoded by transcript
                     variant 2; crumbs family member 3; protein crumbs homolog
                     3; crumbs 3, cell polarity complex component"
                     /codon_start=1
                     /product="protein crumbs homolog 3 isoform a precursor"
                     /protein_id="NP_631900.1"
                     /db_xref="CCDS:CCDS12167.1"
                     /db_xref="GeneID:92359"
                     /db_xref="HGNC:HGNC:20237"
                     /db_xref="MIM:609737"
                     /translation="MANPGLGLLLALGLPFLLARWGRAWGQIQTTSANENSTVLPSST
                     SSSSDGNLRPEAITAIIVVFSLLAALLLAVGLALLVRKLREKRQTEGTYRPSSEEQVG
                     ARVPPTPNLKLPPEERLI"
     exon            257..330
                     /gene="CRB3"
                     /inference="alignment:Splign:2.1.0"
     exon            331..1086
                     /gene="CRB3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1058..1063
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CRB3"
                     /note="hexamer: TATAAA"
     polyA_site      1086
                     /gene="CRB3"
                     /note="major polyA site"
ORIGIN      
        1 acagaacgac cgacggaccg agggttcgag ggagggacac ggaccaggaa cctgagctag
       61 gtcaaagacg cccgggccag gtgccccgtc gcaggtgccc ctggccggag atgcggtagg
      121 aggggcgagc gcgagaagcc ccttcctcgg cgctgccaac ccgccaccca gcccatggcg
      181 aaccccgggc tggggctgct tctggcgctg ggcctgccgt tcctgctggc ccgctggggc
      241 cgagcctggg ggcaaataca gaccacttct gcaaatgaga atagcactgt tttgccttca
      301 tccaccagct ccagctccga tggcaacctg cgtccagaag ccatcactgc tatcatcgtg
      361 gtcttctccc tcttggctgc cttgctcctg gctgtggggc tggcactgtt ggtgcggaag
      421 cttcgggaga agcggcagac ggagggcacc taccggccca gtagcgagga gcaggtgggt
      481 gcccgcgtgc caccgacccc caacctcaag ttgccgccgg aagagcggct catctgaacg
      541 ctggggcctg ctgcagccac caacactgcc caggactgcg ggttgctggc ttgtacaccg
      601 cagctgccac cgagacacca gcctctgatg gctcaggagg acttgtgggg agaggctggg
      661 ggcacccatg tggtgggctc tgtgcagcat gttgcctctg cttggctgtg cctgcagctc
      721 agggtgctgg ggctcgggac ccacccccct gcttgcggaa ccaacttttc tctgtgtgtc
      781 cagcaggccc cacaaccccc tctcctttct ttcagttctc ccatgcagcc gaggcccggg
      841 cccctcagga ctccaaggag acggtgcagg gctgcctgcc catctaggtc ccctctcctg
      901 catctgtctc ccttcattgc tgtgtgacct tggggaaagg cagtgccctc tctgggcagt
      961 cagatccacc cagtgcttaa tagcagggaa gaaggtactt caaagactct gcccctgagg
     1021 tcaagagagg atggggctat tcacttttat atatttatat aaaattagta gtgagatgta
     1081 acaaaa
//
