LOCUS       NM_173689               5613 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens crumbs cell polarity complex component 2 (CRB2),
            transcript variant 1, mRNA.
ACCESSION   NM_173689
VERSION     NM_173689.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Stehle,I.F., Imventarza,J.A., Woerz,F., Hoffmann,F., Boldt,K.,
            Beyer,T., Quinn,P.M. and Ueffing,M.
  TITLE     Human CRB1 and CRB2 form homo- and heteromeric protein complexes in
            the retina
  JOURNAL   Life Sci Alliance 7 (6), e202302440 (2024)
   PUBMED   38570189
  REMARK    GeneRIF: Human CRB1 and CRB2 form homo- and heteromeric protein
            complexes in the retina.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  AUTHORS   Tessier,A., Roux,N., Boutaud,L., Lunel,E., Hakkakian,L.,
            Parisot,M., Garfa-Traore,M., Ichkou,A., Elkhartoufi,N., Bole,C.,
            Nitschke,P., Amiel,J., Martinovic,J., Encha-Razavi,F.,
            Attie-Bitach,T. and Thomas,S.
  TITLE     Bi-allelic variations in CRB2, encoding the crumbs cell polarity
            complex component 2, lead to non-communicating hydrocephalus due to
            atresia of the aqueduct of sylvius and central canal of the medulla
  JOURNAL   Acta Neuropathol Commun 11 (1), 29 (2023)
   PUBMED   36803301
  REMARK    GeneRIF: Bi-allelic variations in CRB2, encoding the crumbs cell
            polarity complex component 2, lead to non-communicating
            hydrocephalus due to atresia of the aqueduct of sylvius and central
            canal of the medulla.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5613)
  AUTHORS   Moller-Kerutt,A., Schonhoff,B., Rellmann,Y., George,B., Braun,D.A.,
            Pavenstadt,H. and Weide,T.
  TITLE     Loss of surface transport is a main cellular pathomechanism of CRB2
            variants causing podocytopathies
  JOURNAL   Life Sci Alliance 6 (3), e202201649 (2022)
   PUBMED   36549870
  REMARK    GeneRIF: Loss of surface transport is a main cellular
            pathomechanism of CRB2 variants causing podocytopathies.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5613)
  AUTHORS   Simaab,A., Krishin,J., Alaradi,S.R., Haider,N., Shah,M., Ullah,A.,
            Abdullah,A., Ahmad,W., Hansen,T. and Basit,S.
  TITLE     Exome Sequencing Revealed a Novel Splice Site Variant in the CRB2
            Gene Underlying Nephrotic Syndrome
  JOURNAL   Medicina (Kaunas) 58 (12), 1784 (2022)
   PUBMED   36556986
  REMARK    GeneRIF: Exome Sequencing Revealed a Novel Splice Site Variant in
            the CRB2 Gene Underlying Nephrotic Syndrome.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5613)
  AUTHORS   Wang,Y., Bao,G., Zhang,M., Xiang,J., Zhou,H., Wahafu,A., Wu,W.,
            Ma,X., Huo,L., Bai,X., Xie,W., Liu,P. and Wang,M.
  TITLE     CRB2 enhances malignancy of glioblastoma via activation of the
            NF-kappaB pathway
  JOURNAL   Exp Cell Res 414 (1), 113077 (2022)
   PUBMED   35219647
  REMARK    GeneRIF: CRB2 enhances malignancy of glioblastoma via activation of
            the NF-kappaB pathway.
REFERENCE   6  (bases 1 to 5613)
  AUTHORS   Gosens,I., Sessa,A., den Hollander,A.I., Letteboer,S.J.,
            Belloni,V., Arends,M.L., Le Bivic,A., Cremers,F.P., Broccoli,V. and
            Roepman,R.
  TITLE     FERM protein EPB41L5 is a novel member of the mammalian CRB-MPP5
            polarity complex
  JOURNAL   Exp Cell Res 313 (19), 3959-3970 (2007)
   PUBMED   17920587
REFERENCE   7  (bases 1 to 5613)
  AUTHORS   Roni,V., Carpio,R. and Wissinger,B.
  TITLE     Mapping of transcription start sites of human retina expressed
            genes
  JOURNAL   BMC Genomics 8, 42 (2007)
   PUBMED   17286855
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 5613)
  AUTHORS   Botuyan,M.V., Lee,J., Ward,I.M., Kim,J.E., Thompson,J.R., Chen,J.
            and Mer,G.
  TITLE     Structural basis for the methylation state-specific recognition of
            histone H4-K20 by 53BP1 and Crb2 in DNA repair
  JOURNAL   Cell 127 (7), 1361-1373 (2006)
   PUBMED   17190600
  REMARK    GeneRIF: Using X-ray crystallography and NMR spectroscopy, we show
            that, despite low amino acid sequence conservation, both 53BP1 and
            Crb2 contain tandem tudor domains that interact with histone H4
            specifically dimethylated at Lys20 (H4-K20me2).
REFERENCE   9  (bases 1 to 5613)
  AUTHORS   van den Hurk,J.A., Rashbass,P., Roepman,R., Davis,J.,
            Voesenek,K.E., Arends,M.L., Zonneveld,M.N., van Roekel,M.H.,
            Cameron,K., Rohrschneider,K., Heckenlively,J.R., Koenekoop,R.K.,
            Hoyng,C.B., Cremers,F.P. and den Hollander,A.I.
  TITLE     Characterization of the Crumbs homolog 2 (CRB2) gene and analysis
            of its role in retinitis pigmentosa and Leber congenital amaurosis
  JOURNAL   Mol Vis 11, 263-273 (2005)
   PUBMED   15851977
  REMARK    GeneRIF: This study shows that CRB2 sequence variants are not a
            common cause of autosomal recessive RP and LCA.
            Publication Status: Online-Only
REFERENCE   10 (bases 1 to 5613)
  AUTHORS   Katoh,M. and Katoh,M.
  TITLE     Identification and characterization of Crumbs homolog 2 gene at
            human chromosome 9q33.3
  JOURNAL   Int J Oncol 24 (3), 743-749 (2004)
   PUBMED   14767562
  REMARK    GeneRIF: Crumbs homolog 2 gene maps to human chromosome 9q33.3
            GeneRIF: The CRB2 gene encodes a transmembrane protein (1285 aa)
            and a secreted protein (1176 aa). The transmembrane isoform
            consists of 14 extracellular EGF-like domains, 3 extracellular
            laminin G-like domains, and a Crb cytoplasmic tail domain.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA240339.1, AL365504.33,
            AY720432.1, AL445489.10 and AK124819.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_173689.6.
            
            Summary: This gene encodes a member of a family of proteins that
            are components of the Crumbs cell polarity complex. In mammals,
            members of this family are thought to play a role in many cellular
            processes in early embryonic development. A similar protein in
            Drosophila determines apicobasal polarity in embryonic epithelial
            cells. Mutations in this gene are associated with focal segmental
            glomerulosclerosis 9, and with ventriculomegaly with cystic kidney
            disease. [provided by RefSeq, Aug 2016].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the supported protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY720432.1, SRR14372080.4666007.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1970526, SAMEA2154665
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000373631.8/ ENSP00000362734.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-50                DA240339.1         31-80
            51-64               AL365504.33        151623-151636
            65-2189             AY720432.1         1-2125
            2190-2190           AL445489.10        14032-14032
            2191-3922           AY720432.1         2127-3858
            3923-4883           AK124819.1         24-984
            4884-5613           AK124819.1         986-1715
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q33.3"
     gene            1..5613
                     /gene="CRB2"
                     /gene_synonym="FSGS9; VMCKD"
                     /note="crumbs cell polarity complex component 2"
                     /db_xref="GeneID:286204"
                     /db_xref="HGNC:HGNC:18688"
                     /db_xref="MIM:609720"
     exon            1..158
                     /gene="CRB2"
                     /gene_synonym="FSGS9; VMCKD"
                     /inference="alignment:Splign:2.1.0"
     CDS             65..3922
                     /gene="CRB2"
                     /gene_synonym="FSGS9; VMCKD"
                     /note="crumbs-like protein 2; protein crumbs homolog 2;
                     crumbs family member 2; crumbs 2, cell polarity complex
                     component"
                     /codon_start=1
                     /product="protein crumbs homolog 2 precursor"
                     /protein_id="NP_775960.4"
                     /db_xref="CCDS:CCDS6852.2"
                     /db_xref="GeneID:286204"
                     /db_xref="HGNC:HGNC:18688"
                     /db_xref="MIM:609720"
                     /translation="MALARPGTPDPQALASVLLLLLWAPALSLLAGTVPSEPPSACAS
                     DPCAPGTECQATESGGYTCGPMEPRGCATQPCHHGALCVPQGPDPTGFRCYCVPGFQG
                     PRCELDIDECASRPCHHGATCRNLADRYECHCPLGYAGVTCEMEVDECASAPCLHGGS
                     CLDGVGSFRCVCAPGYGGTRCQLDLDECQSQPCAHGGTCHDLVNGFRCDCAGTGYEGT
                     HCEREVLECASAPCEHNASCLEGLGSFRCLCWPGYSGELCEVDEDECASSPCQHGGRC
                     LQRSDPALYGGVQAAFPGAFSFRHAAGFLCHCPPGFEGADCGVEVDECASRPCLNGGH
                     CQDLPNGFQCHCPDGYAGPTCEEDVDECLSDPCLHGGTCSDTVAGYICRCPETWGGRD
                     CSVQLTGCQGHTCPLAATCIPIFESGVHSYVCHCPPGTHGPFCGQNTTFSVMAGSPIQ
                     ASVPAGGPLGLALRFRTTLPAGTLATRNDTKESLELALVAATLQATLWSYSTTVLVLR
                     LPDLALNDGHWHQVEVVLHLATLELRLWHEGCPARLCVASGPVALASTASATPLPAGI
                     SSAQLGDATFAGCLQDVRVDGHLLLPEDLGENVLLGCERREQCRPLPCVHGGSCVDLW
                     THFRCDCARPHRGPTCADEIPAATFGLGGAPSSASFLLQELPGPNLTVSFLLRTRESA
                     GLLLQFANDSAAGLTVFLSEGRIRAEVPGSPAVVLPGRWDDGLRHLVMLSFGPDQLQD
                     LGQHVHVGGRLLAADSQPWGGPFRGCLQDLRLDGCHLPFFPLPLDNSSQPSELGGRQS
                     WNLTAGCVSEDMCSPDPCFNGGTCLVTWNDFHCTCPANFTGPTCAQQLWCPGQPCLPP
                     ATCEEVPDGFVCVAEATFREGPPAAFSGHNASSGRLLGGLSLAFRTRDSEAWLLRAAA
                     GALEGVWLAVRNGSLAGGVRGGHGLPGAVLPIPGPRVADGAWHRVRLAMERPAATTSR
                     WLLWLDGAATPVALRGLASDLGFLQGPGAVRILLAENFTGCLGRVALGGLPLPLARPR
                     PGAAPGAREHFASWPGTPAPILGCRGAPVCAPSPCLHDGACRDLFDAFACACGPGWEG
                     PRCEAHVDPCHSAPCARGRCHTHPDGRFECRCPPGFGGPRCRLPVPSKECSLNVTCLD
                     GSPCEGGSPAANCSCLEGLAGQRCQVPTLPCEANPCLNGGTCRAAGGVSECICNARFS
                     GQFCEVAKGLPLPLPFPLLEVAVPAACACLLLLLLGLLSGILAARKRRQSEGTYSPSQ
                     QEVAGARLEMDSVLKVPPEERLI"
     exon            159..482
                     /gene="CRB2"
                     /gene_synonym="FSGS9; VMCKD"
                     /inference="alignment:Splign:2.1.0"
     exon            483..678
                     /gene="CRB2"
                     /gene_synonym="FSGS9; VMCKD"
                     /inference="alignment:Splign:2.1.0"
     exon            679..818
                     /gene="CRB2"
                     /gene_synonym="FSGS9; VMCKD"
                     /inference="alignment:Splign:2.1.0"
     exon            819..1004
                     /gene="CRB2"
                     /gene_synonym="FSGS9; VMCKD"
                     /inference="alignment:Splign:2.1.0"
     exon            1005..1118
                     /gene="CRB2"
                     /gene_synonym="FSGS9; VMCKD"
                     /inference="alignment:Splign:2.1.0"
     exon            1119..1991
                     /gene="CRB2"
                     /gene_synonym="FSGS9; VMCKD"
                     /inference="alignment:Splign:2.1.0"
     exon            1992..2500
                     /gene="CRB2"
                     /gene_synonym="FSGS9; VMCKD"
                     /inference="alignment:Splign:2.1.0"
     exon            2501..2666
                     /gene="CRB2"
                     /gene_synonym="FSGS9; VMCKD"
                     /inference="alignment:Splign:2.1.0"
     exon            2667..3453
                     /gene="CRB2"
                     /gene_synonym="FSGS9; VMCKD"
                     /inference="alignment:Splign:2.1.0"
     exon            3454..3570
                     /gene="CRB2"
                     /gene_synonym="FSGS9; VMCKD"
                     /inference="alignment:Splign:2.1.0"
     exon            3571..3697
                     /gene="CRB2"
                     /gene_synonym="FSGS9; VMCKD"
                     /inference="alignment:Splign:2.1.0"
     exon            3698..5613
                     /gene="CRB2"
                     /gene_synonym="FSGS9; VMCKD"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      5613
                     /gene="CRB2"
                     /gene_synonym="FSGS9; VMCKD"
                     /note="major polyA site"
ORIGIN      
        1 gcggagccag ccaggccgcc ctcccgttct cacagcagcc gagcagagcg cagagcgggc
       61 tgccatggcg ctggccaggc ctgggacccc ggacccccag gccctggcct ctgtcctgct
      121 actgctgctc tgggcccctg ccctttccct cctggctggg acggtgcctt cagagccccc
      181 cagtgcctgt gcctcagacc cgtgcgctcc agggaccgag tgccaggcta ccgagagtgg
      241 tggctatacc tgtgggccca tggagccccg gggctgtgcc acccagccat gccaccacgg
      301 cgctctgtgt gtgccccagg gtccagatcc caccggcttc cgctgctact gcgtgccggg
      361 tttccagggc ccacgctgcg agctggacat cgatgagtgt gcatcccggc cgtgccacca
      421 tggggccacc tgccgcaacc tggccgatcg ctacgagtgc cattgccccc ttggctatgc
      481 aggcgtgacc tgcgagatgg aggtggacga gtgcgcctca gcgccctgcc tgcacggggg
      541 ctcgtgcctg gacggcgtgg gctccttccg ctgtgtgtgc gcgccaggct acgggggcac
      601 ccgttgccag ctggacctcg acgagtgcca gagccagccg tgcgcacatg ggggcacgtg
      661 ccacgacctg gtcaacgggt tccggtgcga ctgcgcgggc accggctacg agggcacgca
      721 ctgcgagcgg gaggtgctgg agtgcgcatc ggcgccctgc gagcacaacg cgtcctgcct
      781 cgagggcctc gggagcttcc gctgcctctg ttggccaggc tacagcggcg agctgtgcga
      841 ggtggacgag gacgagtgtg catcgagccc ctgccagcat gggggccgat gcctgcagcg
      901 ctctgacccg gccctctacg ggggtgtcca ggccgccttc cctggcgcct tcagcttccg
      961 ccatgctgcg ggtttcctgt gccactgccc tcctggcttt gagggagccg actgcggtgt
     1021 ggaggtggac gagtgtgcct cacggccatg cctcaacgga ggccactgcc aggacctgcc
     1081 caatggcttc cagtgtcact gcccagatgg ctacgcaggg ccgacatgtg aggaagatgt
     1141 ggatgaatgc ctgtcggatc cctgcctgca cggcggaacc tgcagtgaca ctgtggcagg
     1201 ctatatctgc aggtgcccag agacctgggg tgggcgcgac tgttctgtgc agctcactgg
     1261 ctgccagggc cacacctgcc cgctggctgc cacctgcatc cctatcttcg agtctggggt
     1321 ccacagttac gtctgccact gcccacctgg tacccatgga ccgttctgtg gccagaatac
     1381 caccttctct gtgatggctg ggagccccat tcaggcatca gtgccagctg gtggccccct
     1441 gggtctggca ctgaggtttc gcaccacact gcccgctggg accttggcca ctcgcaatga
     1501 caccaaggaa agcttggagc tggcattggt ggcagccaca cttcaggcca cactctggag
     1561 ctacagcacc actgtgcttg tcctgagact gccggacctg gccctaaacg atggccattg
     1621 gcaccaggtg gaggttgtgc tccatctagc gaccctggag ctacggctct ggcatgaggg
     1681 ctgccctgcc cggctctgtg tggcctctgg tcctgtggcc ctggcttcca cggcttcggc
     1741 aactccgctg cctgccggga tctcctctgc ccagctgggg gacgcgacct ttgcaggctg
     1801 cctccaggac gtgcgtgtgg atggccacct cctgctgcct gaggatctcg gtgagaacgt
     1861 cctcctgggc tgtgagcgcc gagagcagtg ccggcctctg ccttgtgtcc acggagggtc
     1921 ctgtgtggat ctgtggactc atttccgttg cgactgtgcc cggccccata gaggtcccac
     1981 gtgcgctgat gagattcctg ctgccacctt tggcttggga ggcgccccaa gctctgcctc
     2041 ctttctgctc caagagctgc caggtcccaa cctcacagtg tctttccttc tccgcactcg
     2101 ggagtccgct ggcctgttgc tccagtttgc caatgactcc gcagctggcc taacagtatt
     2161 cctgagtgag ggtcggatcc gggctgaggt gccgggcagt cctgctgtag tgctccctgg
     2221 gcgctgggat gatgggctcc gtcacctggt gatgctcagc ttcgggcctg accagctgca
     2281 ggacctgggg cagcacgtgc acgtgggtgg gaggctcctt gctgccgaca gccagccctg
     2341 gggtgggccc ttccgaggct gcctccagga cctgcgactc gatggctgcc acctcccctt
     2401 ctttcctctg ccactggata actcaagcca gcccagcgag ctcggcggca ggcagtcctg
     2461 gaacctcact gcgggctgcg tctccgagga catgtgcagt cctgacccct gtttcaatgg
     2521 tgggacttgc ctcgtcacct ggaatgactt ccactgtacc tgccctgcca atttcacggg
     2581 gcctacgtgt gcccagcagc tgtggtgtcc cggccagccc tgtctcccac ctgccacgtg
     2641 tgaggaggtc cctgatggct ttgtgtgtgt ggcggaggcc acgttccgcg agggtccccc
     2701 cgccgcgttc agcgggcaca acgcgtcgtc agggcgcttg ctcggcggcc tgtcgctggc
     2761 ctttcgcacg cgcgactccg aggcctggct gctgcgtgcc gcggcgggcg ccctggaagg
     2821 cgtgtggctg gcggtgcgca atggctcgct ggcggggggc gtgcgcggag gccatggcct
     2881 gcccggcgct gtgctgccca taccggggcc gcgcgtggcc gatggtgcct ggcaccgcgt
     2941 gcgtctggcc atggagcgcc cggcggccac cacctcgcgc tggctgctgt ggctggatgg
     3001 tgccgccacc ccggtggcgc tgcgcggcct ggccagtgac ctgggcttcc tgcagggccc
     3061 gggtgctgtg cgcatcctgc tggctgagaa cttcaccggc tgcttgggcc gcgtggcgct
     3121 gggcggcctg cccctgccct tggcgcggcc ccggcccggc gcggcccctg gcgcccgaga
     3181 gcacttcgcg tcttggcctg ggacgccggc cccgatcctc ggctgccgcg gcgcgcccgt
     3241 gtgtgcgccc tcgccctgtc tgcacgacgg tgcctgccgt gacctcttcg acgcctttgc
     3301 ctgcgcctgc ggcccggggt gggaaggccc gcgctgcgaa gcccacgtcg acccctgtca
     3361 ctccgccccc tgcgcccgtg gccgctgtca cacgcacccc gacggccgct tcgagtgccg
     3421 ctgcccgcct ggcttcgggg gcccgcgctg caggttgcct gtcccatcca aggagtgcag
     3481 cctgaatgtc acctgcctcg atggcagccc atgtgagggt ggctctcccg ctgccaactg
     3541 cagctgcctg gagggtcttg ctggccagag gtgtcaggtc cccactctcc cctgtgaagc
     3601 caacccctgc ttgaatgggg gcacctgccg ggcagctgga ggggtgtctg aatgtatctg
     3661 caatgccaga ttctccggcc agttctgtga agtggcgaag ggcctgcccc tgccgctgcc
     3721 attcccactg ctggaggtgg ccgtacctgc agcctgtgcc tgcctcctcc tcctcctcct
     3781 gggcctcctt tcagggatcc tggcagcccg aaagcgccgc cagtctgagg gcacctacag
     3841 cccaagccag caggaggtgg ctggggcccg gctggagatg gacagtgtcc tcaaggtgcc
     3901 accggaggag agactcatct aggccagcct ggctgctggc accagcacct ggaggtcctg
     3961 aatggtttct acctggagac ccaaggaagc tgcttccagg gctcgggaca ttgctacgga
     4021 agtgtcccct tggctggcag cctctgcctc tgcctctgcc ccatcctgga tggaggacga
     4081 ggggagcaac tcagggaaac agaggcctag agaggctgcg gacttctcca tcccaccctc
     4141 ggggttccgc cttggcaggt gtacggctgt gcgtgggagg gcacacgtgg gttcacagtg
     4201 tgttcaggag tgtgtgtatc tggaggagtg tgtgtgtgag tgtgtacctg ggcctgtgtt
     4261 agtctgcaga tgctagtgtg agtgtgtcct gacatggctc cagggcgtgt ctgccgtgtt
     4321 tactgtgtgt ctatgactgt gatgggtgta gctgatccca ggaggtggcg gctgcgccat
     4381 ggggtcaacc attacagtcc tagggcaggg gcggcccaag gctgcatgtt ctccaggagg
     4441 ccaggccggg gttgcccagg cacctccttc cccgcctctg ggggctgctc ctgctgtgga
     4501 ggcagctggg aagtcaggga aggccactag cagaggctga gtgggcttct ggctcctaga
     4561 acaaatgtcc cttcaggcag gtctgtctgc cagaagccag agccagtcat gcgagggaac
     4621 cacagaccca cccgccccct cagccggagc agcccgaggg agcagaggag gggctgcctg
     4681 gagcttccca ccctgctgtg gtcatttgtc aaagggggaa ggcacccact gcctacctca
     4741 cagggctgtt gtgaggatca gagaggacga cagtggggaa agaatctgga agtcttcaac
     4801 tgccgtctga tgggaaggac cgtctgggtg tccttctggg atgaggatga cagagcaacc
     4861 cttctcctgc cctgaacccc cccagctcac ctgaccacct ctggttctcc agctccggtc
     4921 cttcctagca gcctggtgag ctcactcctt cccctgatga ctggctgcct ctacacagac
     4981 tcggcgagag gacttgaagg aagccctctg ggttgtctgc tgagtacagg ggctcagtga
     5041 acactggcgc tgcctctgag tcggggctgg gcctgcagag gccgactcag aggagactct
     5101 gctgcttgct cccagcccct tccccggcga tgcccatcac actgtgacct cccatccctg
     5161 aagggcacct gcctgagggc ctggcctcct tccagcttca tggacctgga gatgtgccct
     5221 ttcatccttc ctgcttccca ggccagtaga tccgtttaca cttttgggtc gacagtcagc
     5281 ttttcctttt ggttttggcg ggtcccagag gcatgggtgt ccagtccaat gtggggagcc
     5341 acgtgacaac gtgggggact gggacatggg actgggaagt cagcagacgc tgggatagag
     5401 agggccctga acaccaggct caggggcttg cttggtcctt atcctgtagg aggtgggacc
     5461 acctttccct gaactttctc tacaaccctt gggagcgtgg ggaggaggcg gctggttcca
     5521 gggtcagttt actaagttag agatttggaa aacctgtgtc agctgtaact cctaggatat
     5581 tttatgtgga acctaacatg cagatgaaag ctg
//
