LOCUS       NM_001144958            2704 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens calcium release activated channel regulator 2A
            (CRACR2A), transcript variant 1, mRNA.
ACCESSION   NM_001144958
VERSION     NM_001144958.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 2704)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 2704)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2704)
  AUTHORS   Miteva,K.T., Pedicini,L., Wilson,L.A., Jayasinghe,I., Slip,R.G.,
            Marszalek,K., Gaunt,H.J., Bartoli,F., Deivasigamani,S.,
            Sobradillo,D., Beech,D.J. and McKeown,L.
  TITLE     Rab46 integrates Ca2+ and histamine signaling to regulate selective
            cargo release from Weibel-Palade bodies
  JOURNAL   J Cell Biol 218 (7), 2232-2246 (2019)
   PUBMED   31092558
  REMARK    GeneRIF: These data demonstrate for the first time that a Rab
            GTPase, Rab46, integrates G protein and Ca(2+) signals to couple
            on-demand histamine signals to selective Weibel-Palade bodies
            (WPBs) trafficking.
REFERENCE   6  (bases 1 to 2704)
  AUTHORS   Wilson,L.A., McKeown,L., Tumova,S., Li,J. and Beech,D.J.
  TITLE     Expression of a long variant of CRACR2A that belongs to the Rab
            GTPase protein family in endothelial cells
  JOURNAL   Biochem Biophys Res Commun 456 (1), 398-402 (2015)
   PUBMED   25475730
  REMARK    GeneRIF: endothelial cells contain a long variant of CRACR2A which
            is an EF-hand-containing Rab protein that lacks impact on CRAC
            channels
REFERENCE   7  (bases 1 to 2704)
  AUTHORS   Lambert,B., Vandeputte,J., Remacle,S., Bergiers,I., Simonis,N.,
            Twizere,J.C., Vidal,M. and Rezsohazy,R.
  TITLE     Protein interactions of the transcription factor Hoxa1
  JOURNAL   BMC Dev Biol 12, 29 (2012)
   PUBMED   23088713
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 2704)
  AUTHORS   Kuparinen,T., Seppala,I., Jylhava,J., Marttila,S., Aittoniemi,J.,
            Kettunen,J., Viikari,J., Kahonen,M., Raitakari,O., Lehtimaki,T. and
            Hurme,M.
  TITLE     Genome-wide association study does not reveal major genetic
            determinants for anti-cytomegalovirus antibody response
  JOURNAL   Genes Immun 13 (2), 184-190 (2012)
   PUBMED   21993531
REFERENCE   9  (bases 1 to 2704)
  AUTHORS   Srikanth,S., Jung,H.J., Kim,K.D., Souda,P., Whitelegge,J. and
            Gwack,Y.
  TITLE     A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that
            stabilizes CRAC channels in T cells
  JOURNAL   Nat Cell Biol 12 (5), 436-446 (2010)
   PUBMED   20418871
  REMARK    GeneRIF: CRACR2A interacts directly with Orai1 and STIM1, forming a
            ternary complex that dissociates at elevated Ca(2+) concentrations;
            is a key regulator of CRAC channel-mediated SOCE
REFERENCE   10 (bases 1 to 2704)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DB214079.1 and AK304017.1.
            
            On May 17, 2019 this sequence version replaced NM_001144958.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK304017.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000440314.7/ ENSP00000409382.2
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-7                 DB214079.1         92-98
            8-2704              AK304017.1         1-2697
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p13.32"
     gene            1..2704
                     /gene="CRACR2A"
                     /gene_synonym="EFCAB4B; RAB46"
                     /note="calcium release activated channel regulator 2A"
                     /db_xref="GeneID:84766"
                     /db_xref="HGNC:HGNC:28657"
                     /db_xref="MIM:614178"
     exon            1..95
                     /gene="CRACR2A"
                     /gene_synonym="EFCAB4B; RAB46"
                     /inference="alignment:Splign:2.1.0"
     exon            96..364
                     /gene="CRACR2A"
                     /gene_synonym="EFCAB4B; RAB46"
                     /inference="alignment:Splign:2.1.0"
     exon            365..445
                     /gene="CRACR2A"
                     /gene_synonym="EFCAB4B; RAB46"
                     /inference="alignment:Splign:2.1.0"
     exon            446..709
                     /gene="CRACR2A"
                     /gene_synonym="EFCAB4B; RAB46"
                     /inference="alignment:Splign:2.1.0"
     CDS             482..2677
                     /gene="CRACR2A"
                     /gene_synonym="EFCAB4B; RAB46"
                     /note="isoform a is encoded by transcript variant 1;
                     EF-hand calcium-binding domain-containing protein 4B; CRAC
                     channel regulator 2A; calcium release-activated calcium
                     channel regulator 2A; EF-hand calcium binding domain 4B;
                     CRAC regulator 2A; Ca2+ release-activated Ca2+ (CRAC)
                     channel regulator 2A; ras-related protein Rab-46"
                     /codon_start=1
                     /product="EF-hand calcium-binding domain-containing
                     protein 4B isoform a"
                     /protein_id="NP_001138430.1"
                     /db_xref="CCDS:CCDS44803.1"
                     /db_xref="GeneID:84766"
                     /db_xref="HGNC:HGNC:28657"
                     /db_xref="MIM:614178"
                     /translation="MAAPDGRVVSRPQRLGQGSGQGPKGSGACLHPLDSLEQKETQEQ
                     TSGQLVMLRKAQEFFQTCDAEGKGFIARKDMQRLHKELPLSLEELEDVFDALDADGNG
                     YLTPQEFTTGFSHFFFSQNNPSQEDAGEQVAQRHEEKVYLSRGDEDLGDMGEDEEAQF
                     RMLMDRLGAQKVLEDESDVKQLWLQLKKEEPHLLSNFEDFLTRIISQLQEAHEEKNEL
                     ECALKRKIAAYDEEIQHLYEEMEQQIKSEKEQFLLKDTERFQARSQELEQKLLCKEQE
                     LEQLTQKQKRLEGQCTALHHDKHETKAENTKLKLTNQELARELERTSWELQDAQQQLE
                     SLQQEACKLHQEKEMEVYRVTESLQREKAGLLKQLDFLRERNKHLRDERDICFQKNKA
                     AKANTAASRASWKKRSGSVIGKYVDSRGILRSQSEEEEEVFGIPRRSSLGLSGYPLTE
                     EEPGTGEPGPGGPYPRPLRRIISVEEDPLPQLLDGGFEQPLSKCSEEEEVSDQGVQGQ
                     IPEAPPLKLTPTSPRGQPVGKEALCKEESSPSAPDRLFKIVFVGNSAVGKTSFLRRFC
                     EDRFSPGMAATVGIDYRVKTLNVDNSQVALQLWDTAGQERYRCITQQFFRKADGVIVM
                     YDLTDKQSFLSVRRWLSSVEEAVGDRVPVLLLGNKLDNEKEREVPRGLGEQLATENNL
                     IFYECSAYSGHNTKESLLHLARFLKEQEDTVREDTIQVGHPAKKKSCCG"
     exon            710..821
                     /gene="CRACR2A"
                     /gene_synonym="EFCAB4B; RAB46"
                     /inference="alignment:Splign:2.1.0"
     exon            822..1005
                     /gene="CRACR2A"
                     /gene_synonym="EFCAB4B; RAB46"
                     /inference="alignment:Splign:2.1.0"
     exon            1006..1152
                     /gene="CRACR2A"
                     /gene_synonym="EFCAB4B; RAB46"
                     /inference="alignment:Splign:2.1.0"
     exon            1153..1243
                     /gene="CRACR2A"
                     /gene_synonym="EFCAB4B; RAB46"
                     /inference="alignment:Splign:2.1.0"
     exon            1244..1339
                     /gene="CRACR2A"
                     /gene_synonym="EFCAB4B; RAB46"
                     /inference="alignment:Splign:2.1.0"
     exon            1340..1527
                     /gene="CRACR2A"
                     /gene_synonym="EFCAB4B; RAB46"
                     /inference="alignment:Splign:2.1.0"
     exon            1528..1599
                     /gene="CRACR2A"
                     /gene_synonym="EFCAB4B; RAB46"
                     /inference="alignment:Splign:2.1.0"
     exon            1600..1645
                     /gene="CRACR2A"
                     /gene_synonym="EFCAB4B; RAB46"
                     /inference="alignment:Splign:2.1.0"
     exon            1646..1752
                     /gene="CRACR2A"
                     /gene_synonym="EFCAB4B; RAB46"
                     /inference="alignment:Splign:2.1.0"
     exon            1753..2083
                     /gene="CRACR2A"
                     /gene_synonym="EFCAB4B; RAB46"
                     /inference="alignment:Splign:2.1.0"
     exon            2084..2216
                     /gene="CRACR2A"
                     /gene_synonym="EFCAB4B; RAB46"
                     /inference="alignment:Splign:2.1.0"
     exon            2217..2298
                     /gene="CRACR2A"
                     /gene_synonym="EFCAB4B; RAB46"
                     /inference="alignment:Splign:2.1.0"
     exon            2299..2413
                     /gene="CRACR2A"
                     /gene_synonym="EFCAB4B; RAB46"
                     /inference="alignment:Splign:2.1.0"
     exon            2414..2515
                     /gene="CRACR2A"
                     /gene_synonym="EFCAB4B; RAB46"
                     /inference="alignment:Splign:2.1.0"
     exon            2516..2592
                     /gene="CRACR2A"
                     /gene_synonym="EFCAB4B; RAB46"
                     /inference="alignment:Splign:2.1.0"
     exon            2593..2704
                     /gene="CRACR2A"
                     /gene_synonym="EFCAB4B; RAB46"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggagcggaaa gagtcgggcg gagcaggcgg ctgcttgaca gctgagagcc gagcgggcgg
       61 cgcgggcagg tccacgggaa gcctacacgc ccaaggtcat gccctgacca cagctgccct
      121 tcaggaggct ggcacggagc cacctggggc cattgggctg tttgtcaagg gtggggcagc
      181 agcaacttgc cactcttcct ccatgagccc agccagagcc tccactgggc acttagcaca
      241 agaaaaaccc tgcaggagcc ggatccagag cgctgggatt cagcaggagt gccaggagaa
      301 ttcaacacag gtgtgctgcc tcctctcctg ccaggtgtgt ggctgcacag gagtcttgct
      361 gatggtggaa ttcaagtcac cagtggccaa agtaccctcc aggtgtgaag agttgcaggt
      421 cctccgagga gccgggagct gcaaggttct gaaaaagact tcaagaaact gactaatcgc
      481 gatggctgcc cctgacggga gggtagtctc cagaccccag agacttggtc aggggtctgg
      541 ccaggggcca aaggggagtg gagcctgcct gcatcccctg gacagcctgg agcagaagga
      601 gactcaggag caaacgtcgg gccagctagt catgctgagg aaggcacagg agttctttca
      661 gacctgtgat gctgaaggca agggcttcat cgccaggaag gatatgcaga ggctgcataa
      721 ggagctaccg ctcagcctgg aggaactgga ggatgtgttt gatgccctgg atgctgatgg
      781 caatggctat ctgaccccac aggagttcac tactggattt agtcacttct tcttcagcca
      841 gaataaccca agtcaggaag atgcaggtga acaggtggcc cagcgccatg aagagaaggt
      901 gtatctgtcc agaggggatg aggatctggg cgacatgggc gaagatgagg aagcccagtt
      961 ccggatgctg atggacagac ttggagccca aaaggtgttg gaagatgaaa gtgatgtcaa
     1021 gcagctctgg ttgcagctga agaaggagga acctcattta ctgtccaact ttgaagactt
     1081 cctgaccaga atcatctccc agctccaaga agcccatgag gagaagaatg aactggagtg
     1141 tgccctaaaa aggaaaattg ctgcttatga tgaagaaatc cagcatctct atgaggagat
     1201 ggaacaacaa atcaaaagtg agaaggagca gtttctcctg aaggacacag agaggtttca
     1261 agcccgcagt caagagctgg agcagaaact gttatgtaag gagcaggagc tggagcagct
     1321 cacccagaag cagaaaaggc tggaaggtca gtgcacagcc ctgcatcatg acaagcatga
     1381 gaccaaggct gagaatacca agctgaaact cactaaccag gagctggccc gggagctgga
     1441 gcggacttcc tgggagctcc aggatgctca gcagcagttg gaaagcctcc agcaagaggc
     1501 ctgcaaactc caccaagaga aggagatgga agtgtaccgt gtgacggaga gtctacagcg
     1561 tgagaaggcc gggctcctca agcagctgga tttcctaagg gaaaggaaca agcaccttcg
     1621 ggatgaacgg gacatatgtt ttcagaaaaa taaggcagcc aaggcaaaca cagctgcttc
     1681 cagggcaagt tggaaaaaga gatctggctc tgtgataggc aaatatgtgg acagcagagg
     1741 gattcttagg agccagtcag aggaggagga ggaggtgttt ggcatcccaa ggagaagctc
     1801 cctgggcctg agtggatatc ccctaacaga agaggagcca ggaaccgggg agccagggcc
     1861 tgggggtccg tacccccggc cgctccgcag aatcatctcc gttgaagaag accccctgcc
     1921 ccagctcctg gatggtggct ttgagcaacc cctgagcaaa tgctcagaag aggaagaggt
     1981 ctctgaccag ggggtacagg gacaaatccc ggaggcccca cccttgaaac tcacccccac
     2041 atccccccga gggcagcctg ttggaaaaga agccctgtgt aaggaggaaa gctctccctc
     2101 tgcccctgac cggctcttca agattgtgtt cgtgggcaat tccgcggtgg ggaagacatc
     2161 cttcctgagg agattctgtg aggaccggtt ctccccaggc atggcggcca ctgtgggcat
     2221 tgattaccgt gtgaagacgt tgaatgtgga caactctcag gtggccctgc agctgtggga
     2281 cacggctggg caggagaggt accggtgcat cacccagcag ttcttcagaa aggcagatgg
     2341 tgtcatcgtc atgtacgatc tcacagacaa gcagtcgttc ctgtcggtcc ggcggtggct
     2401 gagcagcgtg gaggaagctg tgggagaccg ggtgcctgtt cttctgctgg gtaataagct
     2461 tgacaacgag aaggagcggg aagtcccccg gggcctcgga gagcagcttg ccacggagaa
     2521 caatctgatc ttctatgaat gcagcgccta ctctggtcac aacaccaaag agtccctgct
     2581 ccatctggcc aggttcctca aggagcaaga agacacagtg agagaggaca ccattcaggt
     2641 cggccaccct gctaagaaga aatcctgctg tggctgatag ggtcctacaa ggactgcccc
     2701 cagc
//
