LOCUS       NM_001393381            7044 bp    mRNA    linear   PRI 12-JUN-2024
DEFINITION  Homo sapiens capping protein inhibiting regulator of actin dynamics
            (CRACD), transcript variant 1, mRNA.
ACCESSION   NM_001393381 XM_024454158
VERSION     NM_001393381.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7044)
  AUTHORS   Chang,Y., Zhang,J., Huo,X., Qu,X., Xia,C., Huang,K., Xie,F.,
            Wang,N., Wei,X. and Jia,Q.
  TITLE     Substrate rigidity dictates colorectal tumorigenic cell stemness
            and metastasis via CRAD-dependent mechanotransduction
  JOURNAL   Cell Rep 38 (7), 110390 (2022)
   PUBMED   35172140
  REMARK    GeneRIF: Substrate rigidity dictates colorectal tumorigenic cell
            stemness and metastasis via CRAD-dependent mechanotransduction.
REFERENCE   2  (bases 1 to 7044)
  AUTHORS   Cui,A., Xue,Y., Wang,X., Huang,Y., Han,X., Li,X., Niu,D., Niu,S.,
            Zhao,Y., Yang,X. and Yu,W.
  TITLE     Knockdown of CRAD suppresses the growth and promotes the apoptosis
            of human lung cancer cells via Claudin 4
  JOURNAL   Biosci Rep 40 (10) (2020)
   PUBMED   33006362
  REMARK    GeneRIF: Knockdown of CRAD suppresses the growth and promotes the
            apoptosis of human lung cancer cells via Claudin 4.
REFERENCE   3  (bases 1 to 7044)
  AUTHORS   Zhou,M., Fan,J., Li,Z., Li,P., Sun,Y., Yang,Y., Zhou,X., Wang,J.,
            Wang,Y., Qi,H., Cai,W., Dai,X. and Hirsch,F.R.
  TITLE     Prognostic impact of tumor mutation burden and the mutation in
            KIAA1211 in small cell lung cancer
  JOURNAL   Respir Res 20 (1), 248 (2019)
   PUBMED   31699089
  REMARK    GeneRIF: The overall survival of small cell lung cancer patients
            carrying KIAA1211 mutation was significantly longer than those with
            wild-type KIAA1211.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7044)
  AUTHORS   Jung,Y.S., Wang,W., Jun,S., Zhang,J., Srivastava,M., Kim,M.J.,
            Lien,E.M., Shang,J., Chen,J., McCrea,P.D., Zhang,S. and Park,J.I.
  TITLE     Deregulation of CRAD-controlled cytoskeleton initiates mucinous
            colorectal cancer via beta-catenin
  JOURNAL   Nat Cell Biol 20 (11), 1303-1314 (2018)
   PUBMED   30361697
  REMARK    GeneRIF: Datam indicate that cancer-related regulator of actin
            dynamics (CRAD) as frequently mutated or transcriptionally
            downregulated and as a tumour suppressor in colorectal cancer
            (CRC).
            GeneRIF: CRAD (Cancer-related Regulator of Actin Dynamics,
            previously KIAA1211) binds to and inhibits capping proteins, which
            leads to actin polymerization. CRAD is ubiquitously expressed in
            epithelial cells and is required for the maintenance of epithelial
            cell integrity. Genetic ablation of this gene deregulates Wnt
            signaling and induces the development of tumors in mouse models.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC092627.2, AC022267.8 and AC068620.5.
            
            On Feb 5, 2021 this sequence version replaced XM_024454158.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038197.1782582.1,
                                           SRR14038196.3323866.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000682029.1/ ENSP00000507165.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-202               AC092627.2         50975-51176
            203-349             AC022267.8         80262-80408
            350-521             AC022267.8         173299-173470
            522-657             AC022267.8         199192-199327
            658-822             AC068620.5         11041-11205
            823-891             AC068620.5         14172-14240
            892-1074            AC068620.5         16703-16885
            1075-3724           AC068620.5         17546-20195
            3725-3915           AC068620.5         26883-27073
            3916-4078           AC068620.5         27610-27772
            4079-7044           AC068620.5         31150-34115
FEATURES             Location/Qualifiers
     source          1..7044
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q12"
     gene            1..7044
                     /gene="CRACD"
                     /gene_synonym="CRAD; KIAA1211"
                     /note="capping protein inhibiting regulator of actin
                     dynamics"
                     /db_xref="GeneID:57482"
                     /db_xref="HGNC:HGNC:29219"
                     /db_xref="MIM:618327"
     exon            1..202
                     /gene="CRACD"
                     /gene_synonym="CRAD; KIAA1211"
                     /inference="alignment:Splign:2.1.0"
     exon            203..349
                     /gene="CRACD"
                     /gene_synonym="CRAD; KIAA1211"
                     /inference="alignment:Splign:2.1.0"
     exon            350..521
                     /gene="CRACD"
                     /gene_synonym="CRAD; KIAA1211"
                     /inference="alignment:Splign:2.1.0"
     exon            522..657
                     /gene="CRACD"
                     /gene_synonym="CRAD; KIAA1211"
                     /inference="alignment:Splign:2.1.0"
     CDS             538..4239
                     /gene="CRACD"
                     /gene_synonym="CRAD; KIAA1211"
                     /note="isoform 1 is encoded by transcript variant 1;
                     cancer-related regulator of actin dynamics"
                     /codon_start=1
                     /product="capping protein-inhibiting regulator of actin
                     dynamics isoform 1"
                     /protein_id="NP_001380310.1"
                     /db_xref="CCDS:CCDS43230.1"
                     /db_xref="GeneID:57482"
                     /db_xref="HGNC:HGNC:29219"
                     /db_xref="MIM:618327"
                     /translation="MGTRAFSHDSIFIPDGGAESEQTVQAMSQDNILGKVKTLQQQLG
                     KNIKFGQRSPNAIPMNKANSGEASLEEDLFLTSPMEIVTQQDIVLSDAENKSSDTPSS
                     LSPLNLPGAGSEMEEKVAPVKPSRPKRHFSSAGTIESVNLDAIPLAIARLDNSAAKHK
                     LAVKPKKQRVSKKHRRLAQDPQHEQGGLESRPCLDQNGHPGEDKPTWHEEEPNPLDSE
                     EERRRQEDYWRELEAKCKRQKAEAAEKRRLEEQRLQALERRLWEENRRQELLEEEGEG
                     QEPPLEAERAPREEQQRSLEAPGWEDAERREREERERLEAEEERRRLQAQAQAEERRR
                     LEEDARLEERRRQEEEEGRCAEELKRQEEEEAEGWEELEQQEAEVQGPPEALEETGEG
                     RRGAEEEDLGEEEEEGQAHLEDWRGQLSELLNDFEERLEDQERLKPEGQREHSEEPGI
                     CEEQNPEAERRREQQGRSGDFQGADRPGPEEKREEGDTEPLLKQEGPVEAAQPPVERK
                     EAAALEQGRKVEELRWQEVDERQTMPRPYTFQVSSGGKQILFPKVNLSPVTPAKDTGL
                     TAAPQEPKAPKASPVQHALPSSLSVPHTAILVTGAQLCGPAVNLSQIKDTACKSLLGL
                     EEKKHAEAPAGENPPRGPGDARAGSGKAKPRQESPSSASALAEWASIRSRILKNAESD
                     PRSSERDQLRPGDESTPRGRCDSRGNQRKTPPVNAKFSIMPAWQKFSDGGTETSKQST
                     EAESIRKRPMLGPSEETAPQPPPAGVRELGKGPEKSEMHREPADTTEGCKFAKDLPSF
                     LVPSLPYPPQKVVAHTEFTTSSDSETANGIAKPDPVMPGGEEKASPFGIKLRRTNYSL
                     RFNCDQQAEQKKKKRHSSTGDSADAGPPAAGSARGEKEMEGVALKHGPSLPQERKQAP
                     STRRDSAEPSSSRSVPVAHPGPPPASSQTPAPEHDKAANKMPLAQKPALAPKPTSQTP
                     PASPLSKLSRPYLVELLSRRAGRPDPEPSEPSKEDQESSDRRPPSPPGPEERKGQKRD
                     EEEEATERKPASPPLPATQQEKPSQTPEAGRKEKPMLQSRHSLDGSKLTEKVETAQPL
                     WITLALQKQKGFREQQATREERKQAREAKQAEKLSKENVSVSVQPGSSSVSRAGSLHK
                     STALPEEKRPETAVSRLERREQLKKANTLPTSVTVEISDSAPPAPLVKEVTKRFSTPD
                     AAPVSTEPAWLALAKRKAKAWSDCPQIIK"
     exon            658..822
                     /gene="CRACD"
                     /gene_synonym="CRAD; KIAA1211"
                     /inference="alignment:Splign:2.1.0"
     exon            823..891
                     /gene="CRACD"
                     /gene_synonym="CRAD; KIAA1211"
                     /inference="alignment:Splign:2.1.0"
     exon            892..1074
                     /gene="CRACD"
                     /gene_synonym="CRAD; KIAA1211"
                     /inference="alignment:Splign:2.1.0"
     exon            1075..3724
                     /gene="CRACD"
                     /gene_synonym="CRAD; KIAA1211"
                     /inference="alignment:Splign:2.1.0"
     exon            3725..3915
                     /gene="CRACD"
                     /gene_synonym="CRAD; KIAA1211"
                     /inference="alignment:Splign:2.1.0"
     exon            3916..4078
                     /gene="CRACD"
                     /gene_synonym="CRAD; KIAA1211"
                     /inference="alignment:Splign:2.1.0"
     exon            4079..7044
                     /gene="CRACD"
                     /gene_synonym="CRAD; KIAA1211"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5850..5855
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CRACD"
                     /gene_synonym="CRAD; KIAA1211"
                     /note="hexamer: AATAAA"
     polyA_site      5872
                     /gene="CRACD"
                     /gene_synonym="CRAD; KIAA1211"
                     /note="major polyA site"
     regulatory      7025..7030
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CRACD"
                     /gene_synonym="CRAD; KIAA1211"
                     /note="hexamer: AATAAA"
     polyA_site      7044
                     /gene="CRACD"
                     /gene_synonym="CRAD; KIAA1211"
ORIGIN      
        1 gcagtgccgc gcggtggcgg cagtggggag agcaatggag gccacggccg actgagaggc
       61 gggtgcgctg ctggtgctgc tgccgcggcg gctgctgatg cggaggctgc cgcgcgcagt
      121 gccgccgagt ccgccgcccg cccgggaccg ccggtcgccg gccagccgct ctgccagccg
      181 gagcgccagg cggggacctc agcttacaag agcagtgatt cccaggcttt ctcagcaatg
      241 taccccattt cctgacattt acagctgaag gtgaagtttc ccttttgcaa ggagaaaatt
      301 ttggtggaga attatccaac aagaaaaaga cctgtggcta ttaagataga caagaagaag
      361 ggaggcaaat tccagccttt taaaaagttg tttggcaaaa ggaaaaagaa agacccttcg
      421 ttgttccggg tgccgtcgtt ggggaagaag agttactctc accagagtgt cagcaatggg
      481 accttctctt cggatgagga gaccctggaa gacaatctaa ggtccttcaa ctattctatg
      541 ggaacccggg cattttccca tgacagtatt tttatccctg atgggggagc agaaagtgag
      601 cagacagttc aagcaatgtc acaggacaac atcctgggca aagtcaaaac tcttcagcaa
      661 cagttgggca agaatatcaa gtttgggcag cggtcaccca atgccattcc catgaataag
      721 gcaaacagtg gagaggctag cttagaagag gatctgttcc tgaccagtcc catggaaatt
      781 gtgactcagc aggacatcgt cctctcagac gcagagaaca agtccagtga tacgccaagt
      841 tctctaagtc ctctgaatct ccctggagct ggaagtgaga tggaagagaa ggttgctccc
      901 gttaaaccgt ctcggccaaa aaggcacttc tcttctgctg gcaccatcga aagtgtcaac
      961 ttagatgcca tccccctggc catcgctcgc ctggacaaca gtgccgccaa gcacaagctg
     1021 gctgttaagc caaaaaaaca gagggtgtca aagaagcaca ggcgccttgc ccaggatcca
     1081 caacatgagc aaggcggcct tgagagtcgg ccctgcctgg accagaacgg acacccaggc
     1141 gaggacaagc caacgtggca cgaagaggaa cccaatccgc tggattccga ggaagagaga
     1201 agacgccaag aagactactg gcgagaactg gaggccaagt gcaagcggca aaaggcggaa
     1261 gcagccgaga agagacgcct agaggagcag aggctgcagg cgctggagag gaggctttgg
     1321 gaagagaaca gaaggcagga gctcttggag gaggagggcg aggggcagga gccgcctcta
     1381 gaggcggaaa gggcgccgcg ggaagagcag cagcggagcc tggaagcgcc aggttgggag
     1441 gacgcggagc ggagggagcg tgaggagcgc gagcgcctgg aggcggagga ggagcgaagg
     1501 cgtctgcagg cccaggccca agcggaggag aggcggcggc tggaggagga cgccaggctg
     1561 gaggagcgga ggcggcagga ggaggaggaa ggaagatgcg cggaggagct caaaaggcag
     1621 gaggaggagg aggctgaggg atgggaagag ctggaacagc aggaggcgga ggtgcagggg
     1681 ccgcccgagg cgttggagga gactggggag ggccggcggg gcgcggagga ggaggatctg
     1741 ggggaagagg aggaggaggg ccaggcgcac ctggaggact ggagggggca gctcagtgag
     1801 cttctgaacg actttgagga gaggctcgaa gaccaggaac gcctgaaacc cgaaggacaa
     1861 agagaacact ccgaggagcc aggtatttgc gaggagcaga acccagaggc cgagcggcga
     1921 agagagcagc agggaaggag cggggatttc cagggggccg atcgtcctgg gcccgaggaa
     1981 aagagagaag aaggggacac ggagcctctc ctgaaacaag aggggccggt ggaagccgcg
     2041 cagcctccgg tggagaggaa agaagccgcc gcccttgaac aaggccgcaa ggtggaggag
     2101 ctgcggtggc aggaggtgga cgagagacag accatgcccc ggccctacac gttccaggtg
     2161 tcctccggag ggaagcagat tctctttccc aaagtcaacc tgagccccgt gacgcccgca
     2221 aaggacacgg ggctcaccgc tgctccccag gaaccaaagg cccccaaagc cagcccagtc
     2281 cagcacgccc taccgtcgtc cctgagcgtt ccccacaccg ccattctggt cacgggcgcg
     2341 cagctctgtg gcccggcagt caacctgagc cagatcaagg acaccgcgtg caagtccctc
     2401 ctgggcttgg aggagaagaa gcacgcggaa gccccagctg gggagaaccc tccccgaggc
     2461 cccggcgacg cgagggcggg cagcgggaag gctaagcccc gccaggagtc tcccagcagc
     2521 gcgtccgcac tcgcagaatg ggcttccatt cggtccagaa tcctgaagaa cgcagagagt
     2581 gacccgcgca gcagcgagag ggaccagttg aggcccggtg atgagtccac tcccaggggc
     2641 cggtgtgatt cccgcgggaa ccaacggaag actccgccag tcaatgcaaa gttctctatt
     2701 atgcctgcct ggcagaaatt ttccgatggt ggcacggaga cctccaaaca gagcacggaa
     2761 gctgaaagca tacgaaaaag acccatgctg ggacccagcg aagagacagc cccccagcct
     2821 cctcctgctg gtgttcgcga gctcgggaag ggtccggaga agtcggagat gcaccgggag
     2881 cccgcagaca ccaccgaggg atgcaaattt gccaaagacc tcccgtcttt ccttgtccca
     2941 agccttcctt accctccgca gaaagtggtg gcccacacag agttcacgac ctcgtcggac
     3001 agcgagactg caaacgggat agcaaagcca gaccctgtga tgccaggtgg agaggaaaaa
     3061 gcctcaccgt ttggaataaa attgagaagg accaactatt ccttgcgctt caactgcgac
     3121 caacaggcag aacagaagaa gaagaagagg cacagcagca ccggagacag cgcggatgca
     3181 gggccgcctg cagcggggag cgctcgtgga gagaaagaga tggagggtgt ggccctcaag
     3241 catggtccat ccctccccca agagcggaag caagcccctt ccacccggag ggactccgct
     3301 gaaccttcca gcagccgctc tgttcctgtg gcccaccctg ggcctccacc ggccagcagc
     3361 cagaccccgg ctccggagca cgacaaggca gcaaacaaaa tgccactggc acaaaagcca
     3421 gcactggctc ccaagcccac cagtcagacc ccaccagcat ccccactttc caaactgagc
     3481 aggccctact tggtagagct gctgtctcgc cgagcgggga ggccggaccc agagccaagt
     3541 gagccgtcca aggaggacca ggagagcagt gaccgccggc caccctcgcc cccaggcccc
     3601 gaggaaagga agggacagaa gagggacgag gaggaagagg cgacagagag gaaacctgct
     3661 tccccacctc tgcctgccac tcagcaagag aaaccttctc aaacacccga ggccgggagg
     3721 aaagagaagc cgatgcttca gagcagacac tccttagatg gctccaaact tacagagaaa
     3781 gtggaaactg ctcagccgct gtggataacg ttagcactgc aaaagcaaaa ggggtttcgg
     3841 gagcagcagg cgacgcggga ggagagaaag caagccagag aggccaaaca ggcagaaaag
     3901 ctctccaaag aaaatgtcag tgtcagcgtg cagcctggaa gcagcagtgt cagcagagca
     3961 ggttccctgc acaagtccac tgctctgcca gaagagaaga ggcccgagac tgcagtgtcc
     4021 aggcttgagc gcagagaaca gctgaaaaag gccaatactc ttcctacgtc tgtgacagtg
     4081 gagatctccg actcggctcc cccagcgccg ctggtaaaag aagtcaccaa gaggttttcc
     4141 accccggatg ctgcccccgt gtcaacagaa ccagcctggc tggctttggc caaaaggaaa
     4201 gcaaaggctt ggagcgactg tccacagatt attaagtaaa gagtgactct cacccatccc
     4261 tactgccagt tattggctcc tctcttgccc tttttattta tttatttttt atatggggta
     4321 aagaaatcaa gctagggaaa agaagcatgt tttataggct ccaaaataat gtttagaaac
     4381 tgaactggtg gtgttcattg taaagagtgg atttgcacaa ccctaggtcc tggtgcctcg
     4441 agctcctcct agttctcaag agaaaattcc gtccacagga ccacatgaag caaaatctct
     4501 aagctaagaa ctcgtgagag cctgccatgc ccatttcatg gccttgcaca cacacccacc
     4561 cacacaccat tcagaacatg tacttttgcc aacctttggt aatggttttt tgattctatg
     4621 cactaatttt ctttgtccaa aacatttact ctaccatatg tctgggaatg tttatccttt
     4681 ctacagtaat ggtgaaagga tcatatctag ctaaaagcaa gaacacccat tctcctgaat
     4741 gcagtgagta attgggaatc acaaggaaca ttctggcacc caactgtgcc catcttagtt
     4801 ttcatgagtt ttccccactc tgaagctgta acccagaagg cacatccatt cacattttta
     4861 ccgcactgtg gattcatagt gtggggccct gttattccaa taccctcctt aaggacatgt
     4921 gaagcatttg gcccagttgg ctcccaggga acattttaat ttaatgtagt gaagaaagac
     4981 aattctagat cagagctaca agttcacttt ctgtctctga gaatctccat ctagggcagt
     5041 agttcttatg atgtgtaggc tccctcctag ggcctactca atcagagcct gtggggactg
     5101 ggccaggaat atgtattctg gcaaaaactc ttcaggtcag ttctgataga tcccaaaggt
     5161 taagaactgt gagaaacact gatcctaatg caatctaatt tttcagactt cgaaggggag
     5221 gctcattcac tcaaaaagca acagtgagac aggaaaaata ataaaacaac cccccaagcc
     5281 agccatttat tacaagaagc aacaggttat tgacattaca tgtttgaaaa ttccctttgg
     5341 tctttaggga aaataaacag gaagccaaga tttggagcct ttgtaataag gacttcctgc
     5401 agaaagtctt ttctttacta taattgagta attcatattt agagtcacat gtccagtagc
     5461 atttctaatt ttgagcattc accttgctac ctttaaaaaa catctgagtt ttaagtggcc
     5521 tttttatcat catacacatg tgcatacaaa gaagggactt ggcagtttaa aagccacata
     5581 tattcacttt tattgcccta aatttacatg aaacagacat actggcaaac tcacatattg
     5641 ctggtgctaa ccttatattt catagtgttg gcatattccc cttttcttag attcttactc
     5701 cgaaatatag gtacacatcc tttgctctgt gcagagggaa ttacatcctt tttcctctcc
     5761 tacaaaaaca tgctttatta agtatccatc attactttcc tttatgctcg ctcaatatgc
     5821 aatgtgctgt tattctacca tgtaccttaa ataaaggatg atggcaaagt tatttaccat
     5881 gtagaaacca ttttctttct agaaacaata gctcagcctc actgtagcag ctggcatgtg
     5941 tggtcaagtg gatagttgta ctcttgcaag ttggatttaa tatcatatat actggacctt
     6001 cagactgtta aaaatcaatg taaccttttt ttattgctat ggcaagcaat tagtatttca
     6061 ctgcacgtct tccatactaa tgttcatttc taaatcttat atgtaggcat ttgttagttc
     6121 caatgatttc ctcactaata taacactttt taatgggaat ctttccacct acagccctgg
     6181 aatgataatg ctacagtaat tcttctgaat tgactttttc tttcatcctg tcagctttgg
     6241 acaatatccc aattatggca gggaacaggt ggggaactaa gatcagttac aaaaagttgt
     6301 agatgtgtca actttgtatt ggctgggata tcactgtgcc caaacaaaac aggcgaaata
     6361 cctcagttaa aatttttcca tcaaagtctt taaaagaaga gtatactgaa gaaagggcag
     6421 tcacaatact tacttctaac agcttctaaa gggtacatgt ttaacatttc atttcaaaat
     6481 caccccaaat ttgcactaaa taccaatgaa gtgttatttt gctttagtag tcttctgagc
     6541 aacaaactat ggggaattct gtaaaaacat ataaaaagtt caagactttt tttttaaatg
     6601 aatgatcact atgttaaatg caaacttttt ttttttttta tttaaacaaa catacacttc
     6661 tcctggcaag gttatagatg attaacctct gttcatagac ttatatataa aactagaggg
     6721 ttttttgttt acttttttaa tttttcaagt gcaattgttt cttacacaga cattattact
     6781 attaaattat catttagcca gttatctgca aatatatagt atgtattgtc tcttcttgtg
     6841 acgtttagtt taattgctta ttttaaagca gaaacattag ttacaagtgt cttacaatat
     6901 ttttaccaac agtaaagtag agacttaatg aaaatacctt agtgtgattt taatataatt
     6961 tgcatatttt agttgtataa agttttaatg taaaatgtcc attattgaag ggaaaagatc
     7021 tttcaataaa aaatacccac gaga
//
