LOCUS       NM_001014447            2163 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens carboxypeptidase Z (CPZ), transcript variant 1, mRNA.
ACCESSION   NM_001014447
VERSION     NM_001014447.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Garcia-Pardo,J., Tanco,S., Garcia-Guerrero,M.C., Dasgupta,S.,
            Aviles,F.X., Lorenzo,J. and Fricker,L.D.
  TITLE     Substrate Specificity and Structural Modeling of Human
            Carboxypeptidase Z: A Unique Protease with a Frizzled-Like Domain
  JOURNAL   Int J Mol Sci 21 (22), 8687 (2020)
   PUBMED   33217972
  REMARK    GeneRIF: Substrate Specificity and Structural Modeling of Human
            Carboxypeptidase Z: A Unique Protease with a Frizzled-Like Domain.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Tang,J., Liu,W., Zhu,J., Zhang,J., Wang,F.H., Liang,J.H.,
            Zeng,J.H., Wang,H., Xia,H. and He,J.
  TITLE     RSRC1 and CPZ gene polymorphisms with neuroblastoma susceptibility
            in Chinese children
  JOURNAL   Gene 662, 83-87 (2018)
   PUBMED   29653227
  REMARK    GeneRIF: CPZ rs3796725 and rs3796727 were not associated with
            neuroblastoma risk in Chinese children..
REFERENCE   3  (bases 1 to 2163)
  AUTHORS   McDaniel,L.D., Conkrite,K.L., Chang,X., Capasso,M., Vaksman,Z.,
            Oldridge,D.A., Zachariou,A., Horn,M., Diamond,M., Hou,C.,
            Iolascon,A., Hakonarson,H., Rahman,N., Devoto,M. and Diskin,S.J.
  TITLE     Common variants upstream of MLF1 at 3q25 and within CPZ at 4p16
            associated with neuroblastoma
  JOURNAL   PLoS Genet 13 (5), e1006787 (2017)
   PUBMED   28545128
  REMARK    GeneRIF: Common variants within CPZ at 4p16 is associated with
            neuroblastoma.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2163)
  AUTHORS   Rual,J.F., Hirozane-Kishikawa,T., Hao,T., Bertin,N., Li,S.,
            Dricot,A., Li,N., Rosenberg,J., Lamesch,P., Vidalain,P.O.,
            Clingingsmith,T.R., Hartley,J.L., Esposito,D., Cheo,D., Moore,T.,
            Simmons,B., Sequerra,R., Bosak,S., Doucette-Stamm,L., Le Peuch,C.,
            Vandenhaute,J., Cusick,M.E., Albala,J.S., Hill,D.E. and Vidal,M.
  TITLE     Human ORFeome version 1.1: a platform for reverse proteomics
  JOURNAL   Genome Res 14 (10B), 2128-2135 (2004)
   PUBMED   15489335
REFERENCE   5  (bases 1 to 2163)
  AUTHORS   Fan,X., Olson,S.J., Blevins,L.S., Allen,G.S. and Johnson,M.D.
  TITLE     Immunohistochemical localization of carboxypeptidases D, E, and Z
            in pituitary adenomas and normal human pituitary
  JOURNAL   J Histochem Cytochem 50 (11), 1509-1516 (2002)
   PUBMED   12417617
REFERENCE   6  (bases 1 to 2163)
  AUTHORS   Reznik,S.E. and Fricker,L.D.
  TITLE     Carboxypeptidases from A to z: implications in embryonic
            development and Wnt binding
  JOURNAL   Cell Mol Life Sci 58 (12-13), 1790-1804 (2001)
   PUBMED   11766880
  REMARK    Review article
REFERENCE   7  (bases 1 to 2163)
  AUTHORS   Novikova,E.G., Reznik,S.E., Varlamov,O. and Fricker,L.D.
  TITLE     Carboxypeptidase Z is present in the regulated secretory pathway
            and extracellular matrix in cultured cells and in human tissues
  JOURNAL   J Biol Chem 275 (7), 4865-4870 (2000)
   PUBMED   10671522
REFERENCE   8  (bases 1 to 2163)
  AUTHORS   Novikova,E.G. and Fricker,L.D.
  TITLE     Purification and characterization of human metallocarboxypeptidase
            Z
  JOURNAL   Biochem Biophys Res Commun 256 (3), 564-568 (1999)
   PUBMED   10080937
REFERENCE   9  (bases 1 to 2163)
  AUTHORS   Song,L. and Fricker,L.D.
  TITLE     Cloning and expression of human carboxypeptidase Z, a novel
            metallocarboxypeptidase
  JOURNAL   J Biol Chem 272 (16), 10543-10550 (1997)
   PUBMED   9099699
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC105345.3, KF495771.2 and
            KF510872.1.
            
            On Nov 22, 2018 this sequence version replaced NM_001014447.2.
            
            Summary: This gene encodes a member of the metallocarboxypeptidase
            family. This enzyme displays carboxypeptidase activity towards
            substrates with basic C-terminal residues. It is most active at
            neutral pH and is inhibited by active site-directed inhibitors of
            metallocarboxypeptidases. Alternative splicing in the coding region
            results in multiple transcript variants encoding different
            isoforms. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes the longest protein
            (isoform 1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC080539.1, GQ891514.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000360986.9/ ENSP00000354255.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-157               AC105345.3         140949-141105
            158-190             AC105345.3         147637-147669
            191-436             KF495771.2         1-246               c
            437-565             AC105345.3         149553-149681
            566-778             AC105345.3         152160-152372
            779-975             AC105345.3         154173-154369
            976-1137            AC105345.3         154921-155082
            1138-1296           AC105345.3         155451-155609
            1297-1432           AC105345.3         160211-160346
            1433-1572           AC105345.3         162543-162682
            1573-1672           AC105345.3         166613-166712
            1673-2062           AC105345.3         167446-167835
            2063-2163           KF510872.1         266-366             c
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4p16.1"
     gene            1..2163
                     /gene="CPZ"
                     /note="carboxypeptidase Z"
                     /db_xref="GeneID:8532"
                     /db_xref="HGNC:HGNC:2333"
                     /db_xref="MIM:603105"
     exon            1..157
                     /gene="CPZ"
                     /inference="alignment:Splign:2.1.0"
     CDS             70..2028
                     /gene="CPZ"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; metallocarboxypeptidase Z; VEZT/CPZ fusion;
                     epididymis secretory sperm binding protein"
                     /codon_start=1
                     /product="carboxypeptidase Z isoform 1 precursor"
                     /protein_id="NP_001014447.2"
                     /db_xref="CCDS:CCDS33953.1"
                     /db_xref="GeneID:8532"
                     /db_xref="HGNC:HGNC:2333"
                     /db_xref="MIM:603105"
                     /translation="MPPPLPLLLLTVLVVAAARPGCEFERNPAGECHRPPAADSATCV
                     DLQLRTCSDAAYNHTTFPNLLQHRSWEVVEASSEYILLSVLHQLLEGQCNPDLRLLGC
                     AVLAPRCEGGWVRRPCRHICEGLREVCQPAFDAIDMAWPYFLDCHRYFTREDEGCYDP
                     LEKLRGGLEADEALPSGLPPTFIRFSHHSYAQMVRVLRRTASRCAHVARTYSIGRSFD
                     GRELLVIEFSSRPGQHELMEPEVKLIGNIHGNEVAGREMLIYLAQYLCSEYLLGNPRI
                     QRLLNTTRIHLLPSMNPDGYEVAAAEGAGYNGWTSGRQNAQNLDLNRNFPDLTSEYYR
                     LAETRGARSDHIPIPQHYWWGKVAPETKAIMKWMQTIPFVLSASLHGGDLVVSYPFDF
                     SKHPQEEKMFSPTPDEKMFKLLSRAYADVHPMMMDRSENRCGGNFLKRGSIINGADWY
                     SFTGGMSDFNYLHTNCFEITVELGCVKFPPEEALYILWQHNKESLLNFVETVHRGIKG
                     VVTDKFGKPVKNARISVKGIRHDITTAPDGDYWRLLPPGIHIVIAQAPGYAKVIKKVI
                     IPARMKRAGRVDFILQPLGMGPKNFIHGLRRTGPHDPLGGASSLGEATEPDPLRARRQ
                     PSADGSKPWWWSYFTSLSTHRPRWLLKY"
     exon            158..190
                     /gene="CPZ"
                     /inference="alignment:Splign:2.1.0"
     exon            191..565
                     /gene="CPZ"
                     /inference="alignment:Splign:2.1.0"
     exon            566..778
                     /gene="CPZ"
                     /inference="alignment:Splign:2.1.0"
     exon            779..975
                     /gene="CPZ"
                     /inference="alignment:Splign:2.1.0"
     exon            976..1137
                     /gene="CPZ"
                     /inference="alignment:Splign:2.1.0"
     exon            1138..1296
                     /gene="CPZ"
                     /inference="alignment:Splign:2.1.0"
     exon            1297..1432
                     /gene="CPZ"
                     /inference="alignment:Splign:2.1.0"
     exon            1433..1572
                     /gene="CPZ"
                     /inference="alignment:Splign:2.1.0"
     exon            1573..1672
                     /gene="CPZ"
                     /inference="alignment:Splign:2.1.0"
     exon            1673..2163
                     /gene="CPZ"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2141..2146
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CPZ"
                     /note="hexamer: ATTAAA"
     polyA_site      2163
                     /gene="CPZ"
                     /note="major polyA site"
ORIGIN      
        1 agagccccgg ccccgcgcgg cccgagtgcc acatcactgc gctggccgtc caaggtccgc
       61 cgccccacca tgccgccccc gctgccgctg ctgctcctta cagtcctggt cgtcgccgct
      121 gcccggccgg ggtgcgagtt tgagcggaac cccgccggtg aatgccacag gccaccagct
      181 gcagacagcg ccacctgcgt ggacctgcag ctcaggacct gcagcgatgc cgcctacaac
      241 cacaccacct tccccaacct gcttcagcac cggtcgtggg aggtggtgga ggccagctcc
      301 gagtacatcc tgctgagcgt tctacaccag ctcctggaag gccagtgcaa cccggacctg
      361 cggctgctgg gctgtgctgt gctggccccc cggtgtgagg gcggctgggt gcgcagaccc
      421 tgccggcaca tctgcgaggg cctgcgggag gtctgccagc ccgccttcga cgccattgac
      481 atggcctggc cctacttcct tgactgccac cgctacttca cgagagagga cgagggctgc
      541 tatgacccgc tggagaagct tcggggaggc ctggaggctg acgaggcact gccctcaggg
      601 ctgccgccca ccttcatccg cttcagccac cactcctacg cccagatggt gcgtgtgctg
      661 aggcggacgg cctcccgctg cgcccacgtg gccaggacct acagcatcgg gcgcagcttc
      721 gacggcaggg agctgctggt catcgagttc tccagccgcc ccggccagca cgagctgatg
      781 gagcccgagg tgaagctcat cggcaacatt catggcaacg aggtggcggg ccgggagatg
      841 ctcatctacc tagcccagta cctgtgctct gagtacctgc ttggtaaccc ccgcatccag
      901 cgcctgctca acaccacccg catccacctg ctgccctcca tgaaccctga cggctatgag
      961 gtggcagctg ccgagggtgc cggctacaac gggtggacga gcgggaggca gaacgcgcag
     1021 aacctggatc tgaaccgaaa tttcccggac ctgacgtccg agtactaccg gctggcggag
     1081 acccgcggcg cacgcagcga ccacatcccc atcccccagc actactggtg gggtaaggtg
     1141 gccccggaga caaaggcaat catgaagtgg atgcagacca taccctttgt gctctcagcc
     1201 agccttcatg ggggcgacct ggtggtgtcc taccccttcg acttctccaa gcacccccag
     1261 gaggagaaga tgttttctcc cacgcccgac gagaagatgt tcaagctgct gtccagagcc
     1321 tacgctgacg tccaccccat gatgatggac aggtcggaga ataggtgtgg aggcaatttc
     1381 ctgaagaggg ggagcatcat caacggggcg gactggtaca gcttcacggg aggcatgtcc
     1441 gatttcaact acctgcacac caactgcttt gagatcacgg tagagctggg ctgtgtgaag
     1501 ttcccccccg aggaggccct gtacatactc tggcagcaca acaaggagtc actcctgaat
     1561 ttcgtggaga cggtgcaccg gggcatcaaa ggtgtggtga cagataaatt cggcaagcca
     1621 gtcaaaaacg cccggatctc agtcaaaggc attcgccacg acatcaccac agccccagat
     1681 ggtgactact ggagactgct gcccccaggt atccacattg tcattgccca agcccctggc
     1741 tacgccaaag tcatcaagaa agtcatcatc cccgcccgga tgaagagggc tggccgtgtg
     1801 gacttcattc tgcaacctct ggggatggga cccaagaact ttattcatgg gctgcggagg
     1861 actgggcccc acgacccact gggaggtgcc agctctttgg gggaggccac ggagcccgac
     1921 ccgctccggg cgcgcaggca gccctcggcc gacgggagta agccctggtg gtggtcctac
     1981 ttcacatcgc tgagcaccca caggccacgc tggctgctca agtactagcc ccggccccag
     2041 cacccgccag gatgtggaga ccgaggccca tctccgcatc ccgggctcct ggctcttgat
     2101 tttgtctgcc acagacatcc cacaaagccg ctgccatttt attaaagtgt tttgatccac
     2161 ttt
//
