LOCUS       NM_007007               6584 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens cleavage and polyadenylation specific factor 6
            (CPSF6), transcript variant 1, mRNA.
ACCESSION   NM_007007
VERSION     NM_007007.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Khoroshkin,M., Buyan,A., Dodel,M., Navickas,A., Yu,J., Trejo,F.,
            Doty,A., Baratam,R., Zhou,S., Lee,S.B., Joshi,T., Garcia,K.,
            Choi,B., Miglani,S., Subramanyam,V., Modi,H., Carpenter,C.,
            Markett,D., Corces,M.R., Mardakheh,F.K., Kulakovskiy,I.V. and
            Goodarzi,H.
  TITLE     Systematic identification of post-transcriptional regulatory
            modules
  JOURNAL   Nat Commun 15 (1), 7872 (2024)
   PUBMED   39251607
  REMARK    Erratum:[Nat Commun. 2024 Oct 28;15(1):9277. doi:
            10.1038/s41467-024-52903-4.. PMID: 39468017]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6584)
  AUTHORS   Guedan,A., Burley,M., Caroe,E.R. and Bishop,K.N.
  TITLE     HIV-1 Capsid Rapidly Induces Long-Lived CPSF6 Puncta in
            Non-Dividing Cells, but Similar Puncta Already Exist in Uninfected
            T-Cells
  JOURNAL   Viruses 16 (5), 670 (2024)
   PUBMED   38793552
  REMARK    GeneRIF: HIV-1 Capsid Rapidly Induces Long-Lived CPSF6 Puncta in
            Non-Dividing Cells, but Similar Puncta Already Exist in Uninfected
            T-Cells.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6584)
  AUTHORS   Ge,Y., Huang,J., Chen,R., Fu,Y., Ling,T., Ou,X., Rong,X., Cheng,Y.,
            Lin,Y., Zhou,F., Lu,C., Yuan,S. and Xu,A.
  TITLE     Downregulation of CPSF6 leads to global mRNA 3' UTR shortening and
            enhanced antiviral immune responses
  JOURNAL   PLoS Pathog 20 (2), e1012061 (2024)
   PUBMED   38416782
  REMARK    GeneRIF: Downregulation of CPSF6 leads to global mRNA 3' UTR
            shortening and enhanced antiviral immune responses.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6584)
  AUTHORS   Liu,S., Wu,R., Chen,L., Deng,K., Ou,X., Lu,X., Li,M., Liu,C.,
            Chen,S., Fu,Y. and Xu,A.
  TITLE     CPSF6 regulates alternative polyadenylation and proliferation of
            cancer cells through phase separation
  JOURNAL   Cell Rep 42 (10), 113197 (2023)
   PUBMED   37777964
  REMARK    GeneRIF: CPSF6 regulates alternative polyadenylation and
            proliferation of cancer cells through phase separation.
REFERENCE   5  (bases 1 to 6584)
  AUTHORS   Luchsinger,C., Lee,K., Mardones,G.A., KewalRamani,V.N. and
            Diaz-Griffero,F.
  TITLE     Formation of nuclear CPSF6/CPSF5 biomolecular condensates upon
            HIV-1 entry into the nucleus is important for productive infection
  JOURNAL   Sci Rep 13 (1), 10974 (2023)
   PUBMED   37414787
  REMARK    GeneRIF: Formation of nuclear CPSF6/CPSF5 biomolecular condensates
            upon HIV-1 entry into the nucleus is important for productive
            infection.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6584)
  AUTHORS   Dettwiler,S., Aringhieri,C., Cardinale,S., Keller,W. and
            Barabino,S.M.
  TITLE     Distinct sequence motifs within the 68-kDa subunit of cleavage
            factor Im mediate RNA binding, protein-protein interactions, and
            subcellular localization
  JOURNAL   J Biol Chem 279 (34), 35788-35797 (2004)
   PUBMED   15169763
  REMARK    GeneRIF: region in the subunit of CF I(m) involved in RNA binding,
            protein-protein interactions, and subcellular localization
REFERENCE   7  (bases 1 to 6584)
  AUTHORS   Brown,K.M. and Gilmartin,G.M.
  TITLE     A mechanism for the regulation of pre-mRNA 3' processing by human
            cleavage factor Im
  JOURNAL   Mol Cell 12 (6), 1467-1476 (2003)
   PUBMED   14690600
  REMARK    GeneRIF: Data show that cleavage factor I(m) can stimulate both
            cleavage and poly(adenine) addition, and can act to suppress
            poly(A) site cleavage in a sequence-dependent manner.
            GeneRIF: Elevated levels of human cleavage factor Im suppressed
            cleavage at the primary poly(A) site of the pre-mRNA encoding the
            68 kDa subunit of CFI(m).
REFERENCE   8  (bases 1 to 6584)
  AUTHORS   Awasthi,S. and Alwine,J.C.
  TITLE     Association of polyadenylation cleavage factor I with U1 snRNP
  JOURNAL   RNA 9 (11), 1400-1409 (2003)
   PUBMED   14561889
REFERENCE   9  (bases 1 to 6584)
  AUTHORS   Ruegsegger,U., Blank,D. and Keller,W.
  TITLE     Human pre-mRNA cleavage factor Im is related to spliceosomal SR
            proteins and can be reconstituted in vitro from recombinant
            subunits
  JOURNAL   Mol Cell 1 (2), 243-253 (1998)
   PUBMED   9659921
REFERENCE   10 (bases 1 to 6584)
  AUTHORS   Ruegsegger,U., Beyer,K. and Keller,W.
  TITLE     Purification and characterization of human cleavage factor Im
            involved in the 3' end processing of messenger RNA precursors
  JOURNAL   J Biol Chem 271 (11), 6107-6113 (1996)
   PUBMED   8626397
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA365448.1, AK223568.1, AC020656.33 and AW189370.1.
            
            On Nov 22, 2018 this sequence version replaced NM_007007.2.
            
            Summary: The protein encoded by this gene is one subunit of a
            cleavage factor required for 3' RNA cleavage and polyadenylation
            processing. The interaction of the protein with the RNA is one of
            the earliest steps in the assembly of the 3' end processing complex
            and facilitates the recruitment of other processing factors. The
            cleavage factor complex is composed of four polypeptides. This gene
            encodes the 68kD subunit. It has a domain organization reminiscent
            of spliceosomal proteins. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) lacks an alternate in-frame
            exon in the central coding region, compared to variant 2. The
            encoded isoform (1) is shorter than isoform 2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            transcript and genomic sequence to make the sequence consistent
            with the reference genome assembly. The genomic coordinates used
            for the transcript record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.127421.1,
                                           SRR1660805.242393.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000435070.7/ ENSP00000391774.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 DA365448.1         33-35
            4-2116              AK223568.1         1-2113
            2117-6396           AC020656.33        38062-42341
            6397-6584           AW189370.1         15-202              c
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q15"
     gene            1..6584
                     /gene="CPSF6"
                     /gene_synonym="CFIM; CFIM68; CFIM72; HPBRII-4; HPBRII-7"
                     /note="cleavage and polyadenylation specific factor 6"
                     /db_xref="GeneID:11052"
                     /db_xref="HGNC:HGNC:13871"
                     /db_xref="MIM:604979"
     exon            1..138
                     /gene="CPSF6"
                     /gene_synonym="CFIM; CFIM68; CFIM72; HPBRII-4; HPBRII-7"
                     /inference="alignment:Splign:2.1.0"
     CDS             79..1734
                     /gene="CPSF6"
                     /gene_synonym="CFIM; CFIM68; CFIM72; HPBRII-4; HPBRII-7"
                     /note="isoform 1 is encoded by transcript variant 1;
                     pre-mRNA cleavage factor Im (68kD); pre-mRNA cleavage
                     factor I, 68kD subunit; cleavage factor Im complex 68 kDa
                     subunit; cleavage and polyadenylation specificity factor
                     subunit 6; protein HPBRII-4/7; CPSF 68 kDa subunit;
                     pre-mRNA cleavage factor Im 68 kDa subunit; cleavage and
                     polyadenylation specificity factor 68 kDa subunit;
                     cleavage and polyadenylation specific factor 6, 68kDa"
                     /codon_start=1
                     /product="cleavage and polyadenylation specificity factor
                     subunit 6 isoform 1"
                     /protein_id="NP_008938.2"
                     /db_xref="CCDS:CCDS8988.1"
                     /db_xref="GeneID:11052"
                     /db_xref="HGNC:HGNC:13871"
                     /db_xref="MIM:604979"
                     /translation="MADGVDHIDIYADVGEEFNQEAEYGGHDQIDLYDDVISPSANNG
                     DAPEDRDYMDTLPPTVGDDVGKGAAPNVVYTYTGKRIALYIGNLTWWTTDEDLTEAVH
                     SLGVNDILEIKFFENRANGQSKGFALVGVGSEASSKKLMDLLPKRELHGQNPVVTPCN
                     KQFLSQFEMQSRKTTQSGQMSGEGKAGPPGGSSRAAFPQGGRGRGRFPGAVPGGDRFP
                     GPAGPGGPPPPFPAGQTPPRPPLGPPGPPGPPGPPPPGQVLPPPLAGPPNRGDRPPPP
                     VLFPGQPFGQPPLGPLPPGPPPPVPGYGPPPGPPPPQQGPPPPPGPFPPRPPGPLGPP
                     LTLAPPPHLPGPPPGAPPPAPHVNPAFFPPPTNSGMPTSDSRGPPPTDPYGRPPPYDR
                     GDYGPPGREMDTARTPLSEAEFEEIMNRNRAISSSAISRAVSDASAGDYGSAIETLVT
                     AISLIKQSKVSADDRCKVLISSLQDCLHGIESKSYGSGSRRERSRERDHSRSREKSRR
                     HKSRSRDRHDDYYRERSRERERHRDRDRDRDRERDREREYRHR"
     exon            139..348
                     /gene="CPSF6"
                     /gene_synonym="CFIM; CFIM68; CFIM72; HPBRII-4; HPBRII-7"
                     /inference="alignment:Splign:2.1.0"
     exon            349..452
                     /gene="CPSF6"
                     /gene_synonym="CFIM; CFIM68; CFIM72; HPBRII-4; HPBRII-7"
                     /inference="alignment:Splign:2.1.0"
     exon            453..598
                     /gene="CPSF6"
                     /gene_synonym="CFIM; CFIM68; CFIM72; HPBRII-4; HPBRII-7"
                     /inference="alignment:Splign:2.1.0"
     exon            599..772
                     /gene="CPSF6"
                     /gene_synonym="CFIM; CFIM68; CFIM72; HPBRII-4; HPBRII-7"
                     /inference="alignment:Splign:2.1.0"
     exon            773..1277
                     /gene="CPSF6"
                     /gene_synonym="CFIM; CFIM68; CFIM72; HPBRII-4; HPBRII-7"
                     /inference="alignment:Splign:2.1.0"
     exon            1278..1393
                     /gene="CPSF6"
                     /gene_synonym="CFIM; CFIM68; CFIM72; HPBRII-4; HPBRII-7"
                     /inference="alignment:Splign:2.1.0"
     exon            1394..1547
                     /gene="CPSF6"
                     /gene_synonym="CFIM; CFIM68; CFIM72; HPBRII-4; HPBRII-7"
                     /inference="alignment:Splign:2.1.0"
     exon            1548..1737
                     /gene="CPSF6"
                     /gene_synonym="CFIM; CFIM68; CFIM72; HPBRII-4; HPBRII-7"
                     /inference="alignment:Splign:2.1.0"
     exon            1738..6584
                     /gene="CPSF6"
                     /gene_synonym="CFIM; CFIM68; CFIM72; HPBRII-4; HPBRII-7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2147..2152
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CPSF6"
                     /gene_synonym="CFIM; CFIM68; CFIM72; HPBRII-4; HPBRII-7"
                     /note="hexamer: AATAAA"
     polyA_site      2165
                     /gene="CPSF6"
                     /gene_synonym="CFIM; CFIM68; CFIM72; HPBRII-4; HPBRII-7"
                     /note="major polyA site"
     regulatory      6562..6567
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CPSF6"
                     /gene_synonym="CFIM; CFIM68; CFIM72; HPBRII-4; HPBRII-7"
                     /note="hexamer: AATAAA"
     polyA_site      6584
                     /gene="CPSF6"
                     /gene_synonym="CFIM; CFIM68; CFIM72; HPBRII-4; HPBRII-7"
ORIGIN      
        1 gctagatccg ctgctgctgc cgcggcgggc agacctgcag gaggcggcgg cggcggcggc
       61 ggccgaggct gaaggaagat ggcggacggc gtggaccaca tagacattta cgcggatgtc
      121 ggcgaagagt tcaaccagga agctgaatat ggtgggcatg atcagataga tttgtatgac
      181 gatgtcatat ctccatctgc aaataatgga gatgccccag aagaccgaga ttacatggat
      241 actctcccac caactgttgg tgatgatgtg ggtaaaggag cagcaccaaa tgttgtctat
      301 acatatactg gaaagagaat tgcattatat attggaaatc taacatggtg gacaacagat
      361 gaagacttaa ctgaagcagt tcattctttg ggagtaaatg atattttgga gataaaattt
      421 tttgaaaatc gggcaaatgg ccagtcaaag gggtttgccc ttgttggtgt tggatctgaa
      481 gcatcttcaa aaaagttaat ggatctgtta cctaaaagag aacttcatgg tcagaatcct
      541 gttgtaactc catgcaataa acagttcctg agtcaatttg aaatgcagtc caggaaaact
      601 acacaatcag gacaaatgtc tggggaaggt aaagctggtc ctccaggagg cagttcccgt
      661 gcagcatttc cacaaggtgg tagaggacgg ggccgttttc caggggctgt tcctggtggg
      721 gacagatttc ctgggccagc aggaccagga gggccacccc caccttttcc agctggacag
      781 actccaccac gtccaccctt aggtcctcca ggcccacctg gtccaccagg tcctccacct
      841 cctggtcagg ttctgcctcc tcctctagct gggcctccta atcgaggaga tcgccctcca
      901 ccaccagttc tttttcctgg acaacctttt gggcagcctc cattgggtcc acttcctcct
      961 ggccctccac ctccagttcc aggctacggc ccccctcctg gcccaccacc tccacaacag
     1021 ggaccacctc cacctccagg cccctttcca cctcgtccac ccggtccact tgggccaccc
     1081 cttacactag ctcctcctcc gcatcttcct ggaccacctc caggtgcccc accgccagct
     1141 ccgcatgtga acccagcttt ctttcctcca ccaactaaca gtggcatgcc tacatcagat
     1201 agccgaggtc caccaccaac agatccatat gggcgacctc caccatatga taggggtgac
     1261 tatggccccc ctggaaggga aatggatact gcaagaacgc cattgagtga agctgaattt
     1321 gaagaaatca tgaatagaaa tagggcaatc tcaagcagtg ctatttcgag agctgtgtct
     1381 gatgccagtg ctggtgatta tgggagtgct attgagacac tggtaactgc aatttcttta
     1441 attaaacaat ccaaagtatc tgctgatgat cgttgcaaag ttcttattag ttctttgcaa
     1501 gattgccttc atggaattga gtccaagtct tatggttctg gatcaagacg tgaacgatca
     1561 agagagaggg accatagtag atcacgagaa aagagtcgac gtcataaatc ccgtagtaga
     1621 gaccgtcatg acgattatta cagagagaga agcagagaac gagagaggca ccgggatcgt
     1681 gaccgagacc gtgaccgaga gcgtgaccga gagcgcgaat atcgtcatcg ttagaagctg
     1741 aaggaagagg atcaccttcc aagacaaaac agtcttcatg ggggaaaaat gacgcttgtc
     1801 cagcagtttg cttcttgtga ttgaactgaa cctgtaagga ttcatggata aaatgaacag
     1861 gaatagatct gaataaagca aatctgcata aatggtaacc agtagctcta cttttatttt
     1921 ttatgttgct taactgtttt atttgaagga aacctgtgtg atttaaaaag ttatagcttt
     1981 tgcaacttta ttactggtta tatacatttg gccattatga tgtgcaagca attggaaaaa
     2041 aagtcaagta aatgcttgtt tttgtagtag tttgttcttg ttaaaaatgt ttatatgata
     2101 atgtctgtaa acagcatcac tttgattaca atagatgtag tgttgtaata aactgtttaa
     2161 tggggctgat gtgtaaagct gttcaagtta tttgatgttt acacctcagg gaaagtcttg
     2221 tgttcagcaa tatctaaaga taatgttact atgacaacat ttttactgtc ctttaaagca
     2281 ttgcaatagc gtttttggat atgcctcaat ctaatcttgc gttcagtgaa ttaaacatag
     2341 taattaagtg tcttttgccc ttgattttga tattagaata ggtgattaca tggatattta
     2401 atatttctat attctgcttt tctagctgtt tttacctagt tagcttgtga ctttgctgaa
     2461 tggtatgtaa acttgtaaaa atagagattt gacagacata gcaatctagt caatgtgtaa
     2521 ggggtcaaaa aaacagaggt tttaacacat aagtaaaaac ccgtacatat ttgatgtgta
     2581 atgcaggtta attacaacac agatgtaccg aaacacttaa ttgtgaaccg ctaacattga
     2641 agaaattttg acaattccga tttgatgctg caattacttg ctgtttttat tgatcttatg
     2701 gtttatttct taagccatag tcagtgtaaa tacagccctg cagcaggtaa atgtgagtaa
     2761 agagagcctt atattttcca attggtataa atttttgaag gatgtgatgt tcattaacat
     2821 tcggttgtat tccccagtat ttgtaatggg aaattacaga taaaccgtgt ctgcacagtt
     2881 taaggaatac tatgtatatt catgcaccgt attgattcat gctatagtta cttaatcaaa
     2941 gatttttttc aaacctgcct tacatatagg cccactttaa aagcacctga ctagcatgtg
     3001 ttcttgattg caaaattggc agaggcaggg tgtcaacttg attaggtgtt tttatgggaa
     3061 tgtaatttga aatcactact tcagaaattt gacttaaaat tcttgagcac gttaatatgt
     3121 ttttaagatc tgattatctt tgagagatct tctgttaata cacattggtt gttaaagagt
     3181 acccaaattc taggacaatg cttaaagtgt taaaataccc tagatactgt gttatgtgca
     3241 actgtagaaa ccctccagaa atttccactg ctgttcttca ctttcatctt gtctgctatc
     3301 aaaccacttc tgacaaaatt agctgttttg aattacccat atcactgcca gttttatttt
     3361 aaaatatttt gtgtttgaag tatctgtgca tgggatcgtt gatgtttatc agaactgttc
     3421 actttcagaa atgatttttt aaagcatttt gttgaaaatg cggttgcttt tttaacatta
     3481 ttttagagtt gtatcaaaat tttcctgttt tctgtatatt gttgtgtcat ttcttggctt
     3541 tttaatattt tgactacaat gccctaatgc gaagaagtat ggacatataa atattacaag
     3601 agaaattagt taatagtaaa gggttaaaag gtaagtaaaa tgtccatgaa aataaaattt
     3661 tctttgaaca gtttaaaaat ggaatcacaa aaatctaact gcacttgaaa tgaaactgga
     3721 aattttatac taattaaatc ctttatttac cttttcatat tttatcaatg gaacctttta
     3781 attgctcctt ctatatcact cttctccttg cttggctgat ctcattacat gactttcgtt
     3841 acagacagtg tgagtgtact agatttgttt gctgctaatc cgttatttta agtgttgtca
     3901 gaaggaaata acttcttggt ttgaccaagt aattttgcaa agtccttctc tacctgataa
     3961 cacgtgaatg tatagcagta aaggtgaaaa ttgcaggcct tgttttgttc ttcatacccc
     4021 cttcagttgt ttatgggtta atgttatttg aaaactcttc ctttttttaa tgtgtaaaaa
     4081 caatccaaag agataaatcc ttgcatagac accaaaaaca gtgtctcaga gaccttttca
     4141 ttttttcctt agttaataac tgtagtggat gtgaaaacaa acgagttgaa ttttaaaaca
     4201 tagcatttat tagtgctata cagtcacatt cctttcagcc agtagtaaag ttaaacagat
     4261 gagtgataca gtacctgaat ggtactgctt ctgaggcaac actttggatt gaaagtgctg
     4321 cttttggtta aaggaactat gttgtggaaa gcatattcca gtgtaaattt gcagatgtgt
     4381 gttttttttt tttgtcactt ttcataaggt ttggagtaag ttgcagtggc ttttgatcat
     4441 agtttagccc atttaatggt ccttttacct agaatgttct taagcagtta atgacaattt
     4501 ggaatacaca gataacttgt agcttaagat actattttca tttaattctc ctgtggagaa
     4561 tattgcttta ttttttacta gtttcaggtc agttattaaa ataaacatgg aggttttaat
     4621 gaatgttgta taatatttta tagtgaagga aaattgtgga aagggcaaag actttctctt
     4681 tcccttatgt atttaactgt gccaatctaa atacatactg ttttatacaa tgagatgcat
     4741 tacaagactt ttctgttgga gtgctctcga cattttggtt ttcatacctg aatgatcatt
     4801 gcattttctg tattttctaa aatggctcca attttgtgtt ttaagcttca gcttaagagg
     4861 aagtttatgt tctaattctt gactgagaat acagtattga gattctctgt tttacagata
     4921 acaactggtt tttattactc attaagttca tttgcatccc gtagccctct gtaaatgttt
     4981 cccctagttg tatgtacgta aatgcacgct tatccagtgt atattagaca tttttgtgct
     5041 aaaatatatt aagtgggatt tttgtagcaa agcattctat ttttctgttc ttacagtgtg
     5101 tgtgtgtgtg tgtgtgtgtg tgtatgcgtt tttttaacct taacttcagt ttaaacactg
     5161 gtgtatttat ttttttaagc aacttgactc ttagaagcct tagactgatt ttttcaataa
     5221 atattcaacc aaaaattata aatttattaa gatgtggcct tacatatggc attccttgtg
     5281 ttcgtaatgt gagatttttg atttagataa atcaagattc aggattaaag tttcattgta
     5341 agttgaaata gaaaatgtat taaaatgtct aggcttctgg gaggaagttc ttatactctt
     5401 ctttcttggc attagaaaga agcaatatga atttttgtga atattctaaa tattcaggca
     5461 acactgttca gattgattta ggtttgtctt aaccaatgtt ctttttttag aatttcaggt
     5521 tgtggcattc actgagtatg cagctactat ggtttttgta tgggacgtat aaatacttga
     5581 ttatatacga cagattttaa tgtctttaaa gacttcctgc tgtattaaca tattgtaatg
     5641 gagtctttta aatactaggt tgaatttaat tgaagtcaca cacatcttga agtggtaact
     5701 gcatagtaaa tactaccaag agtttttttc acgtgggagt atcctaaaac tctgccatgg
     5761 gtgtaaatgt tttacattaa tttcataatt ggacagaccc tgcatttagc gaaaacattt
     5821 tgttttgaaa gtgtgttctt tttgtcgcac tgttactgcg taacacttct caacattctg
     5881 taagttaaat tattttaaaa taactatggt gaattcatgt ttattttttt actttgaaaa
     5941 ttgtagtact caggtggtat ttaatgggaa aggatccttt gggtataaat cataatgtat
     6001 tttaaggaat gcatctataa cattgataac tttagcctta aaaaaaaggt ctattattcc
     6061 ttcctctgca tcccatggta tattagcaaa gtagtttaca gccctgtaca gccttacaaa
     6121 gttgttttac aatttttaat ggaaagccct taacaacaac aacaaaaaat tgtatcattt
     6181 taccaatatc cacgaattac tgatcacagt ttgtaaataa tttgtttaat atcagaaggc
     6241 caaagaaagt cttaaaattt tagctattga ctctatggtg acttcacaat aagatttctg
     6301 tgcaaaaagg taaggtgata attgatctaa tagacttttt tttttttttt tttgctgtcc
     6361 atgagaatta gacttcatat tagcttcaat taaaaagctc attccatttt attaaaatgt
     6421 ttgtaattgc aattttgtgt ttaatgttta ctttgtcttt cctttcttta gcatttaggt
     6481 gactgttcgg cagtttgata tatggccatt gtcggcctta aactgcacta gtaacaagca
     6541 tggtatttat cttgtattga aaataaaaca aacttttgat aaga
//
