LOCUS       NM_006693               1823 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens cleavage and polyadenylation specific factor 4
            (CPSF4), transcript variant 1, mRNA.
ACCESSION   NM_006693
VERSION     NM_006693.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Choudhry,M., Gamallat,Y., Ghosh,S. and Bismar,T.A.
  TITLE     Cleavage and Polyadenylation-Specific Factor 4 (CPSF4) Expression
            Is Associated with Enhanced Prostate Cancer Cell Migration and Cell
            Cycle Dysregulation, In Vitro
  JOURNAL   Int J Mol Sci 24 (16), 12961 (2023)
   PUBMED   37629142
  REMARK    GeneRIF: Cleavage and Polyadenylation-Specific Factor 4 (CPSF4)
            Expression Is Associated with Enhanced Prostate Cancer Cell
            Migration and Cell Cycle Dysregulation, In Vitro.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Song,Y., Sun,K., Gong,L., Shi,L., Qin,T., Wang,S., Deng,W.,
            Chen,W., Zheng,F. and Li,G.
  TITLE     CPSF4 promotes tumor-initiating phenotype by enhancing
            VEGF/NRP2/TAZ signaling in lung cancer
  JOURNAL   Med Oncol 40 (1), 62 (2022)
   PUBMED   36567417
  REMARK    GeneRIF: CPSF4 promotes tumor-initiating phenotype by enhancing
            VEGF/NRP2/TAZ signaling in lung cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1823)
  AUTHORS   Wang,X., Dong,J., Li,X., Cheng,Z. and Zhu,Q.
  TITLE     CPSF4 regulates circRNA formation and microRNA mediated gene
            silencing in hepatocellular carcinoma
  JOURNAL   Oncogene 40 (25), 4338-4351 (2021)
   PUBMED   34103682
  REMARK    GeneRIF: CPSF4 regulates circRNA formation and microRNA mediated
            gene silencing in hepatocellular carcinoma.
REFERENCE   4  (bases 1 to 1823)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 1823)
  AUTHORS   Lee,K., Zheng,Q., Lu,Q., Xu,F., Qin,G., Zhai,Q., Hong,R., Chen,M.,
            Deng,W. and Wang,S.
  TITLE     CPSF4 promotes triple negative breast cancer metastasis by
            upregulating MDM4
  JOURNAL   Signal Transduct Target Ther 6 (1), 184 (2021)
   PUBMED   34006850
  REMARK    GeneRIF: CPSF4 promotes triple negative breast cancer metastasis by
            upregulating MDM4.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1823)
  AUTHORS   Nemeroff,M.E., Barabino,S.M., Li,Y., Keller,W. and Krug,R.M.
  TITLE     Influenza virus NS1 protein interacts with the cellular 30 kDa
            subunit of CPSF and inhibits 3'end formation of cellular pre-mRNAs
  JOURNAL   Mol Cell 1 (7), 991-1000 (1998)
   PUBMED   9651582
REFERENCE   7  (bases 1 to 1823)
  AUTHORS   Barabino,S.M., Hubner,W., Jenny,A., Minvielle-Sebastia,L. and
            Keller,W.
  TITLE     The 30-kD subunit of mammalian cleavage and polyadenylation
            specificity factor and its yeast homolog are RNA-binding zinc
            finger proteins
  JOURNAL   Genes Dev 11 (13), 1703-1716 (1997)
   PUBMED   9224719
REFERENCE   8  (bases 1 to 1823)
  AUTHORS   McCracken,S., Fong,N., Yankulov,K., Ballantyne,S., Pan,G.,
            Greenblatt,J., Patterson,S.D., Wickens,M. and Bentley,D.L.
  TITLE     The C-terminal domain of RNA polymerase II couples mRNA processing
            to transcription
  JOURNAL   Nature 385 (6614), 357-361 (1997)
   PUBMED   9002523
REFERENCE   9  (bases 1 to 1823)
  AUTHORS   Jenny,A., Hauri,H.P. and Keller,W.
  TITLE     Characterization of cleavage and polyadenylation specificity factor
            and cloning of its 100-kilodalton subunit
  JOURNAL   Mol Cell Biol 14 (12), 8183-8190 (1994)
   PUBMED   7969155
REFERENCE   10 (bases 1 to 1823)
  AUTHORS   Thuresson,A.C., Astrom,J., Astrom,A., Gronvik,K.O. and Virtanen,A.
  TITLE     Multiple forms of poly(A) polymerases in human cells
  JOURNAL   Proc Natl Acad Sci U S A 91 (3), 979-983 (1994)
   PUBMED   8302877
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BM757197.1, CX754488.1, U79569.1, BQ641871.1 and AA040394.1.
            
            On Nov 23, 2018 this sequence version replaced NM_006693.3.
            
            Summary: Inhibition of the nuclear export of poly(A)-containing
            mRNAs caused by the influenza A virus NS1 protein requires its
            effector domain. The NS1 effector domain functionally interacts
            with the cellular 30 kDa subunit of cleavage and polyadenylation
            specific factor 4, an essential component of the 3' end processing
            machinery of cellular pre-mRNAs. In influenza virus-infected cells,
            the NS1 protein is physically associated with cleavage and
            polyadenylation specific factor 4, 30kD subunit. Binding of the NS1
            protein to the 30 kDa protein in vitro prevents CPSF binding to the
            RNA substrate and inhibits 3' end cleavage and polyadenylation of
            host pre-mRNAs. Thus the NS1 protein selectively inhibits the
            nuclear export of cellular, and not viral, mRNAs. Multiple
            alternatively spliced transcript variants that encode different
            isoforms have been described for this gene. [provided by RefSeq,
            Jul 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.572746.1,
                                           SRR3476690.455711.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000292476.10/ ENSP00000292476.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-447               BM757197.1         4-450
            448-710             CX754488.1         419-681
            711-1099            U79569.1           607-995
            1100-1555           BQ641871.1         191-646
            1556-1823           AA040394.1         4-271               c
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q22.1"
     gene            1..1823
                     /gene="CPSF4"
                     /gene_synonym="CPSF30; NAR; NEB-1; NEB1"
                     /note="cleavage and polyadenylation specific factor 4"
                     /db_xref="GeneID:10898"
                     /db_xref="HGNC:HGNC:2327"
                     /db_xref="MIM:603052"
     exon            1..243
                     /gene="CPSF4"
                     /gene_synonym="CPSF30; NAR; NEB-1; NEB1"
                     /inference="alignment:Splign:2.1.0"
     CDS             141..950
                     /gene="CPSF4"
                     /gene_synonym="CPSF30; NAR; NEB-1; NEB1"
                     /note="isoform 1 is encoded by transcript variant 1; no
                     arches-like zinc finger protein; cleavage and
                     polyadenylation specificity factor subunit 4; no arches
                     homolog; CPSF 30 kDa subunit; cleavage and polyadenylation
                     specific factor 4, 30kDa; NS1 effector domain-binding
                     protein 1; cleavage and polyadenylation specificity factor
                     30 kDa subunit"
                     /codon_start=1
                     /product="cleavage and polyadenylation specificity factor
                     subunit 4 isoform 1"
                     /protein_id="NP_006684.1"
                     /db_xref="CCDS:CCDS5664.1"
                     /db_xref="GeneID:10898"
                     /db_xref="HGNC:HGNC:2327"
                     /db_xref="MIM:603052"
                     /translation="MQEIIASVDHIKFDLEIAVEQQLGAQPLPFPGMDKSGAAVCEFF
                     LKAACGKGGMCPFRHISGEKTVVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFYSKF
                     GECSNKECPFLHIDPESKIKDCPWYDRGFCKHGPLCRHRHTRRVICVNYLVGFCPEGP
                     SCKFMHPRFELPMGTTEQPPLPQQTQPPAKQSNNPPLQRSSSLIQLTSQNSSPNQQRT
                     PQVIGVMQSQNSSAGNRGPRPLEQVTCYKCGEKGHYANRCTKGHLAFLSGQ"
     exon            244..294
                     /gene="CPSF4"
                     /gene_synonym="CPSF30; NAR; NEB-1; NEB1"
                     /inference="alignment:Splign:2.1.0"
     exon            295..447
                     /gene="CPSF4"
                     /gene_synonym="CPSF30; NAR; NEB-1; NEB1"
                     /inference="alignment:Splign:2.1.0"
     exon            448..543
                     /gene="CPSF4"
                     /gene_synonym="CPSF30; NAR; NEB-1; NEB1"
                     /inference="alignment:Splign:2.1.0"
     exon            544..637
                     /gene="CPSF4"
                     /gene_synonym="CPSF30; NAR; NEB-1; NEB1"
                     /inference="alignment:Splign:2.1.0"
     exon            638..710
                     /gene="CPSF4"
                     /gene_synonym="CPSF30; NAR; NEB-1; NEB1"
                     /inference="alignment:Splign:2.1.0"
     exon            711..881
                     /gene="CPSF4"
                     /gene_synonym="CPSF30; NAR; NEB-1; NEB1"
                     /inference="alignment:Splign:2.1.0"
     exon            882..1823
                     /gene="CPSF4"
                     /gene_synonym="CPSF30; NAR; NEB-1; NEB1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1805..1810
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CPSF4"
                     /gene_synonym="CPSF30; NAR; NEB-1; NEB1"
                     /note="hexamer: ATTAAA"
     polyA_site      1823
                     /gene="CPSF4"
                     /gene_synonym="CPSF30; NAR; NEB-1; NEB1"
                     /note="major polyA site"
ORIGIN      
        1 ggcatccctc gggcggcggc ggcggcggcg gcgaggcgaa gcgaaggagg agtgtgtgcg
       61 gcggggccgg cggcgggtaa aggcgagaag gctgcaggag accgaggggg agccgggccg
      121 gtggggccgc cgccgccgcc atgcaggaaa tcatcgccag cgtggaccac atcaagtttg
      181 acttggagat cgcggtggag cagcagctgg gggcgcagcc gctgcccttc cccggcatgg
      241 acaagtcggg cgctgctgtc tgtgaattct ttttgaaagc tgcctgcggc aaagggggca
      301 tgtgtccgtt tcgccacatc agtggtgaga agacagttgt gtgcaaacac tggctgcgtg
      361 gcctatgcaa gaaaggggac cagtgtgagt tcctgcatga gtatgacatg accaagatgc
      421 ccgagtgcta cttctactcc aagttcgggg agtgcagcaa caaggaatgt cccttcctgc
      481 acatcgaccc cgagtccaag atcaaggact gtccttggta tgaccgtggc ttctgcaagc
      541 acggtcccct ctgcaggcac cggcacacac ggagagtcat ctgtgtgaat tacctcgtgg
      601 gattctgccc ggaggggccc tcgtgtaaat tcatgcaccc tcgatttgaa ctgcccatgg
      661 gaaccaccga gcagccccca ctgccgcagc agacacagcc tccagcaaag caaagtaaca
      721 atccgccatt acaaaggtcg tcctccttga tccagttaac gagtcagaac tcttctccca
      781 atcagcagag aaccccgcag gtcatcgggg tcatgcagag tcaaaacagc agcgcgggca
      841 accggggacc ccggccactg gagcaggtca cctgttacaa gtgtggcgag aaaggacact
      901 acgccaacag atgcaccaaa gggcacttgg cctttctcag tggacagtga cagcagctgg
      961 agccagctcc gagcagcccg ggggccccgc tgttgggagt gtgcatttaa ctgtttcatg
     1021 cgcttgttgg cgcgactgtg gctcgagctg gcccgcagac acgtgggttt catcactctg
     1081 aggggccacg tctgttagtt tcctatcatt ttgccttagt attttttgaa aaagggacat
     1141 gtgtcctgtg ggtccctgca gtcgacatca tgtttggctg ggcatcgatg cctcctttct
     1201 gggactcccg gcacaactcc cctcatccag ggagggaggc agctgctggg gaggggcttg
     1261 gctaggtagt tctgtgtggc ggtggtcatt cccctcatta aacaccagtt cttggtgacg
     1321 ccaggggctg gtaggtcatt caaagctgtg gccagctcac gcctgcttcc tccctccctg
     1381 ccctgctgaa tcctaaagct gtgcctatat ctgtgatttg aatgagggag ccctttgggg
     1441 caaattcagg tgcccccatt gcctcaggct ggccctggtc ccaggtggca gcggttgagg
     1501 aggggtacag ggctctcaag cctgaggttt tcttctctgg gcttaatttt ctcttggggt
     1561 acgtgcctga cagtgtttaa ggtgtccgtt gaactggagt tgcagacttt taaatagatg
     1621 accccttcag atcatctgtg cctacctcct gcccatcagg cgtctacact gtcactcaga
     1681 cacctgtggc atgtggagga gactgccctg tcctgagcct ggaaaatgtg aaactgtctc
     1741 ctgcaacctg ctgggcatgt gggcctggct gtgttcaatt gcaagaacaa tttttatgaa
     1801 atggattaaa gcttgttttt taa
//
