LOCUS       NP_057291                684 aa            linear   PRI 24-JUN-2024
DEFINITION  cleavage and polyadenylation specificity factor subunit 3 isoform a
            [Homo sapiens].
ACCESSION   NP_057291
VERSION     NP_057291.1
DBSOURCE    REFSEQ: accession NM_016207.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 684)
  AUTHORS   Zhang,Y., Liu,D., Guo,D., Lin,W., Lu,W., Hu,L., Chen,S. and Chen,C.
  TITLE     CPSF3 regulates alternative polyadenylation of CNIH2 to promote
            esophageal squamous cell carcinoma progression
  JOURNAL   Cancer Lett 593, 216925 (2024)
   PUBMED   38718887
  REMARK    GeneRIF: CPSF3 regulates alternative polyadenylation of CNIH2 to
            promote esophageal squamous cell carcinoma progression.
REFERENCE   2  (residues 1 to 684)
  AUTHORS   Alahmari,A.A., Chaubey,A.H., Jonnakuti,V.S., Tisdale,A.A.,
            Schwarz,C.D., Cornwell,A.C., Maraszek,K.E., Paterson,E.J., Kim,M.,
            Venkat,S., Gomez,E.C., Wang,J., Gurova,K.V., Yalamanchili,H.K. and
            Feigin,M.E.
  TITLE     CPSF3 inhibition blocks pancreatic cancer cell proliferation
            through disruption of core histone mRNA processing
  JOURNAL   RNA 30 (3), 281-297 (2024)
   PUBMED   38191171
  REMARK    GeneRIF: CPSF3 inhibition blocks pancreatic cancer cell
            proliferation through disruption of core histone mRNA processing.
            Publication Status: Online-Only
REFERENCE   3  (residues 1 to 684)
  AUTHORS   Shen,P., Ye,K., Xiang,H., Zhang,Z., He,Q., Zhang,X., Cai,M.C.,
            Chen,J., Sun,Y., Lin,L., Qi,C., Zhang,M., Cheung,L.W.T., Shi,T.,
            Yin,X., Li,Y., Di,W., Zang,R., Tan,L. and Zhuang,G.
  TITLE     Therapeutic targeting of CPSF3-dependent transcriptional
            termination in ovarian cancer
  JOURNAL   Sci Adv 9 (47), eadj0123 (2023)
   PUBMED   37992178
  REMARK    GeneRIF: Therapeutic targeting of CPSF3-dependent transcriptional
            termination in ovarian cancer.
REFERENCE   4  (residues 1 to 684)
  AUTHORS   Huang,J., Liu,X., Sun,Y., Li,Z., Lin,M.H., Hamilton,K.,
            Mandel,C.R., Sandmeir,F., Conti,E., Oyala,P.H. and Tong,L.
  TITLE     An examination of the metal ion content in the active sites of
            human endonucleases CPSF73 and INTS11
  JOURNAL   J Biol Chem 299 (4), 103047 (2023)
   PUBMED   36822327
  REMARK    GeneRIF: An examination of the metal ion content in the active
            sites of human endonucleases CPSF73 and INTS11.
REFERENCE   5  (residues 1 to 684)
  AUTHORS   Arnadottir,G.A., Oddsson,A., Jensson,B.O., Gisladottir,S.,
            Simon,M.T., Arnthorsson,A.O., Katrinardottir,H., Fridriksdottir,R.,
            Ivarsdottir,E.V., Jonasdottir,A., Jonasdottir,A., Barrick,R.,
            Saemundsdottir,J., le Roux,L., Oskarsson,G.R., Asmundsson,J.,
            Steffensen,T., Gudmundsson,K.R., Ludvigsson,P., Jonsson,J.J.,
            Masson,G., Jonsdottir,I., Holm,H., Jonasson,J.G., Magnusson,O.T.,
            Thorarensen,O., Abdenur,J., Norddahl,G.L., Gudbjartsson,D.F.,
            Bjornsson,H.T., Thorsteinsdottir,U., Sulem,P. and Stefansson,K.
  TITLE     Population-level deficit of homozygosity unveils CPSF3 as an
            intellectual disability syndrome gene
  JOURNAL   Nat Commun 13 (1), 705 (2022)
   PUBMED   35121750
  REMARK    GeneRIF: Population-level deficit of homozygosity unveils CPSF3 as
            an intellectual disability syndrome gene.
            Publication Status: Online-Only
REFERENCE   6  (residues 1 to 684)
  AUTHORS   McCracken,S., Fong,N., Yankulov,K., Ballantyne,S., Pan,G.,
            Greenblatt,J., Patterson,S.D., Wickens,M. and Bentley,D.L.
  TITLE     The C-terminal domain of RNA polymerase II couples mRNA processing
            to transcription
  JOURNAL   Nature 385 (6614), 357-361 (1997)
   PUBMED   9002523
REFERENCE   7  (residues 1 to 684)
  AUTHORS   Jenny,A., Minvielle-Sebastia,L., Preker,P.J. and Keller,W.
  TITLE     Sequence similarity between the 73-kilodalton protein of mammalian
            CPSF and a subunit of yeast polyadenylation factor I
  JOURNAL   Science 274 (5292), 1514-1517 (1996)
   PUBMED   8929409
REFERENCE   8  (residues 1 to 684)
  AUTHORS   Murthy,K.G. and Manley,J.L.
  TITLE     The 160-kD subunit of human cleavage-polyadenylation specificity
            factor coordinates pre-mRNA 3'-end formation
  JOURNAL   Genes Dev 9 (21), 2672-2683 (1995)
   PUBMED   7590244
REFERENCE   9  (residues 1 to 684)
  AUTHORS   Jenny,A., Hauri,H.P. and Keller,W.
  TITLE     Characterization of cleavage and polyadenylation specificity factor
            and cloning of its 100-kilodalton subunit
  JOURNAL   Mol Cell Biol 14 (12), 8183-8190 (1994)
   PUBMED   7969155
REFERENCE   10 (residues 1 to 684)
  AUTHORS   Thuresson,A.C., Astrom,J., Astrom,A., Gronvik,K.O. and Virtanen,A.
  TITLE     Multiple forms of poly(A) polymerases in human cells
  JOURNAL   Proc Natl Acad Sci U S A 91 (3), 979-983 (1994)
   PUBMED   8302877
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC420903.1, BC020211.1 and
            AA635764.1.
            
            Summary: This gene encodes a member of the metallo-beta-lactamase
            family. The encoded protein is a 73kDa subunit of the cleavage and
            polyadenylation specificity factor and functions as an endonuclease
            that recognizes the pre-mRNA 3'-cleavage site AAUAAA prior to
            polyadenylation. It also cleaves after the pre-mRNA sequence ACCCA
            during histone 3'-end pre-mRNA processing. [provided by RefSeq, Oct
            2012].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.92137.1,
                                           SRR1803615.243113.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000238112.8/ ENSP00000238112.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p25.1"
     Protein         1..684
                     /product="cleavage and polyadenylation specificity factor
                     subunit 3 isoform a"
                     /note="cleavage and polyadenylation specificity factor
                     subunit 3; mRNA 3'-end-processing endonuclease CPSF-73;
                     cleavage and polyadenylation specific factor 3, 73kDa"
                     /calculated_mol_wt=77355
     Site            2
                     /site_type="acetylation"
                     /note="N-acetylserine.
                     /evidence=ECO:0007744|PubMed:19413330,
                     ECO:0007744|PubMed:22223895; propagated from
                     UniProtKB/Swiss-Prot (Q9UKF6.1)"
     Region          12..205
                     /region_name="CPSF3-like_MBL-fold"
                     /note="cleavage and polyadenylation specificity factor
                     (CPSF) subunit 3 and related proteins; MBL-fold
                     metallo-hydrolase domain; cd16292"
                     /db_xref="CDD:293850"
     Region          14..421
                     /region_name="YSH1"
                     /note="RNA processing exonuclease, beta-lactamase fold,
                     Cft2 family [Translation, ribosomal structure and
                     biogenesis]; COG1236"
                     /db_xref="CDD:440849"
     Site            order(71,73,75..76,158,179)
                     /site_type="active"
                     /db_xref="CDD:293850"
     Region          479..682
                     /region_name="CPSF73-100_C"
                     /note="Pre-mRNA 3'-end-processing endonuclease
                     polyadenylation factor C-term; pfam11718"
                     /db_xref="CDD:463330"
     Site            659
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9QXK7; propagated from
                     UniProtKB/Swiss-Prot (Q9UKF6.1)"
     Site            681
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0007744|PubMed:20068231,
                     ECO:0007744|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (Q9UKF6.1)"
     CDS             1..684
                     /gene="CPSF3"
                     /gene_synonym="CPSF-73; CPSF73; NEDMHS; NEDMHSN"
                     /coded_by="NM_016207.4:121..2175"
                     /note="isoform a is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS1664.1"
                     /db_xref="GeneID:51692"
                     /db_xref="HGNC:HGNC:2326"
                     /db_xref="MIM:606029"
ORIGIN      
        1 msaipaeesd qllirplgag qevgrsciil efkgrkimld cgihpglegm dalpyidlid
       61 paeidlllis hfhldhcgal pwflqktsfk grtfmthatk aiyrwllsdy vkvsnisadd
      121 mlytetdlee smdkietinf hevkevagik fwcyhaghvl gaamfmieia gvkllytgdf
      181 srqedrhlma aeipnikpdi liiestygth ihekreerea rfcntvhdiv nrggrglipv
      241 falgraqell lildeywqnh pelhdipiyy asslakkcma vyqtyvnamn dkirkqinin
      301 npfvfkhisn lksmdhfddi gpsvvmaspg mmqsglsrel feswctdkrn gviiagycve
      361 gtlakhimse peeittmsgq klplkmsvdy isfsahtdyq qtsefiralk pphvilvhge
      421 qnemarlkaa lireyednde vhievhnprn teavtlnfrg eklakvmgfl adkkpeqgqr
      481 vsgilvkrnf nyhilspcdl snytdlamst vkqtqaipyt gpfnllcyql qkltgdveel
      541 eiqekpalkv fknitviqep gmvvlewlan psndmyadtv ttvilevqsn pkirkgavqk
      601 vskklemhvy skrleimlqd ifgedcvsvk ddsilsvtvd gktanlnlet rtveceegse
      661 ddeslremve laaqrlyeal tpvh
//
