LOCUS       NM_001321833            2488 bp    mRNA    linear   PRI 24-JUN-2024
DEFINITION  Homo sapiens cleavage and polyadenylation specific factor 3
            (CPSF3), transcript variant 2, mRNA.
ACCESSION   NM_001321833 XM_005246168
VERSION     NM_001321833.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Zhang,Y., Liu,D., Guo,D., Lin,W., Lu,W., Hu,L., Chen,S. and Chen,C.
  TITLE     CPSF3 regulates alternative polyadenylation of CNIH2 to promote
            esophageal squamous cell carcinoma progression
  JOURNAL   Cancer Lett 593, 216925 (2024)
   PUBMED   38718887
  REMARK    GeneRIF: CPSF3 regulates alternative polyadenylation of CNIH2 to
            promote esophageal squamous cell carcinoma progression.
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Alahmari,A.A., Chaubey,A.H., Jonnakuti,V.S., Tisdale,A.A.,
            Schwarz,C.D., Cornwell,A.C., Maraszek,K.E., Paterson,E.J., Kim,M.,
            Venkat,S., Gomez,E.C., Wang,J., Gurova,K.V., Yalamanchili,H.K. and
            Feigin,M.E.
  TITLE     CPSF3 inhibition blocks pancreatic cancer cell proliferation
            through disruption of core histone mRNA processing
  JOURNAL   RNA 30 (3), 281-297 (2024)
   PUBMED   38191171
  REMARK    GeneRIF: CPSF3 inhibition blocks pancreatic cancer cell
            proliferation through disruption of core histone mRNA processing.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2488)
  AUTHORS   Shen,P., Ye,K., Xiang,H., Zhang,Z., He,Q., Zhang,X., Cai,M.C.,
            Chen,J., Sun,Y., Lin,L., Qi,C., Zhang,M., Cheung,L.W.T., Shi,T.,
            Yin,X., Li,Y., Di,W., Zang,R., Tan,L. and Zhuang,G.
  TITLE     Therapeutic targeting of CPSF3-dependent transcriptional
            termination in ovarian cancer
  JOURNAL   Sci Adv 9 (47), eadj0123 (2023)
   PUBMED   37992178
  REMARK    GeneRIF: Therapeutic targeting of CPSF3-dependent transcriptional
            termination in ovarian cancer.
REFERENCE   4  (bases 1 to 2488)
  AUTHORS   Huang,J., Liu,X., Sun,Y., Li,Z., Lin,M.H., Hamilton,K.,
            Mandel,C.R., Sandmeir,F., Conti,E., Oyala,P.H. and Tong,L.
  TITLE     An examination of the metal ion content in the active sites of
            human endonucleases CPSF73 and INTS11
  JOURNAL   J Biol Chem 299 (4), 103047 (2023)
   PUBMED   36822327
  REMARK    GeneRIF: An examination of the metal ion content in the active
            sites of human endonucleases CPSF73 and INTS11.
REFERENCE   5  (bases 1 to 2488)
  AUTHORS   Arnadottir,G.A., Oddsson,A., Jensson,B.O., Gisladottir,S.,
            Simon,M.T., Arnthorsson,A.O., Katrinardottir,H., Fridriksdottir,R.,
            Ivarsdottir,E.V., Jonasdottir,A., Jonasdottir,A., Barrick,R.,
            Saemundsdottir,J., le Roux,L., Oskarsson,G.R., Asmundsson,J.,
            Steffensen,T., Gudmundsson,K.R., Ludvigsson,P., Jonsson,J.J.,
            Masson,G., Jonsdottir,I., Holm,H., Jonasson,J.G., Magnusson,O.T.,
            Thorarensen,O., Abdenur,J., Norddahl,G.L., Gudbjartsson,D.F.,
            Bjornsson,H.T., Thorsteinsdottir,U., Sulem,P. and Stefansson,K.
  TITLE     Population-level deficit of homozygosity unveils CPSF3 as an
            intellectual disability syndrome gene
  JOURNAL   Nat Commun 13 (1), 705 (2022)
   PUBMED   35121750
  REMARK    GeneRIF: Population-level deficit of homozygosity unveils CPSF3 as
            an intellectual disability syndrome gene.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2488)
  AUTHORS   McCracken,S., Fong,N., Yankulov,K., Ballantyne,S., Pan,G.,
            Greenblatt,J., Patterson,S.D., Wickens,M. and Bentley,D.L.
  TITLE     The C-terminal domain of RNA polymerase II couples mRNA processing
            to transcription
  JOURNAL   Nature 385 (6614), 357-361 (1997)
   PUBMED   9002523
REFERENCE   7  (bases 1 to 2488)
  AUTHORS   Jenny,A., Minvielle-Sebastia,L., Preker,P.J. and Keller,W.
  TITLE     Sequence similarity between the 73-kilodalton protein of mammalian
            CPSF and a subunit of yeast polyadenylation factor I
  JOURNAL   Science 274 (5292), 1514-1517 (1996)
   PUBMED   8929409
REFERENCE   8  (bases 1 to 2488)
  AUTHORS   Murthy,K.G. and Manley,J.L.
  TITLE     The 160-kD subunit of human cleavage-polyadenylation specificity
            factor coordinates pre-mRNA 3'-end formation
  JOURNAL   Genes Dev 9 (21), 2672-2683 (1995)
   PUBMED   7590244
REFERENCE   9  (bases 1 to 2488)
  AUTHORS   Jenny,A., Hauri,H.P. and Keller,W.
  TITLE     Characterization of cleavage and polyadenylation specificity factor
            and cloning of its 100-kilodalton subunit
  JOURNAL   Mol Cell Biol 14 (12), 8183-8190 (1994)
   PUBMED   7969155
REFERENCE   10 (bases 1 to 2488)
  AUTHORS   Thuresson,A.C., Astrom,J., Astrom,A., Gronvik,K.O. and Virtanen,A.
  TITLE     Multiple forms of poly(A) polymerases in human cells
  JOURNAL   Proc Natl Acad Sci U S A 91 (3), 979-983 (1994)
   PUBMED   8302877
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC420903.1, DA455023.1,
            BC014106.1 and AA635764.1.
            
            On Aug 13, 2020 this sequence version replaced NM_001321833.1.
            
            Summary: This gene encodes a member of the metallo-beta-lactamase
            family. The encoded protein is a 73kDa subunit of the cleavage and
            polyadenylation specificity factor and functions as an endonuclease
            that recognizes the pre-mRNA 3'-cleavage site AAUAAA prior to
            polyadenylation. It also cleaves after the pre-mRNA sequence ACCCA
            during histone 3'-end pre-mRNA processing. [provided by RefSeq, Oct
            2012].
            
            Transcript Variant: This variant (2) and variant 3 both encode the
            same isoform (b).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038197.248371.1,
                                           SRR14038194.1727071.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-170               DC420903.1         118-287
            171-561             DA455023.1         174-564
            562-2479            BC014106.1         1240-3157
            2480-2488           AA635764.1         26-34               c
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p25.1"
     gene            1..2488
                     /gene="CPSF3"
                     /gene_synonym="CPSF-73; CPSF73; NEDMHS; NEDMHSN"
                     /note="cleavage and polyadenylation specific factor 3"
                     /db_xref="GeneID:51692"
                     /db_xref="HGNC:HGNC:2326"
                     /db_xref="MIM:606029"
     exon            1..399
                     /gene="CPSF3"
                     /gene_synonym="CPSF-73; CPSF73; NEDMHS; NEDMHSN"
                     /inference="alignment:Splign:2.1.0"
     exon            400..463
                     /gene="CPSF3"
                     /gene_synonym="CPSF-73; CPSF73; NEDMHS; NEDMHSN"
                     /inference="alignment:Splign:2.1.0"
     CDS             461..2404
                     /gene="CPSF3"
                     /gene_synonym="CPSF-73; CPSF73; NEDMHS; NEDMHSN"
                     /note="isoform b is encoded by transcript variant 2;
                     cleavage and polyadenylation specificity factor subunit 3;
                     mRNA 3'-end-processing endonuclease CPSF-73; cleavage and
                     polyadenylation specific factor 3, 73kDa"
                     /codon_start=1
                     /product="cleavage and polyadenylation specificity factor
                     subunit 3 isoform b"
                     /protein_id="NP_001308762.1"
                     /db_xref="CCDS:CCDS82417.1"
                     /db_xref="GeneID:51692"
                     /db_xref="HGNC:HGNC:2326"
                     /db_xref="MIM:606029"
                     /translation="MLDCGIHPGLEGMDALPYIDLIDPAEIDLLLISHFHLDHCGALP
                     WFLQKTSFKGRTFMTHATKAIYRWLLSDYVKVSNISADDMLYTETDLEESMDKIETIN
                     FHEVKEVAGIKFWCYHAGHVLGAAMFMIEIAGVKLLYTGDFSRQEDRHLMAAEIPNIK
                     PDILIIESTYGTHIHEKREEREARFCNTVHDIVNRGGRGLIPVFALGRAQELLLILDE
                     YWQNHPELHDIPIYYASSLAKKCMAVYQTYVNAMNDKIRKQININNPFVFKHISNLKS
                     MDHFDDIGPSVVMASPGMMQSGLSRELFESWCTDKRNGVIIAGYCVEGTLAKHIMSEP
                     EEITTMSGQKLPLKMSVDYISFSAHTDYQQTSEFIRALKPPHVILVHGEQNEMARLKA
                     ALIREYEDNDEVHIEVHNPRNTEAVTLNFRGEKLAKVMGFLADKKPEQGQRVSGILVK
                     RNFNYHILSPCDLSNYTDLAMSTVKQTQAIPYTGPFNLLCYQLQKLTGDVEELEIQEK
                     PALKVFKNITVIQEPGMVVLEWLANPSNDMYADTVTTVILEVQSNPKIRKGAVQKVSK
                     KLEMHVYSKRLEIMLQDIFGEDCVSVKDDSILSVTVDGKTANLNLETRTVECEEGSED
                     DESLREMVELAAQRLYEALTPVH"
     exon            464..561
                     /gene="CPSF3"
                     /gene_synonym="CPSF-73; CPSF73; NEDMHS; NEDMHSN"
                     /inference="alignment:Splign:2.1.0"
     exon            562..690
                     /gene="CPSF3"
                     /gene_synonym="CPSF-73; CPSF73; NEDMHS; NEDMHSN"
                     /inference="alignment:Splign:2.1.0"
     exon            691..868
                     /gene="CPSF3"
                     /gene_synonym="CPSF-73; CPSF73; NEDMHS; NEDMHSN"
                     /inference="alignment:Splign:2.1.0"
     exon            869..958
                     /gene="CPSF3"
                     /gene_synonym="CPSF-73; CPSF73; NEDMHS; NEDMHSN"
                     /inference="alignment:Splign:2.1.0"
     exon            959..1109
                     /gene="CPSF3"
                     /gene_synonym="CPSF-73; CPSF73; NEDMHS; NEDMHSN"
                     /inference="alignment:Splign:2.1.0"
     exon            1110..1285
                     /gene="CPSF3"
                     /gene_synonym="CPSF-73; CPSF73; NEDMHS; NEDMHSN"
                     /inference="alignment:Splign:2.1.0"
     exon            1286..1444
                     /gene="CPSF3"
                     /gene_synonym="CPSF-73; CPSF73; NEDMHS; NEDMHSN"
                     /inference="alignment:Splign:2.1.0"
     exon            1445..1591
                     /gene="CPSF3"
                     /gene_synonym="CPSF-73; CPSF73; NEDMHS; NEDMHSN"
                     /inference="alignment:Splign:2.1.0"
     exon            1592..1744
                     /gene="CPSF3"
                     /gene_synonym="CPSF-73; CPSF73; NEDMHS; NEDMHSN"
                     /inference="alignment:Splign:2.1.0"
     exon            1745..1853
                     /gene="CPSF3"
                     /gene_synonym="CPSF-73; CPSF73; NEDMHS; NEDMHSN"
                     /inference="alignment:Splign:2.1.0"
     exon            1854..1952
                     /gene="CPSF3"
                     /gene_synonym="CPSF-73; CPSF73; NEDMHS; NEDMHSN"
                     /inference="alignment:Splign:2.1.0"
     exon            1953..2047
                     /gene="CPSF3"
                     /gene_synonym="CPSF-73; CPSF73; NEDMHS; NEDMHSN"
                     /inference="alignment:Splign:2.1.0"
     exon            2048..2135
                     /gene="CPSF3"
                     /gene_synonym="CPSF-73; CPSF73; NEDMHS; NEDMHSN"
                     /inference="alignment:Splign:2.1.0"
     exon            2136..2205
                     /gene="CPSF3"
                     /gene_synonym="CPSF-73; CPSF73; NEDMHS; NEDMHSN"
                     /inference="alignment:Splign:2.1.0"
     exon            2206..2302
                     /gene="CPSF3"
                     /gene_synonym="CPSF-73; CPSF73; NEDMHS; NEDMHSN"
                     /inference="alignment:Splign:2.1.0"
     exon            2303..2488
                     /gene="CPSF3"
                     /gene_synonym="CPSF-73; CPSF73; NEDMHS; NEDMHSN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2461..2466
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CPSF3"
                     /gene_synonym="CPSF-73; CPSF73; NEDMHS; NEDMHSN"
                     /note="hexamer: AATAAA"
     polyA_site      2488
                     /gene="CPSF3"
                     /gene_synonym="CPSF-73; CPSF73; NEDMHS; NEDMHSN"
                     /note="major polyA site"
ORIGIN      
        1 ggaagttgtg ctcttggtga atggggttct tcctttttta tttaccggtg gctgtgcttc
       61 caatttagga agaccccggc gacctgttcc tcacccccgc ttcgccctca cactttcggg
      121 atgtctgcga ttcctgctga ggagagcgac cagctgctga tccgacccct gtaagggacc
      181 agcgagagag ggaatgaagc cacgggctgt gaggggcgcg aggggtaacc ggagactggc
      241 ctcctccgtc gcccgcgctc ccacctaccc cggcagcctg cctttggtcc gcgcttgcgg
      301 gccaggcttc tcaggctcga ggggttggag gaccgttgat cggaaggggc ctgctataga
      361 gtgaacggga ttttgctttt tcatcagttt caggggaggt ggagctgggc aagaagtagg
      421 aagatcatgt attattctcg agttcaaagg aagaaaaata atgctcgact gtgggatcca
      481 ccctggccta gaaggaatgg atgctcttcc ttatattgat ttaattgacc cagctgagat
      541 tgatctccta ttaattagtc atttccattt ggatcactgt ggagctctgc cctggtttct
      601 acagaagaca agtttcaaag gaagaacatt tatgactcat gccacaaaag ctatttatag
      661 atggcttctt tctgattatg tcaaagttag taacatatca gcagacgaca tgctgtatac
      721 cgagacagat ttggaagaaa gcatggacaa aattgaaact atcaactttc atgaagttaa
      781 ggaagttgcg ggaatcaagt tttggtgtta ccatgcaggt cacgtcctag gagccgccat
      841 gttcatgatt gagatcgcag gcgtgaagct tttgtacact ggtgatttct caagacaaga
      901 agataggcac ttaatggcag ctgaaattcc taatattaag cctgatattc ttatcattga
      961 atctacttat gggacccata tccatgagaa acgtgaagag cgagaagcaa gattctgtaa
     1021 cactgtccac gatattgtaa acagaggagg caggggtctc attcctgtct ttgctcttgg
     1081 aagggctcag gagctgctct tgattctaga tgagtactgg cagaatcacc cagaactaca
     1141 tgacattcca atatactatg catcatcttt ggccaagaag tgtatggcag tgtaccagac
     1201 atatgtaaat gccatgaatg acaaaatccg caaacagatc aacatcaata atccctttgt
     1261 tttcaaacac attagtaacc tcaagagcat ggatcatttt gatgacattg gtcccagtgt
     1321 tgtaatggcc tccccaggca tgatgcaaag tggcttatcc agagaattat ttgaaagctg
     1381 gtgtactgat aagaggaatg gtgtcattat agcgggatac tgtgtagaag ggacacttgc
     1441 caagcacatc atgtctgaac ctgaagaaat cactactatg tctggacaga agttaccact
     1501 gaaaatgtct gttgattaca tttctttctc agctcacacg gattaccagc aaaccagtga
     1561 atttattcgt gctttgaaac cgcctcatgt gattttagtc catggagaac agaatgaaat
     1621 ggccagattg aaagcagcac tgattcgaga atatgaagat aacgatgaag ttcacataga
     1681 ggttcataat cctcggaata cagaagcagt gaccttaaac ttcagaggag aaaaactagc
     1741 caaggttatg ggatttttag cagacaaaaa accagaacaa ggccagcggg tctcaggaat
     1801 acttgttaaa agaaacttta attatcacat actttctcct tgcgacctgt ccaattatac
     1861 tgacctggcc atgagcacgg tgaagcagac ccaagccatt ccatatactg gtccctttaa
     1921 tttgctctgt taccagctgc agaaattgac aggtgatgtg gaagaattag aaattcaaga
     1981 aaaacctgct ctgaaagtgt tcaaaaatat tactgtaata caagaaccag gcatggtggt
     2041 attagaatgg ctggcaaacc cttctaatga tatgtatgca gatacagtaa caactgtgat
     2101 attggaagtt cagtcaaatc ccaaaataag aaaaggtgca gtacagaagg tttctaaaaa
     2161 attagaaatg cacgtttaca gcaagaggtt ggagatcatg ctccaggaca tatttggaga
     2221 agactgtgta agtgtaaagg atgactctat tcttagcgtc acagtggacg ggaaaactgc
     2281 caaccttaac ttggagacac ggactgtaga atgtgaagag ggaagtgaag acgatgaatc
     2341 cctccgagaa atggtggagc tggctgcaca gagactgtac gaggccctga cgccagttca
     2401 ctgagactgt gcctgtatat gaactttgaa aaaatacttg actctacttt tgttacctaa
     2461 aataaaatgc attcgtttct ctggggga
//
