LOCUS       NM_013291               4480 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens cleavage and polyadenylation specific factor 1
            (CPSF1), mRNA.
ACCESSION   NM_013291
VERSION     NM_013291.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Chen,X., Lloyd,S.M., Kweon,J., Gamalong,G.M. and Bao,X.
  TITLE     Epidermal progenitors suppress GRHL3-mediated differentiation
            through intronic polyadenylation promoted by CPSF-HNRNPA3
            collaboration
  JOURNAL   Nat Commun 12 (1), 448 (2021)
   PUBMED   33469008
  REMARK    GeneRIF: Epidermal progenitors suppress GRHL3-mediated
            differentiation through intronic polyadenylation promoted by
            CPSF-HNRNPA3 collaboration.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 4480)
  AUTHORS   Ouyang,J., Sun,W., Xiao,X., Li,S., Jia,X., Zhou,L., Wang,P. and
            Zhang,Q.
  TITLE     CPSF1 mutations are associated with early-onset high myopia and
            involved in retinal ganglion cell axon projection
  JOURNAL   Hum Mol Genet 28 (12), 1959-1970 (2019)
   PUBMED   30689892
  REMARK    GeneRIF: Heterozygous loss-of-function mutations in CPSF1 are
            associated with early-onset high myopia and that CPSF1 may play an
            important role in the development of retinal ganglion cell axon
            projection.
REFERENCE   4  (bases 1 to 4480)
  AUTHORS   Clerici,M., Faini,M., Muckenfuss,L.M., Aebersold,R. and Jinek,M.
  TITLE     Structural basis of AAUAAA polyadenylation signal recognition by
            the human CPSF complex
  JOURNAL   Nat Struct Mol Biol 25 (2), 135-138 (2018)
   PUBMED   29358758
  REMARK    GeneRIF: cryo-electron microscopic analysis of a core CPSF module
            bound to the polyadenylation signal hexamer motif
            Erratum:[Nat Struct Mol Biol. 2018 Apr;25(4):355. doi:
            10.1038/s41594-018-0044-6.. PMID: 29540792]
REFERENCE   5  (bases 1 to 4480)
  AUTHORS   Zhang,B., Liu,Y., Liu,D. and Yang,L.
  TITLE     Targeting cleavage and polyadenylation specific factor 1 via shRNA
            inhibits cell proliferation in human ovarian cancer
  JOURNAL   J Biosci 42 (3), 417-425 (2017)
   PUBMED   29358555
  REMARK    GeneRIF: Knockdown of CPSF inhibits the proliferation, migration
            and invasion of ovarian carcinoma cells.
REFERENCE   6  (bases 1 to 4480)
  AUTHORS   Schul,W., Groenhout,B., Koberna,K., Takagaki,Y., Jenny,A.,
            Manders,E.M., Raska,I., van Driel,R. and de Jong,L.
  TITLE     The RNA 3' cleavage factors CstF 64 kDa and CPSF 100 kDa are
            concentrated in nuclear domains closely associated with coiled
            bodies and newly synthesized RNA
  JOURNAL   EMBO J 15 (11), 2883-2892 (1996)
   PUBMED   8654386
REFERENCE   7  (bases 1 to 4480)
  AUTHORS   Murthy,K.G. and Manley,J.L.
  TITLE     The 160-kD subunit of human cleavage-polyadenylation specificity
            factor coordinates pre-mRNA 3'-end formation
  JOURNAL   Genes Dev 9 (21), 2672-2683 (1995)
   PUBMED   7590244
REFERENCE   8  (bases 1 to 4480)
  AUTHORS   Jenny,A. and Keller,W.
  TITLE     Cloning of cDNAs encoding the 160 kDa subunit of the bovine
            cleavage and polyadenylation specificity factor
  JOURNAL   Nucleic Acids Res 23 (14), 2629-2635 (1995)
   PUBMED   7651824
REFERENCE   9  (bases 1 to 4480)
  AUTHORS   Thuresson,A.C., Astrom,J., Astrom,A., Gronvik,K.O. and Virtanen,A.
  TITLE     Multiple forms of poly(A) polymerases in human cells
  JOURNAL   Proc Natl Acad Sci U S A 91 (3), 979-983 (1994)
   PUBMED   8302877
REFERENCE   10 (bases 1 to 4480)
  AUTHORS   Keller,W., Bienroth,S., Lang,K.M. and Christofori,G.
  TITLE     Cleavage and polyadenylation factor CPF specifically interacts with
            the pre-mRNA 3' processing signal AAUAAA
  JOURNAL   EMBO J 10 (13), 4241-4249 (1991)
   PUBMED   1756731
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CD299980.1 and BC017232.2.
            
            On Nov 22, 2018 this sequence version replaced NM_013291.2.
            
            Summary: Cleavage and polyadenylation specificity factor (CPSF) is
            a multisubunit complex that plays a central role in 3-prime
            processing of pre-mRNAs. CPSF recognizes the AAUAAA signal in the
            pre-mRNA and interacts with other proteins to facilitate both RNA
            cleavage and poly(A) synthesis. CPSF1 is the largest subunit of the
            CPSF complex (Murthy and Manley, 1995 [PubMed 7590244]).[supplied
            by OMIM, Mar 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC017232.2, U37012.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000616140.2/ ENSP00000484669.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                CD299980.1         15-30
            17-4480             BC017232.2         1-4464
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q24.3"
     gene            1..4480
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /note="cleavage and polyadenylation specific factor 1"
                     /db_xref="GeneID:29894"
                     /db_xref="HGNC:HGNC:2324"
                     /db_xref="MIM:606027"
     exon            1..47
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            48..205
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     CDS             62..4393
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /EC_number="2.4.2.30"
                     /note="polyadenylation specificity factor; CPSF 160 kDa
                     subunit; cleavage and polyadenylation specificity factor
                     160 kDa subunit; cleavage and polyadenylation specific
                     factor 1, 160kDa"
                     /codon_start=1
                     /product="cleavage and polyadenylation specificity factor
                     subunit 1"
                     /protein_id="NP_037423.2"
                     /db_xref="CCDS:CCDS34966.1"
                     /db_xref="GeneID:29894"
                     /db_xref="HGNC:HGNC:2324"
                     /db_xref="MIM:606027"
                     /translation="MYAVYKQAHPPTGLEFSMYCNFFNNSERNLVVAGTSQLYVYRLN
                     RDAEALTKNDRSTEGKAHREKLELAASFSFFGNVMSMASVQLAGAKRDALLLSFKDAK
                     LSVVEYDPGTHDLKTLSLHYFEEPELRDGFVQNVHTPRVRVDPDGRCAAMLVYGTRLV
                     VLPFRRESLAEEHEGLVGEGQRSSFLPSYIIDVRALDEKLLNIIDLQFLHGYYEPTLL
                     ILFEPNQTWPGRVAVRQDTCSIVAISLNITQKVHPVIWSLTSLPFDCTQALAVPKPIG
                     GVVVFAVNSLLYLNQSVPPYGVALNSLTTGTTAFPLRTQEGVRITLDCAQATFISYDK
                     MVISLKGGEIYVLTLITDGMRSVRAFHFDKAAASVLTTSMVTMEPGYLFLGSRLGNSL
                     LLKYTEKLQEPPASAVREAADKEEPPSKKKRVDATAGWSAAGKSVPQDEVDEIEVYGS
                     EAQSGTQLATYSFEVCDSILNIGPCANAAVGEPAFLSEEFQNSPEPDLEIVVCSGHGK
                     NGALSVLQKSIRPQVVTTFELPGCYDMWTVIAPVRKEEEDNPKGEGTEQEPSTTPEAD
                     DDGRRHGFLILSREDSTMILQTGQEIMELDTSGFATQGPTVFAGNIGDNRYIVQVSPL
                     GIRLLEGVNQLHFIPVDLGAPIVQCAVADPYVVIMSAEGHVTMFLLKSDSYGGRHHRL
                     ALHKPPLHHQSKVITLCLYRDLSGMFTTESRLGGARDELGGRSGPEAEGLGSETSPTV
                     DDEEEMLYGDSGSLFSPSKEEARRSSQPPADRDPAPFRAEPTHWCLLVRENGTMEIYQ
                     LPDWRLVFLVKNFPVGQRVLVDSSFGQPTTQGEARREEATRQGELPLVKEVLLVALGS
                     RQSRPYLLVHVDQELLIYEAFPHDSQLGQGNLKVRFKKVPHNINFREKKPKPSKKKAE
                     GGGAEEGAGARGRVARFRYFEDIYGYSGVFICGPSPHWLLVTGRGALRLHPMAIDGPV
                     DSFAPFHNVNCPRGFLYFNRQGELRISVLPAYLSYDAPWPVRKIPLRCTAHYVAYHVE
                     SKVYAVATSTNTPCARIPRMTGEEKEFETIERDERYIHPQQEAFSIQLISPVSWEAIP
                     NARIELQEWEHVTCMKTVSLRSEETVSGLKGYVAAGTCLMQGEEVTCRGRILIMDVIE
                     VVPEPGQPLTKNKFKVLYEKEQKGPVTALCHCNGHLVSAIGQKIFLWSLRASELTGMA
                     FIDTQLYIHQMISVKNFILAADVMKSISLLRYQEESKTLSLVSRDAKPLEVYSVDFMV
                     DNAQLGFLVSDRDRNLMVYMYLPEAKESFGGMRLLRRADFHVGAHVNTFWRTPCRGAT
                     EGLSKKSVVWENKHITWFATLDGGIGLLLPMQEKTYRRLLMLQNALTTMLPHHAGLNP
                     RAFRMLHVDRRTLQNAVRNVLDGELLNRYLYLSTMERSELAKKIGTTPDIILDDLLET
                     DRVTAHF"
     exon            206..233
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            234..367
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            368..448
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            449..600
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            601..747
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            748..887
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            888..998
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            999..1092
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            1093..1180
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            1181..1303
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            1304..1355
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            1356..1453
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            1454..1528
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            1529..1609
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            1610..1699
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            1700..1813
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            1814..1955
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            1956..2134
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            2135..2271
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            2272..2446
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            2447..2653
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            2654..2743
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            2744..2887
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            2888..3040
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            3041..3157
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            3158..3248
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            3249..3333
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            3334..3475
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            3476..3628
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            3629..3805
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            3806..3872
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            3873..3920
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            3921..4076
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            4077..4206
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            4207..4345
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     exon            4346..4480
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      4480
                     /gene="CPSF1"
                     /gene_synonym="CPSF160; HSU37012; MYP27; P/cl.18"
                     /note="major polyA site"
ORIGIN      
        1 gagttcgctg ctgtcccggt tcctctcgag tcggctccaa ctgccagccc gggttggcgc
       61 catgtacgcc gtgtacaaac aggcgcatcc gcccaccggt ctggagttct ccatgtactg
      121 caacttcttc aacaacagcg agcgcaacct ggtagtggcc gggacctcgc agctctacgt
      181 gtaccgcctc aaccgcgacg ccgaggctct gaccaagaat gacaggagca cagaggggaa
      241 ggcccaccgg gagaagctcg agcttgctgc ctccttctcc ttctttggca acgtcatgtc
      301 catggccagc gtgcagctgg caggagccaa gcgggatgcc ctgctcctaa gcttcaagga
      361 tgccaagctg tctgtggtgg agtacgaccc gggcacccat gacctgaaga ccctgtcact
      421 gcactacttt gaggagcctg agcttcggga cgggtttgtg cagaatgtac acacgccgcg
      481 agtgcgggtg gaccccgacg ggcgctgtgc agccatgctt gtctacggca cgcggctggt
      541 ggtcctgccc ttccgcaggg agagcctggc tgaggagcac gaggggctcg tgggtgaggg
      601 gcagaggtcc agcttcctgc ccagctacat catcgacgtg cgggccctag acgagaagct
      661 gctcaacatc atcgacctgc agttcctgca tggctactac gagcctaccc tcctcatcct
      721 gtttgagccc aaccagacct ggcctgggcg cgtggccgtg cggcaggaca cgtgctccat
      781 tgtggccatc tcactgaaca tcacgcagaa ggtgcacccc gtcatctggt ccctcaccag
      841 cctgcccttt gactgcaccc aggctctggc tgtgcccaag cccataggtg gggtggtggt
      901 gtttgccgtc aactcgctgt tgtacctgaa ccagagcgtc cccccgtatg gcgtggctct
      961 caacagcctc accacaggaa ccacggcttt cccgcttcgc acccaggagg gtgtgcggat
     1021 caccctggac tgcgcccagg ccaccttcat ctcctacgac aagatggtca tctccctcaa
     1081 gggcggcgag atctacgtgc tgaccctcat caccgacggc atgcgcagtg tccgagcgtt
     1141 ccactttgac aaggcggccg ccagcgtcct caccaccagc atggtcacca tggagcccgg
     1201 gtacctgttc ctgggttctc gcctgggcaa ttccctcctc ctcaagtaca cggagaagct
     1261 gcaggagccc ccggccagtg ctgtccgtga ggctgccgac aaggaagagc ctccctcaaa
     1321 gaagaagcga gtggatgcga cggccggctg gtcagctgcg ggtaagtcgg tgccgcagga
     1381 tgaggtggac gagattgaag tgtacggcag cgaggcccag tcgggaacac agctggccac
     1441 ctactccttt gaggtgtgtg acagcatcct gaacattgga ccctgtgcca atgccgccgt
     1501 gggcgagcct gccttcctct ctgaagagtt tcagaacagc cccgagccgg acctggagat
     1561 tgtggtttgc tccggccacg ggaagaacgg ggctttgtcg gtgctgcaga agagcatccg
     1621 gccccaggtg gtgacaacct ttgagcttcc cggctgctat gacatgtgga cagtcatcgc
     1681 cccggtgcgt aaggaggagg aggacaatcc caagggggag ggcacagagc aggaacccag
     1741 caccacccct gaagcagacg acgacggccg cagacacgga ttcctgattc tgagccggga
     1801 agactccacc atgatcctgc agacggggca ggagatcatg gagctggaca ccagtggctt
     1861 cgccactcag ggccccacgg tctttgctgg gaacatcggg gacaaccgct acattgtcca
     1921 agtgtcacca ctgggcatcc gcctgctgga aggagtgaat cagctgcact tcatccccgt
     1981 ggacctgggc gcccccatcg tgcagtgcgc cgtggccgac ccctatgtgg tcatcatgag
     2041 tgccgagggc cacgtcacca tgttcctgct gaagagtgac tcctacggtg gccgccacca
     2101 ccgcctggcg ctgcacaagc ccccgctgca ccatcagtcc aaggtgatta cgctgtgcct
     2161 gtaccgagac ctcagcggca tgttcaccac tgagagccgc ctgggtgggg cccgtgacga
     2221 gctcgggggc cgcagtggcc cggaggccga gggcctgggc tcagagacta gccccacagt
     2281 ggatgacgag gaggagatgc tgtatgggga ttcgggctcc ctcttcagcc ccagcaagga
     2341 ggaggcccga agaagcagcc agccccctgc tgaccgggac cctgcaccct tccgggcaga
     2401 gcctacccac tggtgcctgc tggtgcggga gaatggcacc atggagatct accagcttcc
     2461 cgactggcgg ctggtgttcc tggtgaagaa cttccctgtg gggcagcggg tccttgtgga
     2521 cagctccttt ggacagccca ctacacaggg cgaggcccgc agggaggagg ccacgcgcca
     2581 gggggagctg cccctcgtca aggaggtgct gctggtggcg ctgggcagcc gccagagcag
     2641 gccctacctg ctggtgcatg tggaccaaga gctgcttatc tacgaggcct tcccccacga
     2701 ctctcagctc ggccagggca atctcaaagt ccgctttaag aaggtccctc acaacatcaa
     2761 cttccgtgag aagaagccaa agccatccaa gaagaaagca gaaggtggcg gcgcagagga
     2821 gggggctggg gcccggggcc gcgtggcgcg tttccgctac ttcgaggata tttatggcta
     2881 ctcaggggtc ttcatctgcg gcccctcccc tcactggctc ttggtgaccg gccgaggggc
     2941 tctgcggcta caccccatgg ccatcgacgg cccggtcgac tctttcgctc cattccacaa
     3001 tgtcaactgt ccccgcggct tcctgtactt caacagacag ggcgagctga ggatcagtgt
     3061 cctgcctgcc tacctgtcct atgatgcccc atggcctgtc aggaagatcc cgctgcgctg
     3121 cacggcccac tatgtggctt accacgtgga gtctaaggtg tatgctgtgg ccaccagcac
     3181 caacacgccg tgtgcccgca tcccacgcat gactggcgag gagaaggagt ttgagaccat
     3241 cgagagagat gagcggtaca tccaccccca gcaggaggcc ttctccatcc agctcatctc
     3301 cccggtcagc tgggaggcta ttcccaatgc caggatcgag ctgcaggagt gggagcatgt
     3361 gacctgcatg aagacagtgt ctctgcgcag tgaggagacc gtgtcgggcc tcaaaggcta
     3421 cgtggccgcc gggacctgcc tcatgcaggg ggaggaggtc acgtgccgag ggcggatctt
     3481 gatcatggat gtgattgagg tggtgcccga gcctggccag cccttgacca agaacaagtt
     3541 caaagtcctt tacgagaagg agcagaaggg gcccgtgacc gccctgtgcc actgcaatgg
     3601 ccacctggtg tcggccatcg gccagaagat tttcctgtgg agcctgcggg ccagcgagct
     3661 gacgggcatg gccttcatcg acacgcagct ctacatacac cagatgatca gcgtcaagaa
     3721 cttcatcctg gcagccgacg tcatgaagag catttcgctg ctgcgctacc aggaggaaag
     3781 caagacgctg agcctggtgt cgcgggatgc caagcccctg gaggtgtaca gcgtggactt
     3841 catggtggac aatgcccagc tgggttttct ggtgtctgac cgcgaccgca acctcatggt
     3901 gtacatgtac ctgcccgaag ccaaggagag tttcgggggc atgcgcctgc tgcgtcgggc
     3961 agacttccac gtgggtgccc acgtgaacac gttctggagg accccgtgcc ggggggccac
     4021 tgaagggctc agcaaaaagt cggtcgtgtg ggagaataag cacatcacgt ggtttgccac
     4081 cctggacggc ggcatcgggc tgctgctgcc catgcaggag aagacctacc ggcggctgct
     4141 gatgctgcag aacgcgctga ccaccatgct gccacaccac gccggcctca acccccgcgc
     4201 cttccggatg ctgcacgtgg accgccgcac cctccagaat gccgtgcgca acgtgctgga
     4261 tggggagctg ctcaaccgct acctgtacct gagcaccatg gagcgcagcg agctagccaa
     4321 gaagatcggc accacaccag acataatcct ggacgacttg ctggagacgg accgcgtcac
     4381 cgcccacttc tagccccgtg gatgccgtca ccaccagcac acggaactac ctcccacccc
     4441 ctttttgtac aaaacacaag gaaaaacatt ttttgcttga
//
