LOCUS       NM_173077               1245 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens carboxypeptidase O (CPO), mRNA.
ACCESSION   NM_173077
VERSION     NM_173077.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Garcia-Guerrero,M.C., Garcia-Pardo,J., Berenguer,E.,
            Fernandez-Alvarez,R., Barfi,G.B., Lyons,P.J., Aviles,F.X.,
            Huber,R., Lorenzo,J. and Reverter,D.
  TITLE     Crystal structure and mechanism of human carboxypeptidase O:
            Insights into its specific activity for acidic residues
  JOURNAL   Proc Natl Acad Sci U S A 115 (17), E3932-E3939 (2018)
   PUBMED   29636417
  REMARK    GeneRIF: hCPO can also trim C-t extensions of proteins, such as
            epidermal growth factor, suggesting a role in the maturation and
            degradation of growth factors and bioactive peptides.
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   Fox,C.S., Liu,Y., White,C.C., Feitosa,M., Smith,A.V.,
            Heard-Costa,N., Lohman,K., Johnson,A.D., Foster,M.C.,
            Greenawalt,D.M., Griffin,P., Ding,J., Newman,A.B., Tylavsky,F.,
            Miljkovic,I., Kritchevsky,S.B., Launer,L., Garcia,M.,
            Eiriksdottir,G., Carr,J.J., Gudnason,V., Harris,T.B., Cupples,L.A.
            and Borecki,I.B.
  CONSRTM   GIANT Consortium; MAGIC Consortium; GLGC Consortium
  TITLE     Genome-wide association for abdominal subcutaneous and visceral
            adipose reveals a novel locus for visceral fat in women
  JOURNAL   PLoS Genet 8 (5), e1002695 (2012)
   PUBMED   22589738
REFERENCE   3  (bases 1 to 1245)
  AUTHORS   Lyons,P.J. and Fricker,L.D.
  TITLE     Carboxypeptidase O is a glycosylphosphatidylinositol-anchored
            intestinal peptidase with acidic amino acid specificity
  JOURNAL   J Biol Chem 286 (45), 39023-39032 (2011)
   PUBMED   21921028
  REMARK    GeneRIF: CPO cleaves acidic amino acids from dietary proteins and
            peptides, thus complementing the actions of well known digestive
            carboxypeptidases CPA and CPB.
REFERENCE   4  (bases 1 to 1245)
  AUTHORS   Wei,S., Segura,S., Vendrell,J., Aviles,F.X., Lanoue,E., Day,R.,
            Feng,Y. and Fricker,L.D.
  TITLE     Identification and characterization of three members of the human
            metallocarboxypeptidase gene family
  JOURNAL   J Biol Chem 277 (17), 14954-14964 (2002)
   PUBMED   11836249
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC019052.7, BC112078.1 and
            AJ422118.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_173077.2.
            
            Summary: This gene is a member of the metallocarboxypeptidase gene
            family. [provided by RefSeq, Jan 2011].
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC112078.1, AJ422118.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2153733, SAMEA2153932
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000272852.4/ ENSP00000272852.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-36                AC019052.7         58738-58773
            37-82               BC112078.1         1-46
            83-1207             AJ422118.1         1-1125
            1208-1245           BC112078.1         1172-1209
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q33.3"
     gene            1..1245
                     /gene="CPO"
                     /note="carboxypeptidase O"
                     /db_xref="GeneID:130749"
                     /db_xref="HGNC:HGNC:21011"
                     /db_xref="MIM:609563"
     exon            1..150
                     /gene="CPO"
                     /inference="alignment:Splign:2.1.0"
     CDS             83..1207
                     /gene="CPO"
                     /EC_number="3.4.17.-"
                     /note="metallocarboxypeptidase C; metallocarboxypeptidase
                     O"
                     /codon_start=1
                     /product="carboxypeptidase O precursor"
                     /protein_id="NP_775100.1"
                     /db_xref="CCDS:CCDS2372.1"
                     /db_xref="GeneID:130749"
                     /db_xref="HGNC:HGNC:21011"
                     /db_xref="MIM:609563"
                     /translation="MKPLLETLYLLGMLVPGGLGYDRSLAQHRQEIVDKSVSPWSLET
                     YSYNIYHPMGEIYEWMREISEKYKEVVTQHFLGVTYETHPMYYLKISQPSGNPKKIIW
                     MDCGIHAREWIAPAFCQWFVKEILQNHKDNSSIRKLLRNLDFYVLPVLNIDGYIYTWT
                     TDRLWRKSRSPHNNGTCFGTDLNRNFNASWCSIGASRNCQDQTFCGTGPVSEPETKAV
                     ASFIESKKDDILCFLTMHSYGQLILTPYGYTKNKSSNHPEMIQVGQKAANALKAKYGT
                     NYRVGSSADILYASSGSSRDWARDIGIPFSYTFELRDSGTYGFVLPEAQIQPTCEETM
                     EAVLSVLDDVYAKHWHSDSAGRVTSATMLLGLLVSCMSLL"
     exon            151..247
                     /gene="CPO"
                     /inference="alignment:Splign:2.1.0"
     exon            248..349
                     /gene="CPO"
                     /inference="alignment:Splign:2.1.0"
     exon            350..454
                     /gene="CPO"
                     /inference="alignment:Splign:2.1.0"
     exon            455..565
                     /gene="CPO"
                     /inference="alignment:Splign:2.1.0"
     exon            566..656
                     /gene="CPO"
                     /inference="alignment:Splign:2.1.0"
     exon            657..859
                     /gene="CPO"
                     /inference="alignment:Splign:2.1.0"
     exon            860..944
                     /gene="CPO"
                     /inference="alignment:Splign:2.1.0"
     exon            945..1245
                     /gene="CPO"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 acagtcttga tgcattcatt ctcaaggaca cttgatccac tgccagagag gcccagaatt
       61 ttctaactta ctgtgtggca gaatgaagcc tctgcttgaa accctttatc ttttggggat
      121 gctggttcct ggagggctgg gatatgatag atccttagcc caacacagac aagagattgt
      181 ggacaagtca gtgagtccat ggagcctgga gacgtattcc tataacatat accaccccat
      241 gggagagatc tatgagtgga tgagagagat cagtgagaag tacaaggaag tggtgacaca
      301 gcatttccta ggagtgacct atgagaccca ccccatgtat tatctgaaga tcagccaacc
      361 atctggtaat cccaagaaaa tcatttggat ggactgtgga attcacgcca gagaatggat
      421 tgctcctgct ttttgccaat ggttcgtcaa agaaattcta caaaaccata aagacaactc
      481 aagtatacgc aagctcctta ggaacctgga cttctatgtc cttccagttc ttaacataga
      541 tggttatatc tacacttgga caactgatcg tctttggagg aaatcccgtt caccccataa
      601 taatggcaca tgttttggga cggatctcaa tcgaaatttc aatgcatctt ggtgtagtat
      661 tggtgcctct agaaactgcc aagatcaaac attctgtggg acagggccag tgtctgaacc
      721 agagactaaa gctgttgcca gcttcataga gagcaagaag gatgatattt tgtgcttcct
      781 gaccatgcac tcttatgggc agttaattct cacaccttac ggctacacca aaaataaatc
      841 aagtaaccac ccagaaatga ttcaagttgg acagaaggca gcaaatgcat tgaaagcaaa
      901 gtatggaacc aattatagag ttggatcgag tgcagatatt ttatatgcct catcagggtc
      961 ttcaagagat tgggcccgag acattgggat tcccttctca tatacgtttg agctgaggga
     1021 cagtggaaca tatgggtttg ttctgccaga agctcagatc cagcccacct gtgaggagac
     1081 catggaggct gtgctgtcag tcctggatga tgtgtatgcg aaacactggc actcggacag
     1141 tgctggaagg gtgacatctg ccactatgct gctgggcctg ctggtgtcct gcatgtctct
     1201 tctctaagtg cattctgccc aggcctgctc aaccccagtg gcatg
//
