LOCUS       NM_006032               1995 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens copine 6 (CPNE6), transcript variant 2, mRNA.
ACCESSION   NM_006032
VERSION     NM_006032.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Liu,P., Khvotchev,M., Li,Y.C., Chanaday,N.L. and Kavalali,E.T.
  TITLE     Copine-6 Binds to SNAREs and Selectively Suppresses Spontaneous
            Neurotransmission
  JOURNAL   J Neurosci 38 (26), 5888-5899 (2018)
   PUBMED   29802203
  REMARK    GeneRIF: we identify copine-6 as a specific regulator of
            spontaneous neurotransmission
REFERENCE   3  (bases 1 to 1995)
  AUTHORS   Zhu,B., Zha,J., Long,Y., Hu,X., Chen,G. and Wang,X.
  TITLE     Increased expression of copine VI in patients with refractory
            epilepsy and a rat model
  JOURNAL   J Neurol Sci 360, 30-36 (2016)
   PUBMED   26723968
  REMARK    GeneRIF: expression of CPNE6 increased remarkably in brains of
            epileptic patients and in experimental epileptic rats. Expression
            is mainly in neurons.
REFERENCE   4  (bases 1 to 1995)
  AUTHORS   Yoo,J.C., Lim,Ty., Park,J.S., Hah,Y.S., Park,N., Hong,S.G.,
            Park,J.Y. and Yoon,T.J.
  TITLE     SYT14L, especially its C2 domain, is involved in regulating
            melanocyte differentiation
  JOURNAL   J Dermatol Sci 72 (3), 246-251 (2013)
   PUBMED   23999003
REFERENCE   5  (bases 1 to 1995)
  AUTHORS   Tomsig,J.L. and Creutz,C.E.
  TITLE     Copines: a ubiquitous family of Ca(2+)-dependent
            phospholipid-binding proteins
  JOURNAL   Cell Mol Life Sci 59 (9), 1467-1477 (2002)
   PUBMED   12440769
  REMARK    Review article
            Erratum:[Cell Mol Life Sci. 2003 Feb;60(2):430]
REFERENCE   6  (bases 1 to 1995)
  AUTHORS   Caudell,E.G., Caudell,J.J., Tang,C.H., Yu,T.K., Frederick,M.J. and
            Grimm,E.A.
  TITLE     Characterization of human copine III as a phosphoprotein with
            associated kinase activity
  JOURNAL   Biochemistry 39 (42), 13034-13043 (2000)
   PUBMED   11041869
REFERENCE   7  (bases 1 to 1995)
  AUTHORS   Nakayama,T., Yaoi,T., Kuwajima,G., Yoshie,O. and Sakata,T.
  TITLE     Ca2(+)-dependent interaction of N-copine, a member of the two C2
            domain protein family, with OS-9, the product of a gene frequently
            amplified in osteosarcoma
  JOURNAL   FEBS Lett 453 (1-2), 77-80 (1999)
   PUBMED   10403379
REFERENCE   8  (bases 1 to 1995)
  AUTHORS   Nakayama,T., Yaoi,T. and Kuwajima,G.
  TITLE     Localization and subcellular distribution of N-copine in mouse
            brain
  JOURNAL   J Neurochem 72 (1), 373-379 (1999)
   PUBMED   9886090
REFERENCE   9  (bases 1 to 1995)
  AUTHORS   Nakayama,T., Yaoi,T., Yasui,M. and Kuwajima,G.
  TITLE     N-copine: a novel two C2-domain-containing protein with neuronal
            activity-regulated expression
  JOURNAL   FEBS Lett 428 (1-2), 80-84 (1998)
   PUBMED   9645480
REFERENCE   10 (bases 1 to 1995)
  AUTHORS   Creutz,C.E., Tomsig,J.L., Snyder,S.L., Gautier,M.C., Skouri,F.,
            Beisson,J. and Cohen,J.
  TITLE     The copines, a novel class of C2 domain-containing,
            calcium-dependent, phospholipid-binding proteins conserved from
            Paramecium to humans
  JOURNAL   J Biol Chem 273 (3), 1393-1402 (1998)
   PUBMED   9430674
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB009288.1, AL136295.3 and
            BX118572.1.
            
            On Jun 2, 2019 this sequence version replaced NM_006032.3.
            
            Summary: This gene encodes a member of the copine family. Members
            of this family are calcium-dependent, phospholipid-binding proteins
            with C2 domains, two calcium- and phospholipid-binding domains.
            Through their domain structure and lipid binding capabilities,
            these proteins may play a role in membrane trafficking. This
            protein is thought to be brain-specific and has a domain structure
            of two N-terminal C2 domains and one von Willebrand factor A
            domain. It may have a role in synaptic plasticity. [provided by
            RefSeq, Jul 2013].
            
            Transcript Variant: This variant (2) differs in the 5' UTR and 5'
            coding region and initiates translation at a downstream start
            codon,, compared to variant 1. The encoded isoform (2) has a
            shorter N-terminus than isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.75432.1,
                                           SRR3476690.960319.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2144835, SAMEA2145743
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000689861.1/ ENSP00000510387.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1025              AB009288.1         16-1040
            1026-1026           AL136295.3         133866-133866       c
            1027-1406           AB009288.1         1042-1421
            1407-1407           AL136295.3         132855-132855       c
            1408-1910           AB009288.1         1423-1925
            1911-1995           BX118572.1         26-110              c
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q11.2"
     gene            1..1995
                     /gene="CPNE6"
                     /gene_synonym="copine-6"
                     /note="copine 6"
                     /db_xref="GeneID:9362"
                     /db_xref="HGNC:HGNC:2319"
                     /db_xref="MIM:605688"
     exon            1..80
                     /gene="CPNE6"
                     /gene_synonym="copine-6"
                     /inference="alignment:Splign:2.1.0"
     exon            81..252
                     /gene="CPNE6"
                     /gene_synonym="copine-6"
                     /inference="alignment:Splign:2.1.0"
     CDS             85..1758
                     /gene="CPNE6"
                     /gene_synonym="copine-6"
                     /note="isoform 2 is encoded by transcript variant 2;
                     N-copine; copine VI (neuronal)"
                     /codon_start=1
                     /product="copine-6 isoform 2"
                     /protein_id="NP_006023.1"
                     /db_xref="CCDS:CCDS9607.1"
                     /db_xref="GeneID:9362"
                     /db_xref="HGNC:HGNC:2319"
                     /db_xref="MIM:605688"
                     /translation="MSDPEMGWVPEPPTMTLGASRVELRVSCHGLLDRDTLTKPHPCV
                     LLKLYSDEQWVEVERTEVLRSCSSPVFSRVLALEYFFEEKQPLQFHVFDAEDGATSPR
                     NDTFLGSTECTLGQIVSQTKVTKPLLLKNGKTAGKSTITIVAEEVSGTNDYVQLTFRA
                     YKLDNKDLFSKSDPFMEIYKTNEDQSDQLVWRTEVVKNNLNPSWEPFRLSLHSLCSCD
                     VHRPLKFLVYDYDSSGKHDFIGEFTSTFQEMQEGTANPGQEMQWDCINPKYRDKKKNY
                     KSSGTVVLAQCTVEKVHTFLDYIMGGCQISFTVAIDFTASNGDPRSSQSLHCLSPRQP
                     NHYLQALRAVGGICQDYDSDKRFPAFGFGARIPPNFEVSHDFAINFDPENPECEEISG
                     VIASYRRCLPQIQLYGPTNVAPIINRVAEPAQREQSTGQATKYSVLLVLTDGVVSDMA
                     ETRTAIVRASRLPMSIIIVGVGNADFSDMRLLDGDDGPLRCPRGVPAARDIVQFVPFR
                     DFKDAAPSALAKCVLAEVPRQVVEYYASQGISPGAPRPCTLATTPSPSP"
     exon            253..432
                     /gene="CPNE6"
                     /gene_synonym="copine-6"
                     /inference="alignment:Splign:2.1.0"
     exon            433..507
                     /gene="CPNE6"
                     /gene_synonym="copine-6"
                     /inference="alignment:Splign:2.1.0"
     exon            508..582
                     /gene="CPNE6"
                     /gene_synonym="copine-6"
                     /inference="alignment:Splign:2.1.0"
     exon            583..666
                     /gene="CPNE6"
                     /gene_synonym="copine-6"
                     /inference="alignment:Splign:2.1.0"
     exon            667..756
                     /gene="CPNE6"
                     /gene_synonym="copine-6"
                     /inference="alignment:Splign:2.1.0"
     exon            757..861
                     /gene="CPNE6"
                     /gene_synonym="copine-6"
                     /inference="alignment:Splign:2.1.0"
     exon            862..948
                     /gene="CPNE6"
                     /gene_synonym="copine-6"
                     /inference="alignment:Splign:2.1.0"
     exon            949..1008
                     /gene="CPNE6"
                     /gene_synonym="copine-6"
                     /inference="alignment:Splign:2.1.0"
     exon            1009..1142
                     /gene="CPNE6"
                     /gene_synonym="copine-6"
                     /inference="alignment:Splign:2.1.0"
     exon            1143..1197
                     /gene="CPNE6"
                     /gene_synonym="copine-6"
                     /inference="alignment:Splign:2.1.0"
     exon            1198..1249
                     /gene="CPNE6"
                     /gene_synonym="copine-6"
                     /inference="alignment:Splign:2.1.0"
     exon            1250..1383
                     /gene="CPNE6"
                     /gene_synonym="copine-6"
                     /inference="alignment:Splign:2.1.0"
     exon            1384..1620
                     /gene="CPNE6"
                     /gene_synonym="copine-6"
                     /inference="alignment:Splign:2.1.0"
     exon            1621..1795
                     /gene="CPNE6"
                     /gene_synonym="copine-6"
                     /inference="alignment:Splign:2.1.0"
     exon            1796..1995
                     /gene="CPNE6"
                     /gene_synonym="copine-6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1973..1978
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CPNE6"
                     /gene_synonym="copine-6"
                     /note="hexamer: AATAAA"
     polyA_site      1995
                     /gene="CPNE6"
                     /gene_synonym="copine-6"
                     /note="major polyA site"
ORIGIN      
        1 ggcgccagcg gctggagcag caagggagtc agggccaggg ccagagagcc ggagagagga
       61 gcccccgacc gagagcccag tgacatgtcg gaccctgaga tgggatgggt gcctgagccc
      121 ccaaccatga cgctgggggc ctctcgggtg gagctgcggg tgtcctgcca tggcctcctg
      181 gaccgggaca cactcaccaa accccacccc tgcgtgctgc tcaagctcta ctctgatgag
      241 cagtgggtgg aggtagagcg cacagaggtg cttcgctcct gttccagccc tgtcttctcc
      301 cgggtgctgg cccttgagta tttttttgag gagaagcagc ccctgcagtt ccacgtgttc
      361 gatgccgagg acggagccac cagcccccga aatgatacct tcctcggctc tacggagtgc
      421 accttgggcc agattgtgtc acaaaccaag gtcactaagc cattattgct gaagaatggg
      481 aagactgcgg gcaagtccac catcacgatc gtggccgagg aggtatcagg cacaaacgac
      541 tatgtgcaac tcaccttcag agcctacaag ctggacaaca aggatctgtt cagcaagtct
      601 gaccctttca tggaaatcta taagaccaac gaggaccaaa gtgatcagct ggtctggaga
      661 actgaggtgg tgaagaacaa cctgaacccc agctgggagc cgttccgcct gtccctgcat
      721 tccctatgca gctgtgatgt tcaccgacct ctcaagttcc tggtgtatga ctatgactcc
      781 agtgggaagc atgacttcat cggcgagttc accagcactt tccaggagat gcaggaaggg
      841 acggcaaacc ctgggcagga gatgcagtgg gactgtatca accccaagta tcgggacaag
      901 aagaagaatt acaagagctc agggacggta gtgctggccc agtgcacggt ggagaaggtg
      961 cacaccttcc tggattacat catgggtggc tgccagatca gcttcacggt ggccattgac
     1021 ttcaccgcct ccaatgggga cccgaggagc agccagtccc tgcactgcct cagtccccga
     1081 cagcccaacc actacctgca ggccctgcgt gcagtgggag gcatctgcca ggactatgac
     1141 agtgataagc ggttcccagc ttttggcttt ggggctcgaa tcccccccaa cttcgaggtg
     1201 tcccatgact ttgctatcaa ctttgacccg gaaaatcctg aatgtgaaga gatctcaggg
     1261 gtcatcgcct cctaccgtcg ttgcctgccc cagatccagc tctacggccc caccaatgtg
     1321 gcccccatca tcaaccgtgt ggctgagccg gcccagcggg agcagagcac cggccaagcc
     1381 acgaagtact cggtgctgct ggtgctcact gacggtgtgg tgagcgacat ggctgagact
     1441 cgcactgcta tcgtgcgtgc ctcccgcctg cccatgtcca tcatcatcgt aggcgtgggc
     1501 aatgctgact tctctgacat gcggctgctg gatggcgacg acggcccctt gcgctgcccc
     1561 cgaggggtgc ctgcagcccg agacattgtc cagttcgtgc ccttccgaga cttcaaggat
     1621 gctgccccct ctgcactcgc caagtgtgtc ctggctgagg tgccacggca ggtggtggag
     1681 tactacgcca gccagggcat cagccctggg gctcccaggc cctgcacact ggctacgact
     1741 cccagcccta gcccgtgact gcctccctcc ggaccgacac tccctcagcc tctcagtgcc
     1801 tgtcctgacc ctcgtgactc cagtgaccaa tgcctccacc tcttggacca ggtgtgcccc
     1861 ctgggttctg gacgtgagtg gtgggtcctg ctcctatctc tccaaacccc atacccttca
     1921 atgctgtggc ccctcagtga cttccttggg tgatcctgac tttctagcca ttaataaaga
     1981 gaactgctcc tagca
//
