LOCUS       NM_001080513            3025 bp    mRNA    linear   PRI 03-APR-2024
DEFINITION  Homo sapiens carboxypeptidase N subunit 2 (CPN2), transcript
            variant 1, mRNA.
ACCESSION   NM_001080513 XM_925798
VERSION     NM_001080513.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Cui,R., Zhang,P. and Li,Y.
  TITLE     Role of Carboxypeptidase N Invasion and Migration in Breast Cancer
  JOURNAL   Anticancer Agents Med Chem 16 (9), 1198-1202 (2016)
   PUBMED   26860443
  REMARK    GeneRIF: Real-time PCR and western blotting show the following CPN2
            expression: minimal in MDA-MB-468 cells but obvious in MDA-MB-231
            and MCF7 cells. MDA-MB-231 breast cancer cell lines were selected
            for the control group and CPN2 was knocked out for the experimental
            group.
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Bastos-Amador,P., Royo,F., Gonzalez,E., Conde-Vancells,J.,
            Palomo-Diez,L., Borras,F.E. and Falcon-Perez,J.M.
  TITLE     Proteomic analysis of microvesicles from plasma of healthy donors
            reveals high individual variability
  JOURNAL   J Proteomics 75 (12), 3574-3584 (2012)
   PUBMED   22516433
REFERENCE   3  (bases 1 to 3025)
  AUTHORS   Grilo,A., Saez-Rosas,M.P., Santos-Morano,J., Sanchez,E.,
            Moreno-Rey,C., Real,L.M., Ramirez-Lorca,R. and Saez,M.E.
  TITLE     Identification of genetic factors associated with susceptibility to
            angiotensin-converting enzyme inhibitors-induced cough
  JOURNAL   Pharmacogenet Genomics 21 (1), 10-17 (2011)
   PUBMED   21052031
  REMARK    GeneRIF: Observational study of gene-disease association, gene-gene
            interaction, gene-environment interaction, and pharmacogenomic /
            toxicogenomic. (HuGE Navigator)
REFERENCE   4  (bases 1 to 3025)
  AUTHORS   Liu,T., Qian,W.J., Gritsenko,M.A., Camp,D.G. 2nd, Monroe,M.E.,
            Moore,R.J. and Smith,R.D.
  TITLE     Human plasma N-glycoproteome analysis by immunoaffinity
            subtraction, hydrazide chemistry, and mass spectrometry
  JOURNAL   J Proteome Res 4 (6), 2070-2080 (2005)
   PUBMED   16335952
REFERENCE   5  (bases 1 to 3025)
  AUTHORS   Bunkenborg,J., Pilch,B.J., Podtelejnikov,A.V. and Wisniewski,J.R.
  TITLE     Screening for N-glycosylated proteins by liquid chromatography mass
            spectrometry
  JOURNAL   Proteomics 4 (2), 454-465 (2004)
   PUBMED   14760718
REFERENCE   6  (bases 1 to 3025)
  AUTHORS   Riley,D.A., Tan,F., Miletich,D.J. and Skidgel,R.A.
  TITLE     Chromosomal localization of the genes for human carboxypeptidase D
            (CPD) and the active 50-kilodalton subunit of human
            carboxypeptidase N (CPN1)
  JOURNAL   Genomics 50 (1), 105-108 (1998)
   PUBMED   9628828
REFERENCE   7  (bases 1 to 3025)
  AUTHORS   Tan,F., Weerasinghe,D.K., Skidgel,R.A., Tamei,H., Kaul,R.K.,
            Roninson,I.B., Schilling,J.W. and Erdos,E.G.
  TITLE     The deduced protein sequence of the human carboxypeptidase N high
            molecular weight subunit reveals the presence of leucine-rich
            tandem repeats
  JOURNAL   J Biol Chem 265 (1), 13-19 (1990)
   PUBMED   2378615
  REMARK    Erratum:[J Biol Chem 1990 Jul 25;265(21):12749]
REFERENCE   8  (bases 1 to 3025)
  AUTHORS   Skidgel,R.A., Bennett,C.D., Schilling,J.W., Tan,F.L.,
            Weerasinghe,D.K. and Erdos,E.G.
  TITLE     Amino acid sequence of the N-terminus and selected tryptic peptides
            of the active subunit of human plasma carboxypeptidase N:
            comparison with other carboxypeptidases
  JOURNAL   Biochem Biophys Res Commun 154 (3), 1323-1329 (1988)
   PUBMED   3408501
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC174158.3.
            
            On Nov 22, 2018 this sequence version replaced NM_001080513.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Variants 1 and 2 encode the same protein.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC042334.1, BI762653.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1970526, SAMEA2159080
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000323830.4/ ENSP00000319464.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-87                AC174158.3         14473-14559
            88-3025             AC174158.3         23096-26033
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q29"
     gene            1..3025
                     /gene="CPN2"
                     /gene_synonym="ACBP"
                     /note="carboxypeptidase N subunit 2"
                     /db_xref="GeneID:1370"
                     /db_xref="HGNC:HGNC:2313"
                     /db_xref="MIM:603104"
     exon            1..87
                     /gene="CPN2"
                     /gene_synonym="ACBP"
                     /inference="alignment:Splign:2.1.0"
     exon            88..3025
                     /gene="CPN2"
                     /gene_synonym="ACBP"
                     /inference="alignment:Splign:2.1.0"
     CDS             91..1728
                     /gene="CPN2"
                     /gene_synonym="ACBP"
                     /EC_number="3.4.17.3"
                     /note="carboxypeptidase N, polypeptide 2, 83kD;
                     carboxypeptidase N large subunit; carboxypeptidase N 83
                     kDa chain; carboxypeptidase N regulatory subunit"
                     /codon_start=1
                     /product="carboxypeptidase N subunit 2 precursor"
                     /protein_id="NP_001073982.3"
                     /db_xref="CCDS:CCDS33920.1"
                     /db_xref="GeneID:1370"
                     /db_xref="HGNC:HGNC:2313"
                     /db_xref="MIM:603104"
                     /translation="MLPGAWLLWTSLLLLARPAQPCPMGCDCFVQEVFCSDEELATVP
                     LDIPPYTKNIIFVETSFTTLETRAFGSNPNLTKVVFLNTQLCQFRPDAFGGLPRLEDL
                     EVTGSSFLNLSTNIFSNLTSLGKLTLNFNMLEALPEGLFQHLAALESLHLQGNQLQAL
                     PRRLFQPLTHLKTLNLAQNLLAQLPEELFHPLTSLQTLKLSNNALSGLPQGVFGKLGS
                     LQELFLDSNNISELPPQVFSQLFCLERLWLQRNAITHLPLSIFASLGNLTFLSLQWNM
                     LRVLPAGLFAHTPCLVGLSLTHNQLETVAEGTFAHLSNLRSLMLSYNAITHLPAGIFR
                     DLEELVKLYLGSNNLTALHPALFQNLSKLELLSLSKNQLTTLPEGIFDTNYNLFNLAL
                     HGNPWQCDCHLAYLFNWLQQYTDRLLNIQTYCAGPAYLKGQVVPALNEKQLVCPVTRD
                     HLGFQVTWPDESKAGGSWDLAVQERAARSQCTYSNPEGTVVLACDQAQCRWLNVQLSP
                     QQGSLGLQYNASQEWDLRSSCGSLRLTVSIEARAAGP"
     polyA_site      2907
                     /gene="CPN2"
                     /gene_synonym="ACBP"
                     /note="major polyA site"
     regulatory      3006..3011
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CPN2"
                     /gene_synonym="ACBP"
                     /note="hexamer: AATAAA"
     polyA_site      3025
                     /gene="CPN2"
                     /gene_synonym="ACBP"
                     /note="major polyA site"
ORIGIN      
        1 ggattgagct gaccacaggc cacaccagac tcctctctgc tcctgaggaa gacagggcag
       61 cccggcgcca cccgctcggc cctcacgaag atgctccctg gagcctggct gctctggacc
      121 tccctcctgc tcctggccag gcctgcccag ccctgtccca tgggttgtga ctgcttcgtc
      181 caggaggtgt tctgctcaga tgaggagctt gccaccgtcc cactggacat cccgccatat
      241 acgaaaaaca tcatctttgt ggagacctcg ttcaccacat tggaaaccag agcttttggc
      301 agtaacccca acttgaccaa ggtggtcttc ctcaacactc agctctgcca gtttaggccg
      361 gatgccttcg gggggctgcc caggctggag gacctggagg tcacaggcag tagcttcttg
      421 aacctcagca ccaacatctt ctccaacctg acctcgctgg gcaagctcac cctcaacttc
      481 aacatgctgg aggctctgcc cgagggtctt ttccagcacc tggctgccct ggagtccctc
      541 cacctgcagg ggaaccagct ccaggccctg cccaggaggc tcttccagcc tctgacccat
      601 ctgaagacac tcaacctggc ccagaacctc ctggcccagc tcccggagga gctgttccac
      661 ccactcacca gcctgcagac cctgaagctg agcaacaacg cgctctctgg tctcccccag
      721 ggtgtgtttg gcaaactggg cagcctgcag gagctcttcc tggacagcaa caacatctcg
      781 gagctgcccc ctcaggtgtt ctcccagctc ttctgcctag agaggctgtg gctgcaacgc
      841 aacgccatca cgcacctgcc gctctccatc tttgcctccc tgggtaatct gacctttctg
      901 agcttgcagt ggaacatgct tcgggtcctg cctgccggcc tctttgccca caccccgtgc
      961 ctggttggcc tgtctctgac ccataaccag ctggagactg tcgctgaggg cacctttgcc
     1021 cacctgtcca acctgcgttc cctcatgctc tcatacaatg ccattaccca cctcccagct
     1081 ggcatcttca gagacctgga ggagttggtc aaactctacc tgggcagcaa caaccttacg
     1141 gcgctgcacc cagccctctt ccagaacctg tccaagctgg agctgctcag cctctccaag
     1201 aaccagctga ccacacttcc ggagggcatc ttcgacacca actacaacct gttcaacctg
     1261 gccctgcacg gtaacccctg gcagtgcgac tgccacctgg cctacctctt caactggctg
     1321 cagcagtaca ccgatcggct cctgaacatc cagacctact gcgctggccc tgcctacctc
     1381 aaaggccagg tggtgcccgc cttgaatgag aagcagctgg tgtgtcccgt cacccgggac
     1441 cacttgggct tccaggtcac gtggccggac gaaagcaagg cagggggcag ctgggatctg
     1501 gctgtgcagg aaagggcagc ccggagccag tgcacctaca gcaaccccga gggcaccgtg
     1561 gtgctcgcct gtgaccaggc ccagtgtcgc tggctgaacg tccagctctc tcctcagcag
     1621 ggctccctgg gactgcagta caatgctagt caggagtggg acctgaggtc gagctgcggt
     1681 tctctgcggc tcaccgtgtc tatcgaggct cgggcagcag ggccctagta gcagcgcata
     1741 caggagctgg ggaagggggc ctctggggcc tgaccaggcg acaggtaggg gcggagggga
     1801 gctgagtctc cgaagccttg gcttttcaca tgcaagggac agggttacat ccccaaggtg
     1861 agggggtgga gtctggtctg ctccactaac cagggtctcc tcctcctctt ccttcatcgc
     1921 ttctcctgga gtgtgcggcc taacaaggcc atccttatgc cttgcaaagc accctcaaaa
     1981 gctgcaccac agcctggaga ataaaatatc ctcagccctg atgcctcccc attatgtaac
     2041 acccaaccgc tctcacctac accctgaggt ctattcactg catcccagtg atacaaagtg
     2101 gaggccactg ccttctgaca tctggctcaa aagcccagtg tctgtttcca tttatttccc
     2161 tggaatttca tttaaaattg gtatagagaa aaaaaggatg tgacagaagc agagatgacc
     2221 agaaagcaca ggggcagggt tctgactggc gtgtgggaga ccctgtggcc ggcacccacc
     2281 tccacacgag gactaagctc tgattttttt atcttgccca aattcctacc taaggggtct
     2341 agggagtcgc gccttacaaa tcataaattc tcatcagatg ggttttattt gaccctgtat
     2401 atcatgactt atttttaatc tgactatggc ataacattac aagacgaggc aaaaatattt
     2461 aacccccaaa tatatttcct tgccctacct tgaacttgcc ctgcagagtc tcttgtgagg
     2521 agaatccaca tcctataaag aagccccttt cccctttgtt ttccttcctt tctttccagt
     2581 ccaggagatc atcaactaag agccaggcac cccttttaag tcgataagaa acagtttaca
     2641 acctgctctc tctctctctg aagtctgctg agagcttccc ctgcacaata aaacttggcc
     2701 tccacgatcc tttatcttaa cctgaacatt cctttccatt gatcccaggt cttcagctaa
     2761 gctcaaccaa ttgtcaacca gaaaatgttt aaatttacct acagcctgga agcacccacc
     2821 cccgctgctt cgagttgtcc tgcctttctg aactcaacca atgtatttct taaatgtatt
     2881 tgattgatgc ctcattcctc cctaaaatgt ataaaaccaa gctgtacctc gaccaccttg
     2941 ggcacatgtt cccaggccct cctgaggtct gtgtcacggg ccatggccac tcatatttgg
     3001 ctcagaataa atctcttcaa atatt
//
