LOCUS       NM_001308               1855 bp    mRNA    linear   PRI 28-APR-2025
DEFINITION  Homo sapiens carboxypeptidase N subunit 1 (CPN1), mRNA.
ACCESSION   NM_001308
VERSION     NM_001308.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Talens,S., Lebbink,J.H., Malfliet,J.J., Demmers,J.A., Uitte de
            Willige,S., Leebeek,F.W. and Rijken,D.C.
  TITLE     Binding of carboxypeptidase N to fibrinogen and fibrin
  JOURNAL   Biochem Biophys Res Commun 427 (2), 421-425 (2012)
   PUBMED   23000409
  REMARK    GeneRIF: CPN binds to fibrinogen and is present in a fibrin clot
            prepared from plasma.
REFERENCE   3  (bases 1 to 1855)
  AUTHORS   Estrada,K., Styrkarsdottir,U., Evangelou,E., Hsu,Y.H., Duncan,E.L.,
            Ntzani,E.E., Oei,L., Albagha,O.M., Amin,N., Kemp,J.P., Koller,D.L.,
            Li,G., Liu,C.T., Minster,R.L., Moayyeri,A., Vandenput,L.,
            Willner,D., Xiao,S.M., Yerges-Armstrong,L.M., Zheng,H.F.,
            Alonso,N., Eriksson,J., Kammerer,C.M., Kaptoge,S.K., Leo,P.J.,
            Thorleifsson,G., Wilson,S.G., Wilson,J.F., Aalto,V., Alen,M.,
            Aragaki,A.K., Aspelund,T., Center,J.R., Dailiana,Z., Duggan,D.J.,
            Garcia,M., Garcia-Giralt,N., Giroux,S., Hallmans,G., Hocking,L.J.,
            Husted,L.B., Jameson,K.A., Khusainova,R., Kim,G.S., Kooperberg,C.,
            Koromila,T., Kruk,M., Laaksonen,M., Lacroix,A.Z., Lee,S.H.,
            Leung,P.C., Lewis,J.R., Masi,L., Mencej-Bedrac,S., Nguyen,T.V.,
            Nogues,X., Patel,M.S., Prezelj,J., Rose,L.M., Scollen,S.,
            Siggeirsdottir,K., Smith,A.V., Svensson,O., Trompet,S., Trummer,O.,
            van Schoor,N.M., Woo,J., Zhu,K., Balcells,S., Brandi,M.L.,
            Buckley,B.M., Cheng,S., Christiansen,C., Cooper,C., Dedoussis,G.,
            Ford,I., Frost,M., Goltzman,D., Gonzalez-Macias,J., Kahonen,M.,
            Karlsson,M., Khusnutdinova,E., Koh,J.M., Kollia,P., Langdahl,B.L.,
            Leslie,W.D., Lips,P., Ljunggren,O., Lorenc,R.S., Marc,J.,
            Mellstrom,D., Obermayer-Pietsch,B., Olmos,J.M.,
            Pettersson-Kymmer,U., Reid,D.M., Riancho,J.A., Ridker,P.M.,
            Rousseau,F., Slagboom,P.E., Tang,N.L., Urreizti,R., Van Hul,W.,
            Viikari,J., Zarrabeitia,M.T., Aulchenko,Y.S.,
            Castano-Betancourt,M., Grundberg,E., Herrera,L., Ingvarsson,T.,
            Johannsdottir,H., Kwan,T., Li,R., Luben,R., Medina-Gomez,C.,
            Palsson,S.T., Reppe,S., Rotter,J.I., Sigurdsson,G., van Meurs,J.B.,
            Verlaan,D., Williams,F.M., Wood,A.R., Zhou,Y., Gautvik,K.M.,
            Pastinen,T., Raychaudhuri,S., Cauley,J.A., Chasman,D.I.,
            Clark,G.R., Cummings,S.R., Danoy,P., Dennison,E.M., Eastell,R.,
            Eisman,J.A., Gudnason,V., Hofman,A., Jackson,R.D., Jones,G.,
            Jukema,J.W., Khaw,K.T., Lehtimaki,T., Liu,Y., Lorentzon,M.,
            McCloskey,E., Mitchell,B.D., Nandakumar,K., Nicholson,G.C.,
            Oostra,B.A., Peacock,M., Pols,H.A., Prince,R.L., Raitakari,O.,
            Reid,I.R., Robbins,J., Sambrook,P.N., Sham,P.C., Shuldiner,A.R.,
            Tylavsky,F.A., van Duijn,C.M., Wareham,N.J., Cupples,L.A.,
            Econs,M.J., Evans,D.M., Harris,T.B., Kung,A.W., Psaty,B.M.,
            Reeve,J., Spector,T.D., Streeten,E.A., Zillikens,M.C.,
            Thorsteinsdottir,U., Ohlsson,C., Karasik,D., Richards,J.B.,
            Brown,M.A., Stefansson,K., Uitterlinden,A.G., Ralston,S.H.,
            Ioannidis,J.P., Kiel,D.P. and Rivadeneira,F.
  TITLE     Genome-wide meta-analysis identifies 56 bone mineral density loci
            and reveals 14 loci associated with risk of fracture
  JOURNAL   Nat Genet 44 (5), 491-501 (2012)
   PUBMED   22504420
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1855)
  AUTHORS   Chambers,J.C., Zhang,W., Sehmi,J., Li,X., Wass,M.N., Van der
            Harst,P., Holm,H., Sanna,S., Kavousi,M., Baumeister,S.E.,
            Coin,L.J., Deng,G., Gieger,C., Heard-Costa,N.L., Hottenga,J.J.,
            Kuhnel,B., Kumar,V., Lagou,V., Liang,L., Luan,J., Vidal,P.M., Mateo
            Leach,I., O'Reilly,P.F., Peden,J.F., Rahmioglu,N., Soininen,P.,
            Speliotes,E.K., Yuan,X., Thorleifsson,G., Alizadeh,B.Z.,
            Atwood,L.D., Borecki,I.B., Brown,M.J., Charoen,P., Cucca,F.,
            Das,D., de Geus,E.J., Dixon,A.L., Doring,A., Ehret,G.,
            Eyjolfsson,G.I., Farrall,M., Forouhi,N.G., Friedrich,N.,
            Goessling,W., Gudbjartsson,D.F., Harris,T.B., Hartikainen,A.L.,
            Heath,S., Hirschfield,G.M., Hofman,A., Homuth,G., Hypponen,E.,
            Janssen,H.L., Johnson,T., Kangas,A.J., Kema,I.P., Kuhn,J.P.,
            Lai,S., Lathrop,M., Lerch,M.M., Li,Y., Liang,T.J., Lin,J.P.,
            Loos,R.J., Martin,N.G., Moffatt,M.F., Montgomery,G.W., Munroe,P.B.,
            Musunuru,K., Nakamura,Y., O'Donnell,C.J., Olafsson,I.,
            Penninx,B.W., Pouta,A., Prins,B.P., Prokopenko,I., Puls,R.,
            Ruokonen,A., Savolainen,M.J., Schlessinger,D., Schouten,J.N.,
            Seedorf,U., Sen-Chowdhry,S., Siminovitch,K.A., Smit,J.H.,
            Spector,T.D., Tan,W., Teslovich,T.M., Tukiainen,T.,
            Uitterlinden,A.G., Van der Klauw,M.M., Vasan,R.S., Wallace,C.,
            Wallaschofski,H., Wichmann,H.E., Willemsen,G., Wurtz,P., Xu,C.,
            Yerges-Armstrong,L.M., Abecasis,G.R., Ahmadi,K.R., Boomsma,D.I.,
            Caulfield,M., Cookson,W.O., van Duijn,C.M., Froguel,P., Matsuda,K.,
            McCarthy,M.I., Meisinger,C., Mooser,V., Pietilainen,K.H.,
            Schumann,G., Snieder,H., Sternberg,M.J., Stolk,R.P., Thomas,H.C.,
            Thorsteinsdottir,U., Uda,M., Waeber,G., Wareham,N.J.,
            Waterworth,D.M., Watkins,H., Whitfield,J.B., Witteman,J.C.,
            Wolffenbuttel,B.H., Fox,C.S., Ala-Korpela,M., Stefansson,K.,
            Vollenweider,P., Volzke,H., Schadt,E.E., Scott,J., Jarvelin,M.R.,
            Elliott,P. and Kooner,J.S.
  CONSRTM   Alcohol Genome-wide Association (AlcGen) Consortium; Diabetes
            Genetics Replication and Meta-analyses (DIAGRAM+) Study; Genetic
            Investigation of Anthropometric Traits (GIANT) Consortium; Global
            Lipids Genetics Consortium; Genetics of Liver Disease (GOLD)
            Consortium; International Consortium for Blood Pressure
            (ICBP-GWAS); Meta-analyses of Glucose and Insulin-Related Traits
            Consortium (MAGIC)
  TITLE     Genome-wide association study identifies loci influencing
            concentrations of liver enzymes in plasma
  JOURNAL   Nat Genet 43 (11), 1131-1138 (2011)
   PUBMED   22001757
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1855)
  AUTHORS   Grilo,A., Saez-Rosas,M.P., Santos-Morano,J., Sanchez,E.,
            Moreno-Rey,C., Real,L.M., Ramirez-Lorca,R. and Saez,M.E.
  TITLE     Identification of genetic factors associated with susceptibility to
            angiotensin-converting enzyme inhibitors-induced cough
  JOURNAL   Pharmacogenet Genomics 21 (1), 10-17 (2011)
   PUBMED   21052031
  REMARK    GeneRIF: Observational study of gene-disease association, gene-gene
            interaction, gene-environment interaction, and pharmacogenomic /
            toxicogenomic. (HuGE Navigator)
REFERENCE   6  (bases 1 to 1855)
  AUTHORS   Riley,D.A., Tan,F., Miletich,D.J. and Skidgel,R.A.
  TITLE     Chromosomal localization of the genes for human carboxypeptidase D
            (CPD) and the active 50-kilodalton subunit of human
            carboxypeptidase N (CPN1)
  JOURNAL   Genomics 50 (1), 105-108 (1998)
   PUBMED   9628828
REFERENCE   7  (bases 1 to 1855)
  AUTHORS   Hendriks,D., Vingron,M., Vriend,G., Wang,W., Nalis,D. and
            Scharpe,S.
  TITLE     On the specificity of carboxypeptidase N, a comparative study
  JOURNAL   Biol Chem Hoppe Seyler 374 (9), 843-849 (1993)
   PUBMED   8267877
REFERENCE   8  (bases 1 to 1855)
  AUTHORS   Gebhard,W., Schube,M. and Eulitz,M.
  TITLE     cDNA cloning and complete primary structure of the small, active
            subunit of human carboxypeptidase N (kininase 1)
  JOURNAL   Eur J Biochem 178 (3), 603-607 (1989)
   PUBMED   2912725
REFERENCE   9  (bases 1 to 1855)
  AUTHORS   Gebhard,W., Schube,M. and Eulitz,M.
  TITLE     cDNA cloning of kininase 1
  JOURNAL   Adv Exp Med Biol 247B, 261-264 (1989)
   PUBMED   2610070
REFERENCE   10 (bases 1 to 1855)
  AUTHORS   Skidgel,R.A., Bennett,C.D., Schilling,J.W., Tan,F.L.,
            Weerasinghe,D.K. and Erdos,E.G.
  TITLE     Amino acid sequence of the N-terminus and selected tryptic peptides
            of the active subunit of human plasma carboxypeptidase N:
            comparison with other carboxypeptidases
  JOURNAL   Biochem Biophys Res Commun 154 (3), 1323-1329 (1988)
   PUBMED   3408501
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL441886.18, X14329.1 and
            AW950687.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_001308.2.
            
            Summary: Carboxypeptidase N is a plasma metallo-protease that
            cleaves basic amino acids from the C terminal of peptides and
            proteins. The enzyme is important in the regulation of peptides
            like kinins and anaphylatoxins, and has also been known as
            kininase-1 and anaphylatoxin inactivator. This enzyme is a tetramer
            comprised of two identical regulatory subunits and two identical
            catalytic subunits; this gene encodes the catalytic subunit.
            Mutations in this gene can be associated with angioedema or chronic
            urticaria resulting from carboxypeptidase N deficiency. [provided
            by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC027897.1, X14329.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN03465408 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000370418.8/ ENSP00000359446.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-32                AL441886.18        129872-129903       c
            33-1733             X14329.1           2-1702
            1734-1740           AW950687.1         445-451
            1741-1855           AL441886.18        90227-90341         c
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q24.2"
     gene            1..1855
                     /gene="CPN1"
                     /gene_synonym="CPN; SCPN"
                     /note="carboxypeptidase N subunit 1"
                     /db_xref="GeneID:1369"
                     /db_xref="HGNC:HGNC:2312"
                     /db_xref="MIM:603103"
     exon            1..467
                     /gene="CPN1"
                     /gene_synonym="CPN; SCPN"
                     /inference="alignment:Splign:2.1.0"
     CDS             245..1621
                     /gene="CPN1"
                     /gene_synonym="CPN; SCPN"
                     /EC_number="3.4.17.3"
                     /note="carboxypeptidase N catalytic chain; kininase I;
                     anaphylatoxin inactivator; serum carboxypeptidase N;
                     carboxypeptidase N catalytic subunit; carboxypeptidase N
                     small subunit; lysine carboxypeptidase; arginine
                     carboxypeptidase; plasma carboxypeptidase B;
                     carboxypeptidase N polypeptide 1 50 kD; kininase-1;
                     carboxypeptidase K; carboxypeptidase N, polypeptide 1"
                     /codon_start=1
                     /product="carboxypeptidase N catalytic chain precursor"
                     /protein_id="NP_001299.1"
                     /db_xref="CCDS:CCDS7486.1"
                     /db_xref="GeneID:1369"
                     /db_xref="HGNC:HGNC:2312"
                     /db_xref="MIM:603103"
                     /translation="MSDLLSVFLHLLLLFKLVAPVTFRHHRYDDLVRTLYKVQNECPG
                     ITRVYSIGRSVEGRHLYVLEFSDHPGIHEPLEPEVKYVGNMHGNEALGRELMLQLSEF
                     LCEEFRNRNQRIVQLIQDTRIHILPSMNPDGYEVAAAQGPNKPGYLVGRNNANGVDLN
                     RNFPDLNTYIYYNEKYGGPNHHLPLPDNWKSQVEPETRAVIRWMHSFNFVLSANLHGG
                     AVVANYPYDKSFEHRVRGVRRTASTPTPDDKLFQKLAKVYSYAHGWMFQGWNCGDYFP
                     DGITNGASWYSLSKGMQDFNYLHTNCFEITLELSCDKFPPEEELQREWLGNREALIQF
                     LEQVHQGIKGMVLDENYNNLANAVISVSGINHDVTSGDHGDYFRLLLPGIYTVSATAP
                     GYDPETVTVTVGPAEPTLVNFHLKRSIPQVSPVRRAPSRRHGVRAKVQPQARKKEMEM
                     RQLQRGPA"
     exon            468..664
                     /gene="CPN1"
                     /gene_synonym="CPN; SCPN"
                     /inference="alignment:Splign:2.1.0"
     exon            665..820
                     /gene="CPN1"
                     /gene_synonym="CPN; SCPN"
                     /inference="alignment:Splign:2.1.0"
     exon            821..1003
                     /gene="CPN1"
                     /gene_synonym="CPN; SCPN"
                     /inference="alignment:Splign:2.1.0"
     exon            1004..1115
                     /gene="CPN1"
                     /gene_synonym="CPN; SCPN"
                     /inference="alignment:Splign:2.1.0"
     exon            1116..1255
                     /gene="CPN1"
                     /gene_synonym="CPN; SCPN"
                     /inference="alignment:Splign:2.1.0"
     exon            1256..1355
                     /gene="CPN1"
                     /gene_synonym="CPN; SCPN"
                     /inference="alignment:Splign:2.1.0"
     exon            1356..1474
                     /gene="CPN1"
                     /gene_synonym="CPN; SCPN"
                     /inference="alignment:Splign:2.1.0"
     exon            1475..1855
                     /gene="CPN1"
                     /gene_synonym="CPN; SCPN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1717..1722
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CPN1"
                     /gene_synonym="CPN; SCPN"
                     /note="hexamer: AATACA"
     polyA_site      1738
                     /gene="CPN1"
                     /gene_synonym="CPN; SCPN"
                     /note="major polyA site"
ORIGIN      
        1 acctttcaaa aggctttcct ggattagaga aagaaaggga gtgagggagg agagatgagt
       61 ggctattcca gaacgacata aagaatttcc agccttggac ggacagctgg gaacgtcttc
      121 caatttggac tggtgtttac aagcgggaag ctaggtggac cttggatttt ggcgggtgaa
      181 gaggctaggt tgtttaagga ggtggggcgc gtttcagtgg ctctctttga aaaagcccag
      241 caagatgtca gacctgctct cagtcttcct ccacctcctc cttctcttca agttggttgc
      301 cccggtgacc tttcgccacc accgctatga tgatcttgtg cggacgctgt acaaggtgca
      361 aaacgaatgc cccggcatca cgcgggtcta cagcattggg cgcagcgtgg aggggagaca
      421 cctctacgtg ctggagttca gcgaccaccc tggaatccac gagcccttgg aaccagaggt
      481 caagtatgtg gggaacatgc acggcaacga agcgttgggc cgcgagctga tgctgcagct
      541 gtcggagttt ctgtgcgagg agttccggaa caggaaccag cgcatcgtcc agctcatcca
      601 ggacacgcgc attcacatcc tgccatccat gaaccccgac ggctacgagg tggctgctgc
      661 ccagggccca aacaagcctg ggtatctagt tggcaggaac aatgcaaatg gagtggacct
      721 gaaccgcaac ttccctgatc tcaataccta tatctactat aacgagaagt acggaggccc
      781 caaccaccac ctgccccttc cagacaactg gaaaagtcag gtggaacccg agacccgggc
      841 ggtgatccgg tggatgcact ccttcaactt tgttctttca gccaatctcc acggaggggc
      901 ggtggtggcc aattacccgt atgacaagtc ctttgagcac cgggtccgag gggtccgccg
      961 caccgccagc acccccacgc ctgacgacaa gctcttccag aagctggcca aggtctactc
     1021 ctatgcacat ggatggatgt tccaaggttg gaactgcgga gattacttcc cagatggcat
     1081 caccaatggg gcttcctggt attctctcag caagggaatg caagacttta attatctcca
     1141 taccaactgc tttgagatca cgctggaact gagttgcgac aagtttcccc ccgaagagga
     1201 gttacagcgg gagtggctgg gtaatcggga agccctaatc cagttcctgg aacaggttca
     1261 ccagggcatc aagggaatgg tgcttgatga gaattacaat aatctcgcca atgctgtcat
     1321 ttctgtcagt gggattaacc atgatgtcac ttcaggtgac catggtgatt acttccggct
     1381 gctgcttcca ggtatctaca ctgttagtgc cacagcacct gggtatgacc cagagacagt
     1441 aactgtgacc gtgggtcctg cggaaccaac gttggttaac ttccacctca aaagaagcat
     1501 ccctcaagta agccctgtga ggagagctcc cagcagaagg cacggagtca gagccaaagt
     1561 gcagccccaa gccagaaaga aagaaatgga gatgaggcag ctgcagagag gccctgcctg
     1621 aaacccacag tgccaggcaa cccttcagaa aggctttgct cctgctctca gatcagatca
     1681 agcattcttt ctattttatt atctgggaca tatttaaata caaacatatt cagaacaatt
     1741 cagaatggtc tccatctgtt ccaggggtaa gccatctcta ggtcatcatg aaaaaaatta
     1801 ggatctattg aaaggtgatg tggtggccgg gtgtggtggc tcacatctgt aatcc
//
