LOCUS       NM_001008220            4597 bp    mRNA    linear   PRI 24-APR-2024
DEFINITION  Homo sapiens complexin 2 (CPLX2), transcript variant 2, mRNA.
ACCESSION   NM_001008220
VERSION     NM_001008220.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Chen,Y., Ning,J., Shu,L., Wen,L., Yan,B., Wang,Z., Hu,J., Zhou,X.,
            Tao,Y., Xia,X. and Huang,J.
  TITLE     CPLX2 is a novel tumor suppressor and improves the prognosis in
            glioma
  JOURNAL   J Neurooncol 167 (1), 63-74 (2024)
   PUBMED   38427133
  REMARK    GeneRIF: CPLX2 is a novel tumor suppressor and improves the
            prognosis in glioma.
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Li,H., Zhao,J., Zhong,X.L., Xu,P.Y., Du,L.J., Fang,P., Tan,L.J.,
            Li,M.J., Zhang,C.F. and Cao,T.S.
  TITLE     CPLX2 Regulates Ferroptosis and Apoptosis Through NRF2 Pathway in
            Human Hepatocellular Carcinoma Cells
  JOURNAL   Appl Biochem Biotechnol 195 (1), 597-609 (2023)
   PUBMED   36107387
  REMARK    GeneRIF: CPLX2 Regulates Ferroptosis and Apoptosis Through NRF2
            Pathway in Human Hepatocellular Carcinoma Cells.
REFERENCE   3  (bases 1 to 4597)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 4597)
  AUTHORS   Ramos-Miguel,A., Sawada,K., Jones,A.A., Thornton,A.E., Barr,A.M.,
            Leurgans,S.E., Schneider,J.A., Bennett,D.A. and Honer,W.G.
  TITLE     Presynaptic proteins complexin-I and complexin-II differentially
            influence cognitive function in early and late stages of
            Alzheimer's disease
  JOURNAL   Acta Neuropathol 133 (3), 395-407 (2017)
   PUBMED   27866231
  REMARK    GeneRIF: This study demonstrated that complexin-II differentially
            influence cognitive function in early and late stages of
            Alzheimer's disease.
REFERENCE   5  (bases 1 to 4597)
  AUTHORS   Kurokawa,A., Narukawa,M., Ohmoto,M., Yoshimoto,J., Abe,K. and
            Misaka,T.
  TITLE     Expression of the synaptic exocytosis-regulating molecule complexin
            2 in taste buds and its participation in peripheral taste
            transduction
  JOURNAL   J Neurochem 133 (6), 806-814 (2015)
   PUBMED   25692331
  REMARK    GeneRIF: These results suggested that CPLX2 participated in
            synaptic taste transduction.
REFERENCE   6  (bases 1 to 4597)
  AUTHORS   Yamada,M., Saisu,H., Ishizuka,T., Takahashi,H. and Abe,T.
  TITLE     Immunohistochemical distribution of the two isoforms of
            synaphin/complexin involved in neurotransmitter release:
            localization at the distinct central nervous system regions and
            synaptic types
  JOURNAL   Neuroscience 93 (1), 7-18 (1999)
   PUBMED   10430466
REFERENCE   7  (bases 1 to 4597)
  AUTHORS   Harrison,P.J. and Eastwood,S.L.
  TITLE     Preferential involvement of excitatory neurons in medial temporal
            lobe in schizophrenia
  JOURNAL   Lancet 352 (9141), 1669-1673 (1998)
   PUBMED   9853440
  REMARK    Erratum:[Lancet 1999 Jan 9;353(9147):154]
REFERENCE   8  (bases 1 to 4597)
  AUTHORS   Morgans,C.W., Brandstatter,J.H., Kellerman,J., Betz,H. and
            Wassle,H.
  TITLE     A SNARE complex containing syntaxin 3 is present in ribbon synapses
            of the retina
  JOURNAL   J Neurosci 16 (21), 6713-6721 (1996)
   PUBMED   8824312
REFERENCE   9  (bases 1 to 4597)
  AUTHORS   McMahon,H.T., Missler,M., Li,C. and Sudhof,T.C.
  TITLE     Complexins: cytosolic proteins that regulate SNAP receptor function
  JOURNAL   Cell 83 (1), 111-119 (1995)
   PUBMED   7553862
REFERENCE   10 (bases 1 to 4597)
  AUTHORS   Christensen,V.L. and Johnston,N.P.
  TITLE     Effect of time of day of insemination and the position of the egg
            in the oviduct on the fertility of turkeys
  JOURNAL   Poult Sci 56 (2), 458-462 (1977)
   PUBMED   605033
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AY576870.1, U35100.1,
            BI197868.1, AK124837.1 and AC138965.2.
            
            On Nov 22, 2018 this sequence version replaced NM_001008220.1.
            
            Summary: Proteins encoded by the complexin/synaphin gene family are
            cytosolic proteins that function in synaptic vesicle exocytosis.
            These proteins bind syntaxin, part of the SNAP receptor. The
            protein product of this gene binds to the SNAP receptor complex and
            disrupts it, allowing transmitter release. Two transcript variants
            encoding the same protein have been found for this gene. [provided
            by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 and 2 both encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BN000500.2, SRR1803617.207302.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2142348
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000393745.8/ ENSP00000377346.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-560               AY576870.1         81-640
            561-804             U35100.1           690-933
            805-834             BI197868.1         10-39
            835-864             AK124837.1         66-95
            865-883             BI197868.1         72-90
            884-4596            AK124837.1         115-3827
            4597-4597           AC138965.2         67448-67448
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q35.2"
     gene            1..4597
                     /gene="CPLX2"
                     /gene_synonym="921-L; CPX-2; CPX2; Hfb1"
                     /note="complexin 2"
                     /db_xref="GeneID:10814"
                     /db_xref="HGNC:HGNC:2310"
                     /db_xref="MIM:605033"
     exon            1..128
                     /gene="CPLX2"
                     /gene_synonym="921-L; CPX-2; CPX2; Hfb1"
                     /inference="alignment:Splign:2.1.0"
     exon            129..247
                     /gene="CPLX2"
                     /gene_synonym="921-L; CPX-2; CPX2; Hfb1"
                     /inference="alignment:Splign:2.1.0"
     CDS             217..621
                     /gene="CPLX2"
                     /gene_synonym="921-L; CPX-2; CPX2; Hfb1"
                     /note="synaphin 1; CPX II; complexin II"
                     /codon_start=1
                     /product="complexin-2"
                     /protein_id="NP_001008221.1"
                     /db_xref="CCDS:CCDS4396.1"
                     /db_xref="GeneID:10814"
                     /db_xref="HGNC:HGNC:2310"
                     /db_xref="MIM:605033"
                     /translation="MDFVMKQALGGATKDMGKMLGGEEEKDPDAQKKEEERQEALRQQ
                     EEERKAKHARMEAEREKVRQQIRDKYGLKKKEEKEAEEKAALEQPCEGSLTRPKKAIP
                     AGCGDEEEEEEESILDTVLKYLPGPLQDMFKK"
     exon            248..423
                     /gene="CPLX2"
                     /gene_synonym="921-L; CPX-2; CPX2; Hfb1"
                     /inference="alignment:Splign:2.1.0"
     exon            424..4597
                     /gene="CPLX2"
                     /gene_synonym="921-L; CPX-2; CPX2; Hfb1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4576..4581
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CPLX2"
                     /gene_synonym="921-L; CPX-2; CPX2; Hfb1"
                     /note="hexamer: AAGAAA"
     polyA_site      4597
                     /gene="CPLX2"
                     /gene_synonym="921-L; CPX-2; CPX2; Hfb1"
                     /note="major polyA site"
ORIGIN      
        1 agaagccgcg gctccagctc gcctggcgga attgcacgcg gcggcgggag ctggaatagc
       61 agaaggaacc acctcgtgga gtcgggccgg agccctgcag tggctcagac ggttgcaggg
      121 accgccaggt tgtcacatct tcccaagcca ggccagccag gagcgctgca tgcaaattct
      181 gccgtgggct aaggcacgct aaccagagcc ggcggcatgg acttcgtcat gaagcaggcc
      241 cttggagggg ccacaaagga catggggaag atgctggggg gagaggagga gaaggacccc
      301 gacgcgcaga aaaaggagga ggagcggcag gaggcgctgc ggcagcagga ggaggagcgt
      361 aaggccaagc acgcgcgcat ggaggcggag cgggagaagg tccggcagca gatccgagat
      421 aagtatgggc tgaagaagaa ggaggagaag gaagcagagg agaaagcagc cctggagcag
      481 ccctgcgagg ggagcctgac ccggcccaag aaggccatcc ctgcgggctg cggggacgag
      541 gaggaggagg aagaggagag catcctggac acggtgctca aatacctgcc cgggccgctg
      601 caggacatgt tcaagaagta accaggcctc ctgccccagc ctactccacc tgttactact
      661 tctttttggt tctttctttt ctttttatta ggttaagtct caattctgaa ggggaaaacc
      721 tcagttggcc tctgcccctc ttccctggcc aggggcttct ccccctcagc tctccctcac
      781 acctcccttc atcccagggt atccacctgc accccactcc caagtagctt gaaaaaggga
      841 ggacagtctt tccccagcag gggtcagggg ggcccctcag gaagcctaag gtcgtgctag
      901 tgtggtgacc cccatacatt cctccctgct ccccactgcc aggaggacca ctgtccccag
      961 ccagccaaag taatgacaca ttccagccct gcccagcatg ctgacctttg gcctctaacc
     1021 ctcagtgggc ccccaggtca gggcaggggc actgagtggc ctggctctga ggaagggagt
     1081 caggggaagc ctgtcccggg aaggcccagg ctgagaggcc ctggctctgg ccaggctggg
     1141 atctgggtgg gaggctgggg ctcttcttct tccatctcct tggtgacacc cagcccaggg
     1201 gcaccccctt ccccagcccc cacctggaga gacatggccc ctgccaagct ggtcccttca
     1261 aatggatcct ttgtggactt tagctcattt gtggaggaac cccaggtagg gacgcccctt
     1321 gttcctcacc cccaccccac ttaggtcctg ggcccccact gccaggctgg gcccagcttg
     1381 ctcagtcaag gggctgccag gcccccagaa aacacttgga gccatcgggt agcgatggtc
     1441 tatgccatgg ggaacacctc cattggtgtg gccaagctgc ccccattcct atccacccct
     1501 ctccccaccc cgtcctgtcc atgcgcttcc agggccccac ggtccccagg aggacgcttc
     1561 ctggccaaag ccccaagcct ttggtgagaa gccaattccc acttgacaga aggcgtccat
     1621 ccattcatct cattggccaa ggacaaactc tcctctggga cgtctgggac tggcatttgt
     1681 cccccactca aattatcaaa gctttctgct cagtcagttg tgtggggatg gtgagggaag
     1741 aggggtcaca tgagggagga aactgtatcc atgcatgcat gataatgcgt ggcagagact
     1801 gcaacaggga ttgtgtgttc agagatcata tgcatatgtg tagggctgga gcgtgtgtgt
     1861 gtcttgagat tgtgtgtgtt gcagtcatca tatctatgtg ttacagattg tgtatgttag
     1921 ccttgtgtat gtgtgcttga ttgaggtggt gtatttgggt tgaaattgtg tcatatgtgt
     1981 gtgctatcca tctcgtgttt agaggctgta tatgttagct tgtgtaagaa tgtgttttca
     2041 aaacagtgtg tgtattggga gtgatgggta tgtgttaggt atgtgatggg ttgtagaagc
     2101 gtgtgtttga gagaattcag agacatttga aggctgctgt gtgcatgttt gggggtctga
     2161 aaagacagtt gtgtgcatgg atgtgtgcgt ggggagaaag aacgtgggta agatgtccct
     2221 tcccagccct gagaccactg gtcacagttg gccacctcca acgggagacc ttgtccttgg
     2281 cctagagtcc tcccaccctt ggggggctcc tgcctgaggt cctcagaatc ccactgcaat
     2341 ggacccaggc agcgccccag gaagccatgc tgggcccccg ccagggccta tcccaaaagc
     2401 aggggccagg gagggggcga cttgcctgcc cctgaagccc ttgttcccat tggccccagt
     2461 ttgcattctg caggttttcc attttagtgg gttctgcttt tatttcagag acagacatgt
     2521 gtcttctctg tccgtttcca ataggtaaag ccatatcagt tagactgcaa tactttaaac
     2581 acgagacaaa acaatccata tgtttaggga accagaaaag tcccctggtc tgtcccttct
     2641 ttggggagca gggcctcgac agctccagct cccttgacct accttcctcc ccgcaccccg
     2701 cccccacctt gtgcccctgt gtccagcccc ccagggggcc tgtgtctgtg tctgtgcctg
     2761 tgtctgtgat ggggagccgc ctcgcacccc tgttgtctgc ttgtctcttt gtgtctgtta
     2821 tcctgggcag gatggtcatt ctcaaaaacc ctggggtcct gggccagaga caggcagggc
     2881 ccagtccagg ggccccaggc ctccccagtc ccagtgtgcg agccccactt ggacacaagt
     2941 gttcagagag gtccccctct gccacttgac agggaccttc aaacctcgac agtgatgcaa
     3001 ggacacagag agtaccagat aggtagcaga gaccaaggcg cagggtgctt cagatgagca
     3061 agagaaccca gtcgaaccag ataccccagg tgggccggag ggaccccaga ccttcagagg
     3121 gctgccctgg tgttctccac agtgcagtcc ctctgtattc ccagagtggg atcggggctt
     3181 tcagccccac cctgatgcct gccctccagg atggctggtt tagtctgggt ccatgtccca
     3241 gacccctcta ttctgctcca ggacagcagg acttcaggtc ttcctggggg tggatatagg
     3301 agaaaatttc tgcctggcac acacctggct ccaaccactg ccaagtgatc actcttaggc
     3361 ccaggggaac acaatgacta tcattactga tgcagacctg gctgtggaga gcagctaatg
     3421 tgtggcccag agagcctgtc tgtgtggagc acgtagtgca cagaatacgt gagagttgct
     3481 ctggcagggg cagaatcctc acaggatcgc ctgggaggtg aggtgtgtgt gacccactgg
     3541 atgggagggc aatgagtgtg cacatacaaa tggggcagtg tgcatgcaac acacttaggg
     3601 gaggagtggc cccagaattc agcacgcaca caacacacaa gggagagaac ccccagatga
     3661 gaaaatagga aggagcaatc atttgtagat gggtgaaaaa agaatgaggt tcaagggagc
     3721 gtgcaccagg tgaggtgagc gtgtgtgctc tcagggaagg gcccaggatc ccatgcctgg
     3781 gaggagctgc cagagagaag caaaaaggcg gctgtggatc gccctgggct gggcaccagt
     3841 gacaggtcag gatctccaaa catggacgtc ctcccctcca aatccagaag ctcccagaag
     3901 gtgtccttaa ctgcaaagct gtgcagggta ctcctccaga tggaatcagg aagtcgagac
     3961 accatcccag gtgtgtgtaa gagagagaga gagaacaggg aggatacaga agtattgcag
     4021 cccagatccc ctatcagggg gacagctggt gggcaaagca gccaccccac agccttgtgg
     4081 ctagagtaca gtggggtaga ccctccagcc ccaatagccc tagtacccag ctggcagggt
     4141 tgcccacccc tgctgtccac ctgctccatc ctctagggtt ccacaggccc ctgaccgcac
     4201 agggaggctg gggccagcct ggtctcccag gcctgaggac atgcctccca ccaaatgtcc
     4261 cctgctccag tcccactcct gtcaccccac gctctgcact ggggagaaaa cgggaggtgc
     4321 tcgtgctggc cctgggtggg agcggggagt cctggtgaga ccccggtgag atggaccatc
     4381 ctgcccccgt gggggatccc ctttcccaca tccgtgctgt gtcattgttg ctctgcttcc
     4441 tttcaatgtg tcagtgcctg gggggagggg aggagcaccc cctcagcccc cctgaacctg
     4501 accaaaagcc atggctgttg ctcccccctt tgtatgatgc aaatgctgaa atgtacaaaa
     4561 tcaaccatga caacaaagaa aaagaccttg tacagca
//
