LOCUS       NM_001872               1724 bp    mRNA    linear   PRI 02-MAY-2024
DEFINITION  Homo sapiens carboxypeptidase B2 (CPB2), transcript variant 1,
            mRNA.
ACCESSION   NM_001872
VERSION     NM_001872.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Hamdy,M., Shaheen,I.A., Khallaf,M. and Selim,Y.M.M.
  TITLE     Thrombin activatable fibrinolysis inhibitor plasma levels and TAFI
            Thr325Ile genetic polymorphism in a cohort of Egyptian sickle cell
            disease patients and impact on disease severity
  JOURNAL   Pediatr Blood Cancer 71 (6), e30959 (2024)
   PUBMED   38520679
  REMARK    GeneRIF: Thrombin activatable fibrinolysis inhibitor plasma levels
            and TAFI Thr325Ile genetic polymorphism in a cohort of Egyptian
            sickle cell disease patients and impact on disease severity.
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Altin,N., Tiglioglu,P., Ulusoy,T.U., Aydin,F.N., Kar,I., Karakoc,B.
            and Utebey,G.
  TITLE     A challenging issue in COVID-19 infection: The relationship between
            PA1-1 and TAFI levels in patients with coagulation disorder: A
            retrospective and observational study
  JOURNAL   Medicine (Baltimore) 103 (15), e37802 (2024)
   PUBMED   38608056
  REMARK    GeneRIF: A challenging issue in COVID-19 infection: The
            relationship between PA1-1 and TAFI levels in patients with
            coagulation disorder: A retrospective and observational study.
REFERENCE   3  (bases 1 to 1724)
  AUTHORS   Wang,Y., Cheng,W., Chen,X., Cheng,C., Zhang,L. and Huang,W.
  TITLE     Serum Proteomics Identified TAFI as a Potential Molecule
            Facilitating the Migration of Peripheral Monocytes to Damaged White
            Matter During Chronic Cerebral Hypoperfusion
  JOURNAL   Neurochem Res 49 (3), 597-616 (2024)
   PUBMED   37978153
  REMARK    GeneRIF: Serum Proteomics Identified TAFI as a Potential Molecule
            Facilitating the Migration of Peripheral Monocytes to Damaged White
            Matter During Chronic Cerebral Hypoperfusion.
REFERENCE   4  (bases 1 to 1724)
  AUTHORS   Huang,Q., Feng,D., Pan,L., Wang,H., Wu,Y., Zhong,B., Gong,J.,
            Lin,H. and Fei,X.
  TITLE     Plasma thrombin-activatable fibrinolysis inhibitor and the 1040C/T
            polymorphism are risk factors for diabetic kidney disease in
            Chinese patients with type 2 diabetes
  JOURNAL   PeerJ 11, e16352 (2023)
   PUBMED   38025709
  REMARK    GeneRIF: Plasma thrombin-activatable fibrinolysis inhibitor and the
            1040C/T polymorphism are risk factors for diabetic kidney disease
            in Chinese patients with type 2 diabetes.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1724)
  AUTHORS   Claesen,K., De Loose,J., Van Wielendaele,P., De Bruyn,E., Sim,Y.,
            Thys,S., De Meester,I. and Hendriks,D.
  TITLE     ProCPU Is Expressed by (Primary) Human Monocytes and Macrophages
            and Expression Differs between States of Differentiation and
            Activation
  JOURNAL   Int J Mol Sci 24 (4), 3725 (2023)
   PUBMED   36835137
  REMARK    GeneRIF: ProCPU Is Expressed by (Primary) Human Monocytes and
            Macrophages and Expression Differs between States of
            Differentiation and Activation.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1724)
  AUTHORS   Valnickova,Z., Thogersen,I.B., Christensen,S., Chu,C.T., Pizzo,S.V.
            and Enghild,J.J.
  TITLE     Activated human plasma carboxypeptidase B is retained in the blood
            by binding to alpha2-macroglobulin and pregnancy zone protein
  JOURNAL   J Biol Chem 271 (22), 12937-12943 (1996)
   PUBMED   8662763
REFERENCE   7  (bases 1 to 1724)
  AUTHORS   Tsai,S.P. and Drayna,D.
  TITLE     The gene encoding human plasma carboxypeptidase B (CPB2) resides on
            chromosome 13
  JOURNAL   Genomics 14 (2), 549-550 (1992)
   PUBMED   1427879
REFERENCE   8  (bases 1 to 1724)
  AUTHORS   Eaton,D.L., Malloy,B.E., Tsai,S.P., Henzel,W. and Drayna,D.
  TITLE     Isolation, molecular cloning, and partial characterization of a
            novel carboxypeptidase B from human plasma
  JOURNAL   J Biol Chem 266 (32), 21833-21838 (1991)
   PUBMED   1939207
REFERENCE   9  (bases 1 to 1724)
  AUTHORS   Pascual,R., Burgos,F.J., Salva,M., Soriano,F., Mendez,E. and
            Aviles,F.X.
  TITLE     Purification and properties of five different forms of human
            procarboxypeptidases
  JOURNAL   Eur J Biochem 179 (3), 609-616 (1989)
   PUBMED   2920728
REFERENCE   10 (bases 1 to 1724)
  AUTHORS   Marinkovic,D.V., Marinkovic,J.N., Erdos,E.G. and Robinson,C.J.
  TITLE     Purification of carboxypeptidase B from human pancreas
  JOURNAL   Biochem J 163 (2), 253-260 (1977)
   PUBMED   17398
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC007057.1, AL137141.10 and
            AL157758.10.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001872.4.
            
            Summary: Carboxypeptidases are enzymes that hydrolyze C-terminal
            peptide bonds. The carboxypeptidase family includes metallo-,
            serine, and cysteine carboxypeptidases. According to their
            substrate specificity, these enzymes are referred to as
            carboxypeptidase A (cleaving aliphatic residues) or
            carboxypeptidase B (cleaving basic amino residues). The protein
            encoded by this gene is activated by trypsin and acts on
            carboxypeptidase B substrates. After thrombin activation, the
            mature protein downregulates fibrinolysis. Polymorphisms have been
            described for this gene and its promoter region. Alternate splicing
            results in multiple transcript variants. [provided by RefSeq, Jun
            2013].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189664.112617.1,
                                           SRR5189664.138637.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN04284274 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000181383.10/ ENSP00000181383.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-314               BC007057.1         1-314
            315-315             AL137141.10        169696-169696
            316-686             BC007057.1         316-686
            687-687             AL157758.10        53794-53794         c
            688-1063            BC007057.1         688-1063
            1064-1064           AL157758.10        42257-42257         c
            1065-1367           BC007057.1         1065-1367
            1368-1724           AL157758.10        39634-39990         c
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q14.13"
     gene            1..1724
                     /gene="CPB2"
                     /gene_synonym="CPU; PCPB; TAFI"
                     /note="carboxypeptidase B2"
                     /db_xref="GeneID:1361"
                     /db_xref="HGNC:HGNC:2300"
                     /db_xref="MIM:603101"
     exon            1..98
                     /gene="CPB2"
                     /gene_synonym="CPU; PCPB; TAFI"
                     /inference="alignment:Splign:2.1.0"
     CDS             25..1296
                     /gene="CPB2"
                     /gene_synonym="CPU; PCPB; TAFI"
                     /EC_number="3.4.17.20"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; carboxypeptidase B2 (plasma, carboxypeptidase
                     U); thrombin-activable fibrinolysis inhibitor;
                     thrombin-activatable fibrinolysis inhibitor;
                     carboxypeptidase B2 (plasma); carboxypeptidase B-like
                     protein; carboxypeptidase R"
                     /codon_start=1
                     /product="carboxypeptidase B2 isoform 1 preproprotein"
                     /protein_id="NP_001863.3"
                     /db_xref="CCDS:CCDS9401.1"
                     /db_xref="GeneID:1361"
                     /db_xref="HGNC:HGNC:2300"
                     /db_xref="MIM:603101"
                     /translation="MKLCSLAVLVPIVLFCEQHVFAFQSGQVLAALPRTSRQVQVLQN
                     LTTTYEIVLWQPVTADLIVKKKQVHFFVNASDVDNVKAHLNVSGIPCSVLLADVEDLI
                     QQQISNDTVSPRASASYYEQYHSLNEIYSWIEFITERHPDMLTKIHIGSSFEKYPLYV
                     LKVSGKEQAAKNAIWIDCGIHAREWISPAFCLWFIGHITQFYGIIGQYTNLLRLVDFY
                     VMPVVNVDGYDYSWKKNRMWRKNRSFYANNHCIGTDLNRNFASKHWCEEGASSSSCSE
                     TYCGLYPESEPEVKAVASFLRRNINQIKAYISMHSYSQHIVFPYSYTRSKSKDHEELS
                     LVASEAVRAIEKISKNTRYTHGHGSETLYLAPGGGDDWIYDLGIKYSFTIELRDTGTY
                     GFLLPERYIKPTCREAFAAVSKIAWHVIRNV"
     exon            99..174
                     /gene="CPB2"
                     /gene_synonym="CPU; PCPB; TAFI"
                     /inference="alignment:Splign:2.1.0"
     exon            175..299
                     /gene="CPB2"
                     /gene_synonym="CPU; PCPB; TAFI"
                     /inference="alignment:Splign:2.1.0"
     exon            300..408
                     /gene="CPB2"
                     /gene_synonym="CPU; PCPB; TAFI"
                     /inference="alignment:Splign:2.1.0"
     exon            409..510
                     /gene="CPB2"
                     /gene_synonym="CPU; PCPB; TAFI"
                     /inference="alignment:Splign:2.1.0"
     exon            511..615
                     /gene="CPB2"
                     /gene_synonym="CPU; PCPB; TAFI"
                     /inference="alignment:Splign:2.1.0"
     exon            616..726
                     /gene="CPB2"
                     /gene_synonym="CPU; PCPB; TAFI"
                     /inference="alignment:Splign:2.1.0"
     exon            727..820
                     /gene="CPB2"
                     /gene_synonym="CPU; PCPB; TAFI"
                     /inference="alignment:Splign:2.1.0"
     exon            821..1023
                     /gene="CPB2"
                     /gene_synonym="CPU; PCPB; TAFI"
                     /inference="alignment:Splign:2.1.0"
     exon            1024..1111
                     /gene="CPB2"
                     /gene_synonym="CPU; PCPB; TAFI"
                     /inference="alignment:Splign:2.1.0"
     exon            1112..1724
                     /gene="CPB2"
                     /gene_synonym="CPU; PCPB; TAFI"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1692..1697
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CPB2"
                     /gene_synonym="CPU; PCPB; TAFI"
                     /note="hexamer: AATAAA"
     polyA_site      1724
                     /gene="CPB2"
                     /gene_synonym="CPU; PCPB; TAFI"
                     /note="major polyA site"
ORIGIN      
        1 gttgtacaga aaattgctgt tgggatgaag ctttgcagcc ttgcagtcct tgtacccatt
       61 gttctcttct gtgagcagca tgtcttcgcg tttcagagtg gccaagttct agctgctctt
      121 cctagaacct ctaggcaagt tcaagttcta cagaatctta ctacaacata tgagattgtt
      181 ctctggcagc cggtaacagc tgaccttatt gtgaagaaaa aacaagtcca tttttttgta
      241 aatgcatctg atgtcgacaa tgtgaaagcc catttaaatg tgagcggaat tccatgcagt
      301 gtcttgctgg cagatgtgga agatcttatt caacagcaga tttccaacga cacagtcagc
      361 ccccgagcct ccgcatcgta ctatgaacag tatcactcac taaatgaaat ctattcttgg
      421 atagaattta taactgagag gcatcctgat atgcttacaa aaatccacat tggatcctca
      481 tttgagaagt acccactcta tgttttaaag gtttctggaa aagaacaagc agccaaaaat
      541 gccatatgga ttgactgtgg aatccatgcc agagaatgga tctctcctgc tttctgcttg
      601 tggttcatag gccatataac tcaattctat gggataatag ggcaatatac caatctcctg
      661 aggcttgtgg atttctatgt tatgccagtg gttaatgtgg atggttatga ctactcatgg
      721 aaaaagaatc gaatgtggag aaagaaccgt tctttctatg cgaacaatca ttgcatcgga
      781 acagacctga ataggaactt tgcttccaaa cactggtgtg aggaaggtgc atccagttcc
      841 tcatgctcgg aaacctactg tggactttat cctgagtcag aaccagaagt gaaggcagtg
      901 gctagtttct tgagaagaaa tatcaaccag attaaagcat acatcagcat gcattcatac
      961 tcccagcata tagtgtttcc atattcctat acacgaagta aaagcaaaga ccatgaggaa
     1021 ctgtctctag tagccagtga agcagttcgt gctattgaga aaattagtaa aaataccagg
     1081 tatacacatg gccatggctc agaaacctta tacctagctc ctggaggtgg ggacgattgg
     1141 atctatgatt tgggcatcaa atattcgttt acaattgaac ttcgagatac gggcacatac
     1201 ggattcttgc tgccggagcg ttacatcaaa cccacctgta gagaagcttt tgccgctgtc
     1261 tctaaaatag cttggcatgt cattaggaat gtttaatgcc cctgatttta tcattctgct
     1321 tccgtatttt aatttactga ttccagcaag accaaatcat tgtatcaaat tatttttaag
     1381 ttttatccgt agttttgata aaagattttc ctattccttg gttctgtcag agaacctaat
     1441 aagtgctact ttgccattaa ggcagactag ggttcatgtc tttttaccct ttaaaaaaaa
     1501 ttgtaaaagt ctagttacct actttttctt tgattttcga cgtttgacta gccatctcaa
     1561 gcaagtttcg acgtttgact agccatctca agcaagttta atcaatgatc atctcacgct
     1621 gatcattgga tcctactcaa caaaaggaag ggtggtcaga agtacattaa agatttctgc
     1681 tccaaatttt caataaattt ctgcttgtgc ctttagaaat acaa
//
