LOCUS       NM_001871               1452 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens carboxypeptidase B1 (CPB1), mRNA.
ACCESSION   NM_001871
VERSION     NM_001871.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Kawamoto,M., Kohi,S., Abe,T., Dbouk,M., Macgregor-Das,A., Koi,C.,
            Song,K.B., Borges,M., Sugimine,R., Laheru,D., Hruban,R.H.,
            Roberts,N., Klein,A.P. and Goggins,M.
  TITLE     Endoplasmic stress-inducing variants in CPB1 and CPA1 and risk of
            pancreatic cancer: A case-control study and meta-analysis
  JOURNAL   Int J Cancer 150 (7), 1123-1133 (2022)
   PUBMED   34817877
  REMARK    GeneRIF: Endoplasmic stress-inducing variants in CPB1 and CPA1 and
            risk of pancreatic cancer: A case-control study and meta-analysis.
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 1452)
  AUTHORS   Tamura,K., Yu,J., Hata,T., Suenaga,M., Shindo,K., Abe,T.,
            MacGregor-Das,A., Borges,M., Wolfgang,C.L., Weiss,M.J., He,J.,
            Canto,M.I., Petersen,G.M., Gallinger,S., Syngal,S., Brand,R.E.,
            Rustgi,A., Olson,S.H., Stoffel,E., Cote,M.L., Zogopoulos,G.,
            Potash,J.B., Goes,F.S., McCombie,R.W., Zandi,P.P., Pirooznia,M.,
            Kramer,M., Parla,J., Eshleman,J.R., Roberts,N.J., Hruban,R.H.,
            Klein,A.P. and Goggins,M.
  TITLE     Mutations in the pancreatic secretory enzymes CPA1 and CPB1 are
            associated with pancreatic cancer
  JOURNAL   Proc Natl Acad Sci U S A 115 (18), 4767-4772 (2018)
   PUBMED   29669919
  REMARK    GeneRIF: study indicates ER stress-inducing variants in CPB1 and
            CPA1 are associated with pancreatic cancer susceptibility and
            implicate ER stress in pancreatic acinar cells in pancreatic cancer
            development.
REFERENCE   4  (bases 1 to 1452)
  AUTHORS   Nakano,E., Geisz,A., Masamune,A., Niihori,T., Hamada,S., Kume,K.,
            Kakuta,Y., Aoki,Y., Matsubara,Y., Ebert,K., Ludwig,M., Braun,M.,
            Groneberg,D.A., Shimosegawa,T., Sahin-Toth,M. and Witt,H.
  TITLE     Variants in pancreatic carboxypeptidase genes CPA2 and CPB1 are not
            associated with chronic pancreatitis
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 309 (8), G688-G694 (2015)
   PUBMED   26316592
  REMARK    GeneRIF: This study showed that CPA2 and CPB1 variants are not
            associated with chronic pancreatitis.
REFERENCE   5  (bases 1 to 1452)
  AUTHORS   Bouchal,P., Dvorakova,M., Roumeliotis,T., Bortlicek,Z.,
            Ihnatova,I., Prochazkova,I., Ho,J.T., Maryas,J., Imrichova,H.,
            Budinska,E., Vyzula,R., Garbis,S.D., Vojtesek,B. and Nenutil,R.
  TITLE     Combined Proteomics and Transcriptomics Identifies Carboxypeptidase
            B1 and Nuclear Factor kappaB (NF-kappaB) Associated Proteins as
            Putative Biomarkers of Metastasis in Low Grade Breast Cancer
  JOURNAL   Mol Cell Proteomics 14 (7), 1814-1830 (2015)
   PUBMED   25903579
  REMARK    GeneRIF: These findings indicate unique pro-metastatic mechanisms
            in grade 1 tumors that can include up-regulation of CPB1,
            activation of NF-kappaB pathway and changes in cell survival and
            cytoskeleton.
REFERENCE   6  (bases 1 to 1452)
  AUTHORS   Yamamoto,K.K., Pousette,A., Chow,P., Wilson,H., el Shami,S. and
            French,C.K.
  TITLE     Isolation of a cDNA encoding a human serum marker for acute
            pancreatitis. Identification of pancreas-specific protein as
            pancreatic procarboxypeptidase B
  JOURNAL   J Biol Chem 267 (4), 2575-2581 (1992)
   PUBMED   1370825
REFERENCE   7  (bases 1 to 1452)
  AUTHORS   Moulard,M., Michon,T., Kerfelec,B. and Chapus,C.
  TITLE     Further studies on the human pancreatic binary complexes involving
            procarboxypeptidase A
  JOURNAL   FEBS Lett 261 (1), 179-183 (1990)
   PUBMED   2307232
REFERENCE   8  (bases 1 to 1452)
  AUTHORS   Pascual,R., Burgos,F.J., Salva,M., Soriano,F., Mendez,E. and
            Aviles,F.X.
  TITLE     Purification and properties of five different forms of human
            procarboxypeptidases
  JOURNAL   Eur J Biochem 179 (3), 609-616 (1989)
   PUBMED   2920728
REFERENCE   9  (bases 1 to 1452)
  AUTHORS   Pousette,A., Fernstad,R., Skoldefors,H. and Carlstrom,K.
  TITLE     A novel serum assay for pancreatic cellular damage. II. High tissue
            specificity of a pancreatic protein
  JOURNAL   Pancreas 3 (6), 642-645 (1988)
   PUBMED   3222247
REFERENCE   10 (bases 1 to 1452)
  AUTHORS   Marinkovic,D.V., Marinkovic,J.N., Erdos,E.G. and Robinson,C.J.
  TITLE     Purification of carboxypeptidase B from human pancreas
  JOURNAL   Biochem J 163 (2), 253-260 (1977)
   PUBMED   17398
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BI770145.1 and BC015338.1.
            
            On Nov 24, 2018 this sequence version replaced NM_001871.2.
            
            Summary: Three different procarboxypeptidases A and two different
            procarboxypeptidases B have been isolated. The B1 and B2 forms
            differ from each other mainly in isoelectric point.
            Carboxypeptidase B1 is a highly tissue-specific protein and is a
            useful serum marker for acute pancreatitis and dysfunction of
            pancreatic transplants. It is not elevated in pancreatic carcinoma.
            [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.55850.1, M81057.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000282957.9/ ENSP00000282957.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4                 BI770145.1         11-14
            5-26                BI770145.1         16-37
            27-1452             BC015338.1         18-1443
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q24"
     gene            1..1452
                     /gene="CPB1"
                     /gene_synonym="CPB; PASP; PCPB"
                     /note="carboxypeptidase B1"
                     /db_xref="GeneID:1360"
                     /db_xref="HGNC:HGNC:2299"
                     /db_xref="MIM:114852"
     exon            1..84
                     /gene="CPB1"
                     /gene_synonym="CPB; PASP; PCPB"
                     /inference="alignment:Splign:2.1.0"
     CDS             14..1267
                     /gene="CPB1"
                     /gene_synonym="CPB; PASP; PCPB"
                     /EC_number="3.4.17.2"
                     /note="procarboxypeptidase B; pancreas-specific protein;
                     protaminase; carboxypeptidase B; tissue carboxypeptidase
                     B; pancreatic carboxypeptidase B; carboxypeptidase B1
                     (tissue)"
                     /codon_start=1
                     /product="carboxypeptidase B preproprotein"
                     /protein_id="NP_001862.2"
                     /db_xref="CCDS:CCDS33874.1"
                     /db_xref="GeneID:1360"
                     /db_xref="HGNC:HGNC:2299"
                     /db_xref="MIM:114852"
                     /translation="MLALLVLVTVALASAHHGGEHFEGEKVFRVNVEDENHINIIREL
                     ASTTQIDFWKPDSVTQIKPHSTVDFRVKAEDTVTVENVLKQNELQYKVLISNLRNVVE
                     AQFDSRVRATGHSYEKYNKWETIEAWTQQVATENPALISRSVIGTTFEGRAIYLLKVG
                     KAGQNKPAIFMDCGFHAREWISPAFCQWFVREAVRTYGREIQVTELLDKLDFYVLPVL
                     NIDGYIYTWTKSRFWRKTRSTHTGSSCIGTDPNRNFDAGWCEIGASRNPCDETYCGPA
                     AESEKETKALADFIRNKLSSIKAYLTIHSYSQMMIYPYSYAYKLGENNAELNALAKAT
                     VKELASLHGTKYTYGPGATTIYPAAGGSDDWAYDQGIRYSFTFELRDTGRYGFLLPES
                     QIRATCEETFLAIKYVASYVLEHLY"
     exon            85..160
                     /gene="CPB1"
                     /gene_synonym="CPB; PASP; PCPB"
                     /inference="alignment:Splign:2.1.0"
     exon            161..285
                     /gene="CPB1"
                     /gene_synonym="CPB; PASP; PCPB"
                     /inference="alignment:Splign:2.1.0"
     exon            286..385
                     /gene="CPB1"
                     /gene_synonym="CPB; PASP; PCPB"
                     /inference="alignment:Splign:2.1.0"
     exon            386..487
                     /gene="CPB1"
                     /gene_synonym="CPB; PASP; PCPB"
                     /inference="alignment:Splign:2.1.0"
     exon            488..589
                     /gene="CPB1"
                     /gene_synonym="CPB; PASP; PCPB"
                     /inference="alignment:Splign:2.1.0"
     exon            590..700
                     /gene="CPB1"
                     /gene_synonym="CPB; PASP; PCPB"
                     /inference="alignment:Splign:2.1.0"
     exon            701..791
                     /gene="CPB1"
                     /gene_synonym="CPB; PASP; PCPB"
                     /inference="alignment:Splign:2.1.0"
     exon            792..994
                     /gene="CPB1"
                     /gene_synonym="CPB; PASP; PCPB"
                     /inference="alignment:Splign:2.1.0"
     exon            995..1079
                     /gene="CPB1"
                     /gene_synonym="CPB; PASP; PCPB"
                     /inference="alignment:Splign:2.1.0"
     exon            1080..1452
                     /gene="CPB1"
                     /gene_synonym="CPB; PASP; PCPB"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1424..1429
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CPB1"
                     /gene_synonym="CPB; PASP; PCPB"
                     /note="hexamer: AATAAA"
     polyA_site      1452
                     /gene="CPB1"
                     /gene_synonym="CPB; PASP; PCPB"
                     /note="major polyA site"
ORIGIN      
        1 ctggtcagac acaatgttgg cactcttggt tctggtgact gtggccctgg catctgctca
       61 tcatggtggt gagcactttg aaggcgagaa ggtgttccgt gttaacgttg aagatgaaaa
      121 tcacattaac ataatccgcg agttggccag cacgacccag attgacttct ggaagccaga
      181 ttctgtcaca caaatcaaac ctcacagtac agttgacttc cgtgttaaag cagaagatac
      241 tgtcactgtg gagaatgttc taaagcagaa tgaactacaa tacaaggtac tgataagcaa
      301 cctgagaaat gtggtggagg ctcagtttga tagccgggtt cgtgcaacag gacacagtta
      361 tgagaagtac aacaagtggg aaacgataga ggcttggact caacaagtcg ccactgagaa
      421 tccagccctc atctctcgca gtgttatcgg aaccacattt gagggacgcg ctatttacct
      481 cctgaaggtt ggcaaagctg gacaaaataa gcctgccatt ttcatggact gtggtttcca
      541 tgccagagag tggatttctc ctgcattctg ccagtggttt gtaagagagg ctgttcgtac
      601 ctatggacgt gagatccaag tgacagagct tctcgacaag ttagactttt atgtcctgcc
      661 tgtgctcaat attgatggct acatctacac ctggaccaag agccgatttt ggagaaagac
      721 tcgctccacc catactggat ctagctgcat tggcacagac cccaacagaa attttgatgc
      781 tggttggtgt gaaattggag cctctcgaaa cccctgtgat gaaacttact gtggacctgc
      841 cgcagagtct gaaaaggaga ccaaggccct ggctgatttc atccgcaaca aactctcttc
      901 catcaaggca tatctgacaa tccactcgta ctcccaaatg atgatctacc cttactcata
      961 tgcttacaaa ctcggtgaga acaatgctga gttgaatgcc ctggctaaag ctactgtgaa
     1021 agaacttgcc tcactgcacg gcaccaagta cacatatggc ccgggagcta caacaatcta
     1081 tcctgctgct gggggctctg acgactgggc ttatgaccaa ggaatcagat attccttcac
     1141 ctttgaactt cgagatacag gcagatatgg ctttctcctt ccagaatccc agatccgggc
     1201 tacctgcgag gagaccttcc tggcaatcaa gtatgttgcc agctacgtcc tggaacacct
     1261 gtactagttg agaaagctga tggccttgtt tcaaaattct catttttcat ttcttttctt
     1321 tcttgaattc ttattttggt ttgcctggat gttttgcaga tcccaatctt tcttttaagc
     1381 ttctgggtct attaaactag gtagatcttt tcgtattgat cataataaaa gtgaatcatt
     1441 actattggaa aa
//
