LOCUS       NM_016352               2790 bp    mRNA    linear   PRI 01-OCT-2024
DEFINITION  Homo sapiens carboxypeptidase A4 (CPA4), transcript variant 1,
            mRNA.
ACCESSION   NM_016352
VERSION     NM_016352.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Choi,Y.S., Jeon,M.J., Doolittle,W.K.L., Song,D.E., Kim,K., Kim,W.B.
            and Kim,W.G.
  TITLE     Macrophage-Induced Carboxypeptidase A4 Promotes the Progression of
            Anaplastic Thyroid Cancer
  JOURNAL   Thyroid 34 (9), 1150-1162 (2024)
   PUBMED   38666696
  REMARK    GeneRIF: Macrophage-Induced Carboxypeptidase A4 Promotes the
            Progression of Anaplastic Thyroid Cancer.
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Zhang,Z., Liu,W., Huang,T., Li,J., Hu,H., Xu,X. and Fan,Z.
  TITLE     CircCPA4 induces ASCT2 expression to promote tumor property of
            non-small cell lung cancer cells in a miR-145-5p-dependent manner
  JOURNAL   Thorac Cancer 15 (10), 764-777 (2024)
   PUBMED   38400818
  REMARK    GeneRIF: CircCPA4 induces ASCT2 expression to promote tumor
            property of non-small cell lung cancer cells in a
            miR-145-5p-dependent manner.
REFERENCE   3  (bases 1 to 2790)
  AUTHORS   Xu,W., Zhong,Z., Gu,L., Xiao,Y., Chen,B. and Hu,W.
  TITLE     circCPA4 induces malignant behaviors of prostate cancer via
            miR-491-5p/SHOC2 feedback loop
  JOURNAL   Clinics (Sao Paulo) 79, 100314 (2024)
   PUBMED   38219533
  REMARK    GeneRIF: circCPA4 induces malignant behaviors of prostate cancer
            via miR-491-5p/SHOC2 feedback loop.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2790)
  AUTHORS   Wang,Y., Xie,Y., Niu,Y., Song,P., Liu,Y., Burnett,J., Yang,Z.,
            Sun,D., Ran,Y., Li,Y. and Sun,L.
  TITLE     Carboxypeptidase A4 negatively correlates with p53 expression and
            regulates the stemness of breast cancer cells
  JOURNAL   Int J Med Sci 18 (8), 1753-1759 (2021)
   PUBMED   33746592
  REMARK    GeneRIF: Carboxypeptidase A4 negatively correlates with p53
            expression and regulates the stemness of breast cancer cells.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2790)
  AUTHORS   Gao,W., Guo,N., Zhao,S., Chen,Z., Zhang,W., Yan,F., Liao,H. and
            Chi,K.
  TITLE     Carboxypeptidase A4 promotes cardiomyocyte hypertrophy through
            activating PI3K-AKT-mTOR signaling
  JOURNAL   Biosci Rep 40 (5) (2020)
   PUBMED   32347291
  REMARK    GeneRIF: Carboxypeptidase A4 promotes cardiomyocyte hypertrophy
            through activating PI3K-AKT-mTOR signaling.
REFERENCE   6  (bases 1 to 2790)
  AUTHORS   Garcia-Castellanos,R., Bonet-Figueredo,R., Pallares,I., Ventura,S.,
            Aviles,F.X., Vendrell,J. and Gomis-Rutha,F.X.
  TITLE     Detailed molecular comparison between the inhibition mode of
            A/B-type carboxypeptidases in the zymogen state and by the
            endogenous inhibitor latexin
  JOURNAL   Cell Mol Life Sci 62 (17), 1996-2014 (2005)
   PUBMED   16091843
  REMARK    GeneRIF: The three-dimensional structure of procarboxypeptidase-A4
            (hPCPA4) has been solved and shows the features of related
            metallocarboxypeptidase zymogens, with a preformed
            alpha/beta/-hydrolase active-enzyme moiety (hCPA4) and an
            inhibiting pro-domain (PD).
REFERENCE   7  (bases 1 to 2790)
  AUTHORS   Bentley,L., Nakabayashi,K., Monk,D., Beechey,C., Peters,J.,
            Birjandi,Z., Khayat,F.E., Patel,M., Preece,M.A., Stanier,P.,
            Scherer,S.W. and Moore,G.E.
  TITLE     The imprinted region on human chromosome 7q32 extends to the
            carboxypeptidase A gene cluster: an imprinted candidate for
            Silver-Russell syndrome
  JOURNAL   J Med Genet 40 (4), 249-256 (2003)
   PUBMED   12676894
  REMARK    Erratum:[J Med Genet. 2004 Aug;41(8):640]
REFERENCE   8  (bases 1 to 2790)
  AUTHORS   Kayashima,T., Yamasaki,K., Yamada,T., Sakai,H., Miwa,N., Ohta,T.,
            Yoshiura,K., Matsumoto,N., Nakane,Y., Kanetake,H., Ishino,F.,
            Niikawa,N. and Kishino,T.
  TITLE     The novel imprinted carboxypeptidase A4 gene (CPA4) in the 7q32
            imprinting domain
  JOURNAL   Hum Genet 112 (3), 220-226 (2003)
   PUBMED   12552318
  REMARK    GeneRIF: CPA4 gene is imprinted, with preferential expression from
            the maternal allele in many fetal tissues, but not in fetal brain.
REFERENCE   9  (bases 1 to 2790)
  AUTHORS   Hayashida,S., Yamasaki,K., Asada,Y., Soeda,E., Niikawa,N. and
            Kishino,T.
  TITLE     Construction of a physical and transcript map flanking the
            imprinted MEST/PEG1 region at 7q32
  JOURNAL   Genomics 66 (2), 221-225 (2000)
   PUBMED   10860668
REFERENCE   10 (bases 1 to 2790)
  AUTHORS   Huang,H., Reed,C.P., Zhang,J.S., Shridhar,V., Wang,L. and
            Smith,D.I.
  TITLE     Carboxypeptidase A3 (CPA3): a novel gene highly induced by histone
            deacetylase inhibitors during differentiation of prostate
            epithelial cancer cells
  JOURNAL   Cancer Res 59 (12), 2981-2988 (1999)
   PUBMED   10383164
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC024085.5.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Feb 27, 2020 this sequence version replaced NM_016352.3.
            
            Summary: This gene is a member of the carboxypeptidase A/B
            subfamily, and it is located in a cluster with three other family
            members on chromosome 7. Carboxypeptidases are zinc-containing
            exopeptidases that catalyze the release of carboxy-terminal amino
            acids, and are synthesized as zymogens that are activated by
            proteolytic cleavage. This gene could be involved in the histone
            hyperacetylation pathway. It is imprinted and may be a strong
            candidate gene for prostate cancer aggressiveness. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY358699.1, BC052289.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142853, SAMEA2145544
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            imprinted gene         :: PMID: 12552318
            MANE Ensembl match     :: ENST00000222482.10/ ENSP00000222482.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-88                AC024085.5         110286-110373
            89-170              AC024085.5         115871-115952
            171-305             AC024085.5         116395-116529
            306-404             AC024085.5         117941-118039
            405-506             AC024085.5         121603-121704
            507-611             AC024085.5         122941-123045
            612-722             AC024085.5         123912-124022
            723-813             AC024085.5         125432-125522
            814-1013            AC024085.5         127912-128111
            1014-1098           AC024085.5         129163-129247
            1099-2790           AC024085.5         139614-141305
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q32.2"
     gene            1..2790
                     /gene="CPA4"
                     /gene_synonym="CPA3"
                     /note="carboxypeptidase A4"
                     /db_xref="GeneID:51200"
                     /db_xref="HGNC:HGNC:15740"
                     /db_xref="MIM:607635"
     exon            1..88
                     /gene="CPA4"
                     /gene_synonym="CPA3"
                     /inference="alignment:Splign:2.1.0"
     CDS             21..1286
                     /gene="CPA4"
                     /gene_synonym="CPA3"
                     /EC_number="3.4.17.15"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; carboxypeptidase A3"
                     /codon_start=1
                     /product="carboxypeptidase A4 isoform 1 preproprotein"
                     /protein_id="NP_057436.2"
                     /db_xref="CCDS:CCDS5818.1"
                     /db_xref="GeneID:51200"
                     /db_xref="HGNC:HGNC:15740"
                     /db_xref="MIM:607635"
                     /translation="MRWILFIGALIGSSICGQEKFFGDQVLRINVRNGDEISKLSQLV
                     NSNNLKLNFWKSPSSFNRPVDVLVPSVSLQAFKSFLRSQGLEYAVTIEDLQALLDNED
                     DEMQHNEGQERSSNNFNYGAYHSLEAIYHEMDNIAADFPDLARRVKIGHSFENRPMYV
                     LKFSTGKGVRRPAVWLNAGIHSREWISQATAIWTARKIVSDYQRDPAITSILEKMDIF
                     LLPVANPDGYVYTQTQNRLWRKTRSRNPGSSCIGADPNRNWNASFAGKGASDNPCSEV
                     YHGPHANSEVEVKSVVDFIQKHGNFKGFIDLHSYSQLLMYPYGYSVKKAPDAEELDKV
                     ARLAAKALASVSGTEYQVGPTCTTVYPASGSSIDWAYDNGIKFAFTFELRDTGTYGFL
                     LPANQIIPTAEETWLGLKTIMEHVRDNLY"
     exon            89..170
                     /gene="CPA4"
                     /gene_synonym="CPA3"
                     /inference="alignment:Splign:2.1.0"
     exon            171..305
                     /gene="CPA4"
                     /gene_synonym="CPA3"
                     /inference="alignment:Splign:2.1.0"
     exon            306..404
                     /gene="CPA4"
                     /gene_synonym="CPA3"
                     /inference="alignment:Splign:2.1.0"
     exon            405..506
                     /gene="CPA4"
                     /gene_synonym="CPA3"
                     /inference="alignment:Splign:2.1.0"
     exon            507..611
                     /gene="CPA4"
                     /gene_synonym="CPA3"
                     /inference="alignment:Splign:2.1.0"
     exon            612..722
                     /gene="CPA4"
                     /gene_synonym="CPA3"
                     /inference="alignment:Splign:2.1.0"
     exon            723..813
                     /gene="CPA4"
                     /gene_synonym="CPA3"
                     /inference="alignment:Splign:2.1.0"
     exon            814..1013
                     /gene="CPA4"
                     /gene_synonym="CPA3"
                     /inference="alignment:Splign:2.1.0"
     exon            1014..1098
                     /gene="CPA4"
                     /gene_synonym="CPA3"
                     /inference="alignment:Splign:2.1.0"
     exon            1099..2790
                     /gene="CPA4"
                     /gene_synonym="CPA3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2767..2772
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CPA4"
                     /gene_synonym="CPA3"
                     /note="hexamer: AATAAA"
     polyA_site      2790
                     /gene="CPA4"
                     /gene_synonym="CPA3"
                     /note="major polyA site"
ORIGIN      
        1 atgactgact ccccggggac atgaggtgga tactgttcat tggggccctt attgggtcca
       61 gcatctgtgg ccaagaaaaa ttttttgggg accaagtttt gaggattaat gtcagaaatg
      121 gagacgagat cagcaaattg agtcaactag tgaattcaaa caacttgaag ctcaatttct
      181 ggaaatctcc ctcctccttc aatcggcctg tggatgtcct ggtcccatct gtcagtctgc
      241 aggcatttaa atccttcctg agatcccagg gcttagagta cgcagtgaca attgaggacc
      301 tgcaggccct tttagacaat gaagatgatg aaatgcaaca caatgaaggg caagaacgga
      361 gcagtaataa cttcaactac ggggcttacc attccctgga agctatttac cacgagatgg
      421 acaacattgc cgcagacttt cctgacctgg cgaggagggt gaagattgga cattcgtttg
      481 aaaaccggcc gatgtatgta ctgaagttca gcactgggaa aggcgtgagg cggccggccg
      541 tttggctgaa tgcaggcatc cattcccgag agtggatctc ccaggccact gcaatctgga
      601 cggcaaggaa gattgtatct gattaccaga gggatccagc tatcacctcc atcttggaga
      661 aaatggatat tttcttgttg cctgtggcca atcctgatgg atatgtgtat actcaaactc
      721 aaaaccgatt atggaggaag acgcggtccc gaaatcctgg aagctcctgc attggtgctg
      781 acccaaatag aaactggaac gctagttttg caggaaaggg agccagcgac aacccttgct
      841 ccgaagtgta ccatggaccc cacgccaatt cggaagtgga ggtgaaatca gtggtagatt
      901 tcatccaaaa acatgggaat ttcaagggct tcatcgacct gcacagctac tcgcagctgc
      961 tgatgtatcc atatgggtac tcagtcaaaa aggccccaga tgccgaggaa ctcgacaagg
     1021 tggcgaggct tgcggccaaa gctctggctt ctgtgtcggg cactgagtac caagtgggtc
     1081 ccacctgcac cactgtctat ccagctagcg ggagcagcat cgactgggca tatgacaacg
     1141 gcatcaaatt tgcattcaca tttgagttga gagataccgg gacctatggc ttcctcctgc
     1201 cagctaacca gatcatcccc actgcagagg agacgtggct ggggctgaag accatcatgg
     1261 agcatgtgcg ggacaacctc tactaggcga tggctctgct ctgtctacat ttatttgtac
     1321 ccacacgtgc acgcactgag gccattgtta aaggagctct ttcctacctg tgtgagtcag
     1381 agccctctgg gtttgtggag cacacaggcc tgcccctctc cagccagctc cctggagtcg
     1441 tgtgtcctgg cagtgtccct gcaagaactg gttctgccag cctgctcaat tttggtcctg
     1501 ctgtttttga tgagcctttt gtctgtttct ccttccaccc tgctggctgg gcggctgcac
     1561 tcagcatcac cccttcctgg gtggcatgtc tctctctacc tcatttttag aaccaaagaa
     1621 catctgagat gattctctac cctcatccac atctagccaa gccagtgacc ttgctctggt
     1681 ggcactgtgg gagacaccac ttgtctttag gtgggtctca aagatgatgt agaatttcct
     1741 ttaatttctc gcagtcttcc tggaaaatat tttcctttga gcagcaaatc ttgtagggat
     1801 atcagtgaag gtctctccct ccctcctctc ctgttttttt ttttttgaga cagagttttg
     1861 ctcttgttgc ccaggctgga gtgtgatggc tcgatcttgg ctcaccacaa cctctgcctc
     1921 ctgggttcaa gcaattctcc tgcctcagcc tcttgagtag cttggtttat aggcgcatgc
     1981 caccatgcct ggctaatttt gtgtttttag tagagacagg gtttctccat gttggtcagg
     2041 ctggtctcaa actcccaacc tcaggtgatc tgccctcctt ggcctcccag agtgctggga
     2101 ttacaggtgt gagccactgt gccgggcccg tcccctcctt ttttaggcct gaatacaaag
     2161 tagaagatca ctttccttca ctgtgctgag aatttctaga tactacagtt cttactcctc
     2221 tcttcccttt gttattcagt gtgaccagga tggcgggagg ggatctgtgt cactgtaggt
     2281 actgtgccca ggaaggctgg gtgaagtgac catctaaatt gcaggatggt gaaattatcc
     2341 ccatctgtcc taatgggctt acctcctctt tgccttttga actcacttca aagatctagg
     2401 cctcatctta caggtcctaa atcactcatc tggcctggat aatctcactg ccctggcaca
     2461 ttcccatttg tgctgtggtg tatcctgtgt ttccttgtcc tggtttgtgt gtgtgtgtgt
     2521 gtgtgtgtgt gtgtgtgtgt gtgtgtttgt gtgtgtgtgt ctgtctattt tgtatcctgg
     2581 accacaagtt cctaagtaga gcaagaattc atcaaccagc tgcctcttgt ttcatttcac
     2641 ctcagcacgt accatctgtc cttttgttgt tgttttgttt ttgttttttt gcttttacca
     2701 aacatgtctg taaatcttaa cctcctgcct aggatttgta cagcatctgg tgtgtgctta
     2761 taagccaata aatattcaat gtgagtttca
//
