LOCUS       NM_001868               1384 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens carboxypeptidase A1 (CPA1), mRNA.
ACCESSION   NM_001868
VERSION     NM_001868.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Sandor,M., Thiel,F.G., Schmid,M., Demcsak,A., Morales Granda,N.C.,
            Nemeth,B.C., Vajda,S., Hoerning,A. and Sahin-Toth,M.
  TITLE     Novel p.G250A Mutation Associated with Chronic Pancreatitis
            Highlights Misfolding-Prone Region in Carboxypeptidase A1 (CPA1)
  JOURNAL   Int J Mol Sci 23 (24), 15463 (2022)
   PUBMED   36555104
  REMARK    GeneRIF: Novel p.G250A Mutation Associated with Chronic
            Pancreatitis Highlights Misfolding-Prone Region in Carboxypeptidase
            A1 (CPA1).
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Kawamoto,M., Kohi,S., Abe,T., Dbouk,M., Macgregor-Das,A., Koi,C.,
            Song,K.B., Borges,M., Sugimine,R., Laheru,D., Hruban,R.H.,
            Roberts,N., Klein,A.P. and Goggins,M.
  TITLE     Endoplasmic stress-inducing variants in CPB1 and CPA1 and risk of
            pancreatic cancer: A case-control study and meta-analysis
  JOURNAL   Int J Cancer 150 (7), 1123-1133 (2022)
   PUBMED   34817877
  REMARK    GeneRIF: Endoplasmic stress-inducing variants in CPB1 and CPA1 and
            risk of pancreatic cancer: A case-control study and meta-analysis.
REFERENCE   3  (bases 1 to 1384)
  AUTHORS   Uhlig,R., Contreras,H., Weidemann,S., Gorbokon,N., Menz,A.,
            Buscheck,F., Luebke,A.M., Kluth,M., Hube-Magg,C., Hinsch,A.,
            Hoflmayer,D., Fraune,C., Moller,K., Bernreuther,C., Lebok,P.,
            Sauter,G., Wilczak,W., Izbicki,J., Perez,D., Schrader,J.,
            Steurer,S., Burandt,E., Krech,R., Dum,D., Krech,T., Marx,A.,
            Simon,R., Minner,S., Jacobsen,F. and Clauditz,T.S.
  TITLE     Carboxypeptidase A1 (CPA1) Immunohistochemistry Is Highly Sensitive
            and Specific for Acinar Cell Carcinoma (ACC) of the Pancreas
  JOURNAL   Am J Surg Pathol 46 (1), 97-104 (2022)
   PUBMED   34889867
  REMARK    GeneRIF: Carboxypeptidase A1 (CPA1) Immunohistochemistry Is Highly
            Sensitive and Specific for Acinar Cell Carcinoma (ACC) of the
            Pancreas.
REFERENCE   4  (bases 1 to 1384)
  AUTHORS   Masamune,A.
  TITLE     Genetics of pancreatitis: the 2014 update
  JOURNAL   Tohoku J Exp Med 232 (2), 69-77 (2014)
   PUBMED   24522117
  REMARK    Review article
REFERENCE   5  (bases 1 to 1384)
  AUTHORS   Shelton,C., LaRusch,J. and Whitcomb,D.C.
  TITLE     Pancreatitis Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   24624459
REFERENCE   6  (bases 1 to 1384)
  AUTHORS   Catasus,L., Villegas,V., Pascual,R., Aviles,F.X.,
            Wicker-Planquart,C. and Puigserver,A.
  TITLE     cDNA cloning and sequence analysis of human pancreatic
            procarboxypeptidase A1
  JOURNAL   Biochem J 287 (Pt 1) (Pt 1), 299-303 (1992)
   PUBMED   1417781
REFERENCE   7  (bases 1 to 1384)
  AUTHORS   Stewart,E.A., Craik,C.S., Hake,L. and Bowcock,A.M.
  TITLE     Human carboxypeptidase A identifies a BglII RFLP and maps to
            7q31-qter
  JOURNAL   Am J Hum Genet 46 (4), 795-800 (1990)
   PUBMED   1969228
REFERENCE   8  (bases 1 to 1384)
  AUTHORS   Moulard,M., Michon,T., Kerfelec,B. and Chapus,C.
  TITLE     Further studies on the human pancreatic binary complexes involving
            procarboxypeptidase A
  JOURNAL   FEBS Lett 261 (1), 179-183 (1990)
   PUBMED   2307232
REFERENCE   9  (bases 1 to 1384)
  AUTHORS   Pascual,R., Burgos,F.J., Salva,M., Soriano,F., Mendez,E. and
            Aviles,F.X.
  TITLE     Purification and properties of five different forms of human
            procarboxypeptidases
  JOURNAL   Eur J Biochem 179 (3), 609-616 (1989)
   PUBMED   2920728
REFERENCE   10 (bases 1 to 1384)
  AUTHORS   Honey,N.K., Sakaguchi,A.Y., Lalley,P.A., Quinto,C., Rutter,W.J. and
            Naylor,S.L.
  TITLE     Assignment of the gene for carboxypeptidase A to human chromosome
            7q22----qter and to mouse chromosome 6
  JOURNAL   Hum Genet 72 (1), 27-31 (1986)
   PUBMED   3455919
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK291493.1 and X67318.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001868.3.
            
            Summary: This gene encodes a member of the carboxypeptidase A
            family of zinc metalloproteases. This enzyme is produced in the
            pancreas and preferentially cleaves C-terminal branched-chain and
            aromatic amino acids from dietary proteins. This gene and several
            family members are present in a gene cluster on chromosome 7.
            Mutations in this gene may be linked to chronic pancreatitis, while
            elevated protein levels may be associated with pancreatic cancer.
            [provided by RefSeq, Jan 2015].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK291493.1, BT007313.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000011292.8/ ENSP00000011292.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1383              AK291493.1         1-1383
            1384-1384           X67318.1           1364-1364
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q32.2"
     gene            1..1384
                     /gene="CPA1"
                     /gene_synonym="CPA"
                     /note="carboxypeptidase A1"
                     /db_xref="GeneID:1357"
                     /db_xref="HGNC:HGNC:2296"
                     /db_xref="MIM:114850"
     exon            1..92
                     /gene="CPA1"
                     /gene_synonym="CPA"
                     /inference="alignment:Splign:2.1.0"
     CDS             28..1287
                     /gene="CPA1"
                     /gene_synonym="CPA"
                     /EC_number="3.4.17.1"
                     /note="pancreatic carboxypeptidase A; carboxypeptidase A1
                     (pancreatic)"
                     /codon_start=1
                     /product="carboxypeptidase A1 preproprotein"
                     /protein_id="NP_001859.1"
                     /db_xref="CCDS:CCDS5820.1"
                     /db_xref="GeneID:1357"
                     /db_xref="HGNC:HGNC:2296"
                     /db_xref="MIM:114850"
                     /translation="MRGLLVLSVLLGAVFGKEDFVGHQVLRISVADEAQVQKVKELED
                     LEHLQLDFWRGPAHPGSPIDVRVPFPSIQAVKIFLESHGISYETMIEDVQSLLDEEQE
                     QMFAFRSRARSTDTFNYATYHTLEEIYDFLDLLVAENPHLVSKIQIGNTYEGRPIYVL
                     KFSTGGSKRPAIWIDTGIHSREWVTQASGVWFAKKITQDYGQDAAFTAILDTLDIFLE
                     IVTNPDGFAFTHSTNRMWRKTRSHTAGSLCIGVDPNRNWDAGFGLSGASSNPCSETYH
                     GKFANSEVEVKSIVDFVKDHGNIKAFISIHSYSQLLMYPYGYKTEPVPDQDELDQLSK
                     AAVTALASLYGTKFNYGSIIKAIYQASGSTIDWTYSQGIKYSFTFELRDTGRYGFLLP
                     ASQIIPTAKETWLALLTIMEHTLNHPY"
     exon            93..174
                     /gene="CPA1"
                     /gene_synonym="CPA"
                     /inference="alignment:Splign:2.1.0"
     exon            175..408
                     /gene="CPA1"
                     /gene_synonym="CPA"
                     /inference="alignment:Splign:2.1.0"
     exon            409..510
                     /gene="CPA1"
                     /gene_synonym="CPA"
                     /inference="alignment:Splign:2.1.0"
     exon            511..612
                     /gene="CPA1"
                     /gene_synonym="CPA"
                     /inference="alignment:Splign:2.1.0"
     exon            613..723
                     /gene="CPA1"
                     /gene_synonym="CPA"
                     /inference="alignment:Splign:2.1.0"
     exon            724..814
                     /gene="CPA1"
                     /gene_synonym="CPA"
                     /inference="alignment:Splign:2.1.0"
     exon            815..1014
                     /gene="CPA1"
                     /gene_synonym="CPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1015..1099
                     /gene="CPA1"
                     /gene_synonym="CPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1100..1384
                     /gene="CPA1"
                     /gene_synonym="CPA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1342..1347
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CPA1"
                     /gene_synonym="CPA"
                     /note="hexamer: AATAAA"
     polyA_site      1384
                     /gene="CPA1"
                     /gene_synonym="CPA"
                     /note="major polyA site"
ORIGIN      
        1 agtctgacct tccctcccgg cagcagcatg cgggggttgc tggtgttgag tgtcctgttg
       61 ggggctgtct ttggcaagga ggactttgtg gggcatcagg tgctccgaat ctctgtagcc
      121 gatgaggccc aggtacagaa ggtgaaggag ctggaggacc tggagcacct gcagctggac
      181 ttctggcggg ggcctgccca ccctggctcc cccatcgacg tccgagtgcc cttccccagc
      241 atccaggcgg tcaagatctt tctggagtcc cacggcatca gctatgagac catgatcgag
      301 gacgtgcagt cgctgctgga cgaggagcag gagcagatgt tcgccttccg gtcccgggcg
      361 cgctccaccg acacttttaa ctacgccacc taccacaccc tggaggagat ctatgacttc
      421 ctggacctgc tggtggcgga gaacccgcac cttgtcagca agatccagat tggcaacacc
      481 tatgaagggc gtcccattta cgtgctgaag ttcagcacgg ggggcagtaa gcgtccagcc
      541 atctggatcg acacgggcat ccattcccgg gagtgggtca cccaggccag tggggtctgg
      601 tttgcaaaga agatcactca agactacggg caggatgcag ctttcaccgc cattctcgac
      661 accttggaca tcttcctgga gatcgtcacc aaccctgatg gctttgcctt cacgcacagc
      721 acgaatcgca tgtggcgcaa gactcggtcc cacacagcag gctccctctg tattggcgtg
      781 gaccccaaca ggaactggga cgctggcttt gggttgtccg gagccagcag taacccctgc
      841 tcggagactt accacggcaa gtttgccaat tccgaagtgg aggtcaagtc cattgtagac
      901 tttgtgaagg accatgggaa catcaaggcc ttcatctcca tccacagcta ctcccagctc
      961 ctcatgtatc cctatggcta caaaacagaa ccagtccctg accaggatga gctggatcag
     1021 ctttccaagg ctgctgtgac agccctggcc tctctctacg ggaccaagtt caactatggc
     1081 agcatcatca aggcaattta tcaagccagt ggaagcacta ttgactggac ctacagccag
     1141 ggcatcaagt actccttcac cttcgagctc cgggacactg ggcgctatgg cttcctgctg
     1201 ccagcctccc agatcatccc cacagccaag gagacgtggc tggcgcttct gaccatcatg
     1261 gagcacaccc tgaatcaccc ctactgagct gaccctttga cacccttctt gtcctcctct
     1321 ctggccccat ccaggcaacc aaataaagtt tgagtgtacc aggaacagaa tcctggggct
     1381 tgca
//
