LOCUS       NM_021149               1842 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens coactosin like F-actin binding protein 1 (COTL1),
            mRNA.
ACCESSION   NM_021149
VERSION     NM_021149.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Xia,L., Xiao,X., Liu,W.L., Song,Y., Liu,T.J.J., Li,Y.J.,
            Zacksenhaus,E., Hao,X.J. and Ben-David,Y.
  TITLE     Coactosin-like protein CLP/Cotl1 suppresses breast cancer growth
            through activation of IL-24/PERP and inhibition of non-canonical
            TGFbeta signaling
  JOURNAL   Oncogene 37 (3), 323-331 (2018)
   PUBMED   28925397
  REMARK    GeneRIF: Study uncovers a novel tumor-suppressor role for CLP/Cotl1
            and identify the downstream effectors interleukin 24 (IL-24)/PERP
            and IL-24/MAPK/ERK/TGF-beta.
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Guo,S., Yang,P., Jiang,X., Li,X., Wang,Y., Zhang,X., Sun,B.,
            Zhang,Y. and Jia,Y.
  TITLE     Genetic and epigenetic silencing of mircoRNA-506-3p enhances COTL1
            oncogene expression to foster non-small lung cancer progression
  JOURNAL   Oncotarget 8 (1), 644-657 (2017)
   PUBMED   27893417
  REMARK    GeneRIF: our combined results elucidated genetic and epigenetic
            silencing of miR-506-3p enhances COTL1 oncogene expression to
            foster NSCLC progression.
REFERENCE   3  (bases 1 to 1842)
  AUTHORS   Basavarajappa,D., Wan,M., Lukic,A., Steinhilber,D., Samuelsson,B.
            and Radmark,O.
  TITLE     Roles of coactosin-like protein (CLP) and 5-lipoxygenase-activating
            protein (FLAP) in cellular leukotriene biosynthesis
  JOURNAL   Proc Natl Acad Sci U S A 111 (31), 11371-11376 (2014)
   PUBMED   25034252
  REMARK    GeneRIF: The roles of CLP and FLAP in in cellular leukotriene
            biosynthesis, were studied.
REFERENCE   4  (bases 1 to 1842)
  AUTHORS   Kim,J., Shapiro,M.J., Bamidele,A.O., Gurel,P., Thapa,P.,
            Higgs,H.N., Hedin,K.E., Shapiro,V.S. and Billadeau,D.D.
  TITLE     Coactosin-like 1 antagonizes cofilin to promote lamellipodial
            protrusion at the immune synapse
  JOURNAL   PLoS One 9 (1), e85090 (2014)
   PUBMED   24454796
  REMARK    GeneRIF: COTL1 regulates lamellipodia dynamics in part by
            protecting F-actin from cofilin-mediated disassembly.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1842)
  AUTHORS   Sun,W., Guo,C., Meng,X., Yu,Y., Jin,Y., Tong,D., Geng,J., Huang,Q.,
            Qi,J., Liu,A., Guan,R., Xu,L., Sun,D., Ji,W., Liu,P., Liu,F.,
            Sun,H., Ji,G., Fu,S. and Bai,J.
  TITLE     Differential expression of PAI-RBP1, C1orf142, and COTL1 in
            non-small cell lung cancer cell lines with different tumor
            metastatic potential
  JOURNAL   J Investig Med 60 (4), 689-694 (2012)
   PUBMED   22373659
  REMARK    GeneRIF: PAI-RBP1 and C1orf142 expression levels are higher in 95D
            (high metastatic potential) than in 95C (low metastatic potential)
            non-small lung cancer cells, whereas COTL1 expression level is
            lower in 95D when compared to 95C cells.
REFERENCE   6  (bases 1 to 1842)
  AUTHORS   Provost,P., Doucet,J., Stock,A., Gerisch,G., Samuelsson,B. and
            Radmark,O.
  TITLE     Coactosin-like protein, a human F-actin-binding protein: critical
            role of lysine-75
  JOURNAL   Biochem J 359 (Pt 2), 255-263 (2001)
   PUBMED   11583571
REFERENCE   7  (bases 1 to 1842)
  AUTHORS   Provost,P., Doucet,J., Hammarberg,T., Gerisch,G., Samuelsson,B. and
            Radmark,O.
  TITLE     5-Lipoxygenase interacts with coactosin-like protein
  JOURNAL   J Biol Chem 276 (19), 16520-16527 (2001)
   PUBMED   11297527
REFERENCE   8  (bases 1 to 1842)
  AUTHORS   Provost,P., Samuelsson,B. and Radmark,O.
  TITLE     Interaction of 5-lipoxygenase with cellular proteins
  JOURNAL   Proc Natl Acad Sci U S A 96 (5), 1881-1885 (1999)
   PUBMED   10051563
REFERENCE   9  (bases 1 to 1842)
  AUTHORS   Chen,K.S., Manian,P., Koeuth,T., Potocki,L., Zhao,Q.,
            Chinault,A.C., Lee,C.C. and Lupski,J.R.
  TITLE     Homologous recombination of a flanking repeat gene cluster is a
            mechanism for a common contiguous gene deletion syndrome
  JOURNAL   Nat Genet 17 (2), 154-163 (1997)
   PUBMED   9326934
REFERENCE   10 (bases 1 to 1842)
  AUTHORS   Wu,C., Friedlander,P., Lamoureux,C., Zannis-Hadjopoulos,M. and
            Price,G.B.
  TITLE     cDNA clones contain autonomous replication activity
  JOURNAL   Biochim Biophys Acta 1174 (3), 241-257 (1993)
   PUBMED   7690594
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC092145.2 and AC022165.9.
            
            On Aug 31, 2019 this sequence version replaced NM_021149.4.
            
            Summary: This gene encodes one of the numerous actin-binding
            proteins which regulate the actin cytoskeleton. This protein binds
            F-actin, and also interacts with 5-lipoxygenase, which is the first
            committed enzyme in leukotriene biosynthesis. Although this gene
            has been reported to map to chromosome 17 in the Smith-Magenis
            syndrome region, the best alignments for this gene are to
            chromosome 16. The Smith-Magenis syndrome region is the site of two
            related pseudogenes. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC010884.1, L54057.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA2142670
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000262428.5/ ENSP00000262428.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-241               AC092145.2         82603-82843         c
            242-324             AC092145.2         82266-82348         c
            325-482             AC092145.2         54870-55027         c
            483-1842            AC022165.9         209692-211051       c
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q24.1"
     gene            1..1842
                     /gene="COTL1"
                     /gene_synonym="CLP"
                     /note="coactosin like F-actin binding protein 1"
                     /db_xref="GeneID:23406"
                     /db_xref="HGNC:HGNC:18304"
                     /db_xref="MIM:606748"
     exon            1..241
                     /gene="COTL1"
                     /gene_synonym="CLP"
                     /inference="alignment:Splign:2.1.0"
     CDS             165..593
                     /gene="COTL1"
                     /gene_synonym="CLP"
                     /note="coactosin-like 1; epididymis secretory sperm
                     binding protein"
                     /codon_start=1
                     /product="coactosin-like protein"
                     /protein_id="NP_066972.1"
                     /db_xref="CCDS:CCDS10947.1"
                     /db_xref="GeneID:23406"
                     /db_xref="HGNC:HGNC:18304"
                     /db_xref="MIM:606748"
                     /translation="MATKIDKEACRAAYNLVRDDGSAVIWVTFKYDGSTIVPGEQGAE
                     YQHFIQQCTDDVRLFAFVRFTTGDAMSKRSKFALITWIGENVSGLQRAKTGTDKTLVK
                     EVVQNFAKEFVISDRKELEEDFIKSELKKAGGANYDAQTE"
     exon            242..324
                     /gene="COTL1"
                     /gene_synonym="CLP"
                     /inference="alignment:Splign:2.1.0"
     exon            325..482
                     /gene="COTL1"
                     /gene_synonym="CLP"
                     /inference="alignment:Splign:2.1.0"
     exon            483..1842
                     /gene="COTL1"
                     /gene_synonym="CLP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1814..1819
                     /regulatory_class="polyA_signal_sequence"
                     /gene="COTL1"
                     /gene_synonym="CLP"
                     /note="hexamer: AATAAA"
     polyA_site      1842
                     /gene="COTL1"
                     /gene_synonym="CLP"
                     /note="major polyA site"
ORIGIN      
        1 agtggaagcc gccgccgcgc tcgcagcttg caggcgccgc gtagccgtcg ccaccgccgc
       61 cagcccgtgc gccctcggcg cgtacccgcc gcgctcccat ccccgccgcc ggccaggggc
      121 gcgctcggcc gccccggaca gtgtcccgct gcggctccgc ggcgatggcc accaagatcg
      181 acaaagaggc ttgccgggcg gcgtacaacc tggtgcgcga cgacggctcg gccgtcatct
      241 gggtgacttt taaatatgac ggctccacca tcgtccccgg cgagcaggga gcggagtacc
      301 agcacttcat ccagcagtgc acagatgacg tccggttgtt tgccttcgtg cgcttcacca
      361 ccggggatgc catgagcaag aggtccaagt ttgccctcat cacgtggatc ggtgagaacg
      421 tcagcgggct gcagcgcgcc aaaaccggga cggacaagac cctggtgaag gaggtcgtac
      481 agaatttcgc taaggagttt gtgatcagtg atcggaagga gctggaggaa gatttcatca
      541 agagcgagct gaagaaggcg gggggagcca attacgacgc ccagacggag taaccccagc
      601 ccccgccaca ccaccccttg ccaaagtcat ctgcctgctc cccgggggag aggaccgccg
      661 gcctcagcta ctagcccacc agcccaccag ggagaaaaga agccatgaga ggcagcgccc
      721 gccaccctgt gtccacagcc cccaccttcc cgcttccctt agaaccctgc cgtgtcctat
      781 ctcatgacgc tcatggaacc tctttctttg atcttctttt tcttttctcc ccctcttttt
      841 tgttctaaag aaaagtcatt ttgatgcaag gtcctgcctg ccatcagatc cgaggtgcct
      901 cctgcagtga ccccttttcc tggcatttct cttccacgcg acgaggtctg cctagtgaga
      961 tctgcatgac ctcacgttgc tttccagagc ccgggcctat tttgccatct cagttttcct
     1021 ggaccctgct tcctgtgtac cactgagggg cagctgggcc aggagctgtg cccggtgcct
     1081 gcagccttca taagcacaca cgtccattcc ctactaaggc ccagacctcc tggtatctgc
     1141 cccgggctcc ctcatcccac ctccatccgg agttgcctaa gatgcatgtc cagcataggc
     1201 aggattgctc ggtggtgaga aggttaggtc cggctcagac tgaataagaa gagataaaat
     1261 ttgccttaaa acttacctgg cagtggcttt gctgcacggt ctgaaaccac ctgttcccac
     1321 cctcttgacc gaaatttcct tgtgacacag agaagggcaa aggtctgagc ccagagttga
     1381 cggagggagt atttcagggt tcacttcagg ggctcccaaa gcgacaagat cgttagggag
     1441 agaggcccag ggtggggact gggaatttaa ggagagctgg gaacggatcc cttaggttca
     1501 ggaagcttct gtgtaagctg cgaggatggc ttgggccgaa gggttgctct gcccgccgcg
     1561 ctagctgtga gctgagcaaa gccctgggct cacagcaccc caaaagcctg tggcttcagt
     1621 cctgcgtctg caccacacat tcaaaaggat cgttttgttt tgtttttaaa gaaaggtgag
     1681 attggcttgg ttcttcatga gcacatttga tatagctctt tttctgtttt tccttgctca
     1741 tttcgttttg gggaagaaat ctgtactgta ttgggattgt aaagaacatc tctgcactca
     1801 gacagtttac agaaataaat gttttttttg tttttcagaa aa
//
