LOCUS       NM_004371               5318 bp    mRNA    linear   PRI 31-OCT-2024
DEFINITION  Homo sapiens COPI coat complex subunit alpha (COPA), transcript
            variant 2, mRNA.
ACCESSION   NM_004371
VERSION     NM_004371.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5318)
  AUTHORS   Delafontaine,S., Iannuzzo,A., Bigley,T.M., Mylemans,B., Rana,R.,
            Baatsen,P., Poli,M.C., Rymen,D., Jansen,K., Mekahli,D.,
            Casteels,I., Cassiman,C., Demaerel,P., Lepelley,A., Fremond,M.L.,
            Schrijvers,R., Bossuyt,X., Vints,K., Huybrechts,W., Tacine,R.,
            Willekens,K., Corveleyn,A., Boeckx,B., Baggio,M., Ehlers,L.,
            Munck,S., Lambrechts,D., Voet,A., Moens,L., Bucciol,G.,
            Cooper,M.A., Davis,C.M., Delon,J. and Meyts,I.
  TITLE     Heterozygous mutations in the C-terminal domain of COPA underlie a
            complex autoin fl ammatory syndrome
  JOURNAL   J Clin Invest 134 (4), e163604 (2024)
   PUBMED   38175705
  REMARK    GeneRIF: Heterozygous mutations in the C-terminal domain of COPA
            underlie a complex autoin fl ammatory syndrome.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5318)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 5318)
  AUTHORS   Dey,D., Singh,S., Khan,S., Martin,M., Schnicker,N.J., Gakhar,L.,
            Pierce,B.G. and Hasan,S.S.
  TITLE     An extended motif in the SARS-CoV-2 spike modulates binding and
            release of host coatomer in retrograde trafficking
  JOURNAL   Commun Biol 5 (1), 115 (2022)
   PUBMED   35136165
  REMARK    GeneRIF: An extended motif in the SARS-CoV-2 spike modulates
            binding and release of host coatomer in retrograde trafficking.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5318)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 5318)
  AUTHORS   Lepelley,A., Martin-Niclos,M.J., Le Bihan,M., Marsh,J.A.,
            Uggenti,C., Rice,G.I., Bondet,V., Duffy,D., Hertzog,J.,
            Rehwinkel,J., Amselem,S., Boulisfane-El Khalifi,S., Brennan,M.,
            Carter,E., Chatenoud,L., Chhun,S., Coulomb l'Hermine,A., Depp,M.,
            Legendre,M., Mackenzie,K.J., Marey,J., McDougall,C., McKenzie,K.J.,
            Molina,T.J., Neven,B., Seabra,L., Thumerelle,C., Wislez,M.,
            Nathan,N., Manel,N., Crow,Y.J. and Fremond,M.L.
  TITLE     Mutations in COPA lead to abnormal trafficking of STING to the
            Golgi and interferon signaling
  JOURNAL   J Exp Med 217 (11) (2020)
   PUBMED   32725128
  REMARK    GeneRIF: Mutations in COPA lead to abnormal trafficking of STING to
            the Golgi and interferon signaling.
REFERENCE   6  (bases 1 to 5318)
  AUTHORS   Schekman,R. and Orci,L.
  TITLE     Coat proteins and vesicle budding
  JOURNAL   Science 271 (5255), 1526-1533 (1996)
   PUBMED   8599108
  REMARK    Review article
REFERENCE   7  (bases 1 to 5318)
  AUTHORS   Lowe,M. and Kreis,T.E.
  TITLE     In vitro assembly and disassembly of coatomer
  JOURNAL   J Biol Chem 270 (52), 31364-31371 (1995)
   PUBMED   8537409
REFERENCE   8  (bases 1 to 5318)
  AUTHORS   Cukierman,E., Huber,I., Rotman,M. and Cassel,D.
  TITLE     The ARF1 GTPase-activating protein: zinc finger motif and Golgi
            complex localization
  JOURNAL   Science 270 (5244), 1999-2002 (1995)
   PUBMED   8533093
REFERENCE   9  (bases 1 to 5318)
  AUTHORS   Orcl,L., Palmer,D.J., Amherdt,M. and Rothman,J.E.
  TITLE     Coated vesicle assembly in the Golgi requires only coatomer and ARF
            proteins from the cytosol
  JOURNAL   Nature 364 (6439), 732-734 (1993)
   PUBMED   8355790
REFERENCE   10 (bases 1 to 5318)
  AUTHORS   Feurle,G.E., Hamscher,G., Kusiek,R., Meyer,H.E. and Metzger,J.W.
  TITLE     Identification of xenin, a xenopsin-related peptide, in the human
            gastric mucosa and its effect on exocrine pancreatic secretion
  JOURNAL   J Biol Chem 267 (31), 22305-22309 (1992)
   PUBMED   1429581
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC308331.1, U24105.1 and
            AL513282.10.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Dec 14, 2018 this sequence version replaced NM_004371.3.
            
            Summary: In eukaryotic cells, protein transport between the
            endoplasmic reticulum and Golgi compartments is mediated in part by
            non-clathrin-coated vesicular coat proteins (COPs). Seven coat
            proteins have been identified, and they represent subunits of a
            complex known as coatomer. The subunits are designated alpha-COP,
            beta-COP, beta-prime-COP, gamma-COP, delta-COP, epsilon-COP, and
            zeta-COP. The alpha-COP, encoded by COPA, shares high sequence
            similarity with RET1P, the alpha subunit of the coatomer complex in
            yeast. Also, the N-terminal 25 amino acids of alpha-COP encode the
            bioactive peptide, xenin, which stimulates exocrine pancreatic
            secretion and may act as a gastrointestinal hormone. Alternative
            splicing results in multiple splice forms encoding distinct
            isoforms. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 3' coding region, compared to variant 1,
            resulting in a shorter protein (isoform 2).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.172274.1,
                                           SRR1803616.271524.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000241704.8/ ENSP00000241704.7
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-220               DC308331.1         315-534
            221-442             U24105.1           607-828
            443-443             AL513282.10        69371-69371         c
            444-2186            U24105.1           830-2572
            2187-2187           AL513282.10        33361-33361         c
            2188-4306           U24105.1           2574-4692
            4307-5318           AL513282.10        24339-25350         c
FEATURES             Location/Qualifiers
     source          1..5318
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q23.2"
     gene            1..5318
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /note="COPI coat complex subunit alpha"
                     /db_xref="GeneID:1314"
                     /db_xref="HGNC:HGNC:2230"
                     /db_xref="MIM:601924"
     exon            1..120
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     CDS             81..3755
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /note="isoform 2 is encoded by transcript variant 2;
                     xenin; alpha coat protein; proxenin; coatomer protein
                     complex subunit alpha"
                     /codon_start=1
                     /product="coatomer subunit alpha isoform 2"
                     /protein_id="NP_004362.2"
                     /db_xref="CCDS:CCDS1202.1"
                     /db_xref="GeneID:1314"
                     /db_xref="HGNC:HGNC:2230"
                     /db_xref="MIM:601924"
                     /translation="MLTKFETKSARVKGLSFHPKRPWILTSLHNGVIQLWDYRMCTLI
                     DKFDEHDGPVRGIDFHKQQPLFVSGGDDYKIKVWNYKLRRCLFTLLGHLDYIRTTFFH
                     HEYPWILSASDDQTIRVWNWQSRTCVCVLTGHNHYVMCAQFHPTEDLVVSASLDQTVR
                     VWDISGLRKKNLSPGAVESDVRGITGVDLFGTTDAVVKHVLEGHDRGVNWAAFHPTMP
                     LIVSGADDRQVKIWRMNESKAWEVDTCRGHYNNVSCAVFHPRQELILSNSEDKSIRVW
                     DMSKRTGVQTFRRDHDRFWVLAAHPNLNLFAAGHDGGMIVFKLERERPAYAVHGNMLH
                     YVKDRFLRQLDFNSSKDVAVMQLRSGSKFPVFNMSYNPAENAVLLCTRASNLENSTYD
                     LYTIPKDADSQNPDAPEGKRSSGLTAVWVARNRFAVLDRMHSLLIKNLKNEITKKVQV
                     PNCDEIFYAGTGNLLLRDADSITLFDVQQKRTLASVKISKVKYVIWSADMSHVALLAK
                     HAIVICNRKLDALCNIHENIRVKSGAWDESGVFIYTTSNHIKYAVTTGDHGIIRTLDL
                     PIYVTRVKGNNVYCLDRECRPRVLTIDPTEFKFKLALINRKYDEVLHMVRNAKLVGQS
                     IIAYLQKKGYPEVALHFVKDEKTRFSLALECGNIEIALEAAKALDDKNCWEKLGEVAL
                     LQGNHQIVEMCYQRTKNFDKLSFLYLITGNLEKLRKMMKIAEIRKDMSGHYQNALYLG
                     DVSERVRILKNCGQKSLAYLTAATHGLDEEAESLKETFDPEKETIPDIDPNAKLLQPP
                     APIMPLDTNWPLLTVSKGFFEGTIASKGKGGALAADIDIDTVGTEGWGEDAELQLDED
                     GFVEATEGLGDDALGKGQEEGGGWDVEEDLELPPELDISPGAAGGAEDGFFVPPTKGT
                     SPTQIWCNNSQLPVDHILAGSFETAMRLLHDQVGVIQFGPYKQLFLQTYARGRTTYQA
                     LPCLPSMYGYPNRNWKDAGLKNGVPAVGLKLNDLIQRLQLCYQLTTVGKFEEAVEKFR
                     SILLSVPLLVVDNKQEIAEAQQLITICREYIVGLSVETERKKLPKETLEQQKRICEMA
                     AYFTHSNLQPVHMILVLRTALNLFFKLKNFKTAATFARRLLELGPKPEVAQQTRKILS
                     ACEKNPTDAYQLNYDMHNPFDICAASYRPIYRGKPVEKCPLSGACYSPEFKGQICRVT
                     TVTEIGKDVIGLRISPLQFR"
     exon            121..234
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     exon            235..308
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     exon            309..389
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     exon            390..466
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     exon            467..576
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     exon            577..686
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     exon            687..786
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     exon            787..922
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     exon            923..1005
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     exon            1006..1156
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     exon            1157..1223
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     exon            1224..1299
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     exon            1300..1382
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     exon            1383..1522
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     exon            1523..1608
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     exon            1609..1747
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     exon            1748..1910
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     exon            1911..2057
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     exon            2058..2247
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     exon            2248..2343
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     exon            2344..2432
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     exon            2433..2556
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     exon            2557..2646
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     exon            2647..2756
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     exon            2757..2834
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     exon            2835..2903
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     exon            2904..3040
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     exon            3041..3227
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     exon            3228..3338
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     exon            3339..3500
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     exon            3501..3695
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     exon            3696..5318
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4614..4619
                     /regulatory_class="polyA_signal_sequence"
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /note="hexamer: AATAAA"
     polyA_site      4639
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /note="major polyA site"
     regulatory      5250..5255
                     /regulatory_class="polyA_signal_sequence"
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /note="hexamer: AATAAG"
     polyA_site      5274
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
     regulatory      5293..5298
                     /regulatory_class="polyA_signal_sequence"
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
                     /note="hexamer: AATAAA"
     polyA_site      5318
                     /gene="COPA"
                     /gene_synonym="AIAISD; AILJK; alpha-COP; HEP-COP"
ORIGIN      
        1 gtcgctgacg tggaggcgtc cgaagggcag cagggtgtgt cggggctcgg attaagacat
       61 cggagtcgga gacctgagag atgttaacca aattcgagac caagagcgcg cgggtcaaag
      121 ggctcagctt tcaccccaaa agaccttgga tcctgactag tttacataat ggggtcatcc
      181 agttatggga ctatcggatg tgcactctca ttgacaagtt tgatgaacat gatggtccag
      241 tgcgaggcat tgacttccat aagcagcagc cactgttcgt ctctggagga gatgactata
      301 agattaaggt ttggaattac aagcttcggc gctgtctttt cacattgctt gggcacttag
      361 attatattcg caccacgttt tttcatcatg aatatccctg gattctgagt gcctccgatg
      421 atcagaccat ccgagtgtgg aactggcaat ctagaacctg tgtttgtgtg ttaacagggc
      481 acaaccatta tgtgatgtgt gctcagttcc accccacaga agacttggta gtatcagcca
      541 gcctggacca gactgtgcgc gtttgggata tttctggtct gaggaaaaaa aacctgtccc
      601 ctggtgcggt ggaatcggat gtgagaggaa taactggggt tgatctattt ggaactacag
      661 atgcagtggt gaagcatgta ctagagggtc acgatcgtgg agtaaactgg gctgccttcc
      721 accccactat gccccttatt gtatctgggg cagatgatcg tcaagtgaag atctggcgca
      781 tgaatgaatc aaaggcatgg gaggttgata cctgccgggg ccattacaac aatgtatctt
      841 gtgccgtctt ccaccctcgc caagagttga tcctcagcaa ttctgaggac aagagtattc
      901 gagtctggga tatgtctaag cggactgggg ttcagacttt ccgcagagac catgatcgtt
      961 tctgggtcct agctgctcac cctaacctta acctctttgc agcaggccat gatggtggta
     1021 tgattgtgtt taagctggaa cgggaacggc cagcctatgc tgttcatggc aatatgctac
     1081 actatgtcaa ggaccgattc ttacgacagc tggatttcaa cagctccaaa gatgtagctg
     1141 tgatgcagtt gcggagtggt tccaagtttc cagtattcaa tatgtcatac aatccagcag
     1201 aaaatgcagt cctgctttgt acaagagcta gcaatctaga gaatagtacc tatgacctgt
     1261 acaccatccc taaagatgct gactcccaga atcctgatgc gcctgaaggg aaacgatcct
     1321 caggcctgac agccgtttgg gtcgctcgaa atcggtttgc tgtcctagat cggatgcatt
     1381 cgcttctgat caagaatctg aagaatgaga tcaccaaaaa ggtacaggtg cccaactgtg
     1441 atgagatctt ctatgctggc acaggcaatc tcctgcttcg agatgcggac tctatcacac
     1501 tctttgacgt acagcagaag cggactctgg catctgtgaa gatttctaaa gtgaaatacg
     1561 ttatctggtc agcagacatg tcacatgtag cactactagc caaacacgcc attgtgatct
     1621 gtaaccgcaa actggatgct ttatgtaaca ttcatgagaa cattcgtgtc aagagtgggg
     1681 cctgggatga gagtggggta tttatctata ccacaagcaa ccacatcaaa tatgctgtca
     1741 ccactgggga ccacgggatc attcgaactc tggatttacc catctatgtc acacgggtga
     1801 agggcaacaa tgtatactgc ctagacaggg agtgtcgtcc ccgggtactc accattgatc
     1861 ccactgagtt caaattcaag ctggccctga tcaacagaaa atatgatgag gtactgcaca
     1921 tggtgaggaa tgccaaacta gttggccagt ctattattgc ttatctccag aagaagggct
     1981 atcctgaagt ggcactgcat tttgtcaagg atgagaaaac tcgctttagt ctggcactgg
     2041 agtgtggaaa cattgagatt gctctggaag cagccaaagc actggatgac aagaactgct
     2101 gggaaaagct gggagaagtg gccctgctgc aggggaacca ccagattgtg gaaatgtgct
     2161 atcagcgtac caaaaacttt gacaaacttt ccttcctgta tcttatcact ggcaacttag
     2221 aaaaacttcg caagatgatg aagattgctg agatcagaaa ggacatgagt ggccactatc
     2281 agaatgccct atacctgggt gatgtgtcag agcgtgtgcg gatcctgaag aactgtggac
     2341 agaagtccct ggcctatctc acagctgcta cccatggctt agatgaagaa gctgagagcc
     2401 taaaggagac atttgaccca gagaaggaga caatcccaga cattgaccct aatgccaagc
     2461 tgctccagcc acctgcacct atcatgccat tggataccaa ttggccttta ttgactgtat
     2521 ccaaaggatt ttttgaaggc accattgcca gcaaagggaa gggaggagca ctggctgctg
     2581 acattgacat tgacactgtt ggtacagagg gctggggaga ggatgcagag ctgcagttgg
     2641 atgaagatgg gtttgtggag gctacagaag gtttggggga tgatgctctt ggcaagggac
     2701 aggaagaagg aggtggctgg gatgtagaag aagatctgga gctccctcct gagctggata
     2761 tatcccctgg ggcagctggt ggggctgaag atggtttctt tgtgccccca accaagggaa
     2821 caagtccaac tcagatctgg tgtaataact ctcagcttcc agttgatcac atcctggcag
     2881 gctctttcga aacagccatg cggctccttc atgaccaagt aggggtaatc cagtttggcc
     2941 cctacaagca actgttccta cagacatacg cccgaggccg cacaacctat caggctctgc
     3001 cctgcctacc ctccatgtat ggctatccta atcgcaactg gaaggatgca gggctgaaga
     3061 atggtgtacc agctgtgggc ctgaagctta atgacctcat ccaacggttg cagctgtgct
     3121 accagctcac cacagttggc aaatttgagg aggctgtgga aaaattccgt tccatccttc
     3181 tcagtgtgcc acttcttgtt gtggacaata aacaagagat tgcagaggcc cagcagctca
     3241 tcaccatttg ccgtgagtac attgtgggtt tgtccgtgga gacagaaagg aagaagctgc
     3301 ccaaagagac tctagaacag cagaagcgca tctgtgagat ggcagcctat ttcacccact
     3361 caaacctgca gcctgtgcac atgatcctgg tgctgcgtac agccctcaat ctgttcttca
     3421 agctcaagaa cttcaagaca gctgccacct ttgctcggcg cctactagaa ctcgggccca
     3481 agcctgaggt ggcccaacag acccgaaaaa tcctgtctgc ctgtgagaag aatcccacag
     3541 atgcctacca gctcaattat gacatgcaca acccctttga catttgtgct gcatcatatc
     3601 ggcccatcta ccgtggaaag ccagtagaaa agtgtccact cagtggggcc tgctattccc
     3661 ctgagttcaa aggtcaaatc tgcagggtca ccacagtgac agagattggc aaagatgtga
     3721 ttggtttaag gatcagtcct ctgcagtttc gctaaggccc cctttgtgtg catgggtcag
     3781 tcaccatatg ttccccccag agaatgtgtc tatatcctcc ttctaacagc accttccccc
     3841 tgcagctact cttcagatct ggctctctgt accctaaaac ctagtatctt tttctcttct
     3901 atggaaaatc cgaaggtcta aacttgactt ttttgaggtc ttctcaactt gactacagtt
     3961 gtgctcataa ttgtccttgc ctttccagct taattatttt aaggaacaaa tgaaaactct
     4021 gggctgggtg gagtggctca tacctgtaat cccagcactt tgggaggcta cggtgggcag
     4081 atcatctgag gccaggagtt cgagacctgc ctggccaaca tggcaacacc ccgtctctaa
     4141 taaaaatata aaaattagcc tggcatggta gcatgcgcct atagtcccag ctgctcagga
     4201 ggctgaggca tgagaatcgc ttgaacctag gaggtggagg ttgcattcaa ctgagatcat
     4261 accacttcat tccagcctgg gtgacagagc aagactctgt ctcaaaaaaa aaaaaaagga
     4321 aaactctgtg atggacattt gtttagtaaa tcccttcagt atttatccct cctttcccca
     4381 cagcagcttt ctttcctgtc aactagaaag gagcaggatg taataaatac attttggtgt
     4441 gactaggcca caccaactct taatcatctc ccattttcct tagacattta aatttcaagg
     4501 caggtaccct ctgtgtactc agaaatttga agaagttatt tggttttcca aaatgcacac
     4561 tgcgggttat tgatttgttc tttacaacta ttgttctcat atttctcaca ctaaataaat
     4621 ctctatgaga gcttcttgac ttggccattt atttcttgga cactctcatg ttcttgttca
     4681 cccatgcagg caccccacca aagtacatat cttccttcca gtaataattt ttaattacaa
     4741 aataaacatc cactattgga aaaaaaaaaa aaaagctagc cgggcatggt ggtgggtgcc
     4801 tgtaatccca gctactctgg aggctgaggc agaggattgc ttgaacccgg gaggcggagg
     4861 ttgcagtaag ctgagatcgc gccaccgcac tccagcctgg gcgacagagt gagactccat
     4921 ctcaaaaaaa aagaaagaaa aaaagaagca catgtttttc atagggtata tatgaggacc
     4981 taaactgctg tgaaaatgat agaaagcaag tagctccctt attctgtttt tgattgcagc
     5041 cttttatctt ttgctaatta tagcaatatt tattgagcac ctgccatgtg actgtcactg
     5101 ttctagatat tttacatgta atatacagat aaaagaatag tactttatat atattacaat
     5161 gatacaatga ttacattaac aatacaatat tttgcttgtc atatgctaag aataattggg
     5221 tagagtgaca ttactgtgcc ttcgattaaa ataagtactt ttttgcgtgt taaattcatg
     5281 ttttcaataa ataataaatg catatagttg aaaaatca
//
