LOCUS       NM_024656               3647 bp    mRNA    linear   PRI 07-SEP-2021
DEFINITION  Homo sapiens collagen beta(1-O)galactosyltransferase 1 (COLGALT1),
            mRNA.
ACCESSION   NM_024656
VERSION     NM_024656.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Mori K, Suzuki T, Miura K, Dohmae N and Simizu S.
  TITLE     Involvement of LH3 and GLT25D1 for
            glucosyl-galactosyl-hydroxylation on non-collagen-like domain of
            FGL1
  JOURNAL   Biochem Biophys Res Commun 560, 93-98 (2021)
   PUBMED   33984770
  REMARK    GeneRIF: Involvement of LH3 and GLT25D1 for
            glucosyl-galactosyl-hydroxylation on non-collagen-like domain of
            FGL1.
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Hennet T.
  TITLE     Collagen glycosylation
  JOURNAL   Curr Opin Struct Biol 56, 131-138 (2019)
   PUBMED   30822656
  REMARK    Review article
REFERENCE   3  (bases 1 to 3647)
  AUTHORS   Webster JA, Yang Z, Kim YH, Loo D, Mosa RM, Li H and Chen C.
  TITLE     Collagen beta (1-O) galactosyltransferase 1 (GLT25D1) is required
            for the secretion of high molecular weight adiponectin and affects
            lipid accumulation
  JOURNAL   Biosci Rep 37 (3) (2017)
   PUBMED   28428430
  REMARK    GeneRIF: GLT25D1 regulates HMW adiponectin secretion.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3647)
  AUTHORS   Baumann S and Hennet T.
  TITLE     Collagen Accumulation in Osteosarcoma Cells lacking GLT25D1
            Collagen Galactosyltransferase
  JOURNAL   J Biol Chem 291 (35), 18514-18524 (2016)
   PUBMED   27402836
  REMARK    GeneRIF: Loss of GLT25D1 led to increased expression and
            intracellular accumulation of collagen type I, whereas loss of
            GLT25D2 had no effect on collagen secretion. Inactivation of the
            GLT25D1 gene resulted in a compensatory induction of GLT25D2
            expression.
REFERENCE   5  (bases 1 to 3647)
  AUTHORS   Perrin-Tricaud C, Rutschmann C and Hennet T.
  TITLE     Identification of domains and amino acids essential to the collagen
            galactosyltransferase activity of GLT25D1
  JOURNAL   PLoS One 6 (12), e29390 (2011)
   PUBMED   22216269
  REMARK    GeneRIF: introduced substitution Q471R-V472M-N473Q-P474V in the
            CEECAM1-C-terminal construct, which is found in most animal GLT25D1
            and GLT25D2 isoforms but not in CEECAM1. This substitution was
            shown to partially restore collagen galactosyltransferase activity
REFERENCE   6  (bases 1 to 3647)
  AUTHORS   Liefhebber JM, Punt S, Spaan WJ and van Leeuwen HC.
  TITLE     The human collagen beta(1-O)galactosyltransferase, GLT25D1, is a
            soluble endoplasmic reticulum localized protein
  JOURNAL   BMC Cell Biol 11, 33 (2010)
   PUBMED   20470363
  REMARK    GeneRIF: Data indicate that galactosylation of collagenous proteins
            by the soluble GLT25D1 occurs in the early secretory pathway
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 3647)
  AUTHORS   Schegg B, Hulsmeier AJ, Rutschmann C, Maag C and Hennet T.
  TITLE     Core glycosylation of collagen is initiated by two
            beta(1-O)galactosyltransferases
  JOURNAL   Mol Cell Biol 29 (4), 943-952 (2009)
   PUBMED   19075007
  REMARK    GeneRIF: Two collagen beta(1-O)galactosyltransferases corresponding
            to the GLT25D1 and GLT25D2 proteins were identified.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK074941.1, AC010618.9,
            AK075541.1 and BU628916.1.
            
            On Nov 22, 2018 this sequence version replaced NM_024656.3.
            
            Summary: The protein encoded by this gene is one of two enzymes
            that transfers galactose moieties to hydroxylysine residues of
            collagen and mannose binding lectin. This gene is constitutively
            expressed and encodes a soluble protein that localizes to the
            endoplasmic reticulum. [provided by RefSeq, Dec 2015].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC108308.1, AK075541.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000252599.9/ ENSP00000252599.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-178               AK074941.1         60-237
            179-179             AC010618.9         66067-66067
            180-2004            AK075541.1         127-1951
            2005-3361           AC010618.9         91755-93111
            3362-3647           BU628916.1         23-308              c
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.11"
     gene            1..3647
                     /gene="COLGALT1"
                     /gene_synonym="BSVD3; ColGalT 1; GLT25D1"
                     /note="collagen beta(1-O)galactosyltransferase 1"
                     /db_xref="GeneID:79709"
                     /db_xref="HGNC:HGNC:26182"
                     /db_xref="MIM:617531"
     exon            1..325
                     /gene="COLGALT1"
                     /gene_synonym="BSVD3; ColGalT 1; GLT25D1"
                     /inference="alignment:Splign:2.1.0"
     CDS             66..1934
                     /gene="COLGALT1"
                     /gene_synonym="BSVD3; ColGalT 1; GLT25D1"
                     /EC_number="2.4.1.50"
                     /note="procollagen galactosyltransferase 1; hydroxylysine
                     galactosyltransferase 1; glycosyltransferase 25 family
                     member 1; glycosyltransferase 25 domain containing 1"
                     /codon_start=1
                     /product="procollagen galactosyltransferase 1 precursor"
                     /protein_id="NP_078932.2"
                     /db_xref="CCDS:CCDS12363.1"
                     /db_xref="GeneID:79709"
                     /db_xref="HGNC:HGNC:26182"
                     /db_xref="MIM:617531"
                     /translation="MAAAPRAGRRRGQPLLALLLLLLAPLPPGAPPGADAYFPEERWS
                     PESPLQAPRVLIALLARNAAHALPTTLGALERLRHPRERTALWVATDHNMDNTSTVLR
                     EWLVAVKSLYHSVEWRPAEEPRSYPDEEGPKHWSDSRYEHVMKLRQAALKSARDMWAD
                     YILFVDADNLILNPDTLSLLIAENKTVVAPMLDSRAAYSNFWCGMTSQGYYKRTPAYI
                     PIRKRDRRGCFAVPMVHSTFLIDLRKAASRNLAFYPPHPDYTWSFDDIIVFAFSCKQA
                     EVQMYVCNKEEYGFLPVPLRAHSTLQDEAESFMHVQLEVMVKHPPAEPSRFISAPTKT
                     PDKMGFDEVFMINLRRRQDRRERMLRALQAQEIECRLVEAVDGKAMNTSQVEALGIQM
                     LPGYRDPYHGRPLTKGELGCFLSHYNIWKEVVDRGLQKSLVFEDDLRFEIFFKRRLMN
                     LMRDVEREGLDWDLIYVGRKRMQVEHPEKAVPRVRNLVEADYSYWTLAYVISLQGARK
                     LLAAEPLSKMLPVDEFLPVMFDKHPVSEYKAHFSLRNLHAFSVEPLLIYPTHYTGDDG
                     YVSDTETSVVWNNEHVKTDWDRAKSQKMREQQALSREAKNSDVLQSPLDSAARDEL"
     exon            326..436
                     /gene="COLGALT1"
                     /gene_synonym="BSVD3; ColGalT 1; GLT25D1"
                     /inference="alignment:Splign:2.1.0"
     exon            437..554
                     /gene="COLGALT1"
                     /gene_synonym="BSVD3; ColGalT 1; GLT25D1"
                     /inference="alignment:Splign:2.1.0"
     exon            555..689
                     /gene="COLGALT1"
                     /gene_synonym="BSVD3; ColGalT 1; GLT25D1"
                     /inference="alignment:Splign:2.1.0"
     exon            690..894
                     /gene="COLGALT1"
                     /gene_synonym="BSVD3; ColGalT 1; GLT25D1"
                     /inference="alignment:Splign:2.1.0"
     exon            895..1014
                     /gene="COLGALT1"
                     /gene_synonym="BSVD3; ColGalT 1; GLT25D1"
                     /inference="alignment:Splign:2.1.0"
     exon            1015..1091
                     /gene="COLGALT1"
                     /gene_synonym="BSVD3; ColGalT 1; GLT25D1"
                     /inference="alignment:Splign:2.1.0"
     exon            1092..1198
                     /gene="COLGALT1"
                     /gene_synonym="BSVD3; ColGalT 1; GLT25D1"
                     /inference="alignment:Splign:2.1.0"
     exon            1199..1331
                     /gene="COLGALT1"
                     /gene_synonym="BSVD3; ColGalT 1; GLT25D1"
                     /inference="alignment:Splign:2.1.0"
     exon            1332..1459
                     /gene="COLGALT1"
                     /gene_synonym="BSVD3; ColGalT 1; GLT25D1"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..1666
                     /gene="COLGALT1"
                     /gene_synonym="BSVD3; ColGalT 1; GLT25D1"
                     /inference="alignment:Splign:2.1.0"
     exon            1667..3647
                     /gene="COLGALT1"
                     /gene_synonym="BSVD3; ColGalT 1; GLT25D1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3623..3628
                     /regulatory_class="polyA_signal_sequence"
                     /gene="COLGALT1"
                     /gene_synonym="BSVD3; ColGalT 1; GLT25D1"
                     /note="hexamer: AATAAA"
     polyA_site      3647
                     /gene="COLGALT1"
                     /gene_synonym="BSVD3; ColGalT 1; GLT25D1"
                     /note="major polyA site"
ORIGIN      
        1 gaccccttta aggcgcggcc agagtcctcc cgcagaaaaa cgacttaaag gagacgcgtg
       61 gcgcgatggc ggcggcccca cgcgcgggcc ggcggcgcgg gcagccgctc ctggcgctgc
      121 tgcttctgct gctggcgcca ctgccgccgg gggccccgcc gggcgccgac gcctacttcc
      181 ccgaggagcg ctggagcccg gagtcgcccc tgcaggcgcc gcgcgtgctc atcgcgctgt
      241 tggcgcgaaa cgcggcccac gcgttgccca ccacgctggg cgcactcgag cggctgcggc
      301 acccgcggga gcgcacggcg ctatgggtgg ctacggacca caacatggat aacacgtcaa
      361 ctgtgctgcg ggagtggctg gtggccgtga agagtttgta ccattccgtg gagtggcggc
      421 cagcagagga gcccaggtcc tacccggacg aggaaggccc gaaacactgg tctgactcac
      481 gctacgagca tgtcatgaag ttgcgccagg cagccctgaa atcagctcga gacatgtggg
      541 ctgattacat cctgtttgta gatgcggaca acctgatcct caaccctgac acactgagcc
      601 tgctcatcgc tgagaacaag acggtggtcg cccccatgct ggattcccgg gctgcgtact
      661 ccaacttctg gtgtggaatg acttcccagg gctactacaa gcgcacacct gcctacatcc
      721 ctatccgcaa gcgagaccgc cggggctgct ttgcagttcc catggtgcac tcgaccttcc
      781 tgatcgacct gcggaaggcg gcgtccagga acctggcctt ctacccacct caccctgact
      841 acacctggtc ctttgacgac atcatcgtct ttgccttctc ctgcaagcag gcagaggttc
      901 agatgtatgt gtgcaacaag gaggagtacg gattcttgcc agtgccattg cgcgcccaca
      961 gcaccctcca ggatgaggcc gagagcttca tgcatgtgca gctggaggtc atggtgaagc
     1021 acccgcccgc agagccctcc cgcttcatct cggctcccac caagacaccg gacaagatgg
     1081 gcttcgacga ggtcttcatg atcaacctga ggcggcggca ggaccggcgg gagcgcatgc
     1141 tgcgggcgct gcaggcacag gagatcgagt gccggctggt ggaggccgtg gacggcaaag
     1201 ccatgaacac cagccaggtg gaggcgctgg ggatccagat gctgcctggc taccgggacc
     1261 cctaccacgg ccggcccctc accaagggtg agctgggctg cttcctgagc cactacaaca
     1321 tctggaagga ggtggtggac cgggggctgc agaaatcgct tgtgtttgag gatgacctgc
     1381 gttttgagat cttcttcaag agacgtctga tgaacctcat gcgggatgtg gagcgggagg
     1441 gcctggactg ggacctcatc tatgtgggcc ggaagcggat gcaggtggag caccccgaga
     1501 aggctgtgcc tcgcgtgagg aacctggtgg aggccgacta ttcctactgg accctggcct
     1561 acgtgatctc cctgcaaggc gcccgcaaac tgctggctgc tgagccgctc tccaagatgc
     1621 tgcctgtgga cgagttcctg cccgtcatgt tcgacaaaca cccagtgtcc gagtacaagg
     1681 cccacttctc cctccgcaac ctgcatgcct tctctgtgga gccgctgctc atctacccca
     1741 cacactacac aggagacgat ggctatgtga gtgacaccga gacctcagtc gtatggaaca
     1801 atgagcacgt caagaccgac tgggaccgcg ccaagtccca gaagatgcgg gagcagcagg
     1861 cactgagccg tgaggccaag aactcggacg tgctccagtc cccactggac agtgctgccc
     1921 gggatgaact ctgaggggta gcagccagaa agccaaagca gccatcggtg gcccaggctc
     1981 cacgtgctta ctgaggacat caggtccacc tctggacccc ttggcaggcc acagagggct
     2041 ctcgtgtggg gtggtgtcca gccagctctt gctaagcaat cacgtgcaca caggcagcat
     2101 taatggagtg cctactgcat gccagcaaca gggcttggcc ctggggaatt gggaggaacc
     2161 aagccctctt catctgttca tgtgcccagc atttattaag cacctgctgt atgcaaggtt
     2221 cccatgttac ggcagtgaat gaggcataat tgttccctcc atcagcgatt gattcagtca
     2281 tcaagcagtt actgatcaga ttaagaatca ggcactagtg atacacattc atttttaaaa
     2341 ttcattcaag gatttattga gtgcctactg tgtgttgggt gccattccag gctctgggat
     2401 tttttttttt ttttttttaa gagtagagtc tgtctctgtc acccaggctg gagtgcagtg
     2461 gtgtgacggc tcactgcagc ctgcgcctcc cagcgtccag caattcttgt ttctcggcct
     2521 cccaagtagc tgggactata ggtgcgtgcc atcacatctg gctagttttt gtatttttag
     2581 tagagacggg gtttcaccat gttggccagg ctggtctcga actcctgacc tcaagtgatc
     2641 tgcccgcctt ggccacccaa agtgctaggg ttacaggcat gagccactgt gcccagccca
     2701 ggctctggga atattgagat aaataagatg cttctatcca tttattcaag cacatattgg
     2761 tcacctactg tgtgcctggc actcatgtca caaagataag ttcctgattc ggtacactta
     2821 ctgagcacct gctgtgtgca gggagctgag ctatgggatg ggaatgggag taaacaaggt
     2881 actttttact tttttctttt tttcctcact gctagacggt gtgggaactt ctcactcatt
     2941 ggcttctttc ccacacacct gaagagcact gactgtgtgc cgggcactag tgatacaaaa
     3001 gagtgtgaca gttgttcagt ctgcattttc gatcatgggc tacatgccga gtgctggggc
     3061 acagagatga acaagatcgg ttccttcact tcttcatgcc acaagtgttt attgagcacc
     3121 tgtgtgccag gcctcacaga ctcccagttg ggttgaagaa tggttgactg agtttgattc
     3181 ttcctgtacc ctcggtcgtc tgagctgtgt gcagacaaca tccccccacc acccaagagg
     3241 gagggtagct cttccgccac caggggcaag cacaggtcct ggtggcccca cgccacatgt
     3301 tagcccccct ggagggggcg ccagttggag acgggggctg ggtgtccctg gcccactccc
     3361 ggtcccctgt gctttacctc cttgcccttg tgtctcaggt gtggtccctg cctgcttgat
     3421 gaagttgctc tgttcaagcc tttggtggga tcatgtgttt gggggctttt aggggaccca
     3481 gctgcactgg ggcactgccc gtggcctggg taggacattt cccagcaagg gctggaggag
     3541 ttgccgtgcc ttcagcctga atcgaatgtc agaaccagcc agcggtgctt caccctcttg
     3601 gggataactt gcttagtttt ttaataaatg ttcctggttg gttttca
//
