LOCUS       NM_001102608            8689 bp    mRNA    linear   PRI 16-SEP-2022
DEFINITION  Homo sapiens collagen type VI alpha 6 chain (COL6A6), mRNA.
ACCESSION   NM_001102608 XM_067585 XM_940071
VERSION     NM_001102608.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8689)
  AUTHORS   Vaclavik V, Tiab L, Sun YJ, Mahajan VB, Moulin A, Allaman-Pillet N,
            Munier FL and Schorderet DF.
  TITLE     New COL6A6 Variant Causes Autosomal Dominant Retinitis Pigmentosa
            in a Four-Generation Family
  JOURNAL   Invest Ophthalmol Vis Sci 63 (3), 23 (2022)
   PUBMED   35333290
  REMARK    GeneRIF: New COL6A6 Variant Causes Autosomal Dominant Retinitis
            Pigmentosa in a Four-Generation Family.
REFERENCE   2  (bases 1 to 8689)
  AUTHORS   Liang C, Wang P, Liu X, Yang C, Ma Y, Yong L, Zhu B, Liu X and Liu
            Z.
  TITLE     Whole-genome sequencing reveals novel genes in ossification of the
            posterior longitudinal ligament of the thoracic spine in the
            Chinese population
  JOURNAL   J Orthop Surg Res 13 (1), 324 (2018)
   PUBMED   30577800
  REMARK    GeneRIF: Whole-genome sequencing reveals possible role of
            deleterious mutation of COL6A6 in ossification of the posterior
            longitudinal ligament of the thoracic spine in the Chinese
            population.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8689)
  AUTHORS   Naba A, Pearce OMT, Del Rosario A, Ma D, Ding H, Rajeeve V,
            Cutillas PR, Balkwill FR and Hynes RO.
  TITLE     Characterization of the Extracellular Matrix of Normal and Diseased
            Tissues Using Proteomics
  JOURNAL   J Proteome Res 16 (8), 3083-3091 (2017)
   PUBMED   28675934
REFERENCE   4  (bases 1 to 8689)
  AUTHORS   Heo WI, Park KY, Jin T, Lee MK, Kim M, Choi EH, Kim HS, Bae JM,
            Moon NJ and Seo SJ.
  TITLE     Identification of novel candidate variants including COL6A6
            polymorphisms in early-onset atopic dermatitis using whole-exome
            sequencing
  JOURNAL   BMC Med Genet 18 (1), 8 (2017)
   PUBMED   28125976
  REMARK    GeneRIF: Study identified SNPs in COL6A6 associated with atopic
            dermatitis suggesting that COL6A6 variants may constitute candidate
            risk factors for early-onset atopic dermatitis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8689)
  AUTHORS   Barallobre-Barreiro J, Gupta SK, Zoccarato A, Kitazume-Taneike R,
            Fava M, Yin X, Werner T, Hirt MN, Zampetaki A, Viviano A, Chong M,
            Bern M, Kourliouros A, Domenech N, Willeit P, Shah AM, Jahangiri M,
            Schaefer L, Fischer JW, Iozzo RV, Viner R, Thum T, Heineke J,
            Kichler A, Otsu K and Mayr M.
  TITLE     Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin
            Activity in Human Atrial Fibrillation
  JOURNAL   Circulation 134 (11), 817-832 (2016)
   PUBMED   27559042
REFERENCE   6  (bases 1 to 8689)
  AUTHORS   Sabatelli P, Gualandi F, Gara SK, Grumati P, Zamparelli A, Martoni
            E, Pellegrini C, Merlini L, Ferlini A, Bonaldo P, Maraldi NM,
            Paulsson M, Squarzoni S and Wagener R.
  TITLE     Expression of collagen VI alpha5 and alpha6 chains in human muscle
            and in Duchenne muscular dystrophy-related muscle fibrosis
  JOURNAL   Matrix Biol 31 (3), 187-196 (2012)
   PUBMED   22226732
  REMARK    GeneRIF: The collagen VI alpha6 chain, but not the alpha5 chain, is
            up-regulated in dystrophic muscles.
REFERENCE   7  (bases 1 to 8689)
  AUTHORS   Groulx JF, Gagne D, Benoit YD, Martel D, Basora N and Beaulieu JF.
  TITLE     Collagen VI is a basement membrane component that regulates
            epithelial cell-fibronectin interactions
  JOURNAL   Matrix Biol 30 (3), 195-206 (2011)
   PUBMED   21406227
  REMARK    GeneRIF: collagen VI alpha 6 is an important basal lamina component
            involved in the regulation of epithelial cell behavior most notably
            as a regulator of epithelial cell-fibronectin interactions
REFERENCE   8  (bases 1 to 8689)
  AUTHORS   Sabatelli P, Gara SK, Grumati P, Urciuolo A, Gualandi F, Curci R,
            Squarzoni S, Zamparelli A, Martoni E, Merlini L, Paulsson M,
            Bonaldo P and Wagener R.
  TITLE     Expression of the collagen VI alpha5 and alpha6 chains in normal
            human skin and in skin of patients with collagen VI-related
            myopathies
  JOURNAL   J Invest Dermatol 131 (1), 99-107 (2011)
   PUBMED   20882040
  REMARK    GeneRIF: localization of alpha5, and to a lesser extent alpha6, is
            restricted to the papillary dermis, where the protein mainly
            colocalizes with collagen fibrils; both chains were found around
            blood vessels
REFERENCE   9  (bases 1 to 8689)
  AUTHORS   Fitzgerald J, Rich C, Zhou FH and Hansen U.
  TITLE     Three novel collagen VI chains, alpha4(VI), alpha5(VI), and
            alpha6(VI)
  JOURNAL   J Biol Chem 283 (29), 20170-20180 (2008)
   PUBMED   18400749
  REMARK    GeneRIF: The discovery of three additional collagen VI chains
            doubles the collagen VI family and adds a layer of complexity to
            collagen VI assembly and function in the extracellular matrix.
REFERENCE   10 (bases 1 to 8689)
  AUTHORS   Gara SK, Grumati P, Urciuolo A, Bonaldo P, Kobbe B, Koch M,
            Paulsson M and Wagener R.
  TITLE     Three novel collagen VI chains with high homology to the alpha3
            chain
  JOURNAL   J Biol Chem 283 (16), 10658-10670 (2008)
   PUBMED   18276594
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC117398.3, AC093006.4 and
            AC128683.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Feb 5, 2021 this sequence version replaced NM_001102608.2.
            
            Summary: This gene encodes a large protein that contains multiple
            von Willebrand factor domains and forms a component of the basal
            lamina of epithelial cells. This protein may regulate epithelial
            cell-fibronectin interactions. Variation in this gene may be
            implicated in skin diseases. [provided by RefSeq, May 2017].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA2142363,
                              SAMEA2142853 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            MANE Ensembl match               :: ENST00000358511.11/
                                                ENSP00000351310.6
            RefSeq Select criteria           :: based on manual assertion,
                                                conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-221               AC117398.3         4609-4829           c
            222-316             AC093006.4         111116-111210
            317-913             AC093006.4         113850-114446
            914-1534            AC093006.4         115776-116396
            1535-2095           AC093006.4         117484-118044
            2096-2653           AC093006.4         118829-119386
            2654-3229           AC093006.4         121600-122175
            3230-3799           AC093006.4         124738-125307
            3800-4143           AC093006.4         132343-132686
            4144-4222           AC093006.4         132772-132850
            4223-4377           AC093006.4         137288-137442
            4378-4470           AC093006.4         139872-139964
            4471-4524           AC093006.4         141823-141876
            4525-4596           AC093006.4         143323-143394
            4597-4623           AC093006.4         143478-143504
            4624-4668           AC093006.4         143843-143887
            4669-4722           AC093006.4         143981-144034
            4723-4785           AC093006.4         145063-145125
            4786-4851           AC093006.4         149147-149212
            4852-4905           AC093006.4         150539-150592
            4906-4941           AC093006.4         157713-157748
            4942-5004           AC093006.4         159684-159746
            5005-5067           AC093006.4         161431-161493
            5068-5130           AC093006.4         172603-172665
            5131-5193           AC093006.4         177267-177329
            5194-5244           AC093006.4         178101-178151
            5245-5280           AC093006.4         185372-185407
            5281-5343           AC093006.4         186481-186543
            5344-5406           AC093006.4         192434-192496
            5407-5442           AC093006.4         193614-193649
            5443-5479           AC093006.4         193769-193805
            5480-5491           AC093006.4         195773-195784
            5492-5985           AC093006.4         199851-200344
            5986-6082           AC093006.4         209458-209554
            6083-6754           AC093006.4         212419-213090
            6755-6848           AC128683.3         2212-2305
            6849-8689           AC128683.3         12411-14251
FEATURES             Location/Qualifiers
     source          1..8689
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q22.1"
     gene            1..8689
                     /gene="COL6A6"
                     /note="collagen type VI alpha 6 chain"
                     /db_xref="GeneID:131873"
                     /db_xref="HGNC:HGNC:27023"
                     /db_xref="MIM:616613"
     exon            1..221
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            222..316
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     CDS             253..7044
                     /gene="COL6A6"
                     /note="collagen, type VI, alpha 6"
                     /codon_start=1
                     /product="collagen alpha-6(VI) chain precursor"
                     /protein_id="NP_001096078.1"
                     /db_xref="CCDS:CCDS46911.1"
                     /db_xref="GeneID:131873"
                     /db_xref="HGNC:HGNC:27023"
                     /db_xref="MIM:616613"
                     /translation="MMLLILFLVIICSHISVNQDSGPEYADVVFLVDSSDRLGSKSFP
                     FVKMFITKMISSLPIEADKYRVALAQYSDKLHSEFHLSTFKGRSPMLNHLRKNFGFIG
                     GSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLASSESEDNVEEASKALRKDGVK
                     IISVGVQKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVIKYKEGAVDDIF
                     VEACQGPSMADVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNE
                     TKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQ
                     IAVLVTHRDSEDNVTKAAVNLRREGVTIFTLGIEGASDTQLEKIASHPAEQYVSKLKT
                     FADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQAT
                     DFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQ
                     MGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEPANRLREEHIR
                     VYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEICTEEACKEMKADIM
                     FLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQ
                     SDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKE
                     PAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGICSP
                     REECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADD
                     PEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKGVPQV
                     LIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETF
                     GGLKGIFSDVTASVCNSSKVDCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDF
                     DVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEH
                     YFRPDMGSRINTGTPQVLLVLTDGQSQDEVAQAAEALRHRGIDIYSVGIGDVDDQQLI
                     QITGTAEKKLTVHNFDELKKVNKRIVRNICTTAGESNCFVDVVVGFDVSTQEKGQTLL
                     EGQPWMETYLQDILRAISSLNGVSCEVGTETQVSVAFQVTNAMEKYSPKFEIYSENIL
                     NSLKDITVKGPSLLNANLLDSLWDTFQNKSAARGKVVLLFSDGLDDDVEKLEQKSDEL
                     RKEGLNALITVALDGPADSSDLADLPYIEFGKGFEYRTQLSIGMRELGSRLSKQLVNV
                     AERTCCCLFCKCIGGDGTMGDPGPPGKRGPPGFKGSEGYLGEEGIAGERGAPGPVGEQ
                     GTKGCYGTKGPKGNRGLNGQEGEVGENGIDGLNGEQGDNGLPGRKGEKGDEGSQGSPG
                     KRGTPGDRGAKGLRGDPGAPGVDSSIEGPTGLKGERGRQGRRGWPGPPGTPGSRRKTA
                     AHGRRGHTGPQGTAGIPGPDGLEGSLGLKGPQGPRGEAGVKGEKGGVGSKGPQGPPGP
                     GGEAGNQGRLGSQGNKGEPGDLGEKGAVGFPGPRGLQGNDGSPGYGSVGRKGAKGQEG
                     FPGESGPKGEIGDPGGPGETGLKGARGKMISAGLPGEMGSPGEPGPPGRKGVKGAKGL
                     ASFSTCELIQYVRDRSPGRHGKPECPVHPTELVFALDHSRDVTEQEFERMKEMMAFLV
                     RDIKVRENSCPVGAHIAILSYNSHARHLVRFSDAYKKSQLLREIETIPYERSSASREI
                     GRAMRFISRNVFKRTLPGAHTRKIATFFSSGQSADAHSITTAAMEFGALEIIPVVITF
                     SNVPSVRRAFAIDDTGTFQVIVVPSGADYIPALERLQRCTFCYDVCKPDASCDQARPP
                     PVQSYMDAAFLLDASRNMGSAEFEDIRAFLGALLDHFEITPEPETSVTGDRVALLSHA
                     PPDFLPNTQKSPVRAEFNLTTYRSKRLMKRHVHESVKQLNGDAFIGHALQWTLDNVFL
                     STPNLRRNKVIFVISAGETSHLDGEILKKESLRAKCQGYALFVFSLGPIWDDKELEDL
                     ASHPLDHHLVQLGRIHKPDHSYGVKFVKSFINSIRRAINKYPPINLKIKCNRLNSIDP
                     KQPPRPFRSFVPGPLKATLKEDVLQKAKFFQDKKYLSRVARSGRDDAIQNFMRSTSHT
                     FKNGRMIESAPKQHD"
     exon            317..913
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            914..1534
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            1535..2095
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            2096..2653
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            2654..3229
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            3230..3799
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            3800..4143
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            4144..4222
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            4223..4377
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            4378..4470
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            4471..4524
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            4525..4596
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            4597..4623
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            4624..4668
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            4669..4722
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            4723..4785
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            4786..4851
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            4852..4905
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            4906..4941
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            4942..5004
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            5005..5067
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            5068..5130
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            5131..5193
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            5194..5244
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            5245..5280
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            5281..5343
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            5344..5406
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            5407..5442
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            5443..5479
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            5480..5491
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            5492..5985
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            5986..6082
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            6083..6754
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            6755..6848
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     exon            6849..8689
                     /gene="COL6A6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8562..8567
                     /regulatory_class="polyA_signal_sequence"
                     /gene="COL6A6"
                     /note="hexamer: AATAAA"
     polyA_site      8585
                     /gene="COL6A6"
                     /note="major polyA site"
     regulatory      8670..8675
                     /regulatory_class="polyA_signal_sequence"
                     /gene="COL6A6"
                     /note="hexamer: AGTAAA"
     polyA_site      8689
                     /gene="COL6A6"
                     /note="major polyA site"
ORIGIN      
        1 gccacccgct tgcctgcggg acaccggagt caagaggctg cccacgccgc tgcctgtccg
       61 ttccacggtt ccgaggtctc caccgtctcc ccgcgcgccg caggcggcac caaactctgc
      121 gctccccgcg gtgcgccctg cccgcgcagt gcgcgtccag aggaaatccg ccccgggctt
      181 tcgaagtgca gggctggggg ctgcatggaa gtttccccaa gatttgaagt tgaagatttt
      241 tcaggtcata atatgatgtt gctaattttg ttcctcgtga taatttgttc ccatatttct
      301 gtgaaccaag attccggccc tgagtatgca gatgttgtgt ttttggtgga cagctctgat
      361 cgcctgggat ccaagtcctt cccatttgtg aaaatgttca tcaccaaaat gatcagcagt
      421 ctccccatag aggccgacaa ataccgtgtg gccctggccc agtacagtga taaacttcac
      481 agtgaattcc acctgagcac cttcaaaggc aggagcccca tgctgaacca cctaaggaag
      541 aactttggat tcattggcgg gtccctgcag ataggaaagg ctcttcagga ggctcacagg
      601 acttatttct ctgcacccgc aaatgggaga gacaagaaac agtttccccc aattctagtg
      661 gtcctggctt catctgagtc tgaggataat gtggaagagg catcaaaggc cctgcggaaa
      721 gacggagtga aaatcatctc tgtaggggtg cagaaagctt ctgaggaaaa cctgaaggcc
      781 atggccacgt ctcagtttca tttcaacctt cggacagtca gagacctcag catgttttcc
      841 caaaacatga cacacatcat caaggatgta ataaagtaca aggagggagc agttgatgac
      901 atctttgtag aagcttgcca aggcccttct atggccgatg ttgtgttcct attggatatg
      961 tcaatcaatg gaagtgagga gaactttgac tatcttaaag gattcttgga agaaagtgta
     1021 tctgcccttg acataaagga aaattgcatg agggttggcc ttgtggccta tagcaatgag
     1081 acaaaagtga taaattcact gagcatgggc ataaataagt cagaggttct ccagcatata
     1141 cagaaccttt ctccccgaac tgggaaggcc tatactggag ctgccatcaa aaagctcagg
     1201 aaggaagttt ttagtgcacg gaatggcagt cggaagaatc agggggtgcc ccagattgcc
     1261 gtgctggtga cccaccgaga ttcagaagac aacgtgacaa aagcagctgt taacctccga
     1321 cgggagggtg tgaccatctt caccctgggc atagagggcg ccagcgacac ccagttggaa
     1381 aagatagcat cccaccctgc tgagcagtat gtctccaaac tgaagacctt cgctgacctg
     1441 gctgctcaca accagacatt tctgaagaag ctgcggaacc aaataacaca cacagtctct
     1501 gtcttttcag agaggactga aacgctcaaa tctggttgtg tggacactga ggaagcagac
     1561 atctatctgc ttatcgatgg ctcagggagc acccaggcca cagatttcca tgaaatgaag
     1621 acgttcctgt cagaggtggt agggatgttc aacattgctc cccataaggt gcgggttggg
     1681 gccgttcagt atgctgacag ctgggacttg gaatttgaga tcaataaata ctccaacaag
     1741 caggatttgg gaaaggccat tgagaatatc aggcagatgg gtgggaatac aaacacaggc
     1801 gcagcactga atttcacact gagtctgttg caaaaagcaa agaagcagcg aggaaacaaa
     1861 gttccatgcc accttgttgt cctgacaaat ggcatgtcca aggatagcat cttggagcct
     1921 gcaaacagac tgagagaaga gcacatccga gtttatgcta tcgggatcaa ggaggccaac
     1981 caaacacagc tgagagaaat tgcaggagag gaaaagagag tgtattacgt gcatgacttt
     2041 gatgcattga aagacataag aaaccaagtt gttcaagaaa tctgtactga agaagcttgc
     2101 aaagagatga aagctgacat catgtttctg gtggacagtt ctggaagtat aggacctgaa
     2161 aacttcagca aaatgaaaac atttatgaaa aacctggtga gcaagtctca gattggacca
     2221 gatcgggtgc aaattggtgt agtccagttc agcgacatca ataaggaaga gtttcagctc
     2281 aacagattca tgtcccaaag cgacatttca aatgcaatag accaaatggc tcacattgga
     2341 caaaccaccc tgactggtag tgccctgagc tttgtgtctc agtacttcag ccccaccaag
     2401 ggcgcccggc ccaacatcag aaagtttctc atcctcatca cggatggtga agctcaggac
     2461 atagtaaagg aaccagcagt agtgcttcgg caagaaggtg taatcatcta ttctgtggga
     2521 gtgtttggct ccaatgtcac ccagcttgag gagatcagtg ggaggcccga gatggttttt
     2581 tatgttgaga attttgacat tctgcagcgc attgaagatg atcttgtttt tggaatatgc
     2641 agcccccgtg aagaatgcaa gcggattgaa gttttagacg ttgtgtttgt cattgatagc
     2701 tctggcagta ttgactatga tgagtataat atcatgaagg attttatgat tggcttagtg
     2761 aaaaaagctg atgtgggcaa gaatcaggtc cggtttgggg ctctgaagta tgctgatgac
     2821 ccagaggtgc tgttttatct ggatgacttt ggcacaaaac tggaggtaat ttcagtgctc
     2881 cagaatgacc aagccatggg tggcagtact tatactgctg aggcactggg cttctcagac
     2941 cacatgttca ctgaagcccg gggcagccgc ctgaacaagg gggtccccca agtcctcatt
     3001 gtgatcaccg atggggaatc ccatgatgct gataaactca atgccacggc aaaggccttg
     3061 cgggacaaag gcattcttgt cctggctgtg gggattgatg gtgccaatcc cgtggagctg
     3121 ttagccatgg caggatcaag cgacaagtac ttcttcgtgg agacttttgg aggtctgaag
     3181 ggaatatttt cagatgtgac agccagtgtc tgcaactctt caaaagtaga ttgtgaaatt
     3241 gacaaagtag atcttgtttt ccttatggat ggttcaacta gcattcagcc aaatgacttc
     3301 aagaaaatga aggaatttct ggcatctgtt gttcaagact ttgatgtcag cctcaacaga
     3361 gtgcgaatag gagcggccca gtttagcgat acctatcacc cggagtttcc actgggaact
     3421 ttcataggtg aaaaagagat atcatttcag attgaaaaca tcaagcagat ctttggaaac
     3481 acacacatcg gtgctgcact cagggaggtg gaacattact tcaggccaga catgggcagc
     3541 aggataaata caggtacccc acaggtgctg ctggtcctta cagatggcca gtcccaagac
     3601 gaggtggccc aggccgcgga agccctgaga cacagaggta tcgacatcta ctccgtgggc
     3661 attggggatg tggatgacca gcagctcatt cagatcaccg ggactgcaga gaaaaaactg
     3721 acagtgcaca acttcgatga actgaagaag gtcaataaaa ggatcgttcg caacatctgt
     3781 accacagcgg gtgaaagcaa ctgtttcgtg gatgttgtgg tgggatttga tgtctcaact
     3841 caggagaaag ggcagacttt gcttgaaggt cagccttgga tggaaaccta ccttcaagac
     3901 atcttacgtg ccatcagctc cctcaatgga gtaagctgtg aggtgggcac agagactcag
     3961 gtcagtgtgg cttttcaagt gaccaatgcc atggaaaaat attctcccaa gtttgagatc
     4021 tacagtgaaa acatactgaa tagcttgaag gatataacag ttaaaggacc atctcttctc
     4081 aatgcaaacc tcttggattc tctatgggat acatttcaga ataaatcagc tgctcgagga
     4141 aaggtggtcc ttttattttc agatggattg gatgatgatg ttgagaaact tgaacaaaaa
     4201 tctgatgaac ttagaaaaga aggcctgaat gccctcataa ctgttgctct ggatggacct
     4261 gctgattcaa gtgacttggc tgatcttccc tatattgaat ttgggaaagg atttgagtac
     4321 aggacacagc tctctattgg catgagagaa cttggaagcc ggctgtcaaa gcagctggtc
     4381 aatgttgctg aaaggacatg ctgctgtttg ttctgcaagt gcattggagg agatggcaca
     4441 atgggagatc ctggaccacc agggaaaagg ggacctccag gttttaaagg cagtgaaggc
     4501 tacctgggag aggagggaat cgctggagaa agaggagccc ctggaccagt gggagagcaa
     4561 ggtactaagg gatgctatgg caccaaaggt cctaagggaa acagaggact aaatggacag
     4621 gagggagaag ttggggaaaa tggaattgac ggattaaacg gagaacaggg tgataatggt
     4681 cttcctggaa gaaaaggaga aaagggagat gagggatctc agggaagccc agggaagaga
     4741 gggactcctg gtgaccgtgg agcaaagggc ctgcgagggg atcccggagc tcctggagtt
     4801 gacagtagca tagaaggacc cacaggcttg aaaggagaac gtggaagaca aggcagaaga
     4861 ggctggccag gcccccccgg gacaccaggc tccagaagaa agacagcagc tcatggcaga
     4921 aggggacata caggcccaca gggaacagca ggcatcccag gaccagatgg acttgaaggc
     4981 tccctgggac ttaagggccc tcagggccca agaggagagg ctggtgtgaa aggagaaaaa
     5041 ggaggtgtgg gaagtaaagg tccccagggg cctccaggac ccggaggaga ggcagggaat
     5101 caaggccgtt tgggaagcca aggaaataaa ggagaacctg gagatctggg agaaaaagga
     5161 gctgttggct ttcctggtcc tcgtggcttg cagggcaatg atggcagtcc aggttatggt
     5221 agtgtcggac gcaagggagc aaagggacaa gaaggattcc ctggagaaag tggacctaag
     5281 ggtgagattg gggaccctgg tggtccagga gagactgggc tgaagggagc tagaggcaaa
     5341 atgatatctg ctgggcttcc aggagagatg ggatcccctg gggaaccagg acctcctgga
     5401 cgtaagggtg tgaaaggagc caaaggcttg gcttcatttt ctacatgtga gctcattcag
     5461 tatgtgcgag accgcagtcc tggcaggcat ggaaaaccgg aatgcccagt gcacccaacc
     5521 gagttggtgt ttgccctgga ccactcccgg gatgtcactg agcaggaatt tgagcggatg
     5581 aaggagatga tggctttcct ggtgagagac attaaggtcc gggagaacag ctgccccgtg
     5641 ggagcgcaca tcgccatcct ctcctataac tcccacgcca ggcaccttgt gcgcttctca
     5701 gacgcctaca agaagagtca acttctcaga gaaattgaaa ctattcctta tgagagatcc
     5761 tctgccagca gggagattgg cagagcaatg cggtttattt ccaggaatgt cttcaagcgg
     5821 acgcttccgg gggcacacac gagaaaaatc gccacatttt tcagcagcgg tcagtccgcg
     5881 gatgcccact ccatcaccac ggctgccatg gagttcggcg cgcttgaaat cattcccgtg
     5941 gtgatcactt tcagcaacgt gccctcggtc aggcgcgcat ttgcgattga cgacactggc
     6001 acatttcaag taatagtggt tccctccggg gccgactaca taccagcatt agagagactc
     6061 cagcggtgca ctttctgcta tgatgtgtgc aagccagatg cttcttgtga ccaagccaga
     6121 ccaccccctg tgcagtctta catggatgct gctttccttc tggatgcctc ccggaacatg
     6181 ggaagtgctg aatttgaaga cataagagcc ttccttggag cactattaga tcactttgaa
     6241 atcaccccag agccggagac ttctgtcact ggagaccggg tggccctatt gagccatgct
     6301 ccccccgact tcctacccaa cactcagaag agtccagtta gagctgagtt caatcttacc
     6361 acctacagaa gtaagcgcct catgaagagg catgtgcacg agtcagttaa acaactaaat
     6421 ggagatgctt ttattggtca tgccttacag tggactctgg acaatgtatt tttaagtaca
     6481 cccaatctga gaagaaacaa agtcatattt gtgatatctg ctggggaaac cagccactta
     6541 gatggggaaa tcttaaagaa ggaatccttg cgagccaaat gtcagggata tgccttattt
     6601 gtgttttccc ttggccctat ttgggatgac aaggaactgg aggatctcgc cagccaccct
     6661 ttggatcacc acctggtcca gcttggccga attcataaac ctgaccacag ttatggtgtg
     6721 aagtttgtga agtcctttat aaactcaatc aggcgtgcaa tcaacaaata tccaccaata
     6781 aacttaaaaa taaagtgcaa cagacttaac tctatagatc caaagcagcc cccacgacca
     6841 ttccgaagct ttgttcctgg accacttaaa gctaccctca aagaagatgt attacagaag
     6901 gcaaaattct ttcaagataa aaaatatctt tcaagagtag caagaagtgg cagagatgat
     6961 gctattcaaa attttatgag aagcacctcc cataccttta agaatggaag gatgatagaa
     7021 agtgctccca aacaacatga ttaaaaaaat gcttgaacaa cttagcctta ggaagcatgg
     7081 taagactctg gacttaaata gtaactaaat ctgctgccag aactcaagca acagtttgta
     7141 ggttatcagg tgacttgacc ccctgcattc attggtatta agatatatct tgttcattta
     7201 tttgaccact cctgacaatt ccagcactct acgactgata tgtcgaagaa ctgtttcatt
     7261 agaagacaga agaatgaaag aagtgttttg aaaagtctaa tggagatata atttgaaggt
     7321 aaaatttata tgatgtatgc atatgtgtac atgggtcaat gttaattctt ttatctctgt
     7381 ccagttcatt ttctgcaaac ccatcctcct ttcctaattt agtaatgtct aatgctcatc
     7441 tatttagtca aagttatttt ttaatgtttt aagctactcc aaaatttatc ctagaaatgg
     7501 atgctgttta tatgtcgtat ttttttaaaa cgtgaccttc tattccactt aaacacacca
     7561 ctgtgggagt tgtttgcttg gactgatgag aagagggtaa ttttctccat ctctaaatcc
     7621 tcctgaactc actgaaaaac tcattaattc ccgcagaaca taaattattg cttttgagct
     7681 ggaagttttc tcttcattag gaagcttact gccattttga tggagcttga agcccccatt
     7741 ttgatggact tctagtctgt ctttcctgtt atggtcattt attaattttc actaatccat
     7801 atattcttta gacaaggaga gtcaagaaac tacttgtcat agatgaagct ctgagttact
     7861 tttacccatc aggtagctgt ttagtcaata ataaacagca ccaaaacagg attagcagag
     7921 ctcttaaaaa cagccttaaa tacacactga gtttagaaaa atgtaagaaa atcattgttt
     7981 taatctacaa attcatatgc agttgcagag atgcaaaaga gatcagagta agttaaagta
     8041 ggaaaggttt tataaaagta ttgatggctg tgtcctcatc tgcatttttg tttttatgat
     8101 agggctgata ttttcaacaa cgaaacaccc aatgttcaga ccttacctca gtctaagagc
     8161 tggctccaca gttggtagca atggccctgt taggcagact gtccccctct ttgggaatgg
     8221 aaagaagccc tgcctggatg catggagcag atggatactg acaccaagga cattcagatt
     8281 ttctttccta tacctatttt cataaaattt ggaaagaaaa actatctgca gtattttttt
     8341 aaaacgactt ctgctattct tacttagaga aatccagtga tagcacctca tgtcttcaga
     8401 ggagcaagaa gttcaaggag ttaaatgtca cattcatact caggtaatag agaagaataa
     8461 aacacatcag gtttgcttca tctatcctaa gtggtgctta ggggttcaga aaaaatgttt
     8521 caagtacagg aaattaatga gcaatattta caatgtattt taataaaagg gatttttcta
     8581 gttaactctg agttaagcac gaactccttt cttttgttaa ttgttggaaa taccattaag
     8641 atgtatttgt ctgtttttct gcattggcaa gtaaagaaca taaaacaaa
//
