LOCUS       NM_001849               3445 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens collagen type VI alpha 2 chain (COL6A2), transcript
            variant 2C2, mRNA.
ACCESSION   NM_001849
VERSION     NM_001849.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Sharma,V., Vinchure,O.S., Yadav,G., Sarkar,C. and Kulshreshtha,R.
  TITLE     A novel interplay between PRC2 and miR-3189 regulates
            epithelial-mesenchymal transition (EMT) via modulating COL6A2 in
            glioblastoma
  JOURNAL   J Cell Physiol 239 (8), e31326 (2024)
   PUBMED   38860406
  REMARK    GeneRIF: A novel interplay between PRC2 and miR-3189 regulates
            epithelial-mesenchymal transition (EMT) via modulating COL6A2 in
            glioblastoma.
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Zanotti,S., Magri,F., Salani,S., Napoli,L., Ripolone,M., Ronchi,D.,
            Fortunato,F., Ciscato,P., Velardo,D., D'Angelo,M.G., Gualandi,F.,
            Nigro,V., Sciacco,M., Corti,S., Comi,G.P. and Piga,D.
  TITLE     Extracellular Matrix Disorganization and Sarcolemmal Alterations in
            COL6-Related Myopathy Patients with New Variants of COL6 Genes
  JOURNAL   Int J Mol Sci 24 (6), 5551 (2023)
   PUBMED   36982625
  REMARK    GeneRIF: Extracellular Matrix Disorganization and Sarcolemmal
            Alterations in COL6-Related Myopathy Patients with New Variants of
            COL6 Genes.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3445)
  AUTHORS   Hong,X., Zhang,J., Zou,J., Ouyang,J., Xiao,B., Wang,P. and Peng,X.
  TITLE     Role of COL6A2 in malignant progression and temozolomide resistance
            of glioma
  JOURNAL   Cell Signal 102, 110560 (2023)
   PUBMED   36521657
  REMARK    GeneRIF: Role of COL6A2 in malignant progression and temozolomide
            resistance of glioma.
REFERENCE   4  (bases 1 to 3445)
  AUTHORS   Ionova,S.A., Murtazina,A.F., Tebieva,I.S., Getoeva,Z.K.,
            Dadali,E.L., Chausova,P.A., Shchagina,O.A., Marakhonov,A.V.,
            Kutsev,S.I. and Zinchenko,R.A.
  TITLE     The Presentation of Two Unrelated Clinical Cases from the Republic
            of North Ossetia-Alania with the Same Previously Undescribed
            Variant in the COL6A2 Gene
  JOURNAL   Int J Mol Sci 23 (20), 12127 (2022)
   PUBMED   36292982
  REMARK    GeneRIF: The Presentation of Two Unrelated Clinical Cases from the
            Republic of North Ossetia-Alania with the Same Previously
            Undescribed Variant in the COL6A2 Gene.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3445)
  AUTHORS   Foley,A.R., Mohassel,P., Donkervoort,S., Bolduc,V. and
            Bonnemann,C.G.
  TITLE     Collagen VI-Related Dystrophies
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301676
REFERENCE   6  (bases 1 to 3445)
  AUTHORS   Sparks,S.E., Quijano-Roy,S., Harper,A., Rutkowski,A., Gordon,E.,
            Hoffman,E.P. and Pegoraro,E.
  TITLE     Congenital Muscular Dystrophy Overview - RETIRED CHAPTER, FOR
            HISTORICAL REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301468
REFERENCE   7  (bases 1 to 3445)
  AUTHORS   Dawson,S.J. and White,L.A.
  TITLE     Treatment of Haemophilus aphrophilus endocarditis with
            ciprofloxacin
  JOURNAL   J Infect 24 (3), 317-320 (1992)
   PUBMED   1602151
REFERENCE   8  (bases 1 to 3445)
  AUTHORS   Saitta,B., Timpl,R. and Chu,M.L.
  TITLE     Human alpha 2(VI) collagen gene. Heterogeneity at the
            5'-untranslated region generated by an alternate exon
  JOURNAL   J Biol Chem 267 (9), 6188-6196 (1992)
   PUBMED   1556127
REFERENCE   9  (bases 1 to 3445)
  AUTHORS   Bidanset,D.J., Guidry,C., Rosenberg,L.C., Choi,H.U., Timpl,R. and
            Hook,M.
  TITLE     Binding of the proteoglycan decorin to collagen type VI
  JOURNAL   J Biol Chem 267 (8), 5250-5256 (1992)
   PUBMED   1544908
REFERENCE   10 (bases 1 to 3445)
  AUTHORS   Saitta,B., Stokes,D.G., Vissing,H., Timpl,R. and Chu,M.L.
  TITLE     Alternative splicing of the human alpha 2(VI) collagen gene
            generates multiple mRNA transcripts which predict three protein
            variants with distinct carboxyl termini
  JOURNAL   J Biol Chem 265 (11), 6473-6480 (1990)
   PUBMED   1690728
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP001471.1, CN352155.1,
            BC065509.1, CN352095.1 and AY029208.1.
            
            On Aug 31, 2019 this sequence version replaced NM_001849.3.
            
            Summary: This gene encodes one of the three alpha chains of type VI
            collagen, a beaded filament collagen found in most connective
            tissues. The product of this gene contains several domains similar
            to von Willebrand Factor type A domains. These domains have been
            shown to bind extracellular matrix proteins, an interaction that
            explains the importance of this collagen in organizing matrix
            components. Mutations in this gene are associated with Bethlem
            myopathy and Ullrich scleroatonic muscular dystrophy. Three
            transcript variants have been identified for this gene. [provided
            by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2C2), that differs from the other
            variants in the last exon, encodes the full-length protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC065509.1, SRR14038194.2193221.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000300527.9/ ENSP00000300527.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-7                 AP001471.1         5660-5666
            8-139               CN352155.1         3-134
            140-1284            BC065509.1         119-1263
            1285-1509           CN352095.1         303-527
            1510-2812           BC065509.1         1489-2791
            2813-2986           AY029208.1         2745-2918
            2987-3067           BC065509.1         2966-3046
            3068-3445           AY029208.1         3000-3377
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="21"
                     /map="21q22.3"
     gene            1..3445
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /note="collagen type VI alpha 2 chain"
                     /db_xref="GeneID:1292"
                     /db_xref="HGNC:HGNC:2212"
                     /db_xref="MIM:120240"
     exon            1..62
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /inference="alignment:Splign:2.1.0"
     exon            63..204
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /inference="alignment:Splign:2.1.0"
     CDS             90..3149
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /note="isoform 2C2 precursor is encoded by transcript
                     variant 2C2; human mRNA for collagen VI alpha-2 C-terminal
                     globular domain; collagen VI, alpha-2 polypeptide;
                     collagen alpha-2(VI) chain; epididymis secretory sperm
                     binding protein; collagen, type VI, alpha 2"
                     /codon_start=1
                     /product="collagen alpha-2(VI) chain isoform 2C2
                     precursor"
                     /protein_id="NP_001840.3"
                     /db_xref="CCDS:CCDS13728.1"
                     /db_xref="GeneID:1292"
                     /db_xref="HGNC:HGNC:2212"
                     /db_xref="MIM:120240"
                     /translation="MLQGTCSVLLLWGILGAIQAQQQEVISPDTTERNNNCPEKTDCP
                     IHVYFVLDTSESVTMQSPTDILLFHMKQFVPQFISQLQNEFYLDQVALSWRYGGLHFS
                     DQVEVFSPPGSDRASFIKNLQGISSFRRGTFTDCALANMTEQIRQDRSKGTVHFAVVI
                     TDGHVTGSPCGGIKLQAERAREEGIRLFAVAPNQNLKEQGLRDIASTPHELYRNDYAT
                     MLPDSTEIDQDTINRIIKVMKHEAYGECYKVSCLEIPGPSGPKGYRGQKGAKGNMGEP
                     GEPGQKGRQGDPGIEGPIGFPGPKGVPGFKGEKGEFGADGRKGAPGLAGKNGTDGQKG
                     KLGRIGPPGCKGDPGNRGPDGYPGEAGSPGERGDQGGKGDPGRPGRRGPPGEIGAKGS
                     KGYQGNSGAPGSPGVKGAKGGPGPRGPKGEPGRRGDPGTKGSPGSDGPKGEKGDPGPE
                     GPRGLAGEVGNKGAKGDRGLPGPRGPQGALGEPGKQGSRGDPGDAGPRGDSGQPGPKG
                     DPGRPGFSYPGPRGAPGEKGEPGPRGPEGGRGDFGLKGEPGRKGEKGEPADPGPPGEP
                     GPRGPRGVPGPEGEPGPPGDPGLTECDVMTYVRETCGCCDCEKRCGALDVVFVIDSSE
                     SIGYTNFTLEKNFVINVVNRLGAIAKDPKSETGTRVGVVQYSHEGTFEAIQLDDERID
                     SLSSFKEAVKNLEWIAGGTWTPSALKFAYDRLIKESRRQKTRVFAVVITDGRHDPRDD
                     DLNLRALCDRDVTVTAIGIGDMFHEKHESENLYSIACDKPQQVRNMTLFSDLVAEKFI
                     DDMEDVLCPDPQIVCPDLPCQTELSVAQCTQRPVDIVFLLDGSERLGEQNFHKARRFV
                     EQVARRLTLARRDDDPLNARVALLQFGGPGEQQVAFPLSHNLTAIHEALETTQYLNSF
                     SHVGAGVVHAINAIVRSPRGGARRHAELSFVFLTDGVTGNDSLHESAHSMRKQNVVPT
                     VLALGSDVDMDVLTTLSLGDRAAVFHEKDYDSLAQPGFFDRFIRWIC"
     exon            205..803
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /inference="alignment:Splign:2.1.0"
     exon            804..824
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /inference="alignment:Splign:2.1.0"
     exon            825..890
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /inference="alignment:Splign:2.1.0"
     exon            891..944
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /inference="alignment:Splign:2.1.0"
     exon            945..989
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /inference="alignment:Splign:2.1.0"
     exon            990..1016
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1017..1043
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1044..1088
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1089..1142
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1143..1205
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1206..1268
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1269..1358
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1359..1421
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1422..1484
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1485..1547
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1548..1610
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1611..1661
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1662..1697
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1698..1760
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1761..1823
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1824..1859
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1860..1905
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1906..2058
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /inference="alignment:Splign:2.1.0"
     exon            2059..2511
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /inference="alignment:Splign:2.1.0"
     exon            2512..2550
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /inference="alignment:Splign:2.1.0"
     exon            2551..3445
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3427..3432
                     /regulatory_class="polyA_signal_sequence"
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /note="hexamer: AATAAA"
     polyA_site      3445
                     /gene="COL6A2"
                     /gene_synonym="BTHLM1; BTHLM1B; PP3610; UCMD1; UCMD1B"
                     /note="major polyA site"
ORIGIN      
        1 ccctgctgct tactcggcgc ccgcgcctcg ggccgtcggg agcggagcct cctcgggacc
       61 aggacttcag ggccacaggt gctgccaaga tgctccaggg cacctgctcc gtgctcctgc
      121 tctggggaat cctgggggcc atccaggccc agcagcagga ggtcatctcg ccggacacta
      181 ccgagagaaa caacaactgc ccagagaaga ccgactgccc catccacgtg tacttcgtgc
      241 tggacacctc ggagagcgtc accatgcagt cccccacgga catcctgctc ttccacatga
      301 agcagttcgt gccgcagttc atcagccagc tgcagaacga gttctacctg gaccaggtgg
      361 cgctgagctg gcgctacggc ggcctgcact tctctgacca ggtggaggtg ttcagcccac
      421 cgggcagcga ccgggcctcc ttcatcaaga acctgcaggg catcagctcc ttccgccgcg
      481 gcaccttcac cgactgcgcg ctggccaaca tgacggagca gatccggcag gaccgcagca
      541 agggcaccgt ccacttcgcc gtggtcatca ccgacggcca cgtcaccggc agcccctgcg
      601 ggggcatcaa gctgcaggcc gagcgggccc gcgaggaggg catccggctc ttcgccgtgg
      661 cccccaacca gaacctgaag gagcagggcc tgcgggacat cgccagcacg ccgcacgagc
      721 tctaccgcaa cgactacgcc accatgctgc ccgactccac cgagatcgac caggacacca
      781 tcaaccgcat catcaaggtc atgaaacacg aagcctacgg agagtgctac aaggtgagct
      841 gcctggaaat ccctgggccc tctggcccca agggctaccg tggacagaag ggtgccaagg
      901 gcaacatggg tgagccggga gagcctggcc agaagggaag acagggagac ccgggcatcg
      961 aaggccccat tggattccca ggacccaagg gcgttcctgg cttcaaagga gagaagggtg
     1021 aatttggagc cgacggtcgc aagggggccc ctggcctggc tggcaagaac gggaccgatg
     1081 gacagaaggg caagctgggg cgcatcggac ctcctggctg caagggagac cctggaaacc
     1141 ggggccccga cggttacccg ggggaagcag ggagtccagg ggagcgagga gaccaaggcg
     1201 gcaaggggga ccctggccgc ccaggacgca gagggccccc gggagaaatc ggggccaagg
     1261 gaagcaaggg gtatcaaggc aacagtggag ccccaggaag tcctggtgtg aaaggagcca
     1321 agggcgggcc tgggccccgc ggacccaaag gcgagccggg gcgcagggga gaccccggca
     1381 ccaagggcag cccaggcagc gatggcccca agggggagaa gggggaccct ggccctgagg
     1441 ggccccgcgg cctggctgga gaggttggca acaaaggagc caagggagac cgaggcttgc
     1501 ctggacccag aggcccccag ggagctcttg gggagcccgg aaagcaggga tctcggggag
     1561 accccggtga tgcaggaccc cgtggagact caggacagcc aggccccaag ggagaccccg
     1621 gcaggcctgg attcagctac ccaggacccc gaggagcacc cggagaaaaa ggcgagcccg
     1681 gcccacgcgg ccccgaggga ggccgaggcg actttggctt gaaaggagaa cctgggagga
     1741 aaggagagaa aggagagcct gcggatcctg gtccccctgg tgagccaggc cctcgggggc
     1801 caagaggagt cccaggaccc gagggtgagc ccggcccccc tggagacccc ggtctcacgg
     1861 agtgtgacgt catgacctac gtgagggaga cctgcgggtg ctgcgactgt gagaagcgct
     1921 gtggcgccct ggacgtggtc ttcgtcatcg acagctccga gagcattggg tacaccaact
     1981 tcacactgga gaagaacttc gtcatcaacg tggtcaacag gctgggtgcc atcgctaagg
     2041 accccaagtc cgagacaggg acgcgtgtgg gcgtggtgca gtacagccac gagggcacct
     2101 ttgaggccat ccagctggac gacgaacgta tcgactccct gtcgagcttc aaggaggctg
     2161 tcaagaacct cgagtggatt gcgggcggca cctggacacc ctcagccctc aagtttgcct
     2221 acgaccgcct catcaaggag agccggcgcc agaagacacg tgtgtttgcg gtggtcatca
     2281 cggacgggcg ccacgaccct cgggacgatg acctcaactt gcgggcgctg tgcgaccgcg
     2341 acgtcacagt gacggccatc ggcatcgggg acatgttcca cgagaagcac gagagtgaaa
     2401 acctctactc catcgcctgc gacaagccac agcaggtgcg caacatgacg ctgttctccg
     2461 acctggtcgc tgagaagttc atcgatgaca tggaggacgt cctctgcccg gaccctcaga
     2521 tcgtgtgccc agaccttccc tgccaaacag agctgtccgt ggcacagtgc acgcagcggc
     2581 ccgtggacat cgtcttcctg ctggacggct ccgagcggct gggtgagcag aacttccaca
     2641 aggcccggcg cttcgtggag caggtggcgc ggcggctgac gctggcccgg agggacgacg
     2701 accctctcaa cgcacgcgtg gcgctgctgc agtttggtgg ccccggcgag cagcaggtgg
     2761 ccttcccgct gagccacaac ctcacggcca tccacgaggc gctggagacc acacaatacc
     2821 tgaactcctt ctcgcacgtg ggcgcaggcg tggtgcacgc catcaatgcc atcgtgcgca
     2881 gcccgcgtgg cggggcccgg aggcacgcag agctgtcctt cgtgttcctc acggacggcg
     2941 tcacgggcaa cgacagtctg cacgagtcgg cgcactccat gcgcaagcag aacgtggtac
     3001 ccaccgtgct ggccttgggc agcgacgtgg acatggacgt gctcaccacg ctcagcctgg
     3061 gtgaccgcgc cgccgtgttc cacgagaagg actatgacag cctggcgcaa cccggcttct
     3121 tcgaccgctt catccgctgg atctgctagc gccgccgccc gggccccgca gtcgagggtc
     3181 gtgagcccac cccgtccatg gtgctaagcg ggcccgggtc ccacacggcc agcaccgctg
     3241 ctcactcgga cgacgccctg ggcctgcacc tctccagctc ctcccacggg gtccccgtag
     3301 ccccggcccc cgcccagccc caggtctccc caggccctcc gcaggctgcc cggcctccct
     3361 ccccctgcag ccatcccaag gctcctgacc tacctggccc ctgagctctg gagcaagccc
     3421 tgacccaata aaggctttga accca
//
