LOCUS       NM_033380               6531 bp    mRNA    linear   PRI 11-OCT-2024
DEFINITION  Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript
            variant 2, mRNA.
ACCESSION   NM_033380
VERSION     NM_033380.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6531)
  AUTHORS   Wang,D., Pan,M., Li,H., Li,M., Li,P., Xiong,F. and Xiao,H.
  TITLE     Four novel mutations identified in the COL4A3, COL4A4 and COL4A5
            genes in 10 families with Alport syndrome
  JOURNAL   BMC Med Genomics 17 (1), 181 (2024)
   PUBMED   38978054
  REMARK    GeneRIF: Four novel mutations identified in the COL4A3, COL4A4 and
            COL4A5 genes in 10 families with Alport syndrome.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6531)
  AUTHORS   Chen,S., Xu,G., Zhao,Z., Du,J., Shen,B. and Li,C.
  TITLE     A novel COL4A5 splicing mutation causes alport syndrome in a
            Chinese family
  JOURNAL   BMC Med Genomics 17 (1), 108 (2024)
   PUBMED   38671472
  REMARK    GeneRIF: A novel COL4A5 splicing mutation causes alport syndrome in
            a Chinese family.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6531)
  AUTHORS   Xiao,T., Zhang,J., Liu,L. and Zhang,B.
  TITLE     Genetic diagnosis of Alport syndrome in 16 Chinese families
  JOURNAL   Mol Genet Genomic Med 12 (3), e2406 (2024)
   PUBMED   38433557
  REMARK    GeneRIF: Genetic diagnosis of Alport syndrome in 16 Chinese
            families.
REFERENCE   4  (bases 1 to 6531)
  AUTHORS   Zhang,R., Lang,Y., Shi,X., Zhang,Y., Liu,X., Pan,F., Qiao,D.,
            Teng,X. and Shao,L.
  TITLE     Three exonic variants in the COL4A5 gene alter RNA splicing in a
            minigene assay
  JOURNAL   Mol Genet Genomic Med 12 (2), e2395 (2024)
   PUBMED   38400605
  REMARK    GeneRIF: Three exonic variants in the COL4A5 gene alter RNA
            splicing in a minigene assay.
REFERENCE   5  (bases 1 to 6531)
  AUTHORS   Kashtan,C.E.
  TITLE     Alport Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301386
REFERENCE   6  (bases 1 to 6531)
  AUTHORS   Milewicz,D.M. and Cecchi,A.C.
  TITLE     Heritable Thoracic Aortic Disease Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301299
REFERENCE   7  (bases 1 to 6531)
  AUTHORS   Knebelmann,B., Deschenes,G., Gros,F., Hors,M.C., Grunfeld,J.P.,
            Zhou,J., Tryggvason,K., Gubler,M.C. and Antignac,C.
  TITLE     Substitution of arginine for glycine 325 in the collagen alpha 5
            (IV) chain associated with X-linked Alport syndrome:
            characterization of the mutation by direct sequencing of
            PCR-amplified lymphoblast cDNA fragments
  JOURNAL   Am J Hum Genet 51 (1), 135-142 (1992)
   PUBMED   1376965
  REMARK    Erratum:[Am J Hum Genet 1992 Oct;51(4):942]
REFERENCE   8  (bases 1 to 6531)
  AUTHORS   Zhou,J., Hertz,J.M., Leinonen,A. and Tryggvason,K.
  TITLE     Complete amino acid sequence of the human alpha 5 (IV) collagen
            chain and identification of a single-base mutation in exon 23
            converting glycine 521 in the collagenous domain to cysteine in an
            Alport syndrome patient
  JOURNAL   J Biol Chem 267 (18), 12475-12481 (1992)
   PUBMED   1352287
REFERENCE   9  (bases 1 to 6531)
  AUTHORS   Ghebrehiwet,B., Peerschke,E.I., Hong,Y., Munoz,P. and Gorevic,P.D.
  TITLE     Short amino acid sequences derived from C1q receptor (C1q-R) show
            homology with the alpha chains of fibronectin and vitronectin
            receptors and collagen type IV
  JOURNAL   J Leukoc Biol 51 (6), 546-556 (1992)
   PUBMED   1377218
REFERENCE   10 (bases 1 to 6531)
  AUTHORS   Renieri,A., Seri,M., Myers,J.C., Pihlajaniemi,T., Massella,L.,
            Rizzoni,G. and De Marchi,M.
  TITLE     De novo mutation in the COL4A5 gene converting glycine 325 to
            glutamic acid in Alport syndrome
  JOURNAL   Hum Mol Genet 1 (2), 127-129 (1992)
   PUBMED   1363780
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL034369.1, DA461056.1,
            BC151846.1, BC035387.1, M31115.1 and BU688037.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_033380.2.
            
            Summary: This gene encodes one of the six subunits of type IV
            collagen, the major structural component of basement membranes.
            Mutations in this gene are associated with X-linked Alport
            syndrome, also known as hereditary nephritis. Like the other
            members of the type IV collagen gene family, this gene is organized
            in a head-to-head conformation with another type IV collagen gene
            so that each gene pair shares a common promoter. Alternatively
            spliced transcript variants have been identified for this gene.
            [provided by RefSeq, Aug 2010].
            
            Transcript Variant: This variant (2) encodes the longer isoform
            (2).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC151846.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000328300.11/ ENSP00000331902.7
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6                 AL034369.1         58846-58851
            7-44                DA461056.1         1-38
            45-2413             BC151846.1         1-2369
            2414-3534           BC035387.1         1583-2703
            3535-3869           BC151846.1         3491-3825
            3870-4078           M31115.1           843-1051
            4079-5896           BC151846.1         4035-5852
            5897-6531           BU688037.1         17-651              c
FEATURES             Location/Qualifiers
     source          1..6531
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq22.3"
     gene            1..6531
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /note="collagen type IV alpha 5 chain"
                     /db_xref="GeneID:1287"
                     /db_xref="HGNC:HGNC:2207"
                     /db_xref="MIM:303630"
     exon            1..369
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     CDS             289..5364
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; collagen of basement membrane, alpha-5 chain;
                     collagen IV, alpha-5 polypeptide; collagen alpha-5(IV)
                     chain; dA149D17.3; collagen, type IV, alpha 5; dA24A23.1"
                     /codon_start=1
                     /product="collagen alpha-5(IV) chain isoform 2 precursor"
                     /protein_id="NP_203699.1"
                     /db_xref="CCDS:CCDS35366.1"
                     /db_xref="GeneID:1287"
                     /db_xref="HGNC:HGNC:2207"
                     /db_xref="MIM:303630"
                     /translation="MKLRGVSLAAGLFLLALSLWGQPAEAAACYGCSPGSKCDCSGIK
                     GEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPG
                     TPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGS
                     IIMSSLPGPKGNPGYPGPPGIQGLPGPTGIPGPIGPPGPPGLMGPPGPPGLPGPKGNM
                     GLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRG
                     PPGPPGGEKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKG
                     DTGPPGPPGLVIPRPGTGITIGEKGNIGLPGLPGEKGERGFPGIQGPPGLPGPPGAAV
                     MGPPGPPGFPGERGQKGDEGPPGISIPGPPGLDGQPGAPGLPGPPGPAGPHIPPSDEI
                     CEPGPPGPPGSPGDKGLQGEQGVKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSL
                     GFPGQKGEKGQAGATGPKGLPGIPGAPGAPGFPGSKGEPGDILTFPGMKGDKGELGSP
                     GAPGLPGLPGTPGQDGLPGLPGPKGEPGGITFKGERGPPGNPGLPGLPGNIGPMGPPG
                     FGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGNPGRDGDVGLPGD
                     PGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGTPGRIGLEGPPG
                     PPGFPGPKGEPGFALPGPPGPPGLPGFKGALGPKGDRGFPGPPGPPGRTGLDGLPGPK
                     GDVGPNGQPGPMGPPGLPGIGVQGPPGPPGIPGPIGQPGLHGIPGEKGDPGPPGLDVP
                     GPPGERGSPGIPGAPGPIGPPGSPGLPGKAGASGFPGTKGEMGMMGPPGPPGPLGIPG
                     RSGVPGLKGDDGLQGQPGLPGPTGEKGSKGEPGLPGPPGPMDPNLLGSKGEKGEPGLP
                     GIPGVSGPKGYQGLPGDPGQPGLSGQPGLPGPPGPKGNPGLPGQPGLIGPPGLKGTIG
                     DMGFPGPQGVEGPPGPSGVPGQPGSPGLPGQKGDKGDPGISSIGLPGLPGPKGEPGLP
                     GYPGNPGIKGSVGDPGLPGLPGTPGAKGQPGLPGFPGTPGPPGPKGISGPPGNPGLPG
                     EPGPVGGGGHPGQPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGNPGPPGLPGLSGQK
                     GDGGLPGIPGNPGLPGPKGEPGFHGFPGVQGPPGPPGSPGPALEGPKGNPGPQGPPGR
                     PGPTGFQGLPGPEGPPGLPGNGGIKGEKGNPGQPGLPGLPGLKGDQGPPGLQGNPGRP
                     GLNGMKGDPGLPGVPGFPGMKGPSGVPGSAGPEGEPGLIGPPGPPGLPGPSGQSIIIK
                     GDAGPPGIPGQPGLKGLPGPQGPQGLPGPTGPPGDPGRNGLPGFDGAGGRKGDPGLPG
                     QPGTRGLDGPPGPDGLQGPPGPPGTSSVAHGFLITRHSQTTDAPQCPQGTLQVYEGFS
                     LLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMP
                     MSMQPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTS
                     AGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVSDMFSKPQ
                     SETLKAGDLRTRISRCQVCMKRT"
     exon            370..429
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            430..519
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            520..564
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            565..609
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            610..672
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            673..726
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            727..753
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            754..834
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            835..897
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            898..933
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            934..975
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            976..1068
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            1069..1122
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            1123..1179
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            1180..1224
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            1225..1278
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            1279..1320
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            1321..1453
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            1454..1627
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            1628..1711
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            1712..1804
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            1805..1875
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            1876..2067
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            2068..2236
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            2237..2329
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            2330..2434
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            2435..2532
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            2533..2683
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            2684..2797
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            2798..2965
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            2966..3055
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            3056..3205
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            3206..3304
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            3305..3394
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            3395..3534
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            3535..3661
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            3662..3742
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            3743..3841
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            3842..3892
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            3893..4078
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            4079..4087
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            4088..4096
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            4097..4230
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            4231..4303
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            4304..4375
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            4376..4504
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            4505..4603
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            4604..4816
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            4817..4994
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            4995..5109
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            5110..5282
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     exon            5283..6531
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6508..6513
                     /regulatory_class="polyA_signal_sequence"
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /note="hexamer: ATTAAA"
     polyA_site      6531
                     /gene="COL4A5"
                     /gene_synonym="ASLN; ATS; ATS1; CA54"
                     /note="major polyA site"
ORIGIN      
        1 agatctgtag gaattgagtg aagaaaaagt ttgcaagtct ggacagaagg gaaaagttct
       61 cctgagagac cggctttggg gaggggaggg gggaaggaag agtagctcct tcttcttctt
      121 cttttttttt tcttccactc ttaaaaagct tctttctctt cacccaagcc tcactgtccc
      181 tctccggctc tagctctctc catataaacc ctcaagatta tgtcaattgg ttagagccag
      241 ccgggaattt cgtgcgggtg ctgaaggagc tgcgggagcc ggagaagaat gaaactgcgt
      301 ggagtcagcc tggctgccgg cttgttctta ctggccctga gtctttgggg gcagcctgca
      361 gaggctgcgg cttgctatgg gtgttctcca ggatcaaagt gtgactgcag tggcataaaa
      421 ggggaaaagg gagagagagg gtttccaggt ttggaaggac acccaggatt gcctggattt
      481 ccaggtccag aagggcctcc ggggcctcgg ggacaaaagg gtgatgatgg aattccaggg
      541 ccaccaggac caaaaggaat cagaggtcct cctggacttc ctggatttcc agggacacca
      601 ggtcttcctg gaatgccagg ccacgatggg gccccaggac ctcaaggtat tcccggatgc
      661 aatggaacca agggagaacg tggatttcca ggcagtcccg gttttcctgg tttacagggt
      721 cctccaggac cccctgggat cccaggtatg aagggtgaac caggtagtat aattatgtca
      781 tcactgccag gaccaaaggg taatccagga tatccaggtc ctcctggaat acaaggccta
      841 cctggtccca ctggtatacc agggccaatt ggtcccccag gaccaccagg tttgatgggc
      901 cctcctggtc caccaggact tccaggacct aaggggaata tgggcttaaa tttccaggga
      961 cccaaaggtg aaaaaggtga gcaaggtctt cagggcccac ctgggccacc tgggcagatc
     1021 agtgaacaga aaagaccaat tgatgtagag tttcagaaag gagatcaggg acttcctggt
     1081 gaccgagggc ctcctggacc tccagggata cgtggtcctc caggtccccc aggtggtgag
     1141 aaaggtgaga agggtgagca aggagagcca ggcaaaagag gtaaaccagg caaagatgga
     1201 gaaaatggcc aaccaggaat tcctggtttg cctggtgatc ctggttaccc tggtgaaccc
     1261 ggaagggatg gtgaaaaggg ccaaaaaggt gacactggcc cacctggacc tcctggactt
     1321 gtaattccta gacctgggac tggtataact ataggagaaa aaggaaacat tgggttgcct
     1381 gggttgcctg gagaaaaagg agagcgagga tttcctggaa tacagggtcc acctggcctt
     1441 cctggacctc caggggctgc agttatgggt cctcctggcc ctcctggatt tcctggagaa
     1501 aggggtcaga aaggtgatga aggaccacct ggaatttcca ttcctggacc tcctggactt
     1561 gacggacagc ctggggctcc tgggcttcca gggcctcctg gccctgctgg ccctcacatt
     1621 cctcctagtg atgagatatg tgaaccaggc cctccaggcc ccccaggatc tccaggtgat
     1681 aaaggactcc aaggagaaca aggagtgaaa ggtgacaaag gtgacacttg cttcaactgc
     1741 attggaactg gtatttcagg gcctccaggt caacctggtt tgccaggtct cccaggtcct
     1801 ccaggatctc ttggtttccc tggacagaaa ggggaaaaag gacaagctgg tgcaactggt
     1861 cccaaaggat taccaggcat tccaggagct ccaggtgctc caggctttcc tggatctaaa
     1921 ggtgaacctg gtgatatcct cacttttcca ggaatgaagg gtgacaaagg agagttgggt
     1981 tcccctggag ctccagggct tcctggttta cctggcactc ctggacagga tggattgcca
     2041 gggcttcctg gcccgaaagg agagcctggt ggaattactt ttaagggtga aagaggtccc
     2101 cctgggaacc caggtttacc aggcctccca gggaatatag ggcctatggg tccccctggt
     2161 ttcggccctc caggcccagt aggtgaaaaa ggcatacaag gtgtggcagg aaatccaggc
     2221 cagccaggaa taccaggtcc taaaggggat ccaggtcaga ctataaccca gccggggaag
     2281 cctggcttgc ctggtaaccc aggcagagat ggtgatgtag gtcttccagg tgaccctgga
     2341 cttccagggc aaccaggctt gccagggata cctggtagca aaggagaacc aggtatccct
     2401 ggaattgggc ttcctggacc acctggtccc aaaggctttc ctggaattcc aggacctcca
     2461 ggagcacctg ggacacctgg aagaattggt ctagaaggcc ctcctgggcc acccggcttt
     2521 ccaggaccaa agggtgaacc aggatttgca ttacctgggc cacctgggcc accaggactt
     2581 ccaggtttca aaggagcact tggtccaaaa ggtgatcgtg gtttcccagg acctccgggt
     2641 cctccaggac gcactggctt agatgggctc cctggaccaa aaggtgatgt tggaccaaat
     2701 ggacaacctg gaccaatggg acctcctggg ctgccaggaa taggtgttca gggaccacca
     2761 ggaccaccag ggattcctgg gccaataggt caacctggtt tacatggaat accaggagag
     2821 aagggggatc caggacctcc tggacttgat gttccaggac ccccaggtga aagaggcagt
     2881 ccagggatcc ccggagcacc tggtcctata ggacctccag gatcaccagg gcttccagga
     2941 aaagcaggtg cctctggatt tccaggtacc aaaggtgaaa tgggtatgat gggacctcca
     3001 ggcccaccag gacctttggg aattcctggc aggagtggtg tacctggtct taaaggtgat
     3061 gatggcttgc agggtcagcc aggacttcct ggccctacag gagaaaaagg tagtaaagga
     3121 gagcctggcc ttccaggccc tcctggacca atggatccaa atcttctggg ctcaaaagga
     3181 gagaaggggg aacctggctt accaggtata cctggagttt cagggccaaa aggttatcag
     3241 ggtttgcctg gagacccagg gcaacctgga ctgagtggac aacctggatt accaggacca
     3301 ccaggtccca aaggtaaccc tggtctccct ggacagccag gtcttatagg acctcctgga
     3361 cttaaaggaa ccatcggtga tatgggtttt ccagggcctc agggtgtgga agggcctcct
     3421 ggaccttctg gagttcctgg acaacctggc tccccaggat tacctggaca gaaaggcgac
     3481 aaaggtgatc ctggtatttc aagcattggt cttccaggtc ttcctggtcc aaagggtgag
     3541 cctggtctgc ctggataccc agggaaccct ggtatcaaag gttctgtggg agatcctggt
     3601 ttgcccggat taccaggaac ccctggagca aaaggacaac caggccttcc tggattccca
     3661 ggaaccccag gccctcctgg accaaaaggt attagtggcc ctcctgggaa ccccggcctt
     3721 ccaggagaac ctggtcctgt aggtggtgga ggtcatcctg ggcaaccagg gcctccaggc
     3781 gaaaaaggca aacccggtca agatggtatt cctggaccag ctggacagaa gggtgaacca
     3841 ggtcaaccag gctttggaaa cccaggaccc cctggacttc caggactttc tggccaaaag
     3901 ggtgatggag gattacctgg gattccagga aatcctggcc ttccaggtcc aaagggcgaa
     3961 ccaggctttc acggtttccc tggtgtgcag ggtcccccag gccctcctgg ttctccgggt
     4021 ccagctctgg aaggacctaa aggcaaccct gggccccaag gtcctcctgg gagaccaggt
     4081 cctacaggtt ttcaaggtct accaggtcca gaaggtcctc caggtctccc tggaaatgga
     4141 ggtattaaag gagagaaggg aaatccaggc caacctgggc tacctggctt gcctggtttg
     4201 aaaggagatc aaggaccacc aggactccag ggtaatcctg gccggccggg tctcaatgga
     4261 atgaaaggag atcctggtct ccctggtgtt ccaggattcc caggcatgaa aggacccagt
     4321 ggagtacctg gatcagctgg ccctgagggg gaaccgggac ttattggtcc tccaggtcct
     4381 cctggattac ctggtccttc aggacagagt atcataatta aaggagatgc tggtcctcca
     4441 ggaatccctg gccagcctgg gctaaagggt ctaccaggac cccaaggacc tcaaggctta
     4501 ccaggtccaa ctggccctcc aggagatcct ggacgcaatg gactccctgg ctttgatggt
     4561 gcaggagggc gcaaaggaga cccaggtctg ccaggacagc caggtacccg tggtttggat
     4621 ggtccccctg gtccagatgg attgcaaggt cccccaggtc cccctggaac ctcctctgtt
     4681 gcacatggat ttcttattac acgccacagc cagacaacgg atgcaccaca atgcccacag
     4741 ggaacacttc aggtctatga aggcttttct ctcctgtatg tacaaggaaa taaaagagcc
     4801 cacggtcaag acttggggac ggctggcagc tgccttcgtc gctttagtac catgcctttc
     4861 atgttctgca acatcaataa tgtttgcaac tttgcttcaa gaaatgacta ttcttactgg
     4921 ctctctaccc cagagcccat gccaatgagc atgcaacccc taaagggcca gagcatccag
     4981 ccattcatta gtcgatgtgc agtatgtgaa gctccagctg tggtgatcgc agttcacagt
     5041 cagacgatcc agattcccca ttgtcctcag ggatgggatt ctctgtggat tggttattcc
     5101 ttcatgatgc atacaagtgc aggggcagaa ggctcaggtc aagccctagc ctcccctggt
     5161 tcctgcttgg aagagtttcg ttcagctccc ttcatcgaat gtcatgggag gggtacctgt
     5221 aactactatg ccaactccta cagcttttgg ctggcaactg tagatgtgtc agacatgttc
     5281 agtaaacctc agtcagaaac gctgaaagca ggagacttga ggacacgaat tagccgatgt
     5341 caagtgtgca tgaagaggac ataacatttt gaagaattcc ttttgtgttt taaaatgtga
     5401 tatatatata tataaaattc ctaggatgca gtgtctcatt gtccccaact ttactactgc
     5461 tgccgtcaat ggtgctacta tatatgatca agataacatg ctgactagta accatgaaga
     5521 ttcagatgta cctcagcaat gcgccagagc aaagtctcta ttatttttct actaaagaaa
     5581 taaggaagtg aatttacttt ttgggtccag aatgactttc tccaagaatt ataagatgaa
     5641 aattatatat tttgcccagt tactaaaatg gtacattaaa aattcaatta agagaagagt
     5701 cacattgagt aaaataaaag actgcagttt gtgggaagaa ttatttttca cggtgctact
     5761 aatcctgctg tatcccgggt ttttaatata aaggtgttaa gcttattttg ctttgtaagt
     5821 aaagaatgtg tatattgtga acagcctttt agctcaaaat gttgagtcat ttacatatga
     5881 catagcatga atcactcttt acagaaaatg taggaaaccc tagaatacag acagcaatat
     5941 tttatattca tgtttatcaa agtgagagga cttatattcc tacatcaagt tactactgag
     6001 agtaaattta ttttgagttt tatcccgtaa gttctgtttt gatttttttt aaaaaacaaa
     6061 cccttttagt cactttaatc agaattttaa atgttcatgt tacataccaa attataatat
     6121 ctaatggagc aatttgtctt ttgctatatt ctccaagatt atctcttaag accatatgcc
     6181 ccctgtttta atgtttctta catcttgttt ttactcattt ctgactggac aaagttcttc
     6241 caaacaattc tgagaaacaa aaacacacac gcagaattaa caattctttt ccctgtgctt
     6301 cttatgtaag aatcctcctg tggcctctgc ttgtacagaa ctgggaaaca acacttggtt
     6361 agtctctttt aagttacaaa aagccaattg atgtttctta ttctttttaa attttaaata
     6421 ttttgttata aatactcaca ggatacctta tttccctagc tatcatctcc tgacttaatg
     6481 ttttttaaac ccaccaatat aaatttaatt aaagatatat gttgtaagga t
//
