LOCUS       NM_000092               9984 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens collagen type IV alpha 4 chain (COL4A4), mRNA.
ACCESSION   NM_000092
VERSION     NM_000092.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9984)
  AUTHORS   Wang,D., Pan,M., Li,H., Li,M., Li,P., Xiong,F. and Xiao,H.
  TITLE     Four novel mutations identified in the COL4A3, COL4A4 and COL4A5
            genes in 10 families with Alport syndrome
  JOURNAL   BMC Med Genomics 17 (1), 181 (2024)
   PUBMED   38978054
  REMARK    GeneRIF: Four novel mutations identified in the COL4A3, COL4A4 and
            COL4A5 genes in 10 families with Alport syndrome.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9984)
  AUTHORS   Riedhammer,K.M., Simmendinger,H., Tasic,V., Putnik,J.,
            Abazi-Emini,N., Stajic,N., Berutti,R., Weidenbusch,M., Patzer,L.,
            Lungu,A., Milosevski-Lomic,G., Gunthner,R., Braunisch,M.C.,
            Comic,J. and Hoefele,J.
  TITLE     Is there a dominant-negative effect in individuals with
            heterozygous disease-causing variants in COL4A3/COL4A4?
  JOURNAL   Clin Genet 105 (4), 406-414 (2024)
   PUBMED   38214412
  REMARK    GeneRIF: Is there a dominant-negative effect in individuals with
            heterozygous disease-causing variants in COL4A3/COL4A4?
REFERENCE   3  (bases 1 to 9984)
  AUTHORS   Xiao,T., Zhang,J., Liu,L. and Zhang,B.
  TITLE     Genetic diagnosis of Alport syndrome in 16 Chinese families
  JOURNAL   Mol Genet Genomic Med 12 (3), e2406 (2024)
   PUBMED   38433557
  REMARK    GeneRIF: Genetic diagnosis of Alport syndrome in 16 Chinese
            families.
REFERENCE   4  (bases 1 to 9984)
  AUTHORS   Zheng,T., Zheng,Z., Zhou,H., Guo,Y. and Li,S.
  TITLE     The multifaceted roles of COL4A4 in lung adenocarcinoma: An
            integrated bioinformatics and experimental study
  JOURNAL   Comput Biol Med 170, 107896 (2024)
   PUBMED   38217972
  REMARK    GeneRIF: The multifaceted roles of COL4A4 in lung adenocarcinoma:
            An integrated bioinformatics and experimental study.
REFERENCE   5  (bases 1 to 9984)
  AUTHORS   Levy,M., Bazak,L., Lev-El,N., Greenberg,R., Kropach,N.,
            Basel-Salmon,L. and Maya,I.
  TITLE     Potential Founder Variants in COL4A4 Identified in Bukharian Jews
            Linked to Autosomal Dominant and Autosomal Recessive Alport
            Syndrome
  JOURNAL   Genes (Basel) 14 (10), 1854 (2023)
   PUBMED   37895203
  REMARK    GeneRIF: Potential Founder Variants in COL4A4 Identified in
            Bukharian Jews Linked to Autosomal Dominant and Autosomal Recessive
            Alport Syndrome.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 9984)
  AUTHORS   Kashtan,C.E.
  TITLE     Alport Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301386
REFERENCE   7  (bases 1 to 9984)
  AUTHORS   Kamagata,Y., Mattei,M.G. and Ninomiya,Y.
  TITLE     Isolation and sequencing of cDNAs and genomic DNAs encoding the
            alpha 4 chain of basement membrane collagen type IV and assignment
            of the gene to the distal long arm of human chromosome 2
  JOURNAL   J Biol Chem 267 (33), 23753-23758 (1992)
   PUBMED   1429714
REFERENCE   8  (bases 1 to 9984)
  AUTHORS   Mariyama,M., Zheng,K., Yang-Feng,T.L. and Reeders,S.T.
  TITLE     Colocalization of the genes for the alpha 3(IV) and alpha 4(IV)
            chains of type IV collagen to chromosome 2 bands q35-q37
  JOURNAL   Genomics 13 (3), 809-813 (1992)
   PUBMED   1639407
REFERENCE   9  (bases 1 to 9984)
  AUTHORS   Gupta,S., Batchu,R.B. and Datta,K.
  TITLE     Purification, partial characterization of rat kidney hyaluronic
            acid binding protein and its localization on the cell surface
  JOURNAL   Eur J Cell Biol 56 (1), 58-67 (1991)
   PUBMED   1724753
REFERENCE   10 (bases 1 to 9984)
  AUTHORS   Sanes,J.R., Engvall,E., Butkowski,R. and Hunter,D.D.
  TITLE     Molecular heterogeneity of basal laminae: isoforms of laminin and
            collagen IV at the neuromuscular junction and elsewhere
  JOURNAL   J Cell Biol 111 (4), 1685-1699 (1990)
   PUBMED   2211832
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC079235.7, X81053.1,
            BX094848.1 and AC073149.5.
            
            On Aug 31, 2019 this sequence version replaced NM_000092.4.
            
            Summary: This gene encodes one of the six subunits of type IV
            collagen, the major structural component of basement membranes.
            This particular collagen IV subunit, however, is only found in a
            subset of basement membranes. Like the other members of the type IV
            collagen gene family, this gene is organized in a head-to-head
            conformation with another type IV collagen gene so that each gene
            pair shares a common promoter. Mutations in this gene are
            associated with type II autosomal recessive Alport syndrome
            (hereditary glomerulonephropathy) and with familial benign
            hematuria (thin basement membrane disease). Two transcripts,
            differing only in their transcription start sites, have been
            identified for this gene and, as is common for collagen genes,
            multiple polyadenylation sites are found in the 3' UTR. [provided
            by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X81053.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000396625.5/ ENSP00000379866.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-199               AC079235.7         135923-136121       c
            200-1394            X81053.1           108-1302
            1395-1399           AC079235.7         71536-71540         c
            1400-2180           X81053.1           1308-2088
            2181-2287           BX094848.1         44-150
            2288-3310           X81053.1           2196-3218
            3311-3450           AC079235.7         22893-23032         c
            3451-4278           X81053.1           3359-4186
            4279-4381           AC073149.5         97503-97605         c
            4382-4506           X81053.1           4290-4414
            4507-4516           AC073149.5         91649-91658         c
            4517-5373           X81053.1           4425-5281
            5374-9984           AC073149.5         72315-76925         c
FEATURES             Location/Qualifiers
     source          1..9984
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q36.3"
     gene            1..9984
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /note="collagen type IV alpha 4 chain"
                     /db_xref="GeneID:1286"
                     /db_xref="HGNC:HGNC:2206"
                     /db_xref="MIM:120131"
     exon            1..199
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            200..371
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     CDS             301..5373
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /note="Collagen IV, alpha-4 polypeptide; collagen of
                     basement membrane, alpha-4 chain; collagen, type IV, alpha
                     4; collagen alpha-4(IV) chain"
                     /codon_start=1
                     /product="collagen alpha-4(IV) chain precursor"
                     /protein_id="NP_000083.3"
                     /db_xref="CCDS:CCDS42828.1"
                     /db_xref="GeneID:1286"
                     /db_xref="HGNC:HGNC:2206"
                     /db_xref="MIM:120131"
                     /translation="MWSLHIVLMRCSFRLTKSLATGPWSLILILFSVQYVYGSGKKYI
                     GPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGA
                     AGDKGDKGPTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPG
                     GPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGL
                     VGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFCLYKGEKGIKG
                     IPGMVGLPGPPGRKGESGIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDP
                     GLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGL
                     LGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQ
                     GLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGPKGEKGNEGLCACEPGPMGPPGPPGLP
                     GRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKG
                     HKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKA
                     GPVGPPGLGFPGPPGERGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFD
                     GPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSP
                     VGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAG
                     IPGFLGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQ
                     PGDVGPPGPAGMKGLPGLPGRPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPRGLPGFP
                     GFPGERGKPGAEGCPGAKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKG
                     TPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFHRGEPGEKGQPGPPGPPG
                     PPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKG
                     EPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGE
                     MGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVG
                     IPGLKGERGDPGSPGISPPGPRGKKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGD
                     QGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGK
                     DGQKGPVGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRI
                     PGLPGAPGMRGPEGAMGLPGMRGPSGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTG
                     EDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLL
                     YLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMM
                     PLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMHTGAGDQ
                     GGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPD
                     TLKESQAQRQKISRCQVCVKYS"
     exon            372..414
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            415..492
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            493..627
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            628..672
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            673..789
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            790..858
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            859..894
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            895..957
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            958..993
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            994..1035
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            1036..1116
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            1117..1170
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            1171..1230
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            1231..1275
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            1276..1329
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            1330..1399
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            1400..1504
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            1505..1669
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            1670..1759
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            1760..1923
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            1924..1996
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            1997..2103
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            2104..2287
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            2288..2356
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            2357..2464
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            2465..2683
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            2684..2845
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            2846..3016
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            3017..3160
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            3161..3268
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            3269..3450
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            3451..3514
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            3515..3589
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            3590..3697
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            3698..3805
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            3806..3877
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            3878..4006
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            4007..4117
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            4118..4273
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            4274..4381
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            4382..4390
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            4391..4516
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            4517..4633
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            4634..4822
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            4823..5109
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     exon            5110..9984
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9957..9962
                     /regulatory_class="polyA_signal_sequence"
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /note="hexamer: TATAAA"
     polyA_site      9984
                     /gene="COL4A4"
                     /gene_synonym="ATS2; BFH; BFH1; CA44"
                     /note="major polyA site"
ORIGIN      
        1 actcgggagg ccccgcttgt tccccgcgcc cgcggcggtc cgcgttcccc gcctccagcc
       61 cgcctccaac ttgcggccgc cgggcttggc tgcgagagcg ccgcgaagag gcagcggggc
      121 gcgggtggat tggggctgga ggtgcgcgtc ccgtggggtg gcaaggcggc agctcctggc
      181 gctgcgggcg tccccacagg aacagacttt gacccagaac acagaacctc acttgtcaac
      241 aagaaccttc tggaagagaa gactggcaga atatttttta agtactaaga cttgcctgcg
      301 atgtggtctc tgcacatagt actaatgagg tgctccttca gattgaccaa gtccttggcc
      361 acaggtccct ggtcacttat actcattctc ttttctgtac aatatgtata tgggagtgga
      421 aagaaataca ttggtccttg tggaggaaga gattgctctg tttgccactg tgttcctgaa
      481 aaggggtctc ggggtccacc aggaccacca gggccacagg gtccaattgg acccctggga
      541 gccccaggac ccattgggct ttcaggagag aaaggaatga gaggggaccg cggccctcct
      601 ggagcagcag gggacaaagg agataagggt ccaactggtg ttcctggatt tccaggttta
      661 gatggcatac ctgggcaccc agggcctcct ggacccagag gcaaacctgg tatgagtggc
      721 cacaatggct caagaggtga cccagggttt ccaggaggaa gaggagctct tggcccagga
      781 ggccccctag gccatcctgg ggaaaaggga gaaaaaggaa attcagtgtt cattttaggt
      841 gccgttaaag gtattcaggg agacagaggg gacccaggac tgcctggctt accaggatct
      901 tggggtgcag gaggaccggc aggtcccaca ggatatcctg gagagccagg gttagtggga
      961 cctccgggcc aaccagggcg tccaggtttg aagggaaatc ccggtgtggg agtaaagggg
     1021 caaatgggag acccgggtga ggttggtcag caaggttctc ctggacccac cctgttggta
     1081 gagccacctg acttttgtct ctataaagga gaaaagggta taaaaggaat tcctggaatg
     1141 gttggactgc caggaccacc aggacgcaag ggagaatctg gtattggggc aaaaggagaa
     1201 aaaggtattc ctggatttcc agggcctcgg ggggatcctg gttcctatgg atctccaggt
     1261 tttccaggat taaagggaga actaggactg gttggagatc ctgggctatt tggattaatt
     1321 ggcccaaagg gggatcctgg aaatcgaggg cacccaggac caccaggtgt tttggtgact
     1381 ccacctcttc cactcaaagg cccaccaggg gacccagggt tccctggccg ctatggagaa
     1441 acaggggatg ttggaccacc tggtccccca ggtctcttgg gcagaccagg ggaagcctgt
     1501 gcaggcatga taggaccccc tgggccacaa ggatttcctg gtcttcctgg gcttccagga
     1561 gaagctggta ttcctgggag acctgattct gctccaggaa aaccagggaa gccaggatca
     1621 cctggcttgc ctggagcacc aggcctgcag ggcctcccag gatcaagtgt gatatactgt
     1681 agtgttggga accccggacc acaaggaata aaaggcaaag ttggtccccc aggaggaaga
     1741 ggcccaaaag gagaaaaagg aaatgaagga ctctgtgcct gtgagcctgg acccatgggc
     1801 ccccctggcc ctccaggact tcctgggagg caggggagta agggagactt ggggctccct
     1861 ggctggcttg gaacaaaagg tgacccagga cctcctggtg ctgaaggacc tccagggcta
     1921 ccaggaaagc atggtgcctc tggaccacct ggcaacaaag gggcgaaggg tgacatggtt
     1981 gtatcaagag ttaaagggca caaaggagaa agaggtcctg atgggccccc aggatttcca
     2041 gggcagccag gatcacatgg tcgggatgga catgctggag aaaaagggga tccaggacct
     2101 ccaggggatc atgaagatgc gaccccaggt ggtaaaggat ttcctggacc tctgggcccc
     2161 ccaggcaaag caggacctgt ggggccccca ggactgggat ttcctggtcc accaggagag
     2221 cgaggccacc caggagttcc aggccaccca ggtgtgaggg gccctgatgg cttgaagggt
     2281 cagaaaggtg acacaatttc ttgcaacgta acctaccctg ggaggcatgg ccctccaggt
     2341 tttgatggac ctccaggtcc gaagggattt ccaggtcccc aaggtgcccc tgggctgagt
     2401 ggttcagatg ggcataaagg cagacctggc acaccaggaa cagcggaaat accaggtcca
     2461 cctggttttc gtggtgacat gggagatccg ggttttggag gtgaaaaggg gtcctcccct
     2521 gttgggcccc caggccctcc cggctcacca ggagtgaatg gtcagaaagg aatcccggga
     2581 gaccctgcat ttggtcacct gggacccccg ggaaagaggg gtctttcagg agtgccaggg
     2641 ataaaaggac ccagaggtga tccgggatgt ccaggggctg aagggccagc tggcattcct
     2701 ggattcctag gtctcaaagg tcccaaaggc agagagggac atgctgggtt tccaggtgtc
     2761 ccaggtccac ctggccattc ctgtgaaaga ggtgctccag ggataccagg gcaaccggga
     2821 ctccctgggt atccaggtag cccaggtgct ccaggtggga aaggacagcc gggagatgtg
     2881 gggcctcccg ggccagctgg aatgaaaggc ctccccggac tcccaggacg gcctggggca
     2941 catggtcccc caggcctccc aggaatccca ggtccctttg gagatgatgg gctacctggt
     3001 cctccaggtc caaagggacc ccgggggctg cctggtttcc caggttttcc cggagaaaga
     3061 ggaaagcctg gtgcagaggg atgtcctggc gcaaagggag aacctggaga gaagggcatg
     3121 tctggccttc ctggagaccg gggactgaga ggggccaaag gagccatagg acctcccgga
     3181 gatgaaggag aaatggctat catttcacaa aagggaacac ctggggaacc tggacctcct
     3241 ggagatgatg gattcccagg agaaagaggt gataaaggaa ctcccgggat gcaagggaga
     3301 agaggagagc cgggaagata cggaccacct ggatttcaca gaggggaacc tggtgagaaa
     3361 ggtcagccag ggcctcctgg acccccaggc cctccaggct caactggtct aagagggttc
     3421 attggttttc caggacttcc aggtgaccag ggtgagccag gttctccagg tccccctgga
     3481 ttttcaggaa ttgatggagc aagaggacct aaaggaaaca aaggtgaccc tgccagtcac
     3541 tttggtccac ctggtccaaa gggtgagcca ggtagccctg gatgtccagg gcattttgga
     3601 gcatccggag agcagggctt gcctggtatt caagggccca gaggatcacc tggaaggcca
     3661 gggccacctg gctcctctgg accaccaggg tgcccaggtg atcacgggat gcctgggctg
     3721 aggggacagc caggagaaat gggagaccct gggccaagag gcctccaggg ggatccaggg
     3781 ataccaggtc ctccgggaat aaaaggtccc tccggatcac ctggcctgaa cggcttgcat
     3841 ggattgaaag gtcagaaagg aactaaaggt gcttcaggtt tgcatgatgt ggggccacct
     3901 ggtccagtgg gaatacctgg gctaaaaggg gagagaggag accctgggag cccaggaatc
     3961 tctcctccag gtcctcgtgg aaagaaaggt cccccaggac ccccagggag ttcaggacca
     4021 cctggtcctg caggtgccac aggaagagct cctaaggaca ttcctgaccc gggtccacct
     4081 ggagatcagg gacctcctgg tcctgatggc ccaagaggag cacctgggcc tccaggcctc
     4141 cctgggagtg ttgaccttct gagaggggag ccaggtgact gtggtctacc agggccacca
     4201 ggtccccctg gcccaccagg ccctccagga tacaaaggct ttccaggatg tgatggaaaa
     4261 gatggccaga aaggaccagt gggattcccg ggaccgcagg gaccacatgg atttcctggg
     4321 ccacctggag agaagggttt acctggacct ccagggagaa aagggcccac tggtcttccg
     4381 ggtcccagag gtgaaccggg gccacctgca gatgtggatg actgtccccg aatcccaggc
     4441 cttcctgggg cgccaggcat gagaggacca gaaggagcca tggggctccc tggaatgaga
     4501 ggcccctcag gaccagggtg caaaggagag cctgggctgg atggcaggag gggtgtggat
     4561 ggcgtccctg ggtctcctgg gcctcccgga cgtaaaggtg acacaggaga agacggctac
     4621 cctggaggac cagggcctcc tggtcccatt ggggatcctg ggcccaaagg gtttggccct
     4681 ggatacctcg gtggcttcct cctggttctc cacagtcaga cggaccagga gcccacctgc
     4741 cccctgggca tgcccaggct ctggactggg tatagtctgt tatacctgga agggcaagag
     4801 aaagctcaca atcaagacct tggtctggca gggtcttgcc ttcccgtatt tagcacgctg
     4861 ccctttgcct actgcaacat ccaccaggtg tgccactatg cccagagaaa cgacagatcc
     4921 tactggctgg ccagcgctgc gcccctcccc atgatgccac tctctgaaga ggcgatccgc
     4981 ccctatgtca gccgctgtgc ggtatgcgag gccccggccc aggcggtggc ggtgcacagc
     5041 caggaccagt ccatcccccc atgtccgcag acctggagga gcctctggat cgggtattca
     5101 ttcctgatgc acacaggagc tggggaccaa ggaggagggc aggcccttat gtcacctggc
     5161 agctgcctgg aagatttcag agcagcacca ttccttgaat gccagggccg gcagggaact
     5221 tgccactttt tcgcaaataa gtatagcttc tggctcacaa cggtgaaagc agacttgcag
     5281 ttttcctctg ctccagcacc agacacctta aaagaaagcc aggcccaacg ccagaaaatc
     5341 agccggtgcc aggtctgcgt gaagtatagc tagagaatgc gaaattcacc aacacgtggc
     5401 caagagaaac ttcctagggg gctaagactt cctagactgt gctaagagat gtcatggtgc
     5461 tcattttgga ctccctttca gtggttcctt ctgttttgtc gtggttattc ccagagtcct
     5521 ctggttcctt acacattaag caaatgctgc acagatggat ttgtttggac ctcccaatct
     5581 agggagccta gatactctta ttttactgag gatgatcgaa gaactggctt tacttaaaaa
     5641 tatgctaatt ctcagaaagg caagtagatg ataaaggcca gattacaaat tacattactg
     5701 aaaacttcat tcttgggtta acagtatctc aaacaattga agtcaattac tctataatac
     5761 agtgggcttc tggatggatt ttataggaaa aaataaacag gtcaatgaat gaaactagaa
     5821 agcagagatt ttcaacattt caaaatgatt tcctctgtaa tctatttttc catatacttt
     5881 aaataatggt aaaaccatga cgcaaagaga gatttttttt taaagaagaa aaaaaaaaat
     5941 tcacactgcc agcgttaaca gttcctttca aaggagaatg aatcatgatg gcaggaaggc
     6001 ccacagtcgc cgtattccag agatgcgaca ttagcataaa cacatcacag atgaatataa
     6061 aacattatgt tctcttctgc atttttcaga gaatagaaat gcctactttg gcaacccttt
     6121 tgaaaagtag caattatgga aaaaaaatat tcaataagag attaggagcc taaaagctat
     6181 tagtgaatat taaggtagtt attcacaaaa attgactccc cattgcagtg aacttccaga
     6241 cagactgctt ttccccagtc ggggtccggc gtgtcacagg tgcgtgcgtg ctaatgggac
     6301 tgacgctaca tggggctcac tcaggcaggc acgcgcttca tacaaagcat ctcactcccc
     6361 tccccaggag agcctgcaca gcttcttgca ctcacaacgg acactttgct ccacacacat
     6421 aatggcagct cacacaggga cgtgacagag ctatcattat gcacttggga gaaaattaag
     6481 ggccgattta attaaactta ggtaagaaga ttcatttaag tcagggttac cccatcagga
     6541 ggacatggca ctatctttaa acgaaacaaa gacaatttat aatttgaatt ttatgctccc
     6601 gtggttggct gttacaggag catcccattt tgccaatttt aaagacattc ttatatttca
     6661 tatcagtctt gtaccaaggc aacagtttga catttggcat tagtattttc taaaaaagtt
     6721 tagaatgtgt gtcaatttat aatgattatt tttttctgta aagcaaaaga tccctttttc
     6781 tgttttgcta ggaatttggt gatctaatcc taaatttaaa agatttgttg gaaaaaattt
     6841 ttaggaaact caccttcctc atctaaaaga aaaaggcatt ttagagaaaa ctaaagaaat
     6901 ttctcatgag cgtgacactc attttagtgc tttgtttccg tgcacttaaa aataattgag
     6961 aagaaaaact caattaaaat tttgtttata agaaatgttt tccttgccaa accttgattt
     7021 gtaatgagct cttatatgca gaacacattt caaatgagtt ttgctatggg ctgcccccag
     7081 ggtggcaatt tattttaaga gtattttctg gtaaaaagaa aaatgtgtat tttaagatga
     7141 aatattttct tgatgtagca gaatatttcc tagttcattt gacccatttg atatttttta
     7201 aaccatgctc tggcatgttg aatatttttg tgcacctaaa acttaagcca atttcaatct
     7261 tatttgtgat tacctttctc cttcccaaaa agctttatct attaccaaaa gtcaaccctc
     7321 ctaaaagttc aacctgttca tcttgaactt ggcctgagaa cattttctgg gaagaggtaa
     7381 gggtgacaaa tggaacatca gaaacgtatc ttgcttgcta attattttaa acactttaat
     7441 gttggtatta gaatattatc ttcataagtt aataaataag taaaaaaaaa aaacccacac
     7501 aaagacaggc taaattatgt agacagttta ctccacagtg agcttgggaa ggtatcagac
     7561 tgttcaaaat gttagtatta caagagctgt ctctaaatgc agcattaaca ctgccctcta
     7621 attttacaaa agtattttaa aggatcaaaa gggcggaaaa ataacttctt ggaatagaag
     7681 atcctgttac cttaaaccat tattttcttc acctggttat atatcaatgt gccttcaatt
     7741 accctttctc tgtggaggtg atgaacaaag cttttaagtt gttctcctaa cccattattg
     7801 gagatgatcc ttatcaaacc cttctctccc tgcctcccca ccaaccttgt agactggaac
     7861 gtgcctagat tgccctaatg ggaactgttc tgttctgtgc ccgtgctgga aatctgggat
     7921 gcttccttct gggttctgat tctcagtgag tggagcccat tcatccaggc aggtttccat
     7981 gctatgagtg gccctttcgt tctgctctca cactccccac attgaaagct gagtcatcgc
     8041 tgctttggat ggctacctcc tctttagccc cgtgcccgtc tcacacagcc tttgttttcc
     8101 acctccaccc ttgcagtagg catctcccag ttggtgcctt agtttcaaat cccagttgcc
     8161 atctaagatc cacccaaact tgattttgtc accctctgga ttagaatcct tctgtgactc
     8221 cgctgcccat gaagtccaaa tgacttccta tggggtgaaa ggtccagtgg ggactgttcg
     8281 agggttccct ctaggagtat ttcctgtcat gatgaactgt gtgcagctct gcagacactc
     8341 ccagcacctc ttcgactttg ctcctgcttc accagtgatg tcctccctgg ggtcagttta
     8401 ccctgcgtgg gaggtgaatt ccctggggga aacttaggct cccttctgct gctcttgtgc
     8461 agtgcacggg gctgtcctca ttttctccgt tccccattgc ttctttaatg tctcagtgca
     8521 cttccttcat tccattgcca aaatgcaagc cccttgaggg cagggaccat gcttgactcg
     8581 tttttgtatg cctaatgcct agcacagagt tgaacacatt actgccaatt catttttttc
     8641 aatgacaatt ccataaacat cattaggcat taggtgttca gctctgcata taaggtcttc
     8701 atcagcaaga cggttacaag tataaaccat ttcctatttc agaatatttt aatattcaac
     8761 atatttgtat acccacattc tcttgttaca attattgcca gaaagccaat tgataagctt
     8821 tcaaaatggg cttatcacac ccctccaaaa atagattttt gttttttcta taaacagaaa
     8881 tttcagaaat gagatttgag agatccgact gagaagaccc ttcagtaaaa tgtgacatga
     8941 cttaccttat gctgtaatct gtattccctc aggatttaag agcttcctag cataaaatct
     9001 ttgctaaacg tttgtgtaca tgttattggt gctccgaaaa atagtcaatg gaatggtgca
     9061 ctacaattgg gctttatcat cttattttga acctcgtgta gattacagta aagcaaacca
     9121 ttcagtgtgt tccaggaaat tatattagat ctgtgtttct atcagctcac tggaataatc
     9181 tgataattgt tacttttact tggtatggct gcaagaatag tggaaagaag aggacttgag
     9241 agttggtcga acccaggttt gaaatctggc ttagtcattt ttcagctgtt atcctgggtg
     9301 agttttgcca actttcttag cttcatttcc ctcattaata cggtgggaca taacagctac
     9361 tcttgcattg aaaattaagt tagattacct gtctagcata cccatcatca tacacttact
     9421 atggtcacat tttgtattta aaataaacta atacgaaaaa tatttctttt tttttacaca
     9481 gaattatgat tctcacaggg tatataaatt actgattaga attatttata tgtggccaat
     9541 tcttaatgtc attgggaatg ctgtttcatt tcaatcctca aagttactgt agcacagaaa
     9601 atatcacaat ttcctgcagg gacattatca gtaagttctg cagggaacaa acaattgaca
     9661 ttaaaaatca gtactctgca attgtcactg ttatcatctg ctagaaactc tgcataatgc
     9721 attttaaacc accaaggctg gctgccacat ccatgtgaaa tgcttgaatt ttatggtgct
     9781 taaatattta atgattcatg ggaaaaatgt gaaatgtgtc taataaattg catccctttc
     9841 tctaacctct ggttgtaaag ttaaagactt tcagcatgta acttttgcaa gatgcctggt
     9901 ctgccattgg cacttaaata tttgttgtat tacgatttat aatatggttc attatatata
     9961 aaattctgtg atgacttcca aaaa
//
