LOCUS       NM_000090               5490 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens collagen type III alpha 1 chain (COL3A1), mRNA.
ACCESSION   NM_000090
VERSION     NM_000090.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5490)
  AUTHORS   Chen,W.K., Zhang,H.J., Liu,J., Dai,Z. and Zhan,X.L.
  TITLE     COL3A1 is a potential diagnostic biomarker for synovial
            chondromatosis and affects the cell cycle and migration of
            chondrocytes
  JOURNAL   Int Immunopharmacol 127, 111416 (2024)
   PUBMED   38145599
  REMARK    GeneRIF: COL3A1 is a potential diagnostic biomarker for synovial
            chondromatosis and affects the cell cycle and migration of
            chondrocytes.
REFERENCE   2  (bases 1 to 5490)
  AUTHORS   Zhang,M., Liang,Y. and Song,P.
  TITLE     COL3A1-positive endothelial cells influence LUAD prognosis and
            regulate LUAD carcinogenesis by NCL-PI3K-AKT axis
  JOURNAL   J Gene Med 26 (1), e3573 (2024)
   PUBMED   37547956
  REMARK    GeneRIF: COL3A1-positive endothelial cells influence LUAD prognosis
            and regulate LUAD carcinogenesis by NCL-PI3K-AKT axis.
REFERENCE   3  (bases 1 to 5490)
  AUTHORS   Heo,W.Y., Jang,S.Y., Park,T.K., Ki,C.S., Kim,J.W., Kim,D.K. and
            Jang,J.H.
  TITLE     Identification of two novel COL3A1 variants in patients with
            vascular Ehlers-Danlos syndrome
  JOURNAL   Mol Genet Genomic Med 11 (9), e2240 (2023)
   PUBMED   37461200
  REMARK    GeneRIF: Identification of two novel COL3A1 variants in patients
            with vascular Ehlers-Danlos syndrome.
REFERENCE   4  (bases 1 to 5490)
  AUTHORS   Li,H., Cao,L. and Li,H.
  TITLE     COL3A1, CXCL8, VCAN, THBS2, and COL1A2 are correlated with the
            onset of biliary atresia
  JOURNAL   Medicine (Baltimore) 102 (11), e33299 (2023)
   PUBMED   36930067
  REMARK    GeneRIF: COL3A1, CXCL8, VCAN, THBS2, and COL1A2 are correlated with
            the onset of biliary atresia.
REFERENCE   5  (bases 1 to 5490)
  AUTHORS   Byers,P.H.
  TITLE     Vascular Ehlers-Danlos Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301667
REFERENCE   6  (bases 1 to 5490)
  AUTHORS   Longoni,M., Pober,B.R. and High,F.A.
  TITLE     Congenital Diaphragmatic Hernia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301533
REFERENCE   7  (bases 1 to 5490)
  AUTHORS   Milewicz,D.M. and Cecchi,A.C.
  TITLE     Heritable Thoracic Aortic Disease Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301299
REFERENCE   8  (bases 1 to 5490)
  AUTHORS   Richards,A.J., Ward,P.N., Narcisi,P., Nicholls,A.C., Lloyd,J.C. and
            Pope,F.M.
  TITLE     A single base mutation in the gene for type III collagen (COL3A1)
            converts glycine 847 to glutamic acid in a family with
            Ehlers-Danlos syndrome type IV. An unaffected family member is
            mosaic for the mutation
  JOURNAL   Hum Genet 89 (4), 414-418 (1992)
   PUBMED   1352273
REFERENCE   9  (bases 1 to 5490)
  AUTHORS   Seyer,J.M. and Kang,A.H.
  TITLE     Covalent structure of collagen: amino acid sequence of five
            consecutive CNBr peptides from type III collagen of human liver
  JOURNAL   Biochemistry 17 (16), 3404-3411 (1978)
   PUBMED   687591
REFERENCE   10 (bases 1 to 5490)
  AUTHORS   Seyer,J.M. and Kang,A.H.
  TITLE     Covalent structure of collagen: amino acid sequence of cyanogen
            bromide peptides from the amino-terminal segment of type III
            collagen of human liver
  JOURNAL   Biochemistry 16 (6), 1158-1164 (1977)
   PUBMED   557335
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC066694.7.
            
            On Dec 4, 2019 this sequence version replaced NM_000090.3.
            
            Summary: This gene encodes the pro-alpha1 chains of type III
            collagen, a fibrillar collagen that is found in extensible
            connective tissues such as skin, lung, uterus, intestine and the
            vascular system, frequently in association with type I collagen.
            Mutations in this gene are associated with Ehlers-Danlos syndrome
            type IV, and with aortic and arterial aneurysms. [provided by R.
            Dalgleish, Feb 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X14420.1, SRR11853561.17153.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000304636.9/ ENSP00000304408.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-196               AC066694.7         79333-79528
            197-399             AC066694.7         89720-89922
            400-450             AC066694.7         90157-90207
            451-564             AC066694.7         90625-90738
            565-645             AC066694.7         92019-92099
            646-699             AC066694.7         93041-93094
            700-753             AC066694.7         93550-93603
            754-807             AC066694.7         94356-94409
            808-861             AC066694.7         95056-95109
            862-915             AC066694.7         95267-95320
            916-969             AC066694.7         95964-96017
            970-1014            AC066694.7         96447-96491
            1015-1068           AC066694.7         96629-96682
            1069-1113           AC066694.7         97144-97188
            1114-1167           AC066694.7         97847-97900
            1168-1266           AC066694.7         98321-98419
            1267-1311           AC066694.7         98998-99042
            1312-1410           AC066694.7         99194-99292
            1411-1464           AC066694.7         99501-99554
            1465-1572           AC066694.7         99684-99791
            1573-1626           AC066694.7         100006-100059
            1627-1725           AC066694.7         100652-100750
            1726-1779           AC066694.7         101085-101138
            1780-1878           AC066694.7         101358-101456
            1879-1932           AC066694.7         102125-102178
            1933-1986           AC066694.7         102296-102349
            1987-2040           AC066694.7         102660-102713
            2041-2094           AC066694.7         103226-103279
            2095-2139           AC066694.7         103634-103678
            2140-2238           AC066694.7         104245-104343
            2239-2346           AC066694.7         104430-104537
            2347-2400           AC066694.7         104802-104855
            2401-2454           AC066694.7         106357-106410
            2455-2508           AC066694.7         106496-106549
            2509-2562           AC066694.7         107258-107311
            2563-2670           AC066694.7         107915-108022
            2671-2724           AC066694.7         108371-108424
            2725-2778           AC066694.7         108694-108747
            2779-2940           AC066694.7         108942-109103
            2941-3048           AC066694.7         109217-109324
            3049-3156           AC066694.7         110310-110417
            3157-3210           AC066694.7         111166-111219
            3211-3318           AC066694.7         111305-111412
            3319-3372           AC066694.7         111897-111950
            3373-3480           AC066694.7         112460-112567
            3481-3534           AC066694.7         112845-112898
            3535-3642           AC066694.7         112995-113102
            3643-3940           AC066694.7         113884-114181
            3941-4128           AC066694.7         115138-115325
            4129-4371           AC066694.7         115608-115850
            4372-5490           AC066694.7         116588-117706
FEATURES             Location/Qualifiers
     source          1..5490
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q32.2"
     gene            1..5490
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /note="collagen type III alpha 1 chain"
                     /db_xref="GeneID:1281"
                     /db_xref="HGNC:HGNC:2201"
                     /db_xref="MIM:120180"
     exon            1..196
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     CDS             118..4518
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /note="Ehlers-Danlos syndrome type IV, autosomal dominant;
                     collagen, type III, alpha 1; collagen, fetal; collagen
                     alpha-1(III) chain; alpha-1 type III collagen; alpha1
                     (III) collagen"
                     /codon_start=1
                     /product="collagen alpha-1(III) chain preproprotein"
                     /protein_id="NP_000081.2"
                     /db_xref="CCDS:CCDS2297.1"
                     /db_xref="GeneID:1281"
                     /db_xref="HGNC:HGNC:2201"
                     /db_xref="MIM:120180"
                     /translation="MMSFVQKGSWLLLALLHPTIILAQQEAVEGGCSHLGQSYADRDV
                     WKPEPCQICVCDSGSVLCDDIICDDQELDCPNPEIPFGECCAVCPQPPTAPTRPPNGQ
                     GPQGPKGDPGPPGIPGRNGDPGIPGQPGSPGSPGPPGICESCPTGPQNYSPQYDSYDV
                     KSGVAVGGLAGYPGPAGPPGPPGPPGTSGHPGSPGSPGYQGPPGEPGQAGPSGPPGPP
                     GAIGPSGPAGKDGESGRPGRPGERGLPGPPGIKGPAGIPGFPGMKGHRGFDGRNGEKG
                     ETGAPGLKGENGLPGENGAPGPMGPRGAPGERGRPGLPGAAGARGNDGARGSDGQPGP
                     PGPPGTAGFPGSPGAKGEVGPAGSPGSNGAPGQRGEPGPQGHAGAQGPPGPPGINGSP
                     GGKGEMGPAGIPGAPGLMGARGPPGPAGANGAPGLRGGAGEPGKNGAKGEPGPRGERG
                     EAGIPGVPGAKGEDGKDGSPGEPGANGLPGAAGERGAPGFRGPAGPNGIPGEKGPAGE
                     RGAPGPAGPRGAAGEPGRDGVPGGPGMRGMPGSPGGPGSDGKPGPPGSQGESGRPGPP
                     GPSGPRGQPGVMGFPGPKGNDGAPGKNGERGGPGGPGPQGPPGKNGETGPQGPPGPTG
                     PGGDKGDTGPPGPQGLQGLPGTGGPPGENGKPGEPGPKGDAGAPGAPGGKGDAGAPGE
                     RGPPGLAGAPGLRGGAGPPGPEGGKGAAGPPGPPGAAGTPGLQGMPGERGGLGSPGPK
                     GDKGEPGGPGADGVPGKDGPRGPTGPIGPPGPAGQPGDKGEGGAPGLPGIAGPRGSPG
                     ERGETGPPGPAGFPGAPGQNGEPGGKGERGAPGEKGEGGPPGVAGPPGGSGPAGPPGP
                     QGVKGERGSPGGPGAAGFPGARGLPGPPGSNGNPGPPGPSGSPGKDGPPGPAGNTGAP
                     GSPGVSGPKGDAGQPGEKGSPGAQGPPGAPGPLGIAGITGARGLAGPPGMPGPRGSPG
                     PQGVKGESGKPGANGLSGERGPPGPQGLPGLAGTAGEPGRDGNPGSDGLPGRDGSPGG
                     KGDRGENGSPGAPGAPGHPGPPGPVGPAGKSGDRGESGPAGPAGAPGPAGSRGAPGPQ
                     GPRGDKGETGERGAAGIKGHRGFPGNPGAPGSPGPAGQQGAIGSPGPAGPRGPVGPSG
                     PPGKDGTSGHPGPIGPPGPRGNRGERGSEGSPGHPGQPGPPGPPGAPGPCCGGVGAAA
                     IAGIGGEKAGGFAPYYGDEPMDFKINTDEIMTSLKSVNGQIESLISPDGSRKNPARNC
                     RDLKFCHPELKSGEYWVDPNQGCKLDAIKVFCNMETGETCISANPLNVPRKHWWTDSS
                     AEKKHVWFGESMDGGFQFSYGNPELPEDVLDVHLAFLRLLSSRASQNITYHCKNSIAY
                     MDQASGNVKKALKLMGSNEGEFKAEGNSKFTYTVLEDGCTKHTGEWSKTVFEYRTRKA
                     VRLPIVDIAPYDIGGPDQEFGVDVGPVCFL"
     exon            197..399
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            400..450
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            451..564
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            565..645
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            646..699
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            700..753
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            754..807
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            808..861
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            862..915
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            916..969
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            970..1014
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            1015..1068
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            1069..1113
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            1114..1167
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            1168..1266
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            1267..1311
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            1312..1410
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..1464
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            1465..1572
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            1573..1626
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            1627..1725
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            1726..1779
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            1780..1878
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            1879..1932
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            1933..1986
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            1987..2040
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            2041..2094
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            2095..2139
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            2140..2238
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            2239..2346
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            2347..2400
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            2401..2454
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            2455..2508
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            2509..2562
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            2563..2670
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            2671..2724
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            2725..2778
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            2779..2940
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            2941..3048
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            3049..3156
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            3157..3210
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            3211..3318
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            3319..3372
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            3373..3480
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            3481..3534
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            3535..3642
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            3643..3940
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            3941..4128
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            4129..4371
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     exon            4372..5490
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4773..4778
                     /regulatory_class="polyA_signal_sequence"
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /note="hexamer: AATAAA"
     polyA_site      4802
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /note="major polyA site"
     regulatory      5468..5473
                     /regulatory_class="polyA_signal_sequence"
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
                     /note="hexamer: AATAAA"
     polyA_site      5490
                     /gene="COL3A1"
                     /gene_synonym="EDS4A; EDSVASC; PMGEDSV"
ORIGIN      
        1 ggctgagttt tatgacgggc ccggtgctga agggcaggga acaacttgat ggtgctactt
       61 tgaactgctt ttcttttctc ctttttgcac aaagagtctc atgtctgata tttagacatg
      121 atgagctttg tgcaaaaggg gagctggcta cttctcgctc tgcttcatcc cactattatt
      181 ttggcacaac aggaagctgt tgaaggagga tgttcccatc ttggtcagtc ctatgcggat
      241 agagatgtct ggaagccaga accatgccaa atatgtgtct gtgactcagg atccgttctc
      301 tgcgatgaca taatatgtga cgatcaagaa ttagactgcc ccaacccaga aattccattt
      361 ggagaatgtt gtgcagtttg cccacagcct ccaactgctc ctactcgccc tcctaatggt
      421 caaggacctc aaggccccaa gggagatcca ggccctcctg gtattcctgg gagaaatggt
      481 gaccctggta ttccaggaca accagggtcc cctggttctc ctggcccccc tggaatctgt
      541 gaatcatgcc ctactggtcc tcagaactat tctccccagt atgattcata tgatgtcaag
      601 tctggagtag cagtaggagg actcgcaggc tatcctggac cagctggccc cccaggccct
      661 cccggtcccc ctggtacatc tggtcatcct ggttcccctg gatctccagg ataccaagga
      721 ccccctggtg aacctgggca agctggtcct tcaggccctc caggacctcc tggtgctata
      781 ggtccatctg gtcctgctgg aaaagatgga gaatcaggta gacccggacg acctggagag
      841 cgaggattgc ctggacctcc aggtatcaaa ggtccagctg ggatacctgg attccctggt
      901 atgaaaggac acagaggctt cgatggacga aatggagaaa agggtgaaac aggtgctcct
      961 ggattaaagg gtgaaaatgg tcttccaggc gaaaatggag ctcctggacc catgggtcca
     1021 agaggggctc ctggtgagcg aggacggcca ggacttcctg gggctgcagg tgctcggggt
     1081 aatgacggtg ctcgaggcag tgatggtcaa ccaggccctc ctggtcctcc tggaactgcc
     1141 ggattccctg gatcccctgg tgctaagggt gaagttggac ctgcagggtc tcctggttca
     1201 aatggtgccc ctggacaaag aggagaacct ggacctcagg gacacgctgg tgctcaaggt
     1261 cctcctggcc ctcctgggat taatggtagt cctggtggta aaggcgaaat gggtcccgct
     1321 ggcattcctg gagctcctgg actgatggga gcccggggtc ctccaggacc agccggtgct
     1381 aatggtgctc ctggactgcg aggtggtgca ggtgagcctg gtaagaatgg tgccaaagga
     1441 gagcccggac cacgtggtga acgcggtgag gctggtattc caggtgttcc aggagctaaa
     1501 ggcgaagatg gcaaggatgg atcacctgga gaacctggtg caaatgggct tccaggagct
     1561 gcaggagaaa ggggtgcccc tgggttccga ggacctgctg gaccaaatgg catcccagga
     1621 gaaaagggtc ctgctggaga gcgtggtgct ccaggccctg cagggcccag aggagctgct
     1681 ggagaacctg gcagagatgg cgtccctgga ggtccaggaa tgaggggcat gcccggaagt
     1741 ccaggaggac caggaagtga tgggaaacca gggcctcccg gaagtcaagg agaaagtggt
     1801 cgaccaggtc ctcctgggcc atctggtccc cgaggtcagc ctggtgtcat gggcttcccc
     1861 ggtcctaaag gaaatgatgg tgctcctggt aagaatggag aacgaggtgg ccctggagga
     1921 cctggccctc agggtcctcc tggaaagaat ggtgaaactg gacctcaggg acccccaggg
     1981 cctactgggc ctggtggtga caaaggagac acaggacccc ctggtccaca aggattacaa
     2041 ggcttgcctg gtacaggtgg tcctccagga gaaaatggaa aacctgggga accaggtcca
     2101 aagggtgatg ccggtgcacc tggagctcca ggaggcaagg gtgatgctgg tgcccctggt
     2161 gaacgtggac ctcctggatt ggcaggggcc ccaggactta gaggtggagc tggtccccct
     2221 ggtcccgaag gaggaaaggg tgctgctggt cctcctgggc cacctggtgc tgctggtact
     2281 cctggtctgc aaggaatgcc tggagaaaga ggaggtcttg gaagtcctgg tccaaagggt
     2341 gacaagggtg aaccaggcgg tccaggtgct gatggtgtcc cagggaaaga tggcccaagg
     2401 ggtcctactg gtcctattgg tcctcctggc ccagctggcc agcctggaga taagggtgaa
     2461 ggtggtgccc ccggacttcc aggtatagct ggacctcgtg gtagccctgg tgagagaggt
     2521 gaaactggcc ctccaggacc tgctggtttc cctggtgctc ctggacagaa tggtgaacct
     2581 ggtggtaaag gagaaagagg ggctccgggt gagaaaggtg aaggaggccc tcctggagtt
     2641 gcaggacccc ctggaggttc tggacctgct ggtcctcctg gtccccaagg tgtcaaaggt
     2701 gaacgtggca gtcctggtgg acctggtgct gctggcttcc ctggtgctcg tggtcttcct
     2761 ggtcctcctg gtagtaatgg taacccagga cccccaggtc ccagcggttc tccaggcaag
     2821 gatgggcccc caggtcctgc gggtaacact ggtgctcctg gcagccctgg agtgtctgga
     2881 ccaaaaggtg atgctggcca accaggagag aagggatcgc ctggtgccca gggcccacca
     2941 ggagctccag gcccacttgg gattgctggg atcactggag cacggggtct tgcaggacca
     3001 ccaggcatgc caggtcctag gggaagccct ggccctcagg gtgtcaaggg tgaaagtggg
     3061 aaaccaggag ctaacggtct cagtggagaa cgtggtcccc ctggacccca gggtcttcct
     3121 ggtctggctg gtacagctgg tgaacctgga agagatggaa accctggatc agatggtctt
     3181 ccaggccgag atggatctcc tggtggcaag ggtgatcgtg gtgaaaatgg ctctcctggt
     3241 gcccctggcg ctcctggtca tccaggccca cctggtcctg tcggtccagc tggaaagagt
     3301 ggtgacagag gagaaagtgg ccctgctggc cctgctggtg ctcccggtcc tgctggttcc
     3361 cgaggtgctc ctggtcctca aggcccacgt ggtgacaaag gtgaaacagg tgaacgtgga
     3421 gctgctggca tcaaaggaca tcgaggattc cctggtaatc caggtgcccc aggttctcca
     3481 ggccctgctg gtcagcaggg tgcaatcggc agtccaggac ctgcaggccc cagaggacct
     3541 gttggaccca gtggacctcc tggcaaagat ggaaccagtg gacatccagg tcccattgga
     3601 ccaccagggc ctcgaggtaa cagaggtgaa agaggatctg agggctcccc aggccaccca
     3661 gggcaaccag gccctcctgg acctcctggt gcccctggtc cttgctgtgg tggtgttgga
     3721 gccgctgcca ttgctgggat tggaggtgaa aaagctggcg gttttgcccc gtattatgga
     3781 gatgaaccaa tggatttcaa aatcaacacc gatgagatta tgacttcact caagtctgtt
     3841 aatggacaaa tagaaagcct cattagtcct gatggttctc gtaaaaaccc cgctagaaac
     3901 tgcagagacc tgaaattctg ccatcctgaa ctcaagagtg gagaatactg ggttgaccct
     3961 aaccaaggat gcaaattgga tgctatcaag gtattctgta atatggaaac tggggaaaca
     4021 tgcataagtg ccaatccttt gaatgttcca cggaaacact ggtggacaga ttctagtgct
     4081 gagaagaaac acgtttggtt tggagagtcc atggatggtg gttttcagtt tagctacggc
     4141 aatcctgaac ttcctgaaga tgtccttgat gtgcatctgg cattccttcg acttctctcc
     4201 agccgagctt cccagaacat cacatatcac tgcaaaaata gcattgcata catggatcag
     4261 gccagtggaa atgtaaagaa ggccctgaag ctgatggggt caaatgaagg tgaattcaag
     4321 gctgaaggaa atagcaaatt cacctacaca gttctggagg atggttgcac gaaacacact
     4381 ggggaatgga gcaaaacagt ctttgaatat cgaacacgca aggctgtgag actacctatt
     4441 gtagatattg caccctatga cattggtggt cctgatcaag aatttggtgt ggacgttggc
     4501 cctgtttgct ttttataaac caaactctat ctgaaatccc aacaaaaaaa atttaactcc
     4561 atatgtgttc ctcttgttct aatcttgtca accagtgcaa gtgaccgaca aaattccagt
     4621 tatttatttc caaaatgttt ggaaacagta taatttgaca aagaaaaatg atacttctct
     4681 ttttttgctg ttccaccaaa tacaattcaa atgctttttg ttttattttt ttaccaattc
     4741 caatttcaaa atgtctcaat ggtgctataa taaataaact tcaacactct ttatgataac
     4801 aacactgtgt tatattcttt gaatcctagc ccatctgcag agcaatgact gtgctcacca
     4861 gtaaaagata acctttcttt ctgaaatagt caaatacgaa attagaaaag ccctccctat
     4921 tttaactacc tcaactggtc agaaacacag attgtattct atgagtccca gaagatgaaa
     4981 aaaattttat acgttgataa aacttataaa tttcattgat taatctcctg gaagattggt
     5041 ttaaaaagaa aagtgtaatg caagaattta aagaaatatt tttaaagcca caattatttt
     5101 aatattggat atcaactgct tgtaaaggtg ctcctctttt ttcttgtcat tgctggtcaa
     5161 gattactaat atttgggaag gctttaaaga cgcatgttat ggtgctaatg tactttcact
     5221 tttaaactct agatcagaat tgttgacttg cattcagaac ataaatgcac aaaatctgta
     5281 catgtctccc atcagaaaga ttcattggca tgccacaggg gattctcctc cttcatcctg
     5341 taaaggtcaa caataaaaac caaattatgg ggctgctttt gtcacactag catagagaat
     5401 gtgttgaaat ttaactttgt aagcttgtat gtggttgttg atcttttttt tccttacaga
     5461 cacccataat aaaatatcat attaaaattc
//
