LOCUS       NM_152888               6394 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens collagen type XXII alpha 1 chain (COL22A1), mRNA.
ACCESSION   NM_152888 XM_291257
VERSION     NM_152888.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6394)
  AUTHORS   Sun,Z., Cieszczyk,P., Lulinska,E., Dzitkowska-Zabielska,M.,
            Johne,M., Huminska-Lisowska,K., Michalowska-Sawczyn,M., Ficek,K.,
            Leonska-Duniec,A., Mastalerz,A., Janczyk,A. and Marek,S.
  TITLE     Are COL22A1 Gene Polymorphisms rs11784270 and rs6577958 Associated
            with Susceptibility to a Non-Contact Anterior Cruciate Ligament
            Injury in Polish Athletes?
  JOURNAL   Int J Environ Res Public Health 20 (1), 515 (2022)
   PUBMED   36612834
  REMARK    GeneRIF: Are COL22A1 Gene Polymorphisms rs11784270 and rs6577958
            Associated with Susceptibility to a Non-Contact Anterior Cruciate
            Ligament Injury in Polish Athletes?
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6394)
  AUTHORS   Miyamoto-Mikami,E., Kumagai,H., Kikuchi,N., Kamiya,N., Miyamoto,N.
            and Fuku,N.
  TITLE     eQTL variants in COL22A1 are associated with muscle injury in
            athletes
  JOURNAL   Physiol Genomics 52 (12), 588-589 (2020)
   PUBMED   33166209
  REMARK    GeneRIF: eQTL variants in COL22A1 are associated with muscle injury
            in athletes.
REFERENCE   3  (bases 1 to 6394)
  AUTHORS   Watanabe,T., Baker Frost,D.A., Mlakar,L., Heywood,J., da
            Silveira,W.A., Hardiman,G. and Feghali-Bostwick,C.
  TITLE     A Human Skin Model Recapitulates Systemic Sclerosis Dermal Fibrosis
            and Identifies COL22A1 as a TGFbeta Early Response Gene that
            Mediates Fibroblast to Myofibroblast Transition
  JOURNAL   Genes (Basel) 10 (2), 75 (2019)
   PUBMED   30678304
  REMARK    GeneRIF: COL22A1 mediates fibroblast to myofibroblast transition in
            systemic sclerosis.  Silencing of COL22A1 significantly reduced
            TGFbeta-induced ACTA2 expression.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6394)
  AUTHORS   Ton,Q.V., Leino,D., Mowery,S.A., Bredemeier,N.O., Lafontant,P.J.,
            Lubert,A., Gurung,S., Farlow,J.L., Foroud,T.M., Broderick,J. and
            Sumanas,S.
  TITLE     Collagen COL22A1 maintains vascular stability and mutations in
            COL22A1 are potentially associated with intracranial aneurysms
  JOURNAL   Dis Model Mech 11 (12) (2018)
   PUBMED   30541770
  REMARK    GeneRIF: COL22A1 is required to maintain vascular integrity.  These
            data further suggest that mutations in COL22A1 could be one of the
            risk factors for intracranial aneurysms in humans.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6394)
  AUTHORS   Zwolanek,D., Veit,G., Eble,J.A., Gullberg,D., Ruggiero,F.,
            Heino,J., Meier,M., Stetefeld,J. and Koch,M.
  TITLE     Collagen XXII binds to collagen-binding integrins via the novel
            motifs GLQGER and GFKGER
  JOURNAL   Biochem J 459 (1), 217-227 (2014)
   PUBMED   24428702
  REMARK    GeneRIF: Collage XXII interacts with collagen-binding integrins via
            the protein motifs GLQGER and GFKGER.
REFERENCE   6  (bases 1 to 6394)
  AUTHORS   Charvet,B., Guiraud,A., Malbouyres,M., Zwolanek,D., Guillon,E.,
            Bretaud,S., Monnot,C., Schulze,J., Bader,H.L., Allard,B., Koch,M.
            and Ruggiero,F.
  TITLE     Knockdown of col22a1 gene in zebrafish induces a muscular dystrophy
            by disruption of the myotendinous junction
  JOURNAL   Development 140 (22), 4602-4613 (2013)
   PUBMED   24131632
REFERENCE   7  (bases 1 to 6394)
  AUTHORS   Pattaro,C., De Grandi,A., Vitart,V., Hayward,C., Franke,A.,
            Aulchenko,Y.S., Johansson,A., Wild,S.H., Melville,S.A., Isaacs,A.,
            Polasek,O., Ellinghaus,D., Kolcic,I., Nothlings,U., Zgaga,L.,
            Zemunik,T., Gnewuch,C., Schreiber,S., Campbell,S., Hastie,N.,
            Boban,M., Meitinger,T., Oostra,B.A., Riegler,P., Minelli,C.,
            Wright,A.F., Campbell,H., van Duijn,C.M., Gyllensten,U.,
            Wilson,J.F., Krawczak,M., Rudan,I. and Pramstaller,P.P.
  CONSRTM   EUROSPAN consortium
  TITLE     A meta-analysis of genome-wide data from five European isolates
            reveals an association of COL22A1, SYT1, and GABRR2 with serum
            creatinine level
  JOURNAL   BMC Med Genet 11, 41 (2010)
   PUBMED   20222955
  REMARK    GeneRIF: association between serum creatinine level and
            polymorphisms in the collagen type XXII alpha 1 (COL22A1) gene, on
            chromosome 8, and in the synaptotagmin-1 (SYT1) gene, on chromosome
            12
            GeneRIF: Observational study, meta-analysis, and genome-wide
            association study of gene-disease association. (HuGE Navigator)
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 6394)
  AUTHORS   Carter,E.M. and Raggio,C.L.
  TITLE     Genetic and orthopedic aspects of collagen disorders
  JOURNAL   Curr Opin Pediatr 21 (1), 46-54 (2009)
   PUBMED   19253462
  REMARK    Review article
REFERENCE   9  (bases 1 to 6394)
  AUTHORS   Ricard-Blum,S. and Ruggiero,F.
  TITLE     The collagen superfamily: from the extracellular matrix to the cell
            membrane
  JOURNAL   Pathol Biol (Paris) 53 (7), 430-442 (2005)
   PUBMED   16085121
  REMARK    Review article
REFERENCE   10 (bases 1 to 6394)
  AUTHORS   Koch,M., Schulze,J., Hansen,U., Ashwodt,T., Keene,D.R.,
            Brunken,W.J., Burgeson,R.E., Bruckner,P. and Bruckner-Tuderman,L.
  TITLE     A novel marker of tissue junctions, collagen XXII
  JOURNAL   J Biol Chem 279 (21), 22514-22521 (2004)
   PUBMED   15016833
  REMARK    GeneRIF: collagen XXII is the first specific extracellular matrix
            protein present only at tissue junctions
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC068476.13, BX094632.1,
            AF406780.1 and BC144535.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_152888.2.
            
            Summary: This gene encodes member of the collagen family which is
            thought to contribute to the stabilization of myotendinous
            junctions and strengthen skeletal muscle attachments during
            contractile activity. It belongs to the fibril-associated collagens
            with interrupted triple helix (FACIT) subset of the collagen
            superfamily, which associate with collagen fibers through their
            C-terminal collagenous domains and mediate protein-protein
            interactions through their N-terminal noncollagenous domains. The
            encoded protein is deposited in the basement membrane zone of the
            myotendinous junction which is present only at the tissue junctions
            of muscles, tendons, the heart, articular cartilage, and skin. A
            knockdown of the orthologous zebrafish gene induces a muscular
            dystrophy by disruption of the myotendinous junction. [provided by
            RefSeq, May 2017].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF406780.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000303045.11/ ENSP00000303153.6
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-35                AC068476.13        35692-35726
            36-286              BX094632.1         1-251
            287-287             AC068476.13        35978-35978
            288-1452            AF406780.1         240-1404
            1453-1453           AC068476.13        123064-123064
            1454-3434           AF406780.1         1406-3386
            3435-3435           BC144535.1         2118-2118
            3436-4094           AF406780.1         3388-4046
            4095-4095           BC144535.1         2718-2718
            4096-6394           AF406780.1         4048-6346
FEATURES             Location/Qualifiers
     source          1..6394
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q24.23-q24.3"
     gene            1..6394
                     /gene="COL22A1"
                     /note="collagen type XXII alpha 1 chain"
                     /db_xref="GeneID:169044"
                     /db_xref="HGNC:HGNC:22989"
                     /db_xref="MIM:610026"
     exon            1..423
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            424..586
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     CDS             496..5376
                     /gene="COL22A1"
                     /note="collagen alpha-1(XXII) chain"
                     /codon_start=1
                     /product="collagen alpha-1(XXII) chain precursor"
                     /protein_id="NP_690848.1"
                     /db_xref="CCDS:CCDS6376.1"
                     /db_xref="GeneID:169044"
                     /db_xref="HGNC:HGNC:22989"
                     /db_xref="MIM:610026"
                     /translation="MAGLRGNAVAGLLWMLLLWSGGGGCQAQRAGCKSVHYDLVFLLD
                     TSSSVGKEDFEKVRQWVANLVDTFEVGPDRTRVGVVRYSDRPTTAFELGLFGSQEEVK
                     AAARRLAYHGGNTNTGDALRYITARSFSPHAGGRPRDRAYKQVAILLTDGRSQDLVLD
                     AAAAAHRAGIRIFAVGVGEALKEELEEIASEPKSAHVFHVSDFNAIDKIRGKLRRRLC
                     ENVLCPSVRVEGDRFKHTNGGTKEITGFDLMDLFSVKEILGKRENGAQSSYVRMGSFP
                     VVQSTEDVFPQGLPDEYAFVTTFRFRKTSRKEDWYIWQVIDQYSIPQVSIRLDGENKA
                     VEYNAVGAMKDAVRVVFRGSRVNDLFDRDWHKMALSIQAQNVSLHIDCALVQTLPIEE
                     RENIDIQGKTVIGKRLYDSVPIDFDLQRIVIYCDSRHAELETCCDIPSGPCQVTVVTE
                     PPPPPPPQRPPTPGSEQIGFLKTINCSCPAGEKGEMGVAGPMGLPGPKGDIGAIGPVG
                     APGPKGEKGDVGIGPFGQGEKGEKGSLGLPGPPGRDGSKGMRGEPGELGEPGLPGEVG
                     MRGPQGPPGLPGPPGRVGAPGLQGERGEKGTRGEKGERGLDGFPGKPGDTGQQGRPGP
                     SGVAGPQGEKGDVGPAGPPGVPGSVVQQEGLKGEQGAPGPRGHQGAPGPPGARGPIGP
                     EGRDGPPGLQGLRGKKGDMGPPGIPGLLGLQGPPGPPGVPGPPGPGGSPGLPGEIGFP
                     GKPGPPGPTGPPGKDGPNGPPGPPGTKGEPGERGEDGLPGKPGLRGEIGEQGLAGRPG
                     EKGEAGLPGAPGFPGVRGEKGDQGEKGELGLPGLKGDRGEKGEAGPAGPPGLPGTTSL
                     FTPHPRMPGEQGPKGEKGDPGLPGEPGLQGRPGELGPQGPTGPPGAKGQEGAHGAPGA
                     AGNPGAPGHVGAPGPSGPPGSVGAPGLRGTPGKDGERGEKGAAGEEGSPGPVGPRGDP
                     GAPGLPGPPGKGKDGEPGLRGSPGLPGPLGTKAACGKVRGSENCALGGQCVKGDRGAP
                     GIPGSPGSRGDPGIGVAGPPGPSGPPGDKGSPGSRGLPGFPGPQGPAGRDGAPGNPGE
                     RGPPGKPGLSSLLSPGDINLLAKDVCNDCPPGPPGLPGLPGFKGDKGVPGKPGREGTE
                     GKKGEAGPPGLPGPPGIAGPQGSQGERGADGEVGQKGDQGHPGVPGFMGPPGNPGPPG
                     ADGIAGAAGPPGIQGSPGKEGPPGPQGPSGLPGIPGEEGKEGRDGKPGPPGEPGKAGE
                     PGLPGPEGARGPPGFKGHTGDSGAPGPRGESGAMGLPGQEGLPGKDGDTGPTGPQGPQ
                     GPRGPPGKNGSPGSPGEPGPSGTPGQKGSKGENGSPGLPGFLGPRGPPGEPGEKGVPG
                     KEGVPGKPGEPGFKGERGDPGIKGDKGPPGGKGQPGDPGIPGHKGHTGLMGPQGLPGE
                     NGPVGPPGPPGQPGFPGLRGESPSMETLRRLIQEELGKQLETRLAYLLAQMPPAYMKS
                     SQGRPGPPGPPGKDGLPGRAGPMGEPGRPGQGGLEGPSGPIGPKGERGAKGDPGAPGV
                     GLRGEMGPPGIPGQPGEPGYAKDGLPGIPGPQGETGPAGHPGLPGPPGPPGQCDPSQC
                     AYFASLAARPGNVKGP"
     exon            587..1153
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1154..1228
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1229..1340
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1341..1464
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1465..1740
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1741..1821
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1822..1944
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1945..1989
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1990..2052
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2053..2091
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2092..2145
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2146..2199
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2200..2253
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2254..2298
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2299..2352
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2353..2397
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2398..2442
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2443..2472
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2473..2526
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2527..2580
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2581..2634
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2635..2688
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2689..2742
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2743..2796
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2797..2850
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2851..2895
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2896..2958
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2959..3012
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            3013..3054
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            3055..3087
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            3088..3141
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            3142..3195
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            3196..3249
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            3250..3303
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            3304..3357
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            3358..3462
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            3463..3507
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            3508..3567
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            3568..3645
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            3646..3681
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            3682..3735
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            3736..3780
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            3781..3828
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            3829..3942
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            3943..3996
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            3997..4050
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            4051..4104
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            4105..4158
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            4159..4212
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            4213..4266
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            4267..4320
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            4321..4365
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            4366..4419
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            4420..4473
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            4474..4527
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            4528..4599
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            4600..4635
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            4636..4680
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            4681..4860
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            4861..4927
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            4928..5110
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            5111..5188
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     exon            5189..6394
                     /gene="COL22A1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6369..6374
                     /regulatory_class="polyA_signal_sequence"
                     /gene="COL22A1"
                     /note="hexamer: AATAAA"
     polyA_site      6394
                     /gene="COL22A1"
                     /note="major polyA site"
ORIGIN      
        1 agcttgcccc tggggaggct ccagacagct ttgttcctgg gcagtggcgg ggctggccga
       61 ggctgcggcg tttctcccca aggaagtgtc ttctccgctt tccctgttct tctgtttctc
      121 acacactttc tatctcattc tgtaactttc aagcctttcc ttctaactgt atgtatttag
      181 ttacttgttt tcaagctggt tccctctctg gccccctggc ctggggaaag cctccacact
      241 tactgcgggt cttgtttaga gtctgagttt gtgagattat ttgggggaga gtgggcgagt
      301 ggctgacagg tgacccccag gaggaggatt cctggggctg gtgtcttctc ccagctgctg
      361 cttccagtgg gcctgggccc aggactggac ctccgctggc acccctgagt gcctccctgc
      421 caggccatgc tgctgtagac cctaacagcg tctcttcctg gccaagagaa gcctgtcccc
      481 aagaacagga gagccatggc cggcctccga gggaacgctg tggctggcct cctctggatg
      541 ctgctgctgt ggagtggggg cggcggctgc caggctcagc gggcaggttg caaaagtgtc
      601 cactacgatc tggtcttcct cctggacacc tcctccagcg tgggcaagga ggactttgag
      661 aaggtccggc agtgggtggc caacctggtg gacaccttcg aggtgggccc cgaccgcacc
      721 cgtgtggggg tcgtgcgcta cagcgaccgg cccaccacgg ccttcgagtt gggactcttt
      781 ggctcgcagg aggaggtcaa ggcggctgcc cggcgtctcg cctaccacgg gggcaacacc
      841 aacacgggag acgcgctccg ctacatcacg gcccgcagct tctccccaca cgccggcggc
      901 cgccccaggg accgcgccta caagcaggtg gccatcctgc tcaccgacgg ccgcagccag
      961 gacctggtgc tggacgccgc ggcggcagcc caccgcgctg gcatccgcat ctttgccgtg
     1021 ggcgtgggcg aggcactcaa ggaggagctg gaggagatcg cctcagagcc caagtccgcc
     1081 cacgtcttcc acgtgtccga cttcaatgcc atcgacaaga tccggggcaa gctgcggcgc
     1141 cgtctttgtg aaaatgtgct ctgtcctagc gttcgtgtag aaggagatcg ctttaagcac
     1201 accaatggag gaaccaagga aatcacaggt tttgacctga tggatttgtt cagtgtgaag
     1261 gaaatcttgg ggaagagaga gaatggagct cagagttcct atgtacggat gggatccttc
     1321 cctgtggtgc aaagtactga ggatgtgttc ccccaaggtt tacctgatga gtacgccttt
     1381 gtcacaacct tccggttcag gaaaacctct cggaaggaag actggtatat ctggcaggtc
     1441 atcgaccagt acagcatccc acaggtctcc atccggctgg atggtgaaaa caaggcagtc
     1501 gagtacaacg ctgtgggtgc catgaaagat gctgtcaggg tggtcttccg aggttctcgg
     1561 gtcaatgacc tctttgaccg ggactggcac aagatggccc tgagcatcca ggcccagaac
     1621 gtctccctgc acattgactg tgcgctggtg cagacactac ccatcgagga acgggagaac
     1681 attgacatcc agggcaagac tgtgattggc aagcgcctct acgacagtgt gcccattgac
     1741 tttgacctac agcggattgt gatctattgt gactcgagac acgcagaatt ggagacttgt
     1801 tgtgatatcc cctcgggtcc gtgccaggtg accgtggtga cagagcctcc acctccaccc
     1861 ccaccccagc ggcctcccac cccaggcagt gaacagattg ggtttttgaa gaccatcaac
     1921 tgctcctgcc cagctggaga gaagggtgaa atgggagttg ctggccccat ggggctccct
     1981 ggtccaaagg gagacatagg agccattggg ccggttggcg ctcctggacc taagggagag
     2041 aaaggtgatg tgggcatagg accttttggc caaggggaaa agggtgaaaa gggttccctg
     2101 ggcctgcccg gcccccctgg gagagacggc agcaaaggca tgagagggga gccaggagag
     2161 ctgggagagc cggggctgcc gggtgaggtc ggcatgcggg ggccccaagg accacctgga
     2221 ctccccggac ctcctggacg tgtcggagct cctggtctcc aaggagaacg aggtgaaaag
     2281 ggaactcgag gagaaaaggg agagcgaggc ctggatggat tccctgggaa gcctggggac
     2341 acaggacagc agggcaggcc cggcccttct ggtgtggcag gaccccaggg agaaaagggt
     2401 gacgtgggac ctgcggggcc acctggtgta ccaggctcag tggtgcagca agagggcttg
     2461 aaaggggaac agggagctcc aggacccaga ggtcaccaag gcgcccccgg tcctccagga
     2521 gctcggggtc caataggccc agaaggcagg gatggacctc ctggtttgca aggtctccga
     2581 gggaagaaag gtgacatggg accacctgga atccctggat tgctggggct gcagggccct
     2641 ccaggacccc ctggtgtccc aggcccccct ggaccgggag gttctccggg tttgcctgga
     2701 gagatcggct tcccgggaaa gcctggacct cctgggccca cgggaccccc tggaaaggac
     2761 gggccaaatg gaccaccagg tccgccagga accaagggag aaccaggaga aagaggggaa
     2821 gatggtctgc ctggaaaacc aggccttcgg ggagaaattg gggagcaggg cctggcaggc
     2881 cgacctggag agaagggaga agcaggcctc ccaggggctc caggcttccc aggtgtgaga
     2941 ggagagaaag gagaccaggg agaaaaaggt gaactgggac ttccaggact gaaaggtgac
     3001 cgaggtgaaa agggtgaagc tggtcctgca ggccctcccg ggttacctgg aactacatcc
     3061 ctgttcacac cacatccacg gatgcccgga gaacaagggc ccaaaggaga gaagggcgat
     3121 ccaggcctgc ctggggaacc gggactgcag ggccgtcctg gagaattggg gcctcaggga
     3181 cccactggac caccgggtgc caagggacag gaaggtgcac atggggctcc tggagcagct
     3241 ggaaaccccg gtgctcccgg acatgtcggt gcccccggtc ccagtggccc tccaggaagt
     3301 gtgggtgctc ccggcctcag aggcacccca gggaaagatg gggagcgtgg tgagaagggt
     3361 gcagcggggg aagaaggcag cccagggcca gttggtccca ggggagatcc tggtgctcct
     3421 gggctccctg ggccacccgg aaaagggaag gatggagagc cgggactccg tggatcacct
     3481 ggactccctg gacccctagg aaccaaggct gcttgcggaa aagtcagagg gtcagaaaac
     3541 tgtgcactgg gagggcaatg tgttaagggg gatcgaggag ctcctgggat ccctggttct
     3601 cctggcagcc gtggtgaccc aggcattggg gttgctggcc ctcctggccc ttccggacca
     3661 ccaggagaca aaggatcccc gggatcacga ggcttacctg gattccctgg cccccagggc
     3721 ccagccggcc gggacggtgc accaggaaat ccaggagaaa gagggcctcc tggcaagccg
     3781 ggcctctctt cactactgtc tccaggggac ataaatctct tggctaagga tgtgtgcaat
     3841 gactgccctc ctggcccccc aggcctccct ggtctaccag gttttaaagg ggacaaaggt
     3901 gtcccaggaa agccagggag agaaggcaca gaagggaaaa agggagaggc tgggcctcca
     3961 ggcctaccag ggcccccagg aatagctgga ccacagggaa gtcaaggaga acgtggtgca
     4021 gatggtgagg ttgggcagaa aggtgatcag ggtcatcctg gagttccagg tttcatgggg
     4081 cccccaggga accctgggcc accaggggca gatggaattg caggagctgc tggaccacca
     4141 ggaatccaag ggtcacctgg gaaagaaggc cctcctggcc cccaaggccc atctggatta
     4201 cccggaatcc caggagaaga aggcaaagag ggcagagatg gaaagccggg tccccctgga
     4261 gagccgggca aagcaggaga gccaggtcta ccaggaccag agggtgcccg aggcccacct
     4321 ggcttcaagg gacacacagg cgattctggt gcacccggtc cccggggaga gtctggtgcc
     4381 atggggcttc ctggtcagga agggttacca ggaaaagatg gtgacactgg acccactggg
     4441 ccacagggtc cccaaggacc aaggggccca ccgggcaaga atggatcacc gggatctcca
     4501 ggagagcctg gcccttcagg aacccctggc cagaaaggaa gcaaagggga aaatggcagc
     4561 ccaggacttc ctggcttcct gggtccccgt gggcctccgg gagaaccagg agagaaagga
     4621 gtcccaggca aggagggggt ccctgggaag cctggagagc ctggattcaa aggagaaagg
     4681 ggagatcctg ggatcaaagg tgacaaagga cctcctggtg gaaaaggcca gcctggggac
     4741 cctggaatcc caggccacaa aggccacaca ggcctgatgg gtccccaagg actacctggg
     4801 gagaatggac cagttggacc cccagggcct ccaggccagc cgggatttcc aggactgagg
     4861 ggggagtctc catccatgga aaccctgcgt cggcttattc aagaagagct ggggaagcag
     4921 cttgaaacca gactcgccta cctcctggcc cagatgcccc cggcgtacat gaagtcatct
     4981 caaggcagac ctgggccccc agggccccct ggaaaagatg ggcttccagg ccgggccggc
     5041 cccatggggg agccaggtcg tcctgggcag gggggtctgg aaggaccctc tggacccata
     5101 ggtcccaaag gtgagcgagg agccaaaggt gacccaggtg cacctggagt tggcctccga
     5161 ggcgagatgg gaccccctgg aatcccaggt caacccgggg aacctggcta tgctaaagat
     5221 ggacttcctg ggatccctgg ccctcaaggg gagacaggac cagctggaca tcctggcctc
     5281 ccaggacctc ccggtccccc aggccaatgt gacccttccc agtgtgccta cttcgccagc
     5341 cttgctgccc ggccgggtaa tgtgaagggt ccctaaagga ctctggaaag ccagaagact
     5401 gcagtggatt tctgaaactt gaactcagag cccagtggga agccagaggt cttgaaagac
     5461 ttcagccatg tgttcctttt ttttttcttt cttttatcgt ttgctttttg ttttattttc
     5521 ttgagagacc tcaaaattat taaatccaac agacgctgcc ggtcggtcag attattatta
     5581 atattattgt tgttgttaat tattattatt atttcatatg ctgatgcttt gtgagttctt
     5641 ttccactcct ttaaagttgg gaaaacttga ttcgtggggc aggagattgt ttcttcattc
     5701 ttctgacagc ccccatctga cgcgtaactg cccattttaa ggaaactctt ggtgctacaa
     5761 aaccctgacc agacacttgg caaatttacc tctttcttca aaagaaaaac tttaagaaaa
     5821 tgagccaatg ggcttcattc tcagtcatgc ccggagatca cccaggagaa ataatacaaa
     5881 caccaccact gtccagagag agtaaagaag cagaaagaga aagaatttgc aaccatgagg
     5941 aatgttccca cctcccgacg ggacgtgcat ttggaaaaca cagaatcagc cctcagggtg
     6001 cactccagcc acctcagtgc tctaagctca cagaagtgaa ataatgtctg tgggttggca
     6061 atggctttgt gggatcatat gtcttggcca aagatgggaa aacctatgtt gaagaggcag
     6121 cccttgagtg ttaatttgtc ttctaaactg tgtaaggccc cttcaagttc ctcttgttgg
     6181 tttcaattat attaattata aaacaagtgg atgtggtgac catccacttg tgtttcccta
     6241 atgatgggca gttggccagg gcactgacca gagctgggaa atttgtatct ccaaggcggc
     6301 tctgtctctg aaataaatgg catcaagtgc atgtgtgtat gcgacatgcc ctgcctgaac
     6361 aggtgctcaa taaatccaag tttccttctc ttga
//
