LOCUS       NM_001379500            5408 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens collagen type XVIII alpha 1 chain (COL18A1),
            transcript variant 4, mRNA.
ACCESSION   NM_001379500
VERSION     NM_001379500.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Rinta-Jaskari,M.M., Naillat,F., Ruotsalainen,H.J., Koivunen,J.T.,
            Sasaki,T., Pietila,I., Elamaa,H.P., Kaur,I., Manninen,A.,
            Vainio,S.J. and Pihlajaniemi,T.A.
  TITLE     Temporally and spatially regulated collagen XVIII isoforms are
            involved in ureteric tree development via the TSP1-like domain
  JOURNAL   Matrix Biol 115, 139-159 (2023)
   PUBMED   36623578
  REMARK    GeneRIF: Temporally and spatially regulated collagen XVIII isoforms
            are involved in ureteric tree development via the TSP1-like domain.
REFERENCE   2  (bases 1 to 5408)
  AUTHORS   Haghighi,A., Tiwari,A., Piri,N., Nurnberg,G., Saleh-Gohari,N.,
            Haghighi,A., Neidhardt,J., Nurnberg,P. and Berger,W.
  TITLE     Homozygosity mapping and whole exome sequencing reveal a novel
            homozygous COL18A1 mutation causing Knobloch syndrome
  JOURNAL   PLoS One 9 (11), e112747 (2014)
   PUBMED   25392994
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5408)
  AUTHORS   Seppinen,L. and Pihlajaniemi,T.
  TITLE     The multiple functions of collagen XVIII in development and disease
  JOURNAL   Matrix Biol 30 (2), 83-92 (2011)
   PUBMED   21163348
  REMARK    Review article
REFERENCE   4  (bases 1 to 5408)
  AUTHORS   Heljasvaara,R., Nyberg,P., Luostarinen,J., Parikka,M., Heikkila,P.,
            Rehn,M., Sorsa,T., Salo,T. and Pihlajaniemi,T.
  TITLE     Generation of biologically active endostatin fragments from human
            collagen XVIII by distinct matrix metalloproteases
  JOURNAL   Exp Cell Res 307 (2), 292-304 (2005)
   PUBMED   15950618
  REMARK    GeneRIF: Biologically active endostatin, generated by matrix
            metalloproteases from collagen XVIII, may participate in the
            inhibition of endothelial cell proliferation, migration and
            angiogenesis.
REFERENCE   5  (bases 1 to 5408)
  AUTHORS   Elamaa,H., Snellman,A., Rehn,M., Autio-Harmainen,H. and
            Pihlajaniemi,T.
  TITLE     Characterization of the human type XVIII collagen gene and
            proteolytic processing and tissue location of the variant
            containing a frizzled motif
  JOURNAL   Matrix Biol 22 (5), 427-442 (2003)
   PUBMED   14614989
REFERENCE   6  (bases 1 to 5408)
  AUTHORS   Felbor,U., Dreier,L., Bryant,R.A., Ploegh,H.L., Olsen,B.R. and
            Mothes,W.
  TITLE     Secreted cathepsin L generates endostatin from collagen XVIII
  JOURNAL   EMBO J 19 (6), 1187-1194 (2000)
   PUBMED   10716919
REFERENCE   7  (bases 1 to 5408)
  AUTHORS   Saarela,J., Ylikarppa,R., Rehn,M., Purmonen,S. and Pihlajaniemi,T.
  TITLE     Complete primary structure of two variant forms of human type XVIII
            collagen and tissue-specific differences in the expression of the
            corresponding transcripts
  JOURNAL   Matrix Biol 16 (6), 319-328 (1998)
   PUBMED   9503365
REFERENCE   8  (bases 1 to 5408)
  AUTHORS   Standker,L., Schrader,M., Kanse,S.M., Jurgens,M., Forssmann,W.G.
            and Preissner,K.T.
  TITLE     Isolation and characterization of the circulating form of human
            endostatin
  JOURNAL   FEBS Lett 420 (2-3), 129-133 (1997)
   PUBMED   9459295
REFERENCE   9  (bases 1 to 5408)
  AUTHORS   Oh,S.P., Kamagata,Y., Muragaki,Y., Timmons,S., Ooshima,A. and
            Olsen,B.R.
  TITLE     Isolation and sequencing of cDNAs for proteins with multiple
            domains of Gly-Xaa-Yaa repeats identify a distinct family of
            collagenous proteins
  JOURNAL   Proc Natl Acad Sci U S A 91 (10), 4229-4233 (1994)
   PUBMED   8183893
REFERENCE   10 (bases 1 to 5408)
  AUTHORS   Oh,S.P., Warman,M.L., Seldin,M.F., Cheng,S.D., Knoll,J.H.,
            Timmons,S. and Olsen,B.R.
  TITLE     Cloning of cDNA and genomic DNA encoding human type XVIII collagen
            and localization of the alpha 1(XVIII) collagen gene to mouse
            chromosome 10 and human chromosome 21
  JOURNAL   Genomics 19 (3), 494-499 (1994)
   PUBMED   8188291
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BX322562.1 and BX322561.1.
            
            Summary: This gene encodes the alpha chain of type XVIII collagen.
            This collagen is one of the multiplexins, extracellular matrix
            proteins that contain multiple triple-helix domains (collagenous
            domains) interrupted by non-collagenous domains. A long isoform of
            the protein has an N-terminal domain that is homologous to the
            extracellular part of frizzled receptors. Proteolytic processing at
            several endogenous cleavage sites in the C-terminal domain results
            in production of endostatin, a potent antiangiogenic protein that
            is able to inhibit angiogenesis and tumor growth. Mutations in this
            gene are associated with Knobloch syndrome. The main features of
            this syndrome involve retinal abnormalities, so type XVIII collagen
            may play an important role in retinal structure and in neural tube
            closure. Alternative splicing results in multiple transcript
            variants. [provided by RefSeq, Dec 2014].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF018082.1, BC063833.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000651438.1/ ENSP00000498485.1
            RefSeq Select criteria :: based on manual assertion, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-77                BX322562.1         22400-22476
            78-172              BX322562.1         22614-22708
            173-717             BX322561.1         27324-27868
            718-804             BX322561.1         32977-33063
            805-864             BX322561.1         34558-34617
            865-994             BX322561.1         35433-35562
            995-1071            BX322561.1         36493-36569
            1072-1287           BX322561.1         36832-37047
            1288-1314           BX322561.1         37409-37435
            1315-1377           BX322561.1         38984-39046
            1378-1464           BX322561.1         39152-39238
            1465-1518           BX322561.1         39549-39602
            1519-1677           BX322561.1         39782-39940
            1678-1740           BX322561.1         41045-41107
            1741-1767           BX322561.1         41877-41903
            1768-1899           BX322561.1         45943-46074
            1900-1962           BX322561.1         46529-46591
            1963-1989           BX322561.1         47500-47526
            1990-2025           BX322561.1         48568-48603
            2026-2097           BX322561.1         49357-49428
            2098-2133           BX322561.1         49918-49953
            2134-2223           BX322561.1         50307-50396
            2224-2253           BX322561.1         51617-51646
            2254-2280           BX322561.1         51769-51795
            2281-2343           BX322561.1         52245-52307
            2344-2418           BX322561.1         52583-52657
            2419-2445           BX322561.1         53627-53653
            2446-2499           BX322561.1         53944-53997
            2500-2574           BX322561.1         54440-54514
            2575-2643           BX322561.1         55582-55650
            2644-2686           BX322561.1         56132-56174
            2687-2749           BX322561.1         56681-56743
            2750-2793           BX322561.1         63093-63136
            2794-2934           BX322561.1         63498-63638
            2935-3079           BX322561.1         64216-64360
            3080-3153           BX322561.1         64440-64513
            3154-3282           BX322561.1         64920-65048
            3283-3315           BX322561.1         66643-66675
            3316-3561           BX322561.1         68438-68683
            3562-3759           BX322561.1         69146-69343
            3760-3875           BX322561.1         70193-70308
            3876-5408           BX322561.1         71270-72802
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="21"
                     /map="21q22.3"
     gene            1..5408
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /note="collagen type XVIII alpha 1 chain"
                     /db_xref="GeneID:80781"
                     /db_xref="HGNC:HGNC:2195"
                     /db_xref="MIM:120328"
     exon            1..77
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     CDS             67..4086
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /note="isoform 4 preproprotein is encoded by transcript
                     variant 4; endostatin; antiangiogenic agent;
                     multi-functional protein MFP; collagen, type XVIII, alpha
                     1"
                     /codon_start=1
                     /product="collagen alpha-1(XVIII) chain isoform 4
                     preproprotein"
                     /protein_id="NP_001366429.1"
                     /db_xref="CCDS:CCDS42971.1"
                     /db_xref="GeneID:80781"
                     /db_xref="HGNC:HGNC:2195"
                     /db_xref="MIM:120328"
                     /translation="MAPRCPWPWPRRRRLLDVLAPLVLLLGVRAASAEPERISEEVGL
                     LQLLGDPPPQQVTQTDDPDVGLAYVFGPDANSGQVARYHFPSLFFRDFSLLFHIRPAT
                     EGPGVLFAITDSAQAMVLLGVKLSGVQDGHQDISLLYTEPGAGQTHTAASFRLPAFVG
                     QWTHLALSVAGGFVALYVDCEEFQRMPLARSSRGLELEPGAGLFVAQAGGADPDKFQG
                     VIAELKVRRDPQVSPMHCLDEEGDDSDGASGDSGSGLGDARELLREETGAALKPRLPA
                     PPPVTTPPLAGGSSTEDSRSEEVEEQTTVASLGAQTLPGSDSVSTWDGSVRTPGGRVK
                     EGGLKGQKGEPGVPGPPGRAGPPGSPCLPGPPGLPCPVSPLGPAGPALQTVPGPQGPP
                     GPPGRDGTPGRDGEPGDPGEDGKPGDTGPQGFPGTPGDVGPKGDKGDPGVGERGPPGP
                     QGPPGPPGPSFRHDKLTFIDMEGSGFGGDLEALRGPRGFPGPPGPPGVPGLPGEPGRF
                     GVNSSDVPGPAGLPGVPGREGPPGFPGLPGPPGPPGREGPPGRTGQKGSLGEAGAPGH
                     KGSKGAPGPAGARGESGLAGAPGPAGPPGPPGPPGPPGPGLPAGFDDMEGSGGPFWST
                     ARSADGPQGPPGLPGLKGDPGVPGLPGAKGEVGADGVPGFPGLPGREGIAGPQGPKGD
                     RGSRGEKGDPGKDGVGQPGLPGPPGPPGPVVYVSEQDGSVLSVPGPEGRPGFAGFPGP
                     AGPKGNLGSKGERGSPGPKGEKGEPGSIFSPDGGALGPAQKGAKGEPGFRGPPGPYGR
                     PGYKGEIGFPGRPGRPGMNGLKGEKGEPGDASLGFGMRGMPGPPGPPGPPGPPGTPVY
                     DSNVFAESSRPGPPGLPGNQGPPGPKGAKGEVGPPGPPGQFPFDFLQLEAEMKGEKGD
                     RGDAGQKGERGEPGGGGFFGSSLPGPPGPPGPPGPRGYPGIPGPKGESIRGQPGPPGP
                     QGPPGIGYEGRQGPPGPPGPPGPPSFPGPHRQTISVPGPPGPPGPPGPPGTMGASSGV
                     RLWATRQAMLGQVHEVPEGWLIFVAEQEELYVRVQNGFRKVQLEARTPLPRGTDNEVA
                     ALQPPVVQLHDSNPYPRREHPHPTARPWRADDILASPPRLPEPQPYPGAPHHSSYVHL
                     RPARPTSPPAHSHRDFQPVLHLVALNSPLSGGMRGIRGADFQCFQQARAVGLAGTFRA
                     FLSSRLQDLYSIVRRADRAAVPIVNLKDELLFPSWEALFSGSEGPLKPGARIFSFDGK
                     DVLRHPTWPQKSVWHGSDPNGRRLTESYCETWRTEAPSATGQASSLLGGRLLGQSAAS
                     CHHAYIVLCIENSFMTASK"
     exon            78..172
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            173..717
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            718..804
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            805..864
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            865..994
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            995..1071
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            1072..1287
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            1288..1314
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            1315..1377
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            1378..1464
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            1465..1518
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            1519..1677
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            1678..1740
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            1741..1767
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            1768..1899
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            1900..1962
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            1963..1989
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            1990..2025
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            2026..2097
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            2098..2133
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            2134..2223
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            2224..2253
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            2254..2280
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            2281..2343
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            2344..2418
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            2419..2445
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            2446..2499
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            2500..2574
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            2575..2643
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            2644..2686
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            2687..2749
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            2750..2793
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            2794..2934
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            2935..3079
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            3080..3153
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            3154..3282
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            3283..3315
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            3316..3561
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            3562..3759
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            3760..3875
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     exon            3876..5408
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5385..5390
                     /regulatory_class="polyA_signal_sequence"
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /note="hexamer: AATAAA"
     polyA_site      5408
                     /gene="COL18A1"
                     /gene_synonym="GLCC; KNO; KNO1; KS"
                     /note="major polyA site"
ORIGIN      
        1 ggtccgagcg ggtgcaccgc ggcggaggag gcagcatccc gcggcgctga cggtcctggg
       61 gagagcatgg cgccgaggtg cccctggcca tggccgcggc ggcggcgcct cctggacgtg
      121 ctcgcgcccc tggtcctgct gctcggggtc cgcgcggcct ccgcggagcc agagcgcatc
      181 agcgaggagg tggggctgct gcagctcctt ggggaccccc cgccccagca ggtcacccag
      241 acggatgacc ccgacgtcgg gctggcctac gtctttgggc cagatgccaa cagtggccaa
      301 gtggcccggt accacttccc cagcctcttc ttccgtgact tctcactgct gttccacatc
      361 cggccagcca cagagggccc aggggtgctg ttcgccatca cggactcggc gcaggccatg
      421 gtcttgctgg gcgtgaagct ctctggggtg caggacgggc accaggacat ctccctgctc
      481 tacacagaac caggtgcagg ccagacccac acagccgcca gcttccggct ccccgccttc
      541 gtcggccagt ggacacactt agccctcagt gtggcaggtg gctttgtggc cctctacgtg
      601 gactgtgagg agttccagag aatgccgctt gctcggtcct cacggggcct ggagctggag
      661 cctggcgccg ggctcttcgt ggctcaggcg gggggagcgg accctgacaa gttccagggg
      721 gtgatcgctg agctgaaggt gcgcagggac ccccaggtga gccccatgca ctgcctggac
      781 gaggaaggcg atgactcaga tggggcatcc ggagactctg gcagcgggct cggggacgcc
      841 cgggagcttc tcagggagga gacgggcgcg gccctaaaac ccaggctccc cgcgccaccc
      901 cccgtcacca cgccaccctt ggctggaggc agcagcacgg aagattccag aagtgaagaa
      961 gtcgaggagc agaccacggt ggcttcgtta ggagctcaga cacttcctgg ctcagattct
     1021 gtctccacgt gggacgggag tgtccggacc cctgggggcc gcgtgaaaga gggcggcctg
     1081 aaggggcaga aaggggagcc aggtgttccg ggcccacctg gccgggcagg ccccccagga
     1141 tccccatgcc tacctggtcc cccgggtctc ccgtgcccag tgagtcccct gggtcctgca
     1201 ggcccagcgt tgcaaactgt ccccggacca caaggacccc cagggcctcc ggggagggac
     1261 ggcacccctg gaagggacgg cgagccgggc gaccccggtg aagacggaaa gccgggcgac
     1321 accgggccac aaggcttccc cgggactcca ggggacgtag gtcccaaggg cgacaaggga
     1381 gaccctgggg ttggagagag agggccccca ggaccccaag ggcctccagg gcccccagga
     1441 ccctccttca gacacgacaa gctgaccttc attgacatgg agggatctgg cttcgggggc
     1501 gatctggagg ccctgcgggg tcctcgaggc ttccctggac ctcccggacc ccccggtgtc
     1561 ccaggcctgc ccggcgagcc aggccgcttt ggggtgaaca gctccgacgt cccaggaccc
     1621 gccggccttc ctggtgtgcc tgggcgcgag ggtccccccg ggtttcctgg cctcccggga
     1681 cccccaggcc ctccgggaag agaggggccc ccaggaagga ctgggcagaa aggcagcctg
     1741 ggtgaagcag gcgccccagg acataagggg agcaagggag cccccggtcc tgctggtgct
     1801 cgtggggaga gcggcctggc aggagccccc ggacctgctg gaccaccagg cccccctggg
     1861 ccccctgggc ccccaggacc aggactcccc gctggatttg atgacatgga aggctccggg
     1921 gggcccttct ggtcaacagc ccgaagcgct gatgggccac agggacctcc cggcctgccg
     1981 ggacttaagg gggatcctgg cgtgcctggg ctgccggggg cgaagggaga agttggagca
     2041 gatggagtcc ccgggttccc cggcctccct ggcagagagg gcattgctgg gccccagggg
     2101 ccaaagggag acagaggcag ccggggagaa aagggagatc cagggaagga cggagtcggg
     2161 cagccgggcc tccctggccc ccccggaccc ccgggacctg tggtctacgt gtcggagcag
     2221 gacggatccg tcctgagcgt gccgggacct gagggccggc cgggtttcgc aggctttccc
     2281 ggacctgcag gacccaaggg caacctgggc tctaagggcg aacgaggctc cccgggaccc
     2341 aagggtgaga agggtgaacc gggcagcatc ttcagccccg acggcggtgc cctgggccct
     2401 gcccagaaag gagccaaggg agagccgggc ttccgaggac ccccgggtcc atacggacgg
     2461 ccggggtaca agggagagat tggctttcct ggacggccgg gtcgccccgg gatgaacgga
     2521 ttgaaaggag agaaagggga gccgggagat gccagccttg gatttggcat gaggggaatg
     2581 cccggccccc caggacctcc agggccccca ggccctccag ggactcctgt ttacgacagc
     2641 aatgtgtttg ctgagtccag ccgccccggg cctccaggat tgccagggaa tcagggccct
     2701 ccaggaccca agggcgccaa aggagaagtg ggcccccccg gaccaccagg gcagtttccg
     2761 tttgactttc ttcagttgga ggctgaaatg aagggggaga agggagaccg aggtgatgca
     2821 ggacagaaag gcgaaagggg ggagcccggg ggcggcggtt tcttcggctc cagcctgccc
     2881 ggcccccccg gccccccagg ccccccaggc ccacgtggct accctgggat tccaggtccc
     2941 aagggagaga gcatccgggg ccagcccggc ccacctggac ctcagggacc ccccggcatc
     3001 ggctacgagg ggcgccaggg ccctcccggc cccccaggcc ccccagggcc cccttcattt
     3061 cctggccctc acaggcagac tatcagcgtt cccggccctc cgggcccccc tgggccccct
     3121 gggccccctg gaaccatggg cgcctcctca ggggtgaggc tctgggctac acgccaggcc
     3181 atgctgggcc aggtgcacga ggttcccgag ggctggctca tcttcgtggc cgagcaggag
     3241 gagctctacg tccgcgtgca gaacgggttc cggaaggtcc agctggaggc ccggacacca
     3301 ctcccacgag ggacggacaa tgaagtggcc gccttgcagc cccccgtggt gcagctgcac
     3361 gacagcaacc cctacccgcg gcgggagcac ccccacccca ccgcgcggcc ctggcgggca
     3421 gatgacatcc tggccagccc ccctcgcctg cccgagcccc agccctaccc cggagccccg
     3481 caccacagct cctacgtgca cctgcggccg gcgcgaccca caagcccacc cgcccacagc
     3541 caccgcgact tccagccggt gctccacctg gttgcgctca acagccccct gtcaggcggc
     3601 atgcggggca tccgcggggc cgacttccag tgcttccagc aggcgcgggc cgtggggctg
     3661 gcgggcacct tccgcgcctt cctgtcctcg cgcctgcagg acctgtacag catcgtgcgc
     3721 cgtgccgacc gcgcagccgt gcccatcgtc aacctcaagg acgagctgct gtttcccagc
     3781 tgggaggctc tgttctcagg ctctgagggt ccgctgaagc ccggggcacg catcttctcc
     3841 tttgacggca aggacgtcct gaggcacccc acctggcccc agaagagcgt gtggcatggc
     3901 tcggacccca acgggcgcag gctgaccgag agctactgtg agacgtggcg gacggaggct
     3961 ccctcggcca cgggccaggc ctcctcgctg ctggggggca ggctcctggg gcagagtgcc
     4021 gcgagctgcc atcacgccta catcgtgctc tgcattgaga acagcttcat gactgcctcc
     4081 aagtagccac cgcctggatg cggatggccg gagaggaccg gcggctcgga ggaagccccc
     4141 accgtgggca gggagcggcc ggccagcccc tggccccagg acctggctgc catactttcc
     4201 tgtatagttc acgtttcatg taatcctcaa gaaataaaag gaagccaaag agtgtatttt
     4261 tttaaaagtt taaaacagaa gcctgatgct gacattcacc tgccccaact ctcccctgac
     4321 ctgtgagccc agctgggtca ggcagggtgc agtatcatgc cctgtgcaac ctcttggcct
     4381 gatcagacca cggctcgatt tctccaggat ttcctgcttt gggaagccgt gctcgcccca
     4441 gcaggtgctg acttcatctc ccacctagca gcaccgttct gtgcacaaaa cccagacctg
     4501 ttagcagaca ggccccgtga ggcaatggga gctgaggcca cactcagcac aaggccatct
     4561 gggctcctcc agggtgtgtg ctcgccctgc ggtagatggg agggaggctc aggtccctgg
     4621 ggctaggggg agccccttct gctcagctct gggccattct ccacagcaac cccaggctga
     4681 agcaggttcc caagctcaga ggcgcactgt gacccccagc tccggcctgt cctccaacac
     4741 caagcacagc agcctggggc tggcctccca aatgagccat gagatgatac atccaaagca
     4801 gacagctcca ccctggccga gtccaagctg ggagattcaa gggacccatg agttggggtc
     4861 tggcagcctc ccatccaggg cccccatctc atgcccctgg ctgggacgtg gctcagccag
     4921 cacttgtcca gctgagcgcc aggatggaac acggccacat caaagaggct gaggctggca
     4981 caggacatgc ggtagccagc acacagggca gtgagggagg gctgtcatct gtgcactgcc
     5041 catggacagg ctggctccag atgcagggca gtcattggct gtctcctagg aaacccatat
     5101 ccttaccctc cttgggactg aaggggaacc ccggggtgcc cacaggccgc cctgcgggtg
     5161 aacaaagcag ccacgaggtg caacaaggtc ctctgtcagt cacagccacc cctgagatcc
     5221 ggcaacatca acccgagtca ttcgttctgt ggagggacaa gtggactcag ggcagcgcca
     5281 ggctgaccac agcacagcca acacgcacct gcctcaggac tgcgacgaaa ccggtggggc
     5341 tggttctgta attgtgtgtg atgtgaagcc aattcagaca ggcaaataaa agtgaccttt
     5401 tacactga
//
