LOCUS       NM_014255               1450 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens canopy FGF signaling regulator 2 (CNPY2), transcript
            variant 1, mRNA.
ACCESSION   NM_014255
VERSION     NM_014255.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Tian,T., Dong,Y., Zhu,Y., Chen,Y., Li,X., Kuang,Q., Liu,X., Li,P.,
            Li,J. and Zhou,L.
  TITLE     Hypoxia-induced CNPY2 upregulation promotes glycolysis in cervical
            cancer through activation of AKT pathway
  JOURNAL   Biochem Biophys Res Commun 551, 63-70 (2021)
   PUBMED   33721832
  REMARK    GeneRIF: Hypoxia-induced CNPY2 upregulation promotes glycolysis in
            cervical cancer through activation of AKT pathway.
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Lo,L.H., Lam,C.Y., To,J.C., Chiu,C.H. and Keng,V.W.
  TITLE     Sleeping Beauty insertional mutagenesis screen identifies the
            pro-metastatic roles of CNPY2 and ACTN2 in hepatocellular carcinoma
            tumor progression
  JOURNAL   Biochem Biophys Res Commun 541, 70-77 (2021)
   PUBMED   33482578
  REMARK    GeneRIF: Sleeping Beauty insertional mutagenesis screen identifies
            the pro-metastatic roles of CNPY2 and ACTN2 in hepatocellular
            carcinoma tumor progression.
REFERENCE   3  (bases 1 to 1450)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 1450)
  AUTHORS   Wang,H., Kanmangne,D., Li,R., Qian,Z., Xia,X., Wang,X. and Wang,T.
  TITLE     miR-30a-3p suppresses the proliferation and migration of lung
            adenocarcinoma cells by downregulating CNPY2
  JOURNAL   Oncol Rep 43 (2), 646-654 (2020)
   PUBMED   31894275
  REMARK    GeneRIF: miR30a3p suppresses the proliferation and migration of
            lung adenocarcinoma cells by downregulating CNPY2.
REFERENCE   5  (bases 1 to 1450)
  AUTHORS   Yin,W., Guo,J., Zhang,C., Alibhai,F.J., Li,S.H., Billia,P., Wu,J.,
            Yau,T.M., Weisel,R.D. and Li,R.K.
  TITLE     Knockout of Canopy 2 activates p16INK4a pathway to impair cardiac
            repair
  JOURNAL   J Mol Cell Cardiol 132, 36-48 (2019)
   PUBMED   31047986
  REMARK    GeneRIF: Knockout of Cnpy2 resulted in up-regulation of p16(INK4a).
REFERENCE   6  (bases 1 to 1450)
  AUTHORS   Trynka,G., Zhernakova,A., Romanos,J., Franke,L., Hunt,K.A.,
            Turner,G., Bruinenberg,M., Heap,G.A., Platteel,M., Ryan,A.W., de
            Kovel,C., Holmes,G.K., Howdle,P.D., Walters,J.R., Sanders,D.S.,
            Mulder,C.J., Mearin,M.L., Verbeek,W.H., Trimble,V., Stevens,F.M.,
            Kelleher,D., Barisani,D., Bardella,M.T., McManus,R., van Heel,D.A.
            and Wijmenga,C.
  TITLE     Coeliac disease-associated risk variants in TNFAIP3 and REL
            implicate altered NF-kappaB signalling
  JOURNAL   Gut 58 (8), 1078-1083 (2009)
   PUBMED   19240061
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 1450)
  AUTHORS   Bornhauser,B.C. and Lindholm,D.
  TITLE     MSAP enhances migration of C6 glioma cells through phosphorylation
            of the myosin regulatory light chain
  JOURNAL   Cell Mol Life Sci 62 (11), 1260-1266 (2005)
   PUBMED   15905959
REFERENCE   8  (bases 1 to 1450)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   9  (bases 1 to 1450)
  AUTHORS   Bornhauser,B.C., Olsson,P.A. and Lindholm,D.
  TITLE     MSAP is a novel MIR-interacting protein that enhances neurite
            outgrowth and increases myosin regulatory light chain
  JOURNAL   J Biol Chem 278 (37), 35412-35420 (2003)
   PUBMED   12826659
  REMARK    GeneRIF: MSAP is a novel MIR-interacting protein that enhances
            neurite outgrowth and increases myosin regulatory light chain in
            fetal and adult brain.
REFERENCE   10 (bases 1 to 1450)
  AUTHORS   Yokoyama-Kobayashi,M., Yamaguchi,T., Sekine,S. and Kato,S.
  TITLE     Selection of cDNAs encoding putative type II membrane proteins on
            the cell surface from a human full-length cDNA bank
  JOURNAL   Gene 228 (1-2), 161-167 (1999)
   PUBMED   10072769
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AY359102.1, BE699270.1, BE858097.1 and AW293214.1.
            
            On Nov 23, 2018 this sequence version replaced NM_014255.6.
            
            Transcript Variant: This variant (1) is the longer transcript and
            encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.1106645.1,
                                           SRR1660803.59039.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000273308.9/ ENSP00000273308.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-861               AY359102.1         350-1210
            862-926             BE699270.1         156-220
            927-1137            BE858097.1         317-527             c
            1138-1450           AW293214.1         20-332              c
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13.3"
     gene            1..1450
                     /gene="CNPY2"
                     /gene_synonym="HP10390; MSAP; TMEM4; ZSIG9"
                     /note="canopy FGF signaling regulator 2"
                     /db_xref="GeneID:10330"
                     /db_xref="HGNC:HGNC:13529"
                     /db_xref="MIM:605861"
     exon            1..168
                     /gene="CNPY2"
                     /gene_synonym="HP10390; MSAP; TMEM4; ZSIG9"
                     /inference="alignment:Splign:2.1.0"
     exon            169..281
                     /gene="CNPY2"
                     /gene_synonym="HP10390; MSAP; TMEM4; ZSIG9"
                     /inference="alignment:Splign:2.1.0"
     CDS             194..742
                     /gene="CNPY2"
                     /gene_synonym="HP10390; MSAP; TMEM4; ZSIG9"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; MIR-interacting saposin-like protein;
                     transmembrane protein 4; protein canopy homolog 2;
                     putative secreted protein Zsig9"
                     /codon_start=1
                     /product="protein canopy homolog 2 isoform 1 precursor"
                     /protein_id="NP_055070.1"
                     /db_xref="CCDS:CCDS8914.1"
                     /db_xref="GeneID:10330"
                     /db_xref="HGNC:HGNC:13529"
                     /db_xref="MIM:605861"
                     /translation="MKGWGWLALLLGALLGTAWARRSQDLHCGACRALVDELEWEIAQ
                     VDPKKTIQMGSFRINPDGSQSVVEVPYARSEAHLTELLEEICDRMKEYGEQIDPSTHR
                     KNYVRVVGRNGESSELDLQGIRIDSDISGTLKFACESIVEEYEDELIEFFSREADNVK
                     DKLCSKRTDLCDHALHISHDEL"
     exon            282..397
                     /gene="CNPY2"
                     /gene_synonym="HP10390; MSAP; TMEM4; ZSIG9"
                     /inference="alignment:Splign:2.1.0"
     exon            398..601
                     /gene="CNPY2"
                     /gene_synonym="HP10390; MSAP; TMEM4; ZSIG9"
                     /inference="alignment:Splign:2.1.0"
     exon            602..698
                     /gene="CNPY2"
                     /gene_synonym="HP10390; MSAP; TMEM4; ZSIG9"
                     /inference="alignment:Splign:2.1.0"
     exon            699..1450
                     /gene="CNPY2"
                     /gene_synonym="HP10390; MSAP; TMEM4; ZSIG9"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      862
                     /gene="CNPY2"
                     /gene_synonym="HP10390; MSAP; TMEM4; ZSIG9"
                     /note="major polyA site"
     regulatory      1430..1435
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CNPY2"
                     /gene_synonym="HP10390; MSAP; TMEM4; ZSIG9"
                     /note="hexamer: AATAAA"
     polyA_site      1450
                     /gene="CNPY2"
                     /gene_synonym="HP10390; MSAP; TMEM4; ZSIG9"
ORIGIN      
        1 aggcggtcgc cgggacaccc cgtgtgtggc aggcggcgaa gcgctctgga gaatcccgga
       61 cagccctgct ccctgcagcc aggtgtagtt tcgggagcca ctggggccaa agtgagagtc
      121 cagcggtctt ccagcgcttg ggccacggcg gcggccctgg gagcagaggt ggagcgaccc
      181 cattacgcta aagatgaaag gctggggttg gctggccctg cttctggggg ccctgctggg
      241 aaccgcctgg gctcggagga gccaggatct ccactgtgga gcatgcaggg ctctggtgga
      301 tgaactagaa tgggaaattg cccaggtgga ccccaagaag accattcaga tgggatcttt
      361 ccggatcaat ccagatggca gccagtcagt ggtggaggtg ccttatgccc gctcagaggc
      421 ccacctcaca gagctgctgg aggagatatg tgaccggatg aaggagtatg gggaacagat
      481 tgatccttcc acccatcgca agaactacgt acgtgtagtg ggccggaatg gagaatccag
      541 tgaactggac ctacaaggca tccgaatcga ctcagatatt agcggcaccc tcaagtttgc
      601 gtgtgagagc attgtggagg aatacgagga tgaactcatt gaattctttt cccgagaggc
      661 tgacaatgtt aaagacaaac tttgcagtaa gcgaacagat ctttgtgacc atgccctgca
      721 catatcgcat gatgagctat gaaccactgg agcagcccac actggcttga tggatcaccc
      781 ccaggagggg aaaatggtgg caatgccttt tatatattat gtttttactg aaattaactg
      841 aaaaaatatg aaaccaaaag tacaaactgt gttgtctttt catgcctgga attgccctta
      901 cattaagctt gaagataagg agttatatag acaggaaaga taatggagga gaaagaagag
      961 ttttgggatt gatacaggat aaaggtagga gaggcctgaa cctttaattc ttctaccttc
     1021 tgagattcca ctccactcat aaactagata agctttcagt cagtgcccac tcaataccac
     1081 gtctttagag tctaattgtg gagaatagag gaacctccat gaggaagcag gggaacatag
     1141 tgtatggtag tgtagaattc tagaaatccc ttgtgttcat ggctgttgaa acataaagta
     1201 agacagggct tggtcttgaa ggaaataacg gatatgcatt attcttcctt ccctgcccat
     1261 ccagatggag ggaccagcaa gaacagagac aggaagggga aaatggatgt gaccaactca
     1321 gctgaaaagt aagggcttta tcggataggg acagacgagg taaggaaggt tgtttaatga
     1381 gaggccttgc ttgccagcct aagcttttaa atttgatgcc agtggcaaca ataaagtgta
     1441 tctatcacta
//
