LOCUS       NM_001301073            2446 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens CCR4-NOT transcription complex subunit 8 (CNOT8),
            transcript variant 2, mRNA.
ACCESSION   NM_001301073 XM_005268526
VERSION     NM_001301073.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Almasmoum,H.A., Airhihen,B., Seedhouse,C. and Winkler,G.S.
  TITLE     Frequent loss of BTG1 activity and impaired interactions with the
            Caf1 subunit of the Ccr4-Not deadenylase in non-Hodgkin lymphoma
  JOURNAL   Leuk Lymphoma 62 (2), 281-290 (2021)
   PUBMED   33021411
  REMARK    GeneRIF: Frequent loss of BTG1 activity and impaired interactions
            with the Caf1 subunit of the Ccr4-Not deadenylase in non-Hodgkin
            lymphoma.
REFERENCE   3  (bases 1 to 2446)
  AUTHORS   Zeng,X., Li,C., Li,Y., Yu,H., Fu,P., Hong,H.G. and Zhang,W.
  TITLE     A network-based variable selection approach for identification of
            modules and biomarker genes associated with end-stage kidney
            disease
  JOURNAL   Nephrology (Carlton) 25 (10), 775-784 (2020)
   PUBMED   31464346
  REMARK    GeneRIF: A network-based variable selection approach for
            identification of modules and biomarker genes associated with
            end-stage kidney disease.
REFERENCE   4  (bases 1 to 2446)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 2446)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2446)
  AUTHORS   Prevot,D., Morel,A.P., Voeltzel,T., Rostan,M.C., Rimokh,R.,
            Magaud,J.P. and Corbo,L.
  TITLE     Relationships of the antiproliferative proteins BTG1 and BTG2 with
            CAF1, the human homolog of a component of the yeast CCR4
            transcriptional complex: involvement in estrogen receptor alpha
            signaling pathway
  JOURNAL   J Biol Chem 276 (13), 9640-9648 (2001)
   PUBMED   11136725
REFERENCE   7  (bases 1 to 2446)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
REFERENCE   8  (bases 1 to 2446)
  AUTHORS   Albert,T.K., Lemaire,M., van Berkum,N.L., Gentz,R., Collart,M.A.
            and Timmers,H.T.
  TITLE     Isolation and characterization of human orthologs of yeast CCR4-NOT
            complex subunits
  JOURNAL   Nucleic Acids Res 28 (3), 809-817 (2000)
   PUBMED   10637334
REFERENCE   9  (bases 1 to 2446)
  AUTHORS   Fidler,C., Wainscoat,J.S. and Boultwood,J.
  TITLE     The human POP2 gene: identification, sequencing, and mapping to the
            critical region of the 5q- syndrome
  JOURNAL   Genomics 56 (1), 134-136 (1999)
   PUBMED   10036195
REFERENCE   10 (bases 1 to 2446)
  AUTHORS   Bogdan,J.A., Adams-Burton,C., Pedicord,D.L., Sukovich,D.A.,
            Benfield,P.A., Corjay,M.H., Stoltenborg,J.K. and Dicker,I.B.
  TITLE     Human carbon catabolite repressor protein (CCR4)-associative factor
            1: cloning, expression and characterization of its interaction with
            the B-cell translocation protein BTG1
  JOURNAL   Biochem J 336 (Pt 2) (Pt 2), 471-481 (1998)
   PUBMED   9820826
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK025358.1 and AL122045.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001301073.1.
            
            Transcript Variant: This variant (2) differs in the 5' UTR and
            encode the same isoform (1), compared to variant 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK025358.1, BC017366.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000285896.11/ ENSP00000285896.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-668               AK025358.1         78-745
            669-2446            AL122045.1         706-2483
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q33.2"
     gene            1..2446
                     /gene="CNOT8"
                     /gene_synonym="CAF1; Caf1b; CALIF; hCAF1; POP2"
                     /note="CCR4-NOT transcription complex subunit 8"
                     /db_xref="GeneID:9337"
                     /db_xref="HGNC:HGNC:9207"
                     /db_xref="MIM:603731"
     exon            1..142
                     /gene="CNOT8"
                     /gene_synonym="CAF1; Caf1b; CALIF; hCAF1; POP2"
                     /inference="alignment:Splign:2.1.0"
     exon            143..331
                     /gene="CNOT8"
                     /gene_synonym="CAF1; Caf1b; CALIF; hCAF1; POP2"
                     /inference="alignment:Splign:2.1.0"
     CDS             215..1093
                     /gene="CNOT8"
                     /gene_synonym="CAF1; Caf1b; CALIF; hCAF1; POP2"
                     /EC_number="3.1.13.4"
                     /note="isoform 1 is encoded by transcript variant 2; PGK
                     promoter directed over production; CAF2; CALIFp; CAF1-like
                     protein; CCR4-associated factor 8"
                     /codon_start=1
                     /product="CCR4-NOT transcription complex subunit 8 isoform
                     1"
                     /protein_id="NP_001288002.1"
                     /db_xref="CCDS:CCDS4329.1"
                     /db_xref="GeneID:9337"
                     /db_xref="HGNC:HGNC:9207"
                     /db_xref="MIM:603731"
                     /translation="MPAALVENSQVICEVWASNLEEEMRKIREIVLSYSYIAMDTEFP
                     GVVVRPIGEFRSSIDYQYQLLRCNVDLLKIIQLGLTFTNEKGEYPSGINTWQFNFKFN
                     LTEDMYSQDSIDLLANSGLQFQKHEEEGIDTLHFAELLMTSGVVLCDNVKWLSFHSGY
                     DFGYMVKLLTDSRLPEEEHEFFHILNLFFPSIYDVKYLMKSCKNLKGGLQEVADQLDL
                     QRIGRQHQAGSDSLLTGMAFFRMKELFFEDSIDDAKYCGRLYGLGTGVAQKQNEDVDS
                     AQEKMSILAIINNMQQ"
     exon            332..525
                     /gene="CNOT8"
                     /gene_synonym="CAF1; Caf1b; CALIF; hCAF1; POP2"
                     /inference="alignment:Splign:2.1.0"
     exon            526..687
                     /gene="CNOT8"
                     /gene_synonym="CAF1; Caf1b; CALIF; hCAF1; POP2"
                     /inference="alignment:Splign:2.1.0"
     exon            688..832
                     /gene="CNOT8"
                     /gene_synonym="CAF1; Caf1b; CALIF; hCAF1; POP2"
                     /inference="alignment:Splign:2.1.0"
     exon            833..943
                     /gene="CNOT8"
                     /gene_synonym="CAF1; Caf1b; CALIF; hCAF1; POP2"
                     /inference="alignment:Splign:2.1.0"
     exon            944..2446
                     /gene="CNOT8"
                     /gene_synonym="CAF1; Caf1b; CALIF; hCAF1; POP2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1413..1418
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CNOT8"
                     /gene_synonym="CAF1; Caf1b; CALIF; hCAF1; POP2"
                     /note="hexamer: AGTAAA"
     polyA_site      1434
                     /gene="CNOT8"
                     /gene_synonym="CAF1; Caf1b; CALIF; hCAF1; POP2"
     regulatory      2419..2424
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CNOT8"
                     /gene_synonym="CAF1; Caf1b; CALIF; hCAF1; POP2"
                     /note="hexamer: AATAAA"
     polyA_site      2446
                     /gene="CNOT8"
                     /gene_synonym="CAF1; Caf1b; CALIF; hCAF1; POP2"
                     /note="major polyA site"
ORIGIN      
        1 gttcggttct aggagcagat ccggggtaga gggaaaagag ctccgggcca ggggctgccg
       61 tcgccgccgt cggggagtca gcccgccagc ccgccagctc gtcagcccgc cagccagcgc
      121 ttcgcgggcc ctgtcggtcc cgatcagacc aaacattgtc tggcttgcac tgtaaaacta
      181 gttagctgaa gacgacttct caggtttctt caggatgcct gcagcacttg tggagaatag
      241 ccaggttatc tgtgaagtgt gggccagtaa tctagaagaa gagatgagga agatccgaga
      301 aatcgtgctc agttacagtt atattgccat ggacacagaa tttccaggtg ttgtggtgcg
      361 accaattggt gaatttcgta gttccataga ttaccaatat cagcttctgc ggtgcaatgt
      421 tgacctttta aaaattatcc agctgggcct tacattcaca aatgagaagg gagagtatcc
      481 ttctggaatc aatacttggc agttcaattt caaatttaac cttacagagg acatgtactc
      541 ccaggattcc atagatctcc ttgctaactc aggactacag tttcagaagc atgaagagga
      601 agggattgac acactgcact ttgcagagct gcttatgaca tcaggagtgg ttctctgtga
      661 caatgtcaaa tggctttcat ttcatagtgg ctatgatttt ggctatatgg taaagttgct
      721 tacagattct cgtttgccag aagaggaaca tgaattcttt catattctga accttttctt
      781 cccatccatt tatgatgtga aatacctgat gaagagctgc aaaaatctta agggaggtct
      841 tcaggaagtt gctgatcagt tggatttgca gaggattgga aggcagcacc aggcaggctc
      901 agactcactg ctgacaggaa tggctttctt taggatgaaa gagttgtttt ttgaggacag
      961 cattgatgat gccaagtact gtgggcggct ctatggctta ggcacaggag tggcccagaa
     1021 gcagaatgag gatgtggact ctgcccagga gaagatgagc atcctggcga ttatcaacaa
     1081 catgcagcag tgatggcgcc aggctctgca gggtgggcct gatcccagag tggtgcttac
     1141 tgtgctgact gtgtacttat cttccccaag agaaaatgct tcttttgagc acactgtacc
     1201 taccatctgc attgagcaga aagacttttg ttttactgaa gacaaaagat gtttttattt
     1261 tagacccaga agagaggagt ttgctctgaa tttgtaaata agtcttcccc attcctcata
     1321 ctcgagcctc tcctctctgg ttgcctcctg ccaccagcat ccatggctca tttgacacct
     1381 ttttaaatat caggacaagt ctgaaacaaa gtagtaaaat gtatataact cttacctgtt
     1441 gtcattcttt ttcttttaaa tttgttgcta atctctgata atgaagattc ttactctgat
     1501 tctcagctga gctgtgaggg cttccaggga aaatggaaca aaatggtgtt cttaggtaat
     1561 gggttgtaga tactgagtct tcctttcctt ttctgaccct tctcgaggac atttgctttc
     1621 ctcacacttt tgtagtctct ctttacatat tactatatgg aaatgaattg ctctgtgctg
     1681 aaatttgaag accagataat gaaactgaaa agcaaacaat tttactgaat ctgtctaccc
     1741 ttcattcatg agaactccag aatgagtgtt gaccactgaa gcatctttta agtctgtgtt
     1801 ccattgtgcc attcaggttt gctgtcacat atgcatcatc tgaaatcatt tgaaattttt
     1861 gtacaataaa atatcctgga tttgatcctg aaggaaacta gtaagatcag atttttgggt
     1921 catgtctgtt gtattttcag taatgtgatt tcagatggtc atctggattc tcccacttct
     1981 ctactccatt atttctctac ttttccttcc agcaaacctg aaacgtgagg gagatggatt
     2041 aatgtgagta acaggaatgt gtctttaaaa agctagagtg gttacattta atcaggcagt
     2101 aagataattt gggttcttga gttgttttgg agtaatatcc cacaactggg gtaggaagct
     2161 caggactttt ttctttaaag ctagtcattt caaaagcata ttgtattttt ttgaatgact
     2221 acagtatgga caatttcaaa aaccaaaacc cactttggat tggtggaagt aaaaactggt
     2281 aactcactca agtgaatgaa tggtcttgca ttttaaaagc ttatgggaaa ctcaatttga
     2341 aatgattaga aaatgtcaag tattataagc tggtatttaa gatgcttgta aatactattt
     2401 atgtttttaa ttttgtaaaa taaagatttc tttttaacca ctggca
//
