LOCUS       NM_001190850            3538 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens CCR4-NOT transcription complex subunit 4 (CNOT4),
            transcript variant 6, mRNA.
ACCESSION   NM_001190850
VERSION     NM_001190850.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Zhang,B., Zhao,B., Han,S. and Chen,S.
  TITLE     CNOT4 suppresses nonsmall cell lung cancer progression by promoting
            the degradation of PAF1
  JOURNAL   Mol Carcinog 62 (10), 1563-1571 (2023)
   PUBMED   37493105
  REMARK    GeneRIF: CNOT4 suppresses nonsmall cell lung cancer progression by
            promoting the degradation of PAF1.
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Tsuda,M., Noguchi,M., Kurai,T., Ichihashi,Y., Ise,K., Wang,L.,
            Ishida,Y., Tanino,M., Hirano,S., Asaka,M. and Tanaka,S.
  TITLE     Aberrant expression of MYD88 via RNA-controlling CNOT4 and EXOSC3
            in colonic mucosa impacts generation of colonic cancer
  JOURNAL   Cancer Sci 112 (12), 5100-5113 (2021)
   PUBMED   34626022
  REMARK    GeneRIF: Aberrant expression of MYD88 via RNA-controlling CNOT4 and
            EXOSC3 in colonic mucosa impacts generation of colonic cancer.
REFERENCE   3  (bases 1 to 3538)
  AUTHORS   Zhang,B. and Chen,S.
  TITLE     CNOT4 suppresses non-small cell lung cancer progression and is
            required for effector cytolytic T lymphocytes cell responses to
            lung cancer cells
  JOURNAL   Mol Immunol 132, 165-171 (2021)
   PUBMED   33592572
  REMARK    GeneRIF: CNOT4 suppresses non-small cell lung cancer progression
            and is required for effector cytolytic T lymphocytes cell responses
            to lung cancer cells.
REFERENCE   4  (bases 1 to 3538)
  AUTHORS   Beretti,F., Farnetani,F., Reggiani Bonetti,L., Fabbiani,L.,
            Zavatti,M., Maiorana,A., Pellacani,G. and Maraldi,T.
  TITLE     The Interplay between HGF/c-met Axis and Nox4 in BRAF Mutated
            Melanoma
  JOURNAL   Int J Mol Sci 22 (2), 761 (2021)
   PUBMED   33451139
  REMARK    GeneRIF: The Interplay between HGF/c-met Axis and Nox4 in BRAF
            Mutated Melanoma.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3538)
  AUTHORS   Zhang,B., Han,S., Ma,H. and Chen,S.
  TITLE     CNOT4 enhances the efficacy of anti-PD-1 immunotherapy in a model
            of non-small cell lung cancer
  JOURNAL   FEBS Open Bio 10 (12), 2631-2639 (2020)
   PUBMED   33034149
  REMARK    GeneRIF: CNOT4 enhances the efficacy of anti-PD-1 immunotherapy in
            a model of non-small cell lung cancer.
REFERENCE   6  (bases 1 to 3538)
  AUTHORS   Winkler,G.S., Albert,T.K., Dominguez,C., Legtenberg,Y.I.,
            Boelens,R. and Timmers,H.T.
  TITLE     An altered-specificity ubiquitin-conjugating
            enzyme/ubiquitin-protein ligase pair
  JOURNAL   J Mol Biol 337 (1), 157-165 (2004)
   PUBMED   15001359
  REMARK    GeneRIF: Data show that binding of the CNOT4 RING finger to the
            ubiquitin-conjugating enzyme (E2) UbcH5B is highly selective
REFERENCE   7  (bases 1 to 3538)
  AUTHORS   Wistow,G., Bernstein,S.L., Wyatt,M.K., Fariss,R.N., Behal,A.,
            Touchman,J.W., Bouffard,G., Smith,D. and Peterson,K.
  TITLE     Expressed sequence tag analysis of human RPE/choroid for the
            NEIBank Project: over 6000 non-redundant transcripts, novel genes
            and splice variants
  JOURNAL   Mol Vis 8, 205-220 (2002)
   PUBMED   12107410
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 3538)
  AUTHORS   Albert,T.K., Hanzawa,H., Legtenberg,Y.I., de Ruwe,M.J., van den
            Heuvel,F.A., Collart,M.A., Boelens,R. and Timmers,H.T.
  TITLE     Identification of a ubiquitin-protein ligase subunit within the
            CCR4-NOT transcription repressor complex
  JOURNAL   EMBO J 21 (3), 355-364 (2002)
   PUBMED   11823428
REFERENCE   9  (bases 1 to 3538)
  AUTHORS   Hanzawa,H., de Ruwe,M.J., Albert,T.K., van Der Vliet,P.C.,
            Timmers,H.T. and Boelens,R.
  TITLE     The structure of the C4C4 ring finger of human NOT4 reveals
            features distinct from those of C3HC4 RING fingers
  JOURNAL   J Biol Chem 276 (13), 10185-10190 (2001)
   PUBMED   11087754
REFERENCE   10 (bases 1 to 3538)
  AUTHORS   Albert,T.K., Lemaire,M., van Berkum,N.L., Gentz,R., Collart,M.A.
            and Timmers,H.T.
  TITLE     Isolation and characterization of human orthologs of yeast CCR4-NOT
            complex subunits
  JOURNAL   Nucleic Acids Res 28 (3), 809-817 (2000)
   PUBMED   10637334
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA247976.1, AK314540.1,
            AC083871.2, U71267.1, CA396599.1 and AC009331.6.
            
            On Aug 13, 2020 this sequence version replaced NM_001190850.1.
            
            Summary: The protein encoded by this gene is a subunit of the
            CCR4-NOT complex, a global transcriptional regulator. The encoded
            protein interacts with CNOT1 and has E3 ubiquitin ligase activity.
            Several transcript variants encoding different isoforms have been
            found for this gene.[provided by RefSeq, Jul 2010].
            
            Transcript Variant: This variant (6) encodes the longest isoform
            (f).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853564.25208.1,
                                           SRR11853558.22550.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000541284.6/ ENSP00000445508.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-167               DA247976.1         26-192
            168-277             AK314540.1         158-267
            278-278             AC083871.2         33742-33742         c
            279-1118            AK314540.1         269-1108
            1119-1367           U71267.1           1094-1342
            1368-1933           AK314540.1         1349-1914
            1934-2293           CA396599.1         220-579
            2294-3538           AC009331.6         144486-145730
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q33"
     gene            1..3538
                     /gene="CNOT4"
                     /gene_synonym="CLONE243; NOT4; NOT4H"
                     /note="CCR4-NOT transcription complex subunit 4"
                     /db_xref="GeneID:4850"
                     /db_xref="HGNC:HGNC:7880"
                     /db_xref="MIM:604911"
     exon            1..214
                     /gene="CNOT4"
                     /gene_synonym="CLONE243; NOT4; NOT4H"
                     /inference="alignment:Splign:2.1.0"
     exon            215..480
                     /gene="CNOT4"
                     /gene_synonym="CLONE243; NOT4; NOT4H"
                     /inference="alignment:Splign:2.1.0"
     CDS             307..2448
                     /gene="CNOT4"
                     /gene_synonym="CLONE243; NOT4; NOT4H"
                     /EC_number="2.3.2.27"
                     /note="isoform f is encoded by transcript variant 6; NOT4
                     (negative regulator of transcription 4, yeast) homolog;
                     CCR4-associated factor 4; E3 ubiquitin-protein ligase
                     CNOT4; potential transcriptional repressor NOT4Hp;
                     RING-type E3 ubiquitin transferase CNOT4"
                     /codon_start=1
                     /product="CCR4-NOT transcription complex subunit 4 isoform
                     f"
                     /protein_id="NP_001177779.1"
                     /db_xref="CCDS:CCDS55165.1"
                     /db_xref="GeneID:4850"
                     /db_xref="HGNC:HGNC:7880"
                     /db_xref="MIM:604911"
                     /translation="MSRSPDAKEDPVECPLCMEPLEIDDINFFPCTCGYQICRFCWHR
                     IRTDENGLCPACRKPYPEDPAVYKPLSQEELQRIKNEKKQKQNERKQKISENRKHLAS
                     VRVVQKNLVFVVGLSQRLADPEVLKRPEYFGKFGKIHKVVINNSTSYAGSQGPSASAY
                     VTYIRSEDALRAIQCVNNVVVDGRTLKASLGTTKYCSYFLKNMQCPKPDCMYLHELGD
                     EAASFTKEEMQAGKHQEYEQKLLQELYKLNPNFLQLSTGSVDKNKNKVTPLQRYDTPI
                     DKPSDSLSIGNGDNSQQISNSDTPSPPPGLSKSNPVIPISSSNHSARSPFEGAVTESQ
                     SLFSDNFRHPNPIPSGLPPFPSSPQTSSDWPTAPEPQSLFTSETIPVSSSTDWQAAFG
                     FGSSKQPEDDLGFDPFDVTRKALADLIEKELSVQDQPSLSPTSLQNSSSHTTTAKGPG
                     SGFLHPAAATNANSLNSTFSVLPQRFPQFQQHRAVYNSFSFPGQAARYPWMAFPRNSI
                     MHLNHTANPTSNSNFLDLNLPPQHNTGLGGIPVADNSSSVESLNMKEWQDGLRALLPN
                     ININFGGLPNSSSPSNANHSAPTSNTATTDSLSWDSPGSWTDPAIITGIPASSGNSLD
                     SLQDDNPPHWLKSLQALTEMDGPSAAPSQTHHSAPFSTQIPLHRASWNPYPPPSNPSS
                     FHSPPPGFQTAFRPPSKTPTDLLQSSTLDRH"
     exon            481..678
                     /gene="CNOT4"
                     /gene_synonym="CLONE243; NOT4; NOT4H"
                     /inference="alignment:Splign:2.1.0"
     exon            679..765
                     /gene="CNOT4"
                     /gene_synonym="CLONE243; NOT4; NOT4H"
                     /inference="alignment:Splign:2.1.0"
     exon            766..867
                     /gene="CNOT4"
                     /gene_synonym="CLONE243; NOT4; NOT4H"
                     /inference="alignment:Splign:2.1.0"
     exon            868..993
                     /gene="CNOT4"
                     /gene_synonym="CLONE243; NOT4; NOT4H"
                     /inference="alignment:Splign:2.1.0"
     exon            994..1127
                     /gene="CNOT4"
                     /gene_synonym="CLONE243; NOT4; NOT4H"
                     /inference="alignment:Splign:2.1.0"
     exon            1128..1185
                     /gene="CNOT4"
                     /gene_synonym="CLONE243; NOT4; NOT4H"
                     /inference="alignment:Splign:2.1.0"
     exon            1186..1435
                     /gene="CNOT4"
                     /gene_synonym="CLONE243; NOT4; NOT4H"
                     /inference="alignment:Splign:2.1.0"
     exon            1436..1933
                     /gene="CNOT4"
                     /gene_synonym="CLONE243; NOT4; NOT4H"
                     /inference="alignment:Splign:2.1.0"
     exon            1934..2146
                     /gene="CNOT4"
                     /gene_synonym="CLONE243; NOT4; NOT4H"
                     /inference="alignment:Splign:2.1.0"
     exon            2147..3538
                     /gene="CNOT4"
                     /gene_synonym="CLONE243; NOT4; NOT4H"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3512..3517
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CNOT4"
                     /gene_synonym="CLONE243; NOT4; NOT4H"
                     /note="hexamer: AATAAA"
     polyA_site      3538
                     /gene="CNOT4"
                     /gene_synonym="CLONE243; NOT4; NOT4H"
                     /note="major polyA site"
ORIGIN      
        1 ggaggagtct taccctaatg taagatggtg gcccgtctgg ctgtggaacc caccgaaagc
       61 tgagagtctc tctctcagcc gtaaaggtcg gactcgctga agcaatcagg cccggttctg
      121 aagagttttt tccccgacgc tcgttcggtg gtttcttgtg aggacgcggc agcccgtgga
      181 gtcggctgag ggaaaagcga ggcaaggcag gcgggtcgtc aaagtcctac attttagctt
      241 tcagtgctga acttccttct cccttaaatt attataaact tttgctgctg tttaataaac
      301 gtgaagatgt ctcgcagtcc tgatgcgaag gaagaccctg tggagtgccc tctttgcatg
      361 gagcccttgg agatagatga tatcaacttt ttcccttgca cctgtggcta ccagatttgc
      421 cgattttgtt ggcatcgaat tcgcactgat gaaaatgggc tttgtcctgc atgtagaaag
      481 ccatatccag aagacccagc agtttataaa ccactctccc aggaagagct gcaaaggata
      541 aagaatgaga aaaaacagaa acaaaatgag agaaaacaga aaatatcaga aaatcgcaaa
      601 catttggcta gtgtacgtgt cgtacaaaaa aacctcgtct ttgttgtagg tttatctcag
      661 cgcctagcag acccagaggt tttaaaacga ccagaatatt ttgggaagtt tggtaaaata
      721 cataaagttg tcatcaataa tagcacatca tatgcaggct cacagggtcc aagtgccagt
      781 gcttatgtaa cctatatccg gtcagaagac gctctcagag ccatacagtg tgtcaacaat
      841 gtggtagtag atggcagaac acttaaggca tctctaggta caacaaaata ctgcagttac
      901 ttcttaaaga atatgcagtg tccaaaacct gactgcatgt atcttcatga attgggggat
      961 gaggcggcca gcttcacaaa agaggaaatg caggcgggta aacaccaaga atatgaacag
     1021 aagctacttc aagaattata taaattaaat cccaattttc ttcagctatc tacgggttca
     1081 gttgataaaa ataagaacaa agtgacacca ctgcagaggt acgatacccc cattgacaaa
     1141 ccttcagatt ctctcagtat agggaacggt gataattccc agcagatatc taacagtgat
     1201 acgccttcac caccacctgg tttgtcaaaa tccaatccag tcatccccat cagttcatcc
     1261 aatcacagtg cacggtcccc ttttgaaggg gcagtaacag agtcacagtc gttattctca
     1321 gacaattttc gccatcccaa ccctatccca agtgggcttc ctcctttccc cagctcccca
     1381 cagacatcca gtgactggcc tacagcacca gaaccacaga gcctcttcac atcagaaaca
     1441 atcccagtat catcctctac agactggcaa gcagcttttg gctttggttc ttctaaacaa
     1501 ccagaggatg acttgggttt tgatcccttc gatgtcactc gaaaagcctt agcagacctg
     1561 attgagaagg aactgtccgt tcaagaccaa ccttcccttt cgcccacatc tcttcagaac
     1621 tcctcttcac acactacaac cgccaaaggt ccaggctctg gattcctgca tcctgctgca
     1681 gctacaaatg ccaattctct caatagtacc ttttcagtct tgccccagag gttccctcaa
     1741 tttcagcagc accgagcggt ttataattca ttcagttttc caggccaggc agcccgctat
     1801 ccttggatgg cctttccacg caatagcatc atgcacttga accacacagc aaaccccacc
     1861 tcaaatagta atttcttgga cttgaatctc ccgccacagc acaacacagg tctgggaggg
     1921 atccctgtag cagacaacag cagttctgta gagagtttaa atatgaagga atggcaggac
     1981 gggctaaggg cacttctacc caacattaac atcaactttg gtggactgcc caattcttct
     2041 tccccctcca acgccaacca cagtgcacca acgtccaaca ctgccaccac cgacagcctg
     2101 agttgggaca gccctggcag ctggacagac ccagccatca tcacaggtat tccagcgtct
     2161 tcaggaaaca gtttagactc tcttcaagat gacaatcctc cacactggct aaaatccctt
     2221 caggccctca cagagatgga cggccccagc gctgctccat cacagaccca ccacagcgcc
     2281 cccttcagca cacagatccc gctgcacaga gccagttgga atccctaccc tcctccttca
     2341 aacccttcca gcttccactc cccaccccca ggctttcaga cagccttcag accccccagc
     2401 aaaaccccca cagatttact acagagttca acactggacc gccattaggc aaagaggagc
     2461 aaccattaga aaatgcagga tttgtcccac tctgttttct ccctctcagc ccaccaccca
     2521 cagctccctt ctcatctctt atgttctgaa gaatccagta cctgatcaat tttttttctc
     2581 cctaacccca gatgcaatgc gatcacaggg tcattaccat ctctttatta attgtaaaaa
     2641 tttttgttga tccagcattt gagtgacact gttgaatgtt tctaaaaatg cctttttaag
     2701 gagagaaaaa aaaatcaaag ggcagtctca atacttagaa aattattgtt tgtctgttgc
     2761 gcataataat gacataatct gcttagcaga aaatgacgat taatctatag gaaagctcaa
     2821 gtaaatgcat tatcaactgc agaagtttga aaaccaggtt catttacgtg agattgctaa
     2881 atgcatgggg gaaagcagtg gtcctagcat ccatcttgta ttcagcttat cattattgca
     2941 gggaaaatgc ttttaattta aattaatttt taattctttt ggcaagttga tggcaaggac
     3001 ttgattgtgt cattaagcaa aagaatgtat tggaagttga tggaaagaca aattcatctg
     3061 tagtagtaac tggccgattg ctaaagagtt cataaggagg tgagaagtaa tttttttaaa
     3121 ggagaaaaat tttttggctt tagatttaaa gtaaattgaa atgttttaaa gaaaaaagta
     3181 ttcacagatt taatacctat taataatata agagctgaaa tgtaagtcat ttcttcagtc
     3241 cttctcctct gtcggaatct tttttgtttt accataaatt cacctgacga gggcactctg
     3301 agatagcact gctctggggc catctgatca ccatcgggag caaatctctg acctccttgc
     3361 ctgcagcttt tacttaaccc tgtagtttct ggacgtttgt gcagtattga aaagacagga
     3421 gaaaagaaaa cagaaacctg gttataacct gacgctaaaa ctaaaaacaa ggaaatgtac
     3481 ctctttcttc agaattaaaa ctaaaatctt aaataaaaca gaaaacttga tggtgaca
//
