LOCUS       NM_014515               2844 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens CCR4-NOT transcription complex subunit 2 (CNOT2),
            transcript variant 2, mRNA.
ACCESSION   NM_014515 NM_016398 XM_001723205 XM_001724308 XM_001726571
VERSION     NM_014515.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Jung,J.H., Lee,D., Ko,H.M. and Jang,H.J.
  TITLE     Inhibition of CNOT2 Induces Apoptosis via MID1IP1 in Colorectal
            Cancer Cells by Activating p53
  JOURNAL   Biomolecules 11 (10), 1492 (2021)
   PUBMED   34680125
  REMARK    GeneRIF: Inhibition of CNOT2 Induces Apoptosis via MID1IP1 in
            Colorectal Cancer Cells by Activating p53.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Royer-Bertrand,B., Cisarova,K., Niel Butschi,F., Foletti,G.,
            Guinchat,V., Tran,C., Superti-Furga,A. and Good,J.M.
  TITLE     CNOT2 haploinsufficiency in a 40-year-old man with intellectual
            disability, autism, and seizures
  JOURNAL   Am J Med Genet A 185 (8), 2602-2606 (2021)
   PUBMED   34018673
  REMARK    GeneRIF: CNOT2 haploinsufficiency in a 40-year-old man with
            intellectual disability, autism, and seizures.
            Review article
REFERENCE   3  (bases 1 to 2844)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 2844)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 2844)
  AUTHORS   Jayne,S., Zwartjes,C.G., van Schaik,F.M. and Timmers,H.T.
  TITLE     Involvement of the SMRT/NCoR-HDAC3 complex in transcriptional
            repression by the CNOT2 subunit of the human Ccr4-Not complex
  JOURNAL   Biochem J 398 (3), 461-467 (2006)
   PUBMED   16712523
  REMARK    GeneRIF: This study shows that SMRT/NCoR-HDAC3 complex is a
            cofactor of CNOT2-mediated repression and suggests that
            transcriptional regulation by the Ccr4-Not complex involves
            regulation of chromatin modification.
REFERENCE   6  (bases 1 to 2844)
  AUTHORS   Winkler,G.S., Mulder,K.W., Bardwell,V.J., Kalkhoven,E. and
            Timmers,H.T.
  TITLE     Human Ccr4-Not complex is a ligand-dependent repressor of nuclear
            receptor-mediated transcription
  JOURNAL   EMBO J 25 (13), 3089-3099 (2006)
   PUBMED   16778766
REFERENCE   7  (bases 1 to 2844)
  AUTHORS   Shi,J. and Nelson,M.A.
  TITLE     The cyclin-dependent kinase 11 interacts with NOT2
  JOURNAL   Biochem Biophys Res Commun 334 (4), 1310-1316 (2005)
   PUBMED   16039607
  REMARK    GeneRIF: These findings suggest that CDK11 may contribute to
            apoptosis by regulating the activity of NOT2 independent of its
            kinase activity.
REFERENCE   8  (bases 1 to 2844)
  AUTHORS   Zwartjes,C.G., Jayne,S., van den Berg,D.L. and Timmers,H.T.
  TITLE     Repression of promoter activity by CNOT2, a subunit of the
            transcription regulatory Ccr4-not complex
  JOURNAL   J Biol Chem 279 (12), 10848-10854 (2004)
   PUBMED   14707134
  REMARK    GeneRIF: major repression function of CNOT2 is localized in a
            specialized protein motif, the Not-Box
REFERENCE   9  (bases 1 to 2844)
  AUTHORS   Denis,C.L. and Chen,J.
  TITLE     The CCR4-NOT complex plays diverse roles in mRNA metabolism
  JOURNAL   Prog Nucleic Acid Res Mol Biol 73, 221-250 (2003)
   PUBMED   12882519
  REMARK    Review article
REFERENCE   10 (bases 1 to 2844)
  AUTHORS   Albert,T.K., Lemaire,M., van Berkum,N.L., Gentz,R., Collart,M.A.
            and Timmers,H.T.
  TITLE     Isolation and characterization of human orthologs of yeast CCR4-NOT
            complex subunits
  JOURNAL   Nucleic Acids Res 28 (3), 809-817 (2000)
   PUBMED   10637334
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC092881.8 and AC025569.37.
            
            On May 6, 2020 this sequence version replaced NM_014515.6.
            
            Summary: This gene encodes a subunit of the multi-component
            CCR4-NOT complex. The CCR4-NOT complex regulates mRNA synthesis and
            degradation and is also thought to be involved in mRNA splicing,
            transport and localization. The encoded protein interacts with
            histone deacetylases and functions as a repressor of polymerase II
            transcription. Alternatively spliced transcript variants have been
            observed for this gene. [provided by RefSeq, Dec 2010].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1-3 and 5 all encode the same isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038197.720316.1,
                                           SRR14038192.853501.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000229195.8/ ENSP00000229195.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-48                AC092881.8         108784-108831       c
            49-191              AC092881.8         73990-74132         c
            192-314             AC092881.8         41247-41369         c
            315-381             AC092881.8         32900-32966         c
            382-529             AC092881.8         22694-22841         c
            530-712             AC092881.8         21795-21977         c
            713-792             AC092881.8         19418-19497         c
            793-918             AC092881.8         16701-16826         c
            919-1043            AC092881.8         14751-14875         c
            1044-1164           AC092881.8         13701-13821         c
            1165-1321           AC092881.8         13442-13598         c
            1322-1383           AC092881.8         10096-10157         c
            1384-1433           AC092881.8         9957-10006          c
            1434-1534           AC092881.8         8036-8136           c
            1535-1679           AC092881.8         5940-6084           c
            1680-2844           AC025569.37        186078-187242       c
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q15"
     gene            1..2844
                     /gene="CNOT2"
                     /gene_synonym="CDC36; HSPC131; IDNADFS; NOT2; NOT2H"
                     /note="CCR4-NOT transcription complex subunit 2"
                     /db_xref="GeneID:4848"
                     /db_xref="HGNC:HGNC:7878"
                     /db_xref="MIM:604909"
     exon            1..48
                     /gene="CNOT2"
                     /gene_synonym="CDC36; HSPC131; IDNADFS; NOT2; NOT2H"
                     /inference="alignment:Splign:2.1.0"
     exon            49..191
                     /gene="CNOT2"
                     /gene_synonym="CDC36; HSPC131; IDNADFS; NOT2; NOT2H"
                     /inference="alignment:Splign:2.1.0"
     CDS             144..1766
                     /gene="CNOT2"
                     /gene_synonym="CDC36; HSPC131; IDNADFS; NOT2; NOT2H"
                     /note="isoform a is encoded by transcript variant 2;
                     negative regulator of transcription 2; CCR4-associated
                     factor 2; CC chemokine receptor 4-negative regulator of
                     transcription 2"
                     /codon_start=1
                     /product="CCR4-NOT transcription complex subunit 2 isoform
                     a"
                     /protein_id="NP_055330.1"
                     /db_xref="CCDS:CCDS31857.1"
                     /db_xref="GeneID:4848"
                     /db_xref="HGNC:HGNC:7878"
                     /db_xref="MIM:604909"
                     /translation="MVRTDGHTLSEKRNYQVTNSMFGASRKKFVEGVDSDYHDENMYY
                     SQSSMFPHRSEKDMLASPSTSGQLSQFGASLYGQQSALGLPMRGMSNNTPQLNRSLSQ
                     GTQLPSHVTPTTGVPTMSLHTPPSPSRGILPMNPRNMMNHSQVGQGIGIPSRTNSMSS
                     SGLGSPNRSSPSIICMPKQQPSRQPFTVNSMSGFGMNRNQAFGMNNSLSSNIFNGTDG
                     SENVTGLDLSDFPALADRNRREGSGNPTPLINPLAGRAPYVGMVTKPANEQSQDFSIH
                     NEDFPALPGSSYKDPTSSNDDSKSNLNTSGKTTSSTDGPKFPGDKSSTTQNNNQQKKG
                     IQVLPDGRVTNIPQGMVTDQFGMIGLLTFIRAAETDPGMVHLALGSDLTTLGLNLNSP
                     ENLYPKFASPWASSPCRPQDIDFHVPSEYLTNIHIRDKLAAIKLGRYGEDLLFYLYYM
                     NGGDVLQLLAAVELFNRDWRYHKEERVWITRAPGMEPTMKTNTYERGTYYFFDCLNWR
                     KVAKEFHLEYDKLEERPHLPSTFNYNPAQQAF"
     exon            192..314
                     /gene="CNOT2"
                     /gene_synonym="CDC36; HSPC131; IDNADFS; NOT2; NOT2H"
                     /inference="alignment:Splign:2.1.0"
     exon            315..381
                     /gene="CNOT2"
                     /gene_synonym="CDC36; HSPC131; IDNADFS; NOT2; NOT2H"
                     /inference="alignment:Splign:2.1.0"
     exon            382..529
                     /gene="CNOT2"
                     /gene_synonym="CDC36; HSPC131; IDNADFS; NOT2; NOT2H"
                     /inference="alignment:Splign:2.1.0"
     exon            530..712
                     /gene="CNOT2"
                     /gene_synonym="CDC36; HSPC131; IDNADFS; NOT2; NOT2H"
                     /inference="alignment:Splign:2.1.0"
     exon            713..792
                     /gene="CNOT2"
                     /gene_synonym="CDC36; HSPC131; IDNADFS; NOT2; NOT2H"
                     /inference="alignment:Splign:2.1.0"
     exon            793..918
                     /gene="CNOT2"
                     /gene_synonym="CDC36; HSPC131; IDNADFS; NOT2; NOT2H"
                     /inference="alignment:Splign:2.1.0"
     exon            919..1043
                     /gene="CNOT2"
                     /gene_synonym="CDC36; HSPC131; IDNADFS; NOT2; NOT2H"
                     /inference="alignment:Splign:2.1.0"
     exon            1044..1164
                     /gene="CNOT2"
                     /gene_synonym="CDC36; HSPC131; IDNADFS; NOT2; NOT2H"
                     /inference="alignment:Splign:2.1.0"
     exon            1165..1321
                     /gene="CNOT2"
                     /gene_synonym="CDC36; HSPC131; IDNADFS; NOT2; NOT2H"
                     /inference="alignment:Splign:2.1.0"
     exon            1322..1383
                     /gene="CNOT2"
                     /gene_synonym="CDC36; HSPC131; IDNADFS; NOT2; NOT2H"
                     /inference="alignment:Splign:2.1.0"
     exon            1384..1433
                     /gene="CNOT2"
                     /gene_synonym="CDC36; HSPC131; IDNADFS; NOT2; NOT2H"
                     /inference="alignment:Splign:2.1.0"
     exon            1434..1534
                     /gene="CNOT2"
                     /gene_synonym="CDC36; HSPC131; IDNADFS; NOT2; NOT2H"
                     /inference="alignment:Splign:2.1.0"
     exon            1535..1679
                     /gene="CNOT2"
                     /gene_synonym="CDC36; HSPC131; IDNADFS; NOT2; NOT2H"
                     /inference="alignment:Splign:2.1.0"
     exon            1680..2844
                     /gene="CNOT2"
                     /gene_synonym="CDC36; HSPC131; IDNADFS; NOT2; NOT2H"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2821..2826
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CNOT2"
                     /gene_synonym="CDC36; HSPC131; IDNADFS; NOT2; NOT2H"
                     /note="hexamer: AATAAA"
     polyA_site      2844
                     /gene="CNOT2"
                     /gene_synonym="CDC36; HSPC131; IDNADFS; NOT2; NOT2H"
                     /note="major polyA site"
ORIGIN      
        1 ggtggatacg tcgccatctt ggatccgcgg gacaagaaaa ttcatgcgag ggagacgtgg
       61 tgggcggtcc ttcctgtgac acgacccttg agtgacagtt ctatttgatt gcctccggta
      121 ctgtgaggaa aggacacgac tctatggtga ggactgatgg acatacatta tctgagaaaa
      181 gaaactacca ggtgacaaac agcatgtttg gtgcttcaag aaagaagttt gtagaggggg
      241 tcgacagtga ctaccatgac gaaaacatgt actacagcca gtcttctatg tttccacatc
      301 ggtcagaaaa agatatgctg gcatcaccat ctacatcagg tcagctgtct cagtttgggg
      361 caagtttata cgggcaacaa agtgcactag gccttccaat gagggggatg agcaacaata
      421 cccctcagtt aaatcgcagc ttatcacaag gcactcagtt accgagccac gtcacgccaa
      481 caacaggggt accaacaatg tcacttcaca cgcctccatc tccaagcagg ggtattttgc
      541 ctatgaatcc taggaatatg atgaaccact cccaggttgg tcagggcatt ggaattccta
      601 gcaggacaaa tagcatgagc agttcagggt taggtagccc caacagaagc tcgccaagca
      661 taatatgtat gccaaagcag cagccttctc gacagccttt tactgtgaac agtatgtctg
      721 gatttggaat gaacaggaat caggcatttg gaatgaataa ctccttatca agtaacattt
      781 ttaatggaac agacggaagt gaaaatgtga caggattgga cctttcagat ttcccagcat
      841 tagcagaccg aaacaggagg gaaggaagtg gtaacccaac tccattaata aaccccttgg
      901 ctggaagagc tccttatgtt ggaatggtaa caaaaccagc aaatgaacaa tcccaggact
      961 tctcaataca caatgaagat tttccagcat taccaggctc cagctataaa gatccaacat
     1021 caagtaatga tgacagtaaa tctaatttga atacatctgg caagacaact tcaagtacag
     1081 atggacccaa attccctgga gataaaagtt caacaacaca aaataataac cagcagaaaa
     1141 aagggatcca ggtgttacct gatggtcggg ttactaacat tcctcaaggg atggtgacgg
     1201 accaatttgg aatgattggc ctgttaacat ttatcagggc agcagagaca gacccaggaa
     1261 tggtacatct tgcattagga agtgacttaa caacattagg cctcaatctg aactctcctg
     1321 aaaatctcta ccccaaattt gcgtcaccct gggcatcttc accttgtcga cctcaagaca
     1381 tagacttcca tgttccatct gagtacttaa cgaacattca cattagggat aagctggctg
     1441 caataaaact tggccgatat ggtgaagacc ttctcttcta tctctattac atgaatggag
     1501 gagacgtatt acaactttta gctgcagtgg agctttttaa ccgtgattgg agataccaca
     1561 aagaagaacg agtatggatt accagggcac caggcatgga gccaacaatg aaaaccaata
     1621 cctatgagag gggaacatat tacttctttg actgtcttaa ctggaggaaa gtagctaagg
     1681 agttccatct ggaatatgac aaattagaag aacggcctca cctgccatcc accttcaact
     1741 acaaccctgc tcagcaagcc ttctaaaaaa aaaaaaaaaa aaaaaaaaag acttcccttt
     1801 tcttggggta tggctgtctc agcacaatac tcaacataac tgcagaactg atgtggctca
     1861 ggcaccctgg ttttaattcc ttgaggatct ggcaattggc ttacgcaaaa ggtcaccatt
     1921 tgaggtcctg ccttactaat tatgtgctgc ccaacaacta aatttgtaat ttgtttttct
     1981 ctagtttgag cagggtctga attttttcat ttatttcctt ttttgccagc agacagactt
     2041 gagtctgtaa agacaagcaa atacactgac agaagtttac catagtttct aaaatgtaaa
     2101 aaagaaaacc cccaaaagac tcaagaaaat tagaccacaa attttgcatt gttcattgta
     2161 gcactattgg taataaaata acaaatgttt gtgcattttt atgtgaagat ccttctcgta
     2221 tttcatttgg aaagatgagc aagaggtctg cttccttcat tttacttccc cttctgtttt
     2281 tgaaaggcag tttcgccaag cttaatgcaa gaatatctga ctgtttagaa gaaagatatt
     2341 gccacaatct ctggatggtt ttccagggtt gtgttattac tgagcttcat ctttccagaa
     2401 tgagcaaaac actgtccagt ctttgttacg attttgtaat aaatgtgtac atttttttta
     2461 aatttttgga catcacatga ataaaggtat gtatgtacga atgtgtatat attatatata
     2521 tgacatctat tttggaaaat gtttgccctg ctgtacctca tttttaggag gtgtgcatgg
     2581 atgcaatata tgaaaatggg acattctgga actgctggtc aggggacttt gtcgccctgt
     2641 gcactaaaag ggccagattt tcagcagcca aggacatcca tacccaagtg aatgtgatgg
     2701 gacttaaaag aagtgaactg agacaattca ctctggctgt ttgaacagca gcgtttcata
     2761 ggaagagaaa aaaagatcaa tcttgtattt tctgaccaca taaaggcttc ttctctttgt
     2821 aataaagtag aaaagctctc ctca
//
