LOCUS       NP_060093                707 aa            linear   PRI 09-FEB-2025
DEFINITION  metal transporter CNNM3 isoform 1 precursor [Homo sapiens].
ACCESSION   NP_060093
VERSION     NP_060093.3
DBSOURCE    REFSEQ: accession NM_017623.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 707)
  AUTHORS   Bai,Z., Feng,J., Franken,G.A.C., Al'Saadi,N., Cai,N., Yu,A.S.,
            Lou,L., Komiya,Y., Hoenderop,J.G.J., de Baaij,J.H.F., Yue,L. and
            Runnels,L.W.
  TITLE     CNNM proteins selectively bind to the TRPM7 channel to stimulate
            divalent cation entry into cells
  JOURNAL   PLoS Biol 19 (12), e3001496 (2021)
   PUBMED   34928937
  REMARK    GeneRIF: CNNM proteins selectively bind to the TRPM7 channel to
            stimulate divalent cation entry into cells.
            Publication Status: Online-Only
REFERENCE   2  (residues 1 to 707)
  AUTHORS   Xu,H., Chai,S.S., Lv,P. and Wang,J.J.
  TITLE     CNN3 in glioma: The prognostic factor and a potential
            immunotherapeutic target
  JOURNAL   Medicine (Baltimore) 100 (46), e27931 (2021)
   PUBMED   34797350
  REMARK    GeneRIF: CNN3 in glioma: The prognostic factor and a potential
            immunotherapeutic target.
REFERENCE   3  (residues 1 to 707)
  AUTHORS   Cai,F., Huang,Y., Wang,M., Sun,M., Zhao,Y. and Hattori,M.
  TITLE     A FRET-based screening method to detect potential inhibitors of the
            binding of CNNM3 to PRL2
  JOURNAL   Sci Rep 10 (1), 12879 (2020)
   PUBMED   32733084
  REMARK    GeneRIF: A FRET-based screening method to detect potential
            inhibitors of the binding of CNNM3 to PRL2.
            Publication Status: Online-Only
REFERENCE   4  (residues 1 to 707)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (residues 1 to 707)
  AUTHORS   Chen,Y.S., Kozlov,G., Fakih,R., Funato,Y., Miki,H. and Gehring,K.
  TITLE     The cyclic nucleotide-binding homology domain of the integral
            membrane protein CNNM mediates dimerization and is required for
            Mg2+ efflux activity
  JOURNAL   J Biol Chem 293 (52), 19998-20007 (2018)
   PUBMED   30341174
  REMARK    GeneRIF: This study determined the first crystal structures of the
            cyclic nucleotide-binding homology (CNBH) domain domains of CNNM2
            and CNNM3 at 2.6 and 1.9 A resolutions.
REFERENCE   6  (residues 1 to 707)
  AUTHORS   Gomez Garcia,I., Oyenarte,I. and Martinez-Cruz,L.A.
  TITLE     Purification, crystallization and preliminary crystallographic
            analysis of the CBS pair of the human metal transporter CNNM4
  JOURNAL   Acta Crystallogr Sect F Struct Biol Cryst Commun 67 (Pt 3), 349-353
            (2011)
   PUBMED   21393841
REFERENCE   7  (residues 1 to 707)
  AUTHORS   Kerns,S.L., Ostrer,H., Stock,R., Li,W., Moore,J., Pearlman,A.,
            Campbell,C., Shao,Y., Stone,N., Kusnetz,L. and Rosenstein,B.S.
  TITLE     Genome-wide association study to identify single nucleotide
            polymorphisms (SNPs) associated with the development of erectile
            dysfunction in African-American men after radiotherapy for prostate
            cancer
  JOURNAL   Int J Radiat Oncol Biol Phys 78 (5), 1292-1300 (2010)
   PUBMED   20932654
REFERENCE   8  (residues 1 to 707)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   9  (residues 1 to 707)
  AUTHORS   Venkatesan,K., Rual,J.F., Vazquez,A., Stelzl,U., Lemmens,I.,
            Hirozane-Kishikawa,T., Hao,T., Zenkner,M., Xin,X., Goh,K.I.,
            Yildirim,M.A., Simonis,N., Heinzmann,K., Gebreab,F., Sahalie,J.M.,
            Cevik,S., Simon,C., de Smet,A.S., Dann,E., Smolyar,A.,
            Vinayagam,A., Yu,H., Szeto,D., Borick,H., Dricot,A., Klitgord,N.,
            Murray,R.R., Lin,C., Lalowski,M., Timm,J., Rau,K., Boone,C.,
            Braun,P., Cusick,M.E., Roth,F.P., Hill,D.E., Tavernier,J.,
            Wanker,E.E., Barabasi,A.L. and Vidal,M.
  TITLE     An empirical framework for binary interactome mapping
  JOURNAL   Nat Methods 6 (1), 83-90 (2009)
   PUBMED   19060904
REFERENCE   10 (residues 1 to 707)
  AUTHORS   Wang,C.Y., Shi,J.D., Yang,P., Kumar,P.G., Li,Q.Z., Run,Q.G.,
            Su,Y.C., Scott,H.S., Kao,K.J. and She,J.X.
  TITLE     Molecular cloning and characterization of a novel gene family of
            four ancient conserved domain proteins (ACDP)
  JOURNAL   Gene 306, 37-44 (2003)
   PUBMED   12657465
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK126847.1 and AC092636.3.
            
            On Dec 18, 2003 this sequence version replaced NP_060093.2.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC037272.1, AK126847.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000305510.4/ ENSP00000305449.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q11.2"
     Protein         1..707
                     /product="metal transporter CNNM3 isoform 1 precursor"
                     /note="ancient conserved domain protein 3; metal
                     transporter CNNM3; cyclin-M3; ancient conserved
                     domain-containing protein 3"
                     /calculated_mol_wt=73791
     sig_peptide     1..25
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=2347
     Site            11..27
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8NE01.1)"
     Region          <13..299
                     /region_name="COG3903"
                     /note="Predicted ATPase [General function prediction
                     only]"
                     /db_xref="CDD:443109"
     Site            73
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8NE01.1)"
     Site            193..213
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8NE01.1)"
     Site            221..241
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8NE01.1)"
     Site            261..281
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8NE01.1)"
     Region          313..442
                     /region_name="CBS_pair_CorC_HlyC_assoc"
                     /note="Two tandem repeats of the cystathionine
                     beta-synthase (CBS pair) domains the majority of which are
                     associated with the CorC_HlyC domain; cd04590"
                     /db_xref="CDD:341366"
     Site            order(321,323..325,348..350,413,434,436,438..439,442)
                     /site_type="other"
                     /note="ligand binding site II [chemical binding]"
                     /db_xref="CDD:341366"
     Region          321..387
                     /region_name="CBS repeat"
                     /note="CBS repeat [structural motif]"
                     /db_xref="CDD:341366"
     Site            order(348,362,366..367,370,391,413..415,438)
                     /site_type="other"
                     /note="putative ligand binding site I [chemical binding]"
                     /db_xref="CDD:341366"
     Region          391..442
                     /region_name="CBS repeat"
                     /note="CBS repeat [structural motif]"
                     /db_xref="CDD:341366"
     Site            661
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:20068231,
                     ECO:0007744|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (Q8NE01.1)"
     Region          678..707
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8NE01.1)"
     Site            700
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (Q8NE01.1)"
     CDS             1..707
                     /gene="CNNM3"
                     /gene_synonym="ACDP3; SLC70A3"
                     /coded_by="NM_017623.5:11..2134"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1"
                     /db_xref="CCDS:CCDS2025.1"
                     /db_xref="GeneID:26505"
                     /db_xref="HGNC:HGNC:104"
                     /db_xref="MIM:607804"
ORIGIN      
        1 maaavaaagr lgwlfaalcl gnaageaapg prvlgfclee dgaagagwvr ggaardtpda
       61 tfllrlfgpg fansswswva pegagcreea aspagewral lrlrlraeav rphsallavr
      121 vepgggaaee aappwalglg aagllalaal arglqlsala lapaevqvlr esgseaeraa
      181 arrleparrw agcalgalll laslaqaala vllyraagqr avpavlgsag lvflvgevvp
      241 aavsgrwtla lapralglsr lavlltlpva lpvgqllela arpgrlrerv lelargggdp
      301 ysdlskgvlr crtvedvltp ledcfmldas tvldfgvlas imqsghtrip vyeeersniv
      361 dmlylkdlaf vdpedctpls titrfynhpl hfvfndtkld avleefkrgk shlaivqkvn
      421 negegdpfye vlglvtledv ieeiirseil desedyrdtv vkrkpaslma plkrkeefsl
      481 fkvsddeykv tispqlllat qrflsrevdv fsplrisekv llhllkhpsv nqevrfdesn
      541 rlathhylyq rsqpvdyfil ilqgrvevei gkeglkfeng aftyygvsal tvpssvhqsp
      601 vsslqpirhd lqpdpgdgth ssaycpdytv ralsdlqlik vtrlqylnal latraqnlpq
      661 spentdlqvi pgsqtrllge ktttaagssh srpgvpvegs pgrnpgv
//
