LOCUS       NM_004368               2149 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens calponin 2 (CNN2), transcript variant 1, mRNA.
ACCESSION   NM_004368
VERSION     NM_004368.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Gui,Y., Palanza,Z., Gupta,P., Li,H., Pan,Y., Wang,Y., Hargis,G.,
            Kreutzer,D.L., Wang,Y., Bastacky,S.I., Liu,Y., Liu,S. and Zhou,D.
  TITLE     Calponin 2 regulates ketogenesis to mitigate acute kidney injury
  JOURNAL   JCI Insight 8 (21), e170521 (2023)
   PUBMED   37751293
  REMARK    GeneRIF: Calponin 2 regulates ketogenesis to mitigate acute kidney
            injury.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Zhang,R., Li,S., Wang,Y., Cai,W., Liu,Q. and Zhang,J.
  TITLE     Serum calponin 2 is a novel biomarker of tubal ectopic pregnancy
  JOURNAL   Fertil Steril 116 (4), 1020-1027 (2021)
   PUBMED   34217487
  REMARK    GeneRIF: Serum calponin 2 is a novel biomarker of tubal ectopic
            pregnancy.
REFERENCE   3  (bases 1 to 2149)
  AUTHORS   Qian,A., Hsieh,T.B., Hossain,M.M., Lin,J.J. and Jin,J.P.
  TITLE     A rapid degradation of calponin 2 is required for cytokinesis
  JOURNAL   Am J Physiol Cell Physiol 321 (2), C355-C368 (2021)
   PUBMED   34133238
  REMARK    GeneRIF: A rapid degradation of calponin 2 is required for
            cytokinesis.
REFERENCE   4  (bases 1 to 2149)
  AUTHORS   Kang,X., Wang,F., Lan,X., Li,X., Zheng,S., Lv,Z., Zhuang,Y.,
            Zhao,Y. and Zhou,S.
  TITLE     Lentivirus-mediated shRNA Targeting CNN2 Inhibits Hepatocarcinoma
            in Vitro and in Vivo
  JOURNAL   Int J Med Sci 15 (1), 69-76 (2018)
   PUBMED   29333089
  REMARK    GeneRIF: Study in human hepatocellular carcinoma cell line and
            xenograft mouse model shows that CNN2 plays an important role in
            tumor growth and metastasis, possibly through MEK1/2-ERK1/2
            signaling pathway.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2149)
  AUTHORS   Hu,J., Xie,W., Shang,L., Yang,X., Li,Q., Xu,M., Dou,J., Zhou,Y.,
            Niu,W. and Wu,Y.
  TITLE     Knockdown of calponin 2 suppressed cell growth in gastric cancer
            cells
  JOURNAL   Tumour Biol 39 (7), 1010428317706455 (2017)
   PUBMED   28714360
  REMARK    GeneRIF: our results demonstrated that knockdown of endogenous CNN2
            in AGS cells could significantly activate cell apoptosis pathway
            and therefore suppress cell growth in vitro. The deletion of CNN2
            might be a potential therapeutic approach to inhibit aggressive
            growth of gastric cancer.
REFERENCE   6  (bases 1 to 2149)
  AUTHORS   Gevaert,K., Goethals,M., Martens,L., Van Damme,J., Staes,A.,
            Thomas,G.R. and Vandekerckhove,J.
  TITLE     Exploring proteomes and analyzing protein processing by mass
            spectrometric identification of sorted N-terminal peptides
  JOURNAL   Nat Biotechnol 21 (5), 566-569 (2003)
   PUBMED   12665801
REFERENCE   7  (bases 1 to 2149)
  AUTHORS   Hossain,M.M., Hwang,D.Y., Huang,Q.Q., Sasaki,Y. and Jin,J.P.
  TITLE     Developmentally regulated expression of calponin isoforms and the
            effect of h2-calponin on cell proliferation
  JOURNAL   Am J Physiol Cell Physiol 284 (1), C156-C167 (2003)
   PUBMED   12388067
REFERENCE   8  (bases 1 to 2149)
  AUTHORS   Kuromitsu,J., Yamashita,H., Kataoka,H., Takahara,T., Muramatsu,M.,
            Sekine,T., Okamoto,N., Furuichi,Y. and Hayashizaki,Y.
  TITLE     A unique downregulation of h2-calponin gene expression in Down
            syndrome: a possible attenuation mechanism for fetal survival by
            methylation at the CpG island in the trisomic chromosome 21
  JOURNAL   Mol Cell Biol 17 (2), 707-712 (1997)
   PUBMED   9001224
REFERENCE   9  (bases 1 to 2149)
  AUTHORS   Fukui,Y., Masuda,H., Takagi,M., Takahashi,K. and Kiyokane,K.
  TITLE     The presence of h2-calponin in human keratinocyte
  JOURNAL   J Dermatol Sci 14 (1), 29-36 (1997)
   PUBMED   9049805
REFERENCE   10 (bases 1 to 2149)
  AUTHORS   Masuda,H., Tanaka,K., Takagi,M., Ohgami,K., Sakamaki,T., Shibata,N.
            and Takahashi,K.
  TITLE     Molecular cloning and characterization of human non-smooth muscle
            calponin
  JOURNAL   J Biochem 120 (2), 415-424 (1996)
   PUBMED   8889829
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY174321.1, BC141818.1,
            AC011558.6 and AA283126.1.
            
            On Nov 22, 2018 this sequence version replaced NM_004368.3.
            
            Summary: The protein encoded by this gene, which can bind actin,
            calmodulin, troponin C, and tropomyosin, may function in the
            structural organization of actin filaments. The encoded protein
            could play a role in smooth muscle contraction and cell adhesion.
            Several pseudogenes of this gene have been identified, and are
            present on chromosomes 1, 2, 3, 6, 9, 11, 13, 15, 16, 21 and 22.
            Alternative splicing results in multiple transcript variants
            encoding different isoforms. [provided by RefSeq, Jan 2015].
            
            Transcript Variant: This variant (1) uses an alternate in-frame
            splice site in the central coding region, compared to variant 4.
            The encoded isoform (a) is shorter than isoform d.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163657.164181.1,
                                           SRR3476690.369674.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000263097.9/ ENSP00000263097.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-24                HY174321.1         334-357
            25-437              BC141818.1         1-413
            438-438             AC011558.6         5756-5756
            439-1066            BC141818.1         415-1042
            1067-1067           AC011558.6         11049-11049
            1068-2148           BC141818.1         1043-2123
            2149-2149           AA283126.1         27-27               c
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.3"
     gene            1..2149
                     /gene="CNN2"
                     /note="calponin 2"
                     /db_xref="GeneID:1265"
                     /db_xref="HGNC:HGNC:2156"
                     /db_xref="MIM:602373"
     exon            1..117
                     /gene="CNN2"
                     /inference="alignment:Splign:2.1.0"
     CDS             55..984
                     /gene="CNN2"
                     /note="isoform a is encoded by transcript variant 1;
                     calponin H2, smooth muscle; neutral calponin"
                     /codon_start=1
                     /product="calponin-2 isoform a"
                     /protein_id="NP_004359.1"
                     /db_xref="CCDS:CCDS12053.1"
                     /db_xref="GeneID:1265"
                     /db_xref="HGNC:HGNC:2156"
                     /db_xref="MIM:602373"
                     /translation="MSSTQFNKGPSYGLSAEVKNRLLSKYDPQKEAELRTWIEGLTGL
                     SIGPDFQKGLKDGTILCTLMNKLQPGSVPKINRSMQNWHQLENLSNFIKAMVSYGMNP
                     VDLFEANDLFESGNMTQVQVSLLALAGKAKTKGLQSGVDIGVKYSEKQERNFDDATMK
                     AGQCVIGLQMGTNKCASQSGMTAYGTRRHLYDPKNHILPPMDHSTISLQMGTNKCASQ
                     VGMTAPGTRRHIYDTKLGTDKCDNSSMSLQMGYTQGANQSGQVFGLGRQIYDPKYCPQ
                     GTVADGAPSGTGDCPDPGEVPEYPPYYQEEAGY"
     exon            118..239
                     /gene="CNN2"
                     /inference="alignment:Splign:2.1.0"
     exon            240..306
                     /gene="CNN2"
                     /inference="alignment:Splign:2.1.0"
     exon            307..444
                     /gene="CNN2"
                     /inference="alignment:Splign:2.1.0"
     exon            445..561
                     /gene="CNN2"
                     /inference="alignment:Splign:2.1.0"
     exon            562..708
                     /gene="CNN2"
                     /inference="alignment:Splign:2.1.0"
     exon            709..2149
                     /gene="CNN2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2125..2130
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CNN2"
                     /note="hexamer: AATAAA"
     polyA_site      2149
                     /gene="CNN2"
                     /note="major polyA site"
ORIGIN      
        1 ggtcccgtcc cgtcccgtcc tgtgcggccc cgtcccgccg cccgcccgcc agccatgagc
       61 tccacgcagt tcaacaaggg cccctcgtac gggctgtcgg ccgaggtcaa gaaccggctc
      121 ctgtccaaat atgaccccca gaaggaggca gagctccgca cctggatcga gggactcacc
      181 ggcctctcca tcggccccga cttccagaag ggcctgaagg atggaactat cttatgcaca
      241 ctcatgaaca agctacagcc gggctccgtc cccaagatca accgctccat gcagaactgg
      301 caccagctag aaaacctgtc caacttcatc aaggccatgg tcagctacgg catgaaccct
      361 gtggacctgt tcgaggccaa cgacctgttt gagagtggga acatgacgca ggtgcaggtg
      421 tctcttctcg ccctggcggg gaaggccaag actaaggggc tgcagagcgg ggtggacatt
      481 ggcgtcaagt actcggagaa gcaggagcgg aatttcgacg atgccaccat gaaggctggc
      541 cagtgcgtca tcgggctgca gatgggcacc aacaaatgcg ccagccagtc gggcatgact
      601 gcctacggca cgagaaggca tctctatgac cccaagaacc atatcctgcc ccccatggac
      661 cactcgacca tcagcctcca gatgggcacg aacaagtgtg ccagccaggt gggcatgacg
      721 gctcccggga cccggcggca catctatgat accaagctgg gaaccgacaa gtgtgacaac
      781 tcctccatgt ccctgcagat gggctacacg cagggcgcca accagagcgg ccaggtcttc
      841 ggcctgggcc ggcagatata tgaccccaag tactgcccgc aaggcacagt ggccgatggg
      901 gctccctcgg gcaccggcga ctgcccggac ccgggggagg tccctgaata tcccccttac
      961 taccaggagg aggccggcta ctgaggctcc cagcacgctc tctccccaca tcgtctgccc
     1021 atctgggttt ttgggttttt ctgtgttttc atcttttttt tttttttctt aacccgttca
     1081 gtgctgccag tcaaccaagg gtctgtgagt gtcagcgtgg gatcaggcag cagagctttt
     1141 ttcccctttg ccttgatcct tcgcaaggct gagccactgg gctgtggggg aaggggtcaa
     1201 ggccatatcc caatacgtgt agggcgaggg tccctgctgg cacattcagg ctgtgctggg
     1261 aagaagagac ctgggcttgg aaggaaccgg tccccgacgg tttctgcttg cctcgcctct
     1321 tccccctttt gtcagctgag cagtttgtgg tttctatgcc cgcaagtttc aggaagtatt
     1381 cacaaaagaa aaatacattt tttcccccag gggtggggca aggacagtgg agagagtgct
     1441 aggaaatgag tcccctggga aaggggaccg ggccgtgatg ttaaatatct ccggctccca
     1501 agtgactgga tttgcctagg accttcagac caacagactt cagaccctca gacctgcccc
     1561 ggggccaggt ggagaaagtg agggccgtac aaggaagtga aattctgagt tgttggggct
     1621 aagcctgacc ccctctccat gctccccgcc ccaacccact ctggcctcag tagatttttt
     1681 tttcagttgt ggttgttgcc caggctggag tgcagtggcg ccatcttggc tcactgcacc
     1741 tccaccttcc gggctcaagc gattctccag cctcagcctc ctgagtagct aggactgcag
     1801 gtgctccacc acgcccggct aatttttgta tttttagtag agatggggtt tccccatgtt
     1861 ggccaggctg gtctcgaact cctggcctca ggtgtgatcc gcccgcctcc gcctccccaa
     1921 gcgctgagat tacaggtgtg agccaccgtg cccagcccct cagtaggttt taaggagccc
     1981 ccagccctcc tcccttctgg gcccgaccag cttatactgc tccatcttcc ccggccacat
     2041 gccccgccaa gtactgcaca gggacccccc acccagggga cctgctccgt gagataatgt
     2101 gaaatacgac tgtggaccaa acgcaataaa acctctgttt gtacgaaga
//
