LOCUS       NM_006314               2535 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens connector enhancer of kinase suppressor of Ras 1
            (CNKSR1), transcript variant 1, mRNA.
ACCESSION   NM_006314
VERSION     NM_006314.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Nishiyama,K., Maekawa,M., Nakagita,T., Nakayama,J., Kiyoi,T.,
            Chosei,M., Murakami,A., Kamei,Y., Takeda,H., Takada,Y. and
            Higashiyama,S.
  TITLE     CNKSR1 serves as a scaffold to activate an EGFR phosphatase via
            exclusive interaction with RhoB-GTP
  JOURNAL   Life Sci Alliance 4 (9), e202101095 (2021)
   PUBMED   34187934
  REMARK    GeneRIF: CNKSR1 serves as a scaffold to activate an EGFR
            phosphatase via exclusive interaction with RhoB-GTP.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 2535)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 2535)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2535)
  AUTHORS   Kazeminasab,S., Taskiran,I.I., Fattahi,Z., Bazazzadegan,N.,
            Hosseini,M., Rahimi,M., Oladnabi,M., Haddadi,M., Celik,A.,
            Ropers,H.H., Najmabadi,H. and Kahrizi,K.
  TITLE     CNKSR1 gene defect can cause syndromic autosomal recessive
            intellectual disability
  JOURNAL   Am J Med Genet B Neuropsychiatr Genet 177 (8), 691-699 (2018)
   PUBMED   30450701
  REMARK    GeneRIF: RNAi-mediated knockdown of cnk, the CNKSR1 orthologue in
            Drosophila melanogaster brain, led to defects in eye and mushroom
            body (MB) structures. Findings support the possible role of CNKSR1
            in brain development which can lead to cognitive impairment.
REFERENCE   6  (bases 1 to 2535)
  AUTHORS   Lopez-Ilasaca,M.A., Bernabe-Ortiz,J.C., Na,S.Y., Dzau,V.J. and
            Xavier,R.J.
  TITLE     Bioluminescence resonance energy transfer identify scaffold protein
            CNK1 interactions in intact cells
  JOURNAL   FEBS Lett 579 (3), 648-654 (2005)
   PUBMED   15670823
REFERENCE   7  (bases 1 to 2535)
  AUTHORS   Rabizadeh,S., Xavier,R.J., Ishiguro,K., Bernabeortiz,J.,
            Lopez-Ilasaca,M., Khokhlatchev,A., Mollahan,P., Pfeifer,G.P.,
            Avruch,J. and Seed,B.
  TITLE     The scaffold protein CNK1 interacts with the tumor suppressor
            RASSF1A and augments RASSF1A-induced cell death
  JOURNAL   J Biol Chem 279 (28), 29247-29254 (2004)
   PUBMED   15075335
  REMARK    GeneRIF: Data show that the connector enhancer of KSR 1 scaffold
            protein, through its binding of a RASSF1A/MST1 complex,
            participates in the proapoptotic signaling initiated by active Ras.
REFERENCE   8  (bases 1 to 2535)
  AUTHORS   Jaffe,A.B., Aspenstrom,P. and Hall,A.
  TITLE     Human CNK1 acts as a scaffold protein, linking Rho and Ras signal
            transduction pathways
  JOURNAL   Mol Cell Biol 24 (4), 1736-1746 (2004)
   PUBMED   14749388
  REMARK    GeneRIF: hCNK1 associates with Rhophilin and RalGDS, Rho and Ras
            effector molecules, respectively, suggesting that it acts as a
            scaffold protein to mediate cross talk between the two pathways
REFERENCE   9  (bases 1 to 2535)
  AUTHORS   Therrien,M., Wong,A.M., Kwan,E. and Rubin,G.M.
  TITLE     Functional analysis of CNK in RAS signaling
  JOURNAL   Proc Natl Acad Sci U S A 96 (23), 13259-13263 (1999)
   PUBMED   10557308
REFERENCE   10 (bases 1 to 2535)
  AUTHORS   Therrien,M., Wong,A.M. and Rubin,G.M.
  TITLE     CNK, a RAF-binding multidomain protein required for RAS signaling
  JOURNAL   Cell 95 (3), 343-353 (1998)
   PUBMED   9814705
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA445069.1, AF100153.1 and
            BC011604.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_006314.2.
            
            Summary: This gene encodes a protein containing several motifs
            involved in protein-protein interaction, including PDZ, PH
            (Pleckstrin homology), and SAM (sterile alpha motif) domains. The
            encoded protein acts as a scaffold component for receptor tyrosine
            kinase signaling and may mediate crosstalk between different
            signaling pathways. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Jul 2014].
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF100153.1, AB209335.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000361530.11/ ENSP00000354609.6
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-21                DA445069.1         2-22
            22-2115             AF100153.1         1-2094
            2116-2535           BC011604.2         2119-2538
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.11"
     gene            1..2535
                     /gene="CNKSR1"
                     /gene_synonym="CNK; CNK1"
                     /note="connector enhancer of kinase suppressor of Ras 1"
                     /db_xref="GeneID:10256"
                     /db_xref="HGNC:HGNC:19700"
                     /db_xref="MIM:603272"
     exon            1..109
                     /gene="CNKSR1"
                     /gene_synonym="CNK; CNK1"
                     /inference="alignment:Splign:2.1.0"
     CDS             58..2199
                     /gene="CNKSR1"
                     /gene_synonym="CNK; CNK1"
                     /note="isoform 1 is encoded by transcript variant 1; CNK
                     homolog protein 1; connector enhancer of KSR 1; connector
                     enhancer of KSR-like"
                     /codon_start=1
                     /product="connector enhancer of kinase suppressor of ras 1
                     isoform 1"
                     /protein_id="NP_006305.2"
                     /db_xref="CCDS:CCDS276.1"
                     /db_xref="GeneID:10256"
                     /db_xref="HGNC:HGNC:19700"
                     /db_xref="MIM:603272"
                     /translation="MEPVETWTPGKVATWLRGLDDSLQDYPFEDWQLPGKNLLQLCPQ
                     SLEALAVRSLGHQELILGGVEQLQALSSRLQTENLQSLTEGLLGATHDFQSIVQGCLG
                     DCAKTPIDVLCAAVELLHEADALLFWLSRYLFSHLNDFSACQEIRDLLEELSQVLHED
                     GPAAEKEGTVLRICSHVAGICHNILVCCPKELLEQKAVLEQVQLDSPLGLEIHTTSNC
                     QHFVSQVDTQVPTDSRLQIQPGDEVVQINEQVVVGWPRKNMVRELLREPAGLSLVLKK
                     IPIPETPPQTPPQVLDSPHQRSPSLSLAPLSPRAPSEDVFAFDLSSNPSPGPSPAWTD
                     SASLGPEPLPIPPEPPAILPAGVAGTPGLPESPDKSPVGRKKSKGLATRLSRRRVSCR
                     ELGRPDCDGWLLLRKAPGGFMGPRWRRRWFVLKGHTLYWYRQPQDEKAEGLINVSNYS
                     LESGHDQKKKYVFQLTHDVYKPFIFAADTLTDLSMWVRHLITCISKYQSPGRAPPPRE
                     EDCYSETEAEDPDDEAGSHSASPSPAQAGSPLHGDTSPAATPTQRSPRTSFGSLTDSS
                     EEALEGMVRGLRQGGVSLLGQPQPLTQEQWRSSFMRRNRDPQLNERVHRVRALQSTLK
                     AKLQELQVLEEVLGDPELTGEKFRQWKEQNRELYSEGLGAWGVAQAEGSSHILTSDST
                     EQSPHSLPSDPEEHSHLCPLTSESSLRPPDL"
     exon            110..267
                     /gene="CNKSR1"
                     /gene_synonym="CNK; CNK1"
                     /inference="alignment:Splign:2.1.0"
     exon            268..449
                     /gene="CNKSR1"
                     /gene_synonym="CNK; CNK1"
                     /inference="alignment:Splign:2.1.0"
     exon            450..534
                     /gene="CNKSR1"
                     /gene_synonym="CNK; CNK1"
                     /inference="alignment:Splign:2.1.0"
     exon            535..576
                     /gene="CNKSR1"
                     /gene_synonym="CNK; CNK1"
                     /inference="alignment:Splign:2.1.0"
     exon            577..681
                     /gene="CNKSR1"
                     /gene_synonym="CNK; CNK1"
                     /inference="alignment:Splign:2.1.0"
     exon            682..741
                     /gene="CNKSR1"
                     /gene_synonym="CNK; CNK1"
                     /inference="alignment:Splign:2.1.0"
     exon            742..810
                     /gene="CNKSR1"
                     /gene_synonym="CNK; CNK1"
                     /inference="alignment:Splign:2.1.0"
     exon            811..912
                     /gene="CNKSR1"
                     /gene_synonym="CNK; CNK1"
                     /inference="alignment:Splign:2.1.0"
     exon            913..983
                     /gene="CNKSR1"
                     /gene_synonym="CNK; CNK1"
                     /inference="alignment:Splign:2.1.0"
     exon            984..1057
                     /gene="CNKSR1"
                     /gene_synonym="CNK; CNK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1058..1164
                     /gene="CNKSR1"
                     /gene_synonym="CNK; CNK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1165..1192
                     /gene="CNKSR1"
                     /gene_synonym="CNK; CNK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1193..1365
                     /gene="CNKSR1"
                     /gene_synonym="CNK; CNK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1366..1439
                     /gene="CNKSR1"
                     /gene_synonym="CNK; CNK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1440..1511
                     /gene="CNKSR1"
                     /gene_synonym="CNK; CNK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1512..1585
                     /gene="CNKSR1"
                     /gene_synonym="CNK; CNK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1586..1647
                     /gene="CNKSR1"
                     /gene_synonym="CNK; CNK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1648..1747
                     /gene="CNKSR1"
                     /gene_synonym="CNK; CNK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1748..1929
                     /gene="CNKSR1"
                     /gene_synonym="CNK; CNK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1930..2535
                     /gene="CNKSR1"
                     /gene_synonym="CNK; CNK1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2513..2518
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CNKSR1"
                     /gene_synonym="CNK; CNK1"
                     /note="hexamer: AATAAA"
     polyA_site      2535
                     /gene="CNKSR1"
                     /gene_synonym="CNK; CNK1"
                     /note="major polyA site"
ORIGIN      
        1 aaattccggc gacagcaggg caaaacagga gctgattcga gctggcagag ctgggccatg
       61 gaaccggtag agacctggac ccccggaaag gtggcaactt ggctgagagg tcttgacgac
      121 tccctgcagg actatccctt tgaggactgg cagctgcctg gcaagaacct gctccagctc
      181 tgcccccaaa gcctcgaggc tctggctgtg cggtctctgg gacaccagga gctcatcctg
      241 ggcggggtgg aacagctcca ggccctgagc tccaggctac agacagagaa cctgcaaagc
      301 ctgacagagg gacttctggg ggcaacccat gacttccaga gcatagtcca aggctgcctg
      361 ggggactgtg ccaagacccc tattgatgtc ctctgtgcag ctgtggagct gttgcatgaa
      421 gctgacgccc tcctcttctg gctcagcagg tacctcttct cccacttaaa tgatttctca
      481 gcatgccagg agatccgaga cttgttggag gagctgagcc aggtcttgca tgaggatggt
      541 ccagcggctg agaaggaggg cacagtcctg aggatctgca gccacgtggc tgggatctgc
      601 cacaacatcc tggtctgctg ccccaaggag ctgctggaac agaaggccgt gctcgagcag
      661 gtgcagctgg acagtccatt gggcctagaa attcacacca ccagcaattg ccagcacttt
      721 gtgtcccaag tggacaccca ggttcccact gactcccgac tgcagatcca gcctggagac
      781 gaggttgtcc agatcaacga gcaggtggtg gtgggatggc cccgtaagaa catggtgagg
      841 gaactgctgc gggagccagc cggactcagc ttagtgctga agaagatccc gataccggag
      901 acccccccac agacgccccc tcaggtcctg gactccccgc accagaggag cccatcactg
      961 tctctggccc cactgtctcc cagggcccca tctgaagacg tctttgcctt tgacctgtct
     1021 tcaaacccca gtcccggacc cagccctgcc tggacagact ctgcctccct tggccctgag
     1081 cccctgccca tccccccgga acccccagcc atactcccag caggggtagc agggactcca
     1141 gggctccctg aatcccctga caagagtcct gttggtcgga agaaatcaaa aggcctggcg
     1201 acccggctga gccgccggcg ggtgtcatgc cgtgagctgg gccggccgga ctgtgacggc
     1261 tggctcctgt tgcgaaaggc accgggcggc ttcatgggcc cgcgctggcg ccgccgctgg
     1321 tttgtgctca agggacacac gctctactgg taccgccagc cccaggatga gaaggctgag
     1381 ggcctcatca atgtctccaa ctatagtctg gaaagtggac atgatcagaa gaagaaatat
     1441 gtgtttcagc tcacccatga tgtgtacaaa cccttcatct tcgctgctga taccctgaca
     1501 gatctgagca tgtgggtgcg tcatctcatt acctgcatct ccaagtacca gtctccaggc
     1561 cgggcccccc caccccgaga ggaagactgc tacagtgaga ccgaagcaga ggacccggac
     1621 gatgaggctg ggtcccactc agcctcgccc agccctgctc aagctgggag tcccctccat
     1681 ggagacacat cacctgcagc cacccccaca cagcgcagcc cacggacctc ctttggctct
     1741 ctgacagaca gcagtgaaga ggcactggaa ggaatggtac gggggctgag gcagggtggc
     1801 gtgtccctcc taggccagcc acagcccctg acccaggaac agtggcggag ctctttcatg
     1861 cggcgcaacc gagaccctca gctcaatgag cgagtgcacc gtgtgcgggc gctacagagc
     1921 acactcaagg caaagctgca ggagctgcag gtcctagaag aagtgctggg tgaccctgag
     1981 ctgacaggag agaagttccg ccagtggaag gagcagaacc gggagctgta ctcagagggc
     2041 ctgggggcct ggggagtggc acaggctgaa ggcagctccc acatcttgac ctctgactcc
     2101 acagaacagt ccccccactc cctgccctct gaccctgaag agcactccca tctctgcccc
     2161 ctgacctcag agagcagcct ccgacctcct gacctctgac cctggccagc actctagctc
     2221 ctgacctttg acccgagggc cacctcaacc ccagcttctg acgtgtccag gacagagcat
     2281 ccctggattc tgttcagggt gggaagtagt actgctagtc atggtctcac cccgagctga
     2341 cccctctgcc tgggctttgt gccaccctct cccttgccaa agaagaaact ctccccccaa
     2401 atcctccaac ctctggggcc acagccctgc ccctccagtt ccttggcagt tctcccccaa
     2461 accaggtctg tacaggtgtt ctttatttta catgagggct actttccaac caaataaagt
     2521 caatttttct aagaa
//
