LOCUS       NM_018348               4875 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens cap methyltransferase 2 (CMTR2), transcript variant 1,
            mRNA.
ACCESSION   NM_018348
VERSION     NM_018348.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Smietanski,M., Werner,M., Purta,E., Kaminska,K.H., Stepinski,J.,
            Darzynkiewicz,E., Nowotny,M. and Bujnicki,J.M.
  TITLE     Structural analysis of human 2'-O-ribose methyltransferases
            involved in mRNA cap structure formation
  JOURNAL   Nat Commun 5, 3004 (2014)
   PUBMED   24402442
  REMARK    GeneRIF: Crystal structures of the active CMTr2 catalytic domain in
            complex with a methyl group donor and a capped oligoribonucleotide
            are examined, thereby revealing the mechanism of specific
            recognition of capped RNA.
REFERENCE   2  (bases 1 to 4875)
  AUTHORS   Werner,M., Purta,E., Kaminska,K.H., Cymerman,I.A., Campbell,D.A.,
            Mittra,B., Zamudio,J.R., Sturm,N.R., Jaworski,J. and Bujnicki,J.M.
  TITLE     2'-O-ribose methylation of cap2 in human: function and evolution in
            a horizontally mobile family
  JOURNAL   Nucleic Acids Res 39 (11), 4756-4768 (2011)
   PUBMED   21310715
  REMARK    GeneRIF: A report on the validation of a human enzyme that
            methylates the ribose of the second transcribed nucleotide encoded
            by FTSJD1.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC035005.2, DB227997.1, BC034468.1, AA968997.1 and AC106736.4.
            
            On Jun 2, 2019 this sequence version replaced NM_018348.5.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.116911.1,
                                           SRR1803616.251491.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA2162568
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000434935.7/ ENSP00000411148.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-51                BC035005.2         208-258
            52-214              DB227997.1         51-213
            215-3896            BC034468.1         1-3682
            3897-3967           AA968997.1         14-84               c
            3968-4875           AC106736.4         117668-118575
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q22.2"
     gene            1..4875
                     /gene="CMTR2"
                     /gene_synonym="AFT; FTSJD1; HMTr2; MTr2"
                     /note="cap methyltransferase 2"
                     /db_xref="GeneID:55783"
                     /db_xref="HGNC:HGNC:25635"
                     /db_xref="MIM:616190"
     exon            1..267
                     /gene="CMTR2"
                     /gene_synonym="AFT; FTSJD1; HMTr2; MTr2"
                     /inference="alignment:Splign:2.1.0"
     exon            268..327
                     /gene="CMTR2"
                     /gene_synonym="AFT; FTSJD1; HMTr2; MTr2"
                     /inference="alignment:Splign:2.1.0"
     exon            328..4875
                     /gene="CMTR2"
                     /gene_synonym="AFT; FTSJD1; HMTr2; MTr2"
                     /inference="alignment:Splign:2.1.0"
     CDS             347..2659
                     /gene="CMTR2"
                     /gene_synonym="AFT; FTSJD1; HMTr2; MTr2"
                     /EC_number="2.1.1.296"
                     /note="adrift homolog; ftsJ methyltransferase
                     domain-containing protein 1; FtsJ methyltransferase domain
                     containing 1; cap2 2'O-ribose methyltransferase 2; protein
                     adrift homolog"
                     /codon_start=1
                     /product="cap-specific mRNA
                     (nucleoside-2'-O-)-methyltransferase 2"
                     /protein_id="NP_060818.4"
                     /db_xref="CCDS:CCDS10898.1"
                     /db_xref="GeneID:55783"
                     /db_xref="HGNC:HGNC:25635"
                     /db_xref="MIM:616190"
                     /translation="MSKCRKTPVQQLASPASFSPDILADIFELFAKNFSYGKPLNNEW
                     QLPDPSEIFTCDHTELNAFLDLKNSLNEVKNLLSDKKLDEWHEHTAFTNKAGKIISHV
                     RKSVNAELCTQAWCKFHEILCSFPLIPQEAFQNGKLNSLHLCEAPGAFIASLNHYLKS
                     HRFPCHWSWVANTLNPYHEANDDLMMIMDDRLIANTLHWWYFGPDNTGDIMTLKFLTG
                     LQNFISSMATVHLVTADGSFDCQGNPGEQEALVSSLHYCEVVTALTTLGNGGSFVLKM
                     FTMFEHCSINLMYLLNCCFDQVHVFKPATSKAGNSEVYVVCLHYKGREAIHPLLSKMT
                     LNFGTEMKRKALFPHHVIPDSFLKRHEECCVFFHKYQLETISENIRLFECMGKAEQEK
                     LNNLRDCAIQYFMQKFQLKHLSRNNWLVKKSSIGCSTNTKWFGQRNKYFKTYNERKML
                     EALSWKDKVAKGYFNSWAEEHGVYHPGQSSILEGTASNLECHLWHILEGKKLPKVKCS
                     PFCNGEILKTLNEAIEKSLGGAFNLDSKFRPKQQYSCSCHVFSEELIFSELCSLTECL
                     QDEQVVVPSNQIKCLLVGFSTLRNIKMHIPLEVRLLESAELTTFSCSLLHDGDPTYQR
                     LFLDCLLHSLRELHTGDVMILPVLSCFTRFMAGLIFVLHSCFRFITFVCPTSSDPLRT
                     CAVLLCVGYQDLPNPVFRYLQSVNELLSTLLNSDSPQQVLQFVPMEVLLKGALLDFLW
                     DLNAAIAKRHLHFIIQREREEIINSLQLQN"
     regulatory      3883..3888
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CMTR2"
                     /gene_synonym="AFT; FTSJD1; HMTr2; MTr2"
                     /note="hexamer: AATAAA"
     polyA_site      3900
                     /gene="CMTR2"
                     /gene_synonym="AFT; FTSJD1; HMTr2; MTr2"
                     /note="major polyA site"
     regulatory      3946..3951
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CMTR2"
                     /gene_synonym="AFT; FTSJD1; HMTr2; MTr2"
                     /note="hexamer: AATAAA"
     polyA_site      3967
                     /gene="CMTR2"
                     /gene_synonym="AFT; FTSJD1; HMTr2; MTr2"
                     /note="major polyA site"
     regulatory      4856..4861
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CMTR2"
                     /gene_synonym="AFT; FTSJD1; HMTr2; MTr2"
                     /note="hexamer: ATTAAA"
     polyA_site      4875
                     /gene="CMTR2"
                     /gene_synonym="AFT; FTSJD1; HMTr2; MTr2"
ORIGIN      
        1 gagtgcctcc tggtccctgt ctgccggcat tcgcggctgc ggggcccgga ggtgggactg
       61 gcttcccggt gccgcgaggg cgggtccgga cagccttccc cccagtccgg cgcaccatct
      121 ccctgccttg tggctggagg cgccgcggac ccaaagggag ggaccatccc gggaagcagc
      181 cccgagagcg gaagtgcaga atggcttcct cgagagagta aagtgcagcc tctccagaca
      241 ctggggcccc agtgggcgtg ggcgaagttg ctggtaggag gagttggcgg aagcacttgg
      301 aactccttta taagtgtcag ctgtgagatt ttaatttgat ttgaaaatga gtaagtgcag
      361 aaagacacca gttcagcagc tagcaagtcc cgcgtcattc agcccagata ttcttgctga
      421 catttttgaa ctctttgcca agaacttttc ttatggcaag ccacttaata atgagtggca
      481 gttaccagat cccagtgaga ttttcacctg tgaccacact gaacttaatg catttcttga
      541 tttgaagaac tccctaaatg aagtaaaaaa cctactgagt gataagaaac tggatgagtg
      601 gcatgagcac actgctttca ctaataaagc ggggaaaatc atttctcatg ttagaaaatc
      661 tgtgaatgct gaactttgta ctcaagcatg gtgtaagttc catgagattt tgtgcagctt
      721 tccacttatt ccacaggaag cttttcagaa tggaaaactg aattctctac acctttgtga
      781 agctccagga gcttttatag ctagtctcaa ccactactta aaatcccatc ggtttccttg
      841 tcattggagt tgggtagcga atactctgaa tccataccat gaagcaaatg acgacctcat
      901 gatgattatg gatgaccggc ttattgcaaa taccttgcac tggtggtact ttggtccaga
      961 taacactggt gatatcatga ccctgaaatt cttgactgga cttcagaatt tcataagcag
     1021 catggctact gttcacttgg tcactgcaga tgggagtttt gattgccaag gaaacccagg
     1081 tgaacaagaa gctttagttt cttctttgca ttactgtgaa gttgtcactg ctctgaccac
     1141 tcttggaaac ggtggctctt ttgttctaaa gatgtttact atgtttgaac attgttccat
     1201 aaacttgatg tacctgctaa actgttgttt tgaccaagtc catgttttca aacctgctac
     1261 tagcaaggca ggaaactccg aagtctatgt ggtttgcctc cactataagg ggagagaggc
     1321 catccatcct ctgttatcta agatgacctt gaattttggg actgaaatga aaaggaaagc
     1381 cctttttccc catcatgtga ttcctgattc ttttcttaag agacatgaag aatgttgtgt
     1441 gttctttcat aaatatcagc tagagactat ttctgaaaac attcgtctat ttgagtgcat
     1501 gggaaaggcg gaacaagaaa agctgaataa tttaagggat tgtgctatac aatattttat
     1561 gcaaaaattt caactgaaac atctttccag aaataattgg ctagtaaaaa aatctagtat
     1621 tggttgtagt acaaatacaa aatggtttgg gcagaggaac aaatatttta aaacttataa
     1681 tgaaaggaag atgctagaag ccctttcatg gaaagataaa gtagccaaag gatactttaa
     1741 tagttgggct gaagaacatg gtgtatatca tcctgggcag agttctattt tagaaggaac
     1801 agcttccaat cttgagtgtc acttatggca tattttggag ggaaagaaac tgccaaaggt
     1861 aaaatgttct cctttttgca atggtgaaat tttaaaaact cttaatgaag caattgaaaa
     1921 gtcattagga ggagctttta atttggattc caagtttagg ccaaaacagc agtattcttg
     1981 ttcttgtcat gttttttctg aagaactgat attttccgag ttgtgtagcc ttactgagtg
     2041 ccttcaggat gagcaggttg tagtacccag caatcaaata aagtgcctgc tggtgggctt
     2101 ttcgactctc cgtaatatca aaatgcatat accgttggaa gttcgactcc tagaatcagc
     2161 tgaactcaca acttttagct gttcattgct tcatgatgga gatccaactt accagcgttt
     2221 atttttggac tgccttctac attcattgcg ggagcttcat acaggagatg ttatgatttt
     2281 gcctgtactt tcttgcttca caagatttat ggctggtttg atctttgtac tccacagttg
     2341 ttttagattc atcacttttg tttgtcccac atcctctgat cccctgagga cctgcgcagt
     2401 cctgctatgt gttggttatc aggaccttcc aaatccagtt ttccgatatt tgcagagtgt
     2461 gaatgaattg ttgagcactt tgctcaactc tgactcaccc cagcaggttt tacagtttgt
     2521 gccaatggag gtactcctta agggggccct gcttgatttt ttgtgggatt tgaatgctgc
     2581 cattgctaaa aggcatttgc atttcattat tcaaagagag agagaagaaa ttatcaacag
     2641 ccttcagtta caaaactgaa catatgcttt ctgagattca actttatgat ttcttataat
     2701 ttgcccagta tttgcatcct gttgctctat taatttaaaa accttttatt ttggggaaag
     2761 gccaacattt gcatcattca aagtctcatt aattctggaa aaccatccat tctgatctct
     2821 agggtatata cacccacagg catagagctc ttccacgtgg tggaatctat gcaatgatag
     2881 atattcacac tctaaatatg aggtgtgtgt atgtgtatgg gtggccacag ccatgcttac
     2941 ctatgccatt tagttggtct tacttaatct gcttaagatt tgcatctgtg tacctttgtt
     3001 cagattagtt ttttttttcc agccgatttc ctcttagtgg ctaatgctgt tagtgaattt
     3061 tccaactaat ttcctctcat tggttaatgt tgttaatgaa ttgagagagg taattgagga
     3121 aaggaaatga gtaaatcact gttcagcaac actgatttcc gttaacacat cagttatgaa
     3181 tttcagggaa ttcatctcgc cagattcttg ataacatgcc attcattgcc cttaggtgat
     3241 tgaccctatt ttcttacatg gctcaaataa aactagtatg ctgttgtatg aatcttttac
     3301 tgaccacacc atccaactat aaaaatataa cgggacagct ttaaaccaaa gatcatgttt
     3361 agaacaatga aaaattattt gttgtatcta atacacgcct gtattgtgaa aagcttcatt
     3421 tagcaatgat gtaataattt ttaacttcca ggaaataatc tgtgaatgga aagatttttt
     3481 aagattttga gatagtgttt agtctcatgt tgggaacaca tgaatgtgat gaacatagtg
     3541 aatactaaag aaaacgcttc agactttcag aatgatggtt cagaatttaa aatttttaat
     3601 cttttctaat ttcttttttt cagtgtgaaa atagcacttt accaaaagat tagccatgaa
     3661 atggttattt tgccagttac atttgatttc ttttgtatct gcaatgtaat gagttatttt
     3721 atttcttctg tatttgcagt gtaatgagtt tttgtggcaa agtgtattaa gcaatttttc
     3781 attatcttga agttccacaa agtggagaat atttatattc tcacatgcat tttaggcact
     3841 tttgatatgt gaaaatagat gtattttctg atgcatttgg ttaataaata ttaatctgaa
     3901 cattttcatg ttctttgcta ttttgaattc cattatagat tcatgaataa agtcattact
     3961 agagagattt tgtgttcatc ttttttaaat ggaattatgg gagaagttaa aaatataagt
     4021 tggaaacaga catttttaaa gggagttttg aatgatagtt aatgttcttt cattttctct
     4081 gatttgcatt cattaaaatt tagtccttaa tattgtaata cttatggaag gcctacttca
     4141 ggaaagaaat tgaatactta agggggattg gaagaggagt gtgtggaggc ctggaagtag
     4201 atttttgaaa gaaatctgat ttcctactgg tgactctatg gaagcaattc cagttgtatg
     4261 acgatggaag tctgacagaa ggttgattac caggaggaaa aaaaaaaaca ctcttggaaa
     4321 accattttgc acttaatagt ctttatgcct tgctaagtgg gattcaatat actaataatg
     4381 cagcttattt tttacttatt ttaactattt gataagaatg aagttgaagg catgggtcca
     4441 gattctagca ttattactgt ctttaagccc ttcaaataag tattgaatgc ttagtgtttt
     4501 gttgacacta atctgtactg gaatatataa cactgaagta ttctcaaatg tattttgctt
     4561 tttaacgtgg gttttggaat caattgctca tctgattcat tggattcaaa ttgctttaga
     4621 ggttagacat gacttgagga aggttcagat tgaactggat atattaatag atgacaggta
     4681 ctaatgattt atatgagttt ctctttaaga gaaaggagag gaaaaatgct attcaaaaga
     4741 tgaatggaaa agtagaagaa gacaggaaga ggtgagagta tgcatgtttt accaaggtag
     4801 actgttctta aaaacttttt tccacaggtt ttgagatttt taatttgtaa tatctattaa
     4861 aaaacaaaca tcaaa
//
