LOCUS       NM_138809               3893 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens carboxymethylenebutenolidase homolog (CMBL), mRNA.
ACCESSION   NM_138809
VERSION     NM_138809.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Huang,Y., Xiong,C., Wang,C., Deng,J., Zuo,Z., Wu,H., Xiong,J.,
            Wu,X., Lu,H., Hao,Q. and Zhou,X.
  TITLE     p53-responsive CMBL reprograms glucose metabolism and suppresses
            cancer development by destabilizing phosphofructokinase PFKP
  JOURNAL   Cell Rep 42 (11), 113426 (2023)
   PUBMED   37967006
  REMARK    GeneRIF: p53-responsive CMBL reprograms glucose metabolism and
            suppresses cancer development by destabilizing phosphofructokinase
            PFKP.
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Ishizuka,T., Fujimori,I., Kato,M., Noji-Sakikawa,C., Saito,M.,
            Yoshigae,Y., Kubota,K., Kurihara,A., Izumi,T., Ikeda,T. and
            Okazaki,O.
  TITLE     Human carboxymethylenebutenolidase as a bioactivating hydrolase of
            olmesartan medoxomil in liver and intestine
  JOURNAL   J Biol Chem 285 (16), 11892-11902 (2010)
   PUBMED   20177059
  REMARK    GeneRIF: By comparing the enzyme kinetics and chemical inhibition
            properties between the recombinant protein and tissue preparations,
            CMBL was shown to be the primary olmesartan medoxomil bioactivating
            enzyme in the liver and intestine.
REFERENCE   3  (bases 1 to 3893)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   4  (bases 1 to 3893)
  AUTHORS   Fatima,S., Chui,C.H., Tang,W.K., Hui,K.S., Au,H.W., Li,W.Y.,
            Wong,M.M., Cheung,F., Tsao,S.W., Lam,K.Y., Beh,P.S., Wong,J.,
            Law,S., Srivastava,G., Ho,K.P., Chan,A.S. and Tang,J.C.
  TITLE     Transforming capacity of two novel genes JS-1 and JS-2 located in
            chromosome 5p and their overexpression in human esophageal squamous
            cell carcinoma
  JOURNAL   Int J Mol Med 17 (1), 159-170 (2006)
   PUBMED   16328025
REFERENCE   5  (bases 1 to 3893)
  AUTHORS   Frantz,B., Ngai,K.L., Chatterjee,D.K., Ornston,L.N. and
            Chakrabarty,A.M.
  TITLE     Nucleotide sequence and expression of clcD, a plasmid-borne
            dienelactone hydrolase gene from Pseudomonas sp. strain B13
  JOURNAL   J Bacteriol 169 (2), 704-709 (1987)
   PUBMED   3804974
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA628493.1, BC001573.1 and AC012640.12.
            
            On Nov 23, 2018 this sequence version replaced NM_138809.3.
            
            Summary: CMBL (EC 3.1.1.45) is a cysteine hydrolase of the
            dienelactone hydrolase family that is highly expressed in liver
            cytosol. CMBL preferentially cleaves cyclic esters, and it
            activates medoxomil-ester prodrugs in which the medoxomil moiety is
            linked to an oxygen atom (Ishizuka et al., 2010 [PubMed
            20177059]).[supplied by OMIM, Apr 2010].
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038193.4231738.1, AJ278125.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000296658.4/ ENSP00000296658.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-140               DA628493.1         155-294
            141-1943            BC001573.1         1-1803
            1944-3893           AC012640.12        100222-102171
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5p15.2"
     gene            1..3893
                     /gene="CMBL"
                     /gene_synonym="JS-1"
                     /note="carboxymethylenebutenolidase homolog"
                     /db_xref="GeneID:134147"
                     /db_xref="HGNC:HGNC:25090"
                     /db_xref="MIM:613379"
     exon            1..278
                     /gene="CMBL"
                     /gene_synonym="JS-1"
                     /inference="alignment:Splign:2.1.0"
     exon            279..512
                     /gene="CMBL"
                     /gene_synonym="JS-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             298..1035
                     /gene="CMBL"
                     /gene_synonym="JS-1"
                     /EC_number="3.1.-.-"
                     /note="carboxymethylenebutenolidase-like (Pseudomonas);
                     carboxymethylenebutenolidase homolog (Pseudomonas)"
                     /codon_start=1
                     /product="carboxymethylenebutenolidase homolog"
                     /protein_id="NP_620164.1"
                     /db_xref="CCDS:CCDS3878.1"
                     /db_xref="GeneID:134147"
                     /db_xref="HGNC:HGNC:25090"
                     /db_xref="MIM:613379"
                     /translation="MANEAYPCPCDIGHRLEYGGLGREVQVEHIKAYVTKSPVDAGKA
                     VIVIQDIFGWQLPNTRYIADMISGNGYTTIVPDFFVGQEPWDPSGDWSIFPEWLKTRN
                     AQKIDREISAILKYLKQQCHAQKIGIVGFCWGGTAVHHLMMKYSEFRAGVSVYGIVKD
                     SEDIYNLKNPTLFIFAENDVVIPLKDVSLLTQKLKEHCKVEYQIKTFSGQTHGFVHRK
                     REDCSPADKPYIDEARRNLIEWLNKYM"
     exon            513..620
                     /gene="CMBL"
                     /gene_synonym="JS-1"
                     /inference="alignment:Splign:2.1.0"
     exon            621..763
                     /gene="CMBL"
                     /gene_synonym="JS-1"
                     /inference="alignment:Splign:2.1.0"
     exon            764..855
                     /gene="CMBL"
                     /gene_synonym="JS-1"
                     /inference="alignment:Splign:2.1.0"
     exon            856..3893
                     /gene="CMBL"
                     /gene_synonym="JS-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2187..2192
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CMBL"
                     /gene_synonym="JS-1"
                     /note="hexamer: AATAAA"
     polyA_site      2209
                     /gene="CMBL"
                     /gene_synonym="JS-1"
                     /note="major polyA site"
     regulatory      3288..3293
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CMBL"
                     /gene_synonym="JS-1"
                     /note="hexamer: CATAAA"
     polyA_site      3311
                     /gene="CMBL"
                     /gene_synonym="JS-1"
     regulatory      3875..3880
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CMBL"
                     /gene_synonym="JS-1"
                     /note="hexamer: ATTAAA"
     polyA_site      3893
                     /gene="CMBL"
                     /gene_synonym="JS-1"
ORIGIN      
        1 ctctcgtctc ctccccactg cgcctccctc cctcctggcc ttccttccct tccccgactt
       61 tgcagatttc tcttccccca ggcctccctc ctccacctct ccgccccctc cgggcttggc
      121 tctcccagga ggctacgact ggagccactg gtcccgcagg atccccgcgt cctcggtcgc
      181 cgcgtccacg tccctctcgc gtccccgccc ggcgccacgc cgcctcctct gggttcggcc
      241 tccgcgcggt gcagcgcagt ctcaggccgc gggacaagcc cgacttaaat ctctgcaatg
      301 gctaacgaag cttatccttg tccgtgtgac attggccaca gacttgagta tggagggcta
      361 ggccgtgaag ttcaagtcga gcacatcaag gcttatgtca ccaaatcccc cgttgatgca
      421 ggcaaagctg tgattgtcat tcaagatata tttggctggc agttgcccaa taccagatat
      481 atagctgaca tgatctcagg aaatggatac acaaccattg ttccagactt ctttgtaggg
      541 caagagcctt gggacccctc tggcgactgg tctatcttcc ctgagtggct gaaaacaaga
      601 aatgcccaga agatcgatag agagatcagt gctatcttga agtatctgaa acaacagtgt
      661 catgcccaga aaattggcat cgtgggattc tgctggggtg gaactgctgt ccatcatttg
      721 atgatgaaat actcagaatt cagggcaggg gtgtccgtct atggcattgt caaggattct
      781 gaagacattt acaatttaaa gaaccccact ttgttcattt ttgctgaaaa tgatgttgtg
      841 attccactca aggacgtatc tttgctgact cagaagttga aagaacactg caaagttgaa
      901 tatcaaatta aaacattttc tgggcagact catgggttcg tgcatcggaa gagagaagat
      961 tgctcacctg cagacaagcc ctacattgac gaggccagaa ggaatttaat tgagtggctg
     1021 aacaagtaca tgtagcaaga atcaagggca agccttccta gaatagcttt catcccaaaa
     1081 tttgcttgga aatagttaga tcatttgatt taattttcac ttttataaaa taagtgtagg
     1141 aatcctaaaa ttgattattt catttgaaac acaaattcag taggacgtaa tgcatgaaat
     1201 aatttaattt ttgacatgta catcgaatca taatttaaaa acaaggtctg accaggtgta
     1261 gtgcctcatg cctgtaattc cagcactttg ggaggccaaa gtgggtggat cacctgaggt
     1321 caggagtttg agaccagcct ggccaacatg gtgagacccc atctctacaa aaaatacaaa
     1381 aattagcctg gtgtggtggt gcacgcctgt agtcccagct acttgggagg ctgaggcaca
     1441 agaatcaata gaacccagga ggtggagact gcagtgagcc aagattgtgc cactactgta
     1501 ctctagcctg ggcagcagag tgagaccctg tctcaaaaat aaataagtaa ataaataaat
     1561 aaataaaata aaaaccaggt cagtacctgg agaatttgaa tgatagagaa tgatagagta
     1621 ataccctaat tattagttaa aacctacagg ccgggtgcgg tggctcacgc ctgtaatccc
     1681 agcactttgg gaggccgagg tgggtggatc acttgaggtc aggagttcaa gaccagcctg
     1741 gccaacatgg tgaaacccca tctctactaa aaatataaaa atcagccggg catggtggca
     1801 tgtgcctgta atcccagcta ctcaggagtc tgaggaggag aatcacttga acctggaggc
     1861 agaggttgca gtgagtcgag gttgcgctac tgcactccag cctggacaac agagggagac
     1921 tctgtctcaa aaaaaaaaaa aaacctacag ctgttcaagg accagctgac aggtcaagtg
     1981 tggccttttc tggtctttga acacatcata gaaagtgaca aatgctgcaa agccatgaag
     2041 aacatgaact ataaacgggt agactaactg cccagcttag acacttatct atgccacaaa
     2101 acagctgaat ttgtcacatt tatatattgc aatatgggaa gtattgagat caaaacagga
     2161 ttccattgac ctaattatat taagctaata aactaatttt ttgaattttt gaatttgcag
     2221 cttttttaaa aagatagcag agaacggtgg gctagagttg tcacccagct gttgtctcct
     2281 aagcagtgga tttccaagca ctggcatttt tgggtcccag ggcctgaggc tacctcagaa
     2341 atatctcctg tgtttctggt ggtggcgtaa aggggagagc cggtgagaaa ctctgcacct
     2401 gcacctacgg ggtgcgtaca tgtgactttg ggcaagtgac ttgatgttgt agtttttttc
     2461 ctaattttgt cttgacgtcc ttctcaggag agagtggctg taaactctag ccctgccctg
     2521 acggggatcc tggggatgtg gtcgtgggtg tttcccatgt gcctttcatg ggatccttct
     2581 ttgacctcac agacggcctg attcctgagt gtccaacctg tggccaggtg tccctctcgc
     2641 aggaagcttg tttatcctgg cacatgacct tgtggctctc atctgacctg tgtccagttt
     2701 attcctactg agacagccac tctcccggag agccctgacc gggaaagaag ttaggatggg
     2761 gtagaggagg caactcatca gaacccaaga aatgacagaa gcagtttatt gcttacagat
     2821 ccagagaaca gagggcagca cgcttcacag ggccagcaaa aagggggagc cattcaggac
     2881 acatgggctc aaccagaagg tggagaacga gagagagagg gagctatgag ccaaagcctt
     2941 cactggggcc cagggtgtca tctatgcagg tttcccctgg aggttctaac tggggggttt
     3001 agagcaagca ggcatgagcc ccggggactc acgctgtgac tgagaggggt cggtgtgggt
     3061 aggatatcgg catggtccat gtgggtgtgg gggtcagtgg gacaagtcaa gcaggtggta
     3121 tccagctgtc catgggaagg tggtcccagg aggcggtttt gtaaggcaga tgtctggatc
     3181 gaccaccttg gggaacagtg agaagttgta taactggaaa ctgtgttact aatccctgct
     3241 tctagtatga gaaagtccaa cttacattca aatagatgct caggaaacat aaaattcaaa
     3301 gaattcccaa acttgagctt cctgagcctc agtttttgca tctgtcaaat gggtatgggg
     3361 agaccctttg cctaatgttg agagaatcca agcctgagca gggctggtct actgtggaca
     3421 cttagttgtg gaccccaaca ggccaaagcc cttgcctgct gagggctgct cccaggaggc
     3481 ctcacacatt catccggcct cccaggaaac ccctgagatg tgctgggctt ttgccgtgga
     3541 acggtgtcgg tgagcatgtg gccagtagcc agagtcttca acataggtgg gaggtctgaa
     3601 attcgtgcga gaatgaacca agggtgtggc tgtatccttc atggtgagct tggggcctgg
     3661 gcagccttcc ctctgctgag tcctttggtc aaatgtcatg gtgttctgaa ccagcactgc
     3721 agatagaagt ataatgtaag tcacacacgt acttctttaa aaagatatgt tcttcagtga
     3781 ctattagaca aacagataaa tgaaggtaaa gggaaaaaat ggcaacttta gcttttttcc
     3841 ccatgtatgt aattttaaat tttcttatag ccccattaaa aaaaggaaag aga
//
