LOCUS       NM_001831               2749 bp    mRNA    linear   PRI 17-SEP-2024
DEFINITION  Homo sapiens clusterin (CLU), transcript variant 1, mRNA.
ACCESSION   NM_001831
VERSION     NM_001831.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Chen,C., Shu,Y., Yan,C., Li,H., Huang,Z., Shen,S., Liu,C.,
            Jiang,Y., Huang,S., Wang,Z., Mei,F., Qin,F., Liu,X. and Qiu,W.
  TITLE     Astrocyte-derived clusterin disrupts glial physiology to obstruct
            remyelination in mouse models of demyelinating diseases
  JOURNAL   Nat Commun 15 (1), 7791 (2024)
   PUBMED   39242637
  REMARK    GeneRIF: Astrocyte-derived clusterin disrupts glial physiology to
            obstruct remyelination in mouse models of demyelinating diseases.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Mamun,M., Zheng,Y.C., Wang,N., Wang,B., Zhang,Y., Pang,J.R.,
            Shen,D.D., Liu,H.M. and Gao,Y.
  TITLE     Decoding CLU (Clusterin): Conquering cancer treatment resistance
            and immunological barriers
  JOURNAL   Int Immunopharmacol 137, 112355 (2024)
   PUBMED   38851158
  REMARK    GeneRIF: Decoding CLU (Clusterin): Conquering cancer treatment
            resistance and immunological barriers.
            Review article
REFERENCE   3  (bases 1 to 2749)
  AUTHORS   Smith,A.S., Subramanian,J., Doderer,J. and Moskovitz,J.
  TITLE     Methionine oxidation of clusterin in Alzheimer's disease and its
            effect on clusterin's binding to beta-amyloid
  JOURNAL   Neurosci Lett 836, 137874 (2024)
   PUBMED   38857696
  REMARK    GeneRIF: Methionine oxidation of clusterin in Alzheimer's disease
            and its effect on clusterin's binding to beta-amyloid.
REFERENCE   4  (bases 1 to 2749)
  AUTHORS   Wong,P., Taillefer,D., Lakins,J., Pineault,J., Chader,G. and
            Tenniswood,M.
  TITLE     Molecular characterization of human TRPM-2/clusterin, a gene
            associated with sperm maturation, apoptosis and neurodegeneration
  JOURNAL   Eur J Biochem 221 (3), 917-925 (1994)
   PUBMED   8181474
REFERENCE   5  (bases 1 to 2749)
  AUTHORS   Fink,T.M., Zimmer,M., Tschopp,J., Etienne,J., Jenne,D.E. and
            Lichter,P.
  TITLE     Human clusterin (CLI) maps to 8p21 in proximity to the lipoprotein
            lipase (LPL) gene
  JOURNAL   Genomics 16 (2), 526-528 (1993)
   PUBMED   8314591
REFERENCE   6  (bases 1 to 2749)
  AUTHORS   Choi-Miura,N.H., Takahashi,Y., Nakano,Y., Tobe,T. and Tomita,M.
  TITLE     Identification of the disulfide bonds in human plasma protein
            SP-40,40 (apolipoprotein-J)
  JOURNAL   J Biochem 112 (4), 557-561 (1992)
   PUBMED   1491011
REFERENCE   7  (bases 1 to 2749)
  AUTHORS   Kirszbaum,L., Bozas,S.E. and Walker,I.D.
  TITLE     SP-40,40, a protein involved in the control of the complement
            pathway, possesses a unique array of disulphide bridges
  JOURNAL   FEBS Lett 297 (1-2), 70-76 (1992)
   PUBMED   1551440
REFERENCE   8  (bases 1 to 2749)
  AUTHORS   Dietzsch,E., Murphy,B.F., Kirszbaum,L., Walker,I.D. and Garson,O.M.
  TITLE     Regional localization of the gene for clusterin (SP-40,40; gene
            symbol CLI) to human chromosome 8p12-->p21
  JOURNAL   Cytogenet Cell Genet 61 (3), 178-179 (1992)
   PUBMED   1424805
REFERENCE   9  (bases 1 to 2749)
  AUTHORS   de Silva,H.V., Stuart,W.D., Park,Y.B., Mao,S.J., Gil,C.M.,
            Wetterau,J.R., Busch,S.J. and Harmony,J.A.
  TITLE     Purification and characterization of apolipoprotein J
  JOURNAL   J Biol Chem 265 (24), 14292-14297 (1990)
   PUBMED   2387851
REFERENCE   10 (bases 1 to 2749)
  AUTHORS   O'Bryan,M.K., Baker,H.W., Saunders,J.R., Kirszbaum,L., Walker,I.D.,
            Hudson,P., Liu,D.Y., Glew,M.D., d'Apice,A.J. and Murphy,B.F.
  TITLE     Human seminal clusterin (SP-40,40). Isolation and characterization
  JOURNAL   J Clin Invest 85 (5), 1477-1486 (1990)
   PUBMED   2185274
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DN990822.1, BC010514.1,
            AF311103.5 and BM682289.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_001831.3.
            
            Summary: The protein encoded by this gene is a secreted chaperone
            that can under some stress conditions also be found in the cell
            cytosol. It has been suggested to be involved in several basic
            biological events such as cell death, tumor progression, and
            neurodegenerative disorders. Alternate splicing results in both
            coding and non-coding variants.[provided by RefSeq, May 2011].
            
            Transcript Variant: This variant (1) encodes the functional
            protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.272373.1,
                                           SRR1660809.146328.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000316403.15/ ENSP00000315130.10
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-7                 DN990822.1         120-126
            8-863               BC010514.1         1-856
            864-864             AF311103.5         214847-214847       c
            865-1670            BC010514.1         858-1663
            1671-2463           AF311103.5         207086-207878       c
            2464-2749           BM682289.1         18-303              c
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p21.1"
     gene            1..2749
                     /gene="CLU"
                     /gene_synonym="AAG4; APO-J; APOJ; CLI; CLU1; CLU2; KUB1;
                     NA1/NA2; SGP-2; SGP2; SP-40; TRPM-2; TRPM2"
                     /note="clusterin"
                     /db_xref="GeneID:1191"
                     /db_xref="HGNC:HGNC:2095"
                     /db_xref="MIM:185430"
     exon            1..46
                     /gene="CLU"
                     /gene_synonym="AAG4; APO-J; APOJ; CLI; CLU1; CLU2; KUB1;
                     NA1/NA2; SGP-2; SGP2; SP-40; TRPM-2; TRPM2"
                     /inference="alignment:Splign:2.1.0"
     exon            47..172
                     /gene="CLU"
                     /gene_synonym="AAG4; APO-J; APOJ; CLI; CLU1; CLU2; KUB1;
                     NA1/NA2; SGP-2; SGP2; SP-40; TRPM-2; TRPM2"
                     /inference="alignment:Splign:2.1.0"
     CDS             76..1425
                     /gene="CLU"
                     /gene_synonym="AAG4; APO-J; APOJ; CLI; CLU1; CLU2; KUB1;
                     NA1/NA2; SGP-2; SGP2; SP-40; TRPM-2; TRPM2"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; complement lysis inhibitor;
                     testosterone-repressed prostate message 2;
                     aging-associated protein 4; sulfated glycoprotein 2;
                     apolipoprotein J; ku70-binding protein 1; complement
                     cytolysis inhibitor; complement-associated protein
                     SP-40,40; epididymis secretory sperm binding protein"
                     /codon_start=1
                     /product="clusterin isoform 1 preproprotein"
                     /protein_id="NP_001822.3"
                     /db_xref="CCDS:CCDS47832.1"
                     /db_xref="GeneID:1191"
                     /db_xref="HGNC:HGNC:2095"
                     /db_xref="MIM:185430"
                     /translation="MMKTLLLFVGLLLTWESGQVLGDQTVSDNELQEMSNQGSKYVNK
                     EIQNAVNGVKQIKTLIEKTNEERKTLLSNLEEAKKKKEDALNETRESETKLKELPGVC
                     NETMMALWEECKPCLKQTCMKFYARVCRSGSGLVGRQLEEFLNQSSPFYFWMNGDRID
                     SLLENDRQQTHMLDVMQDHFSRASSIIDELFQDRFFTREPQDTYHYLPFSLPHRRPHF
                     FFPKSRIVRSLMPFSPYEPLNFHAMFQPFLEMIHEAQQAMDIHFHSPAFQHPPTEFIR
                     EGDDDRTVCREIRHNSTGCLRMKDQCDKCREILSVDCSTNNPSQAKLRRELDESLQVA
                     ERLTRKYNELLKSYQWKMLNTSSLLEQLNEQFNWVSRLANLTQGEDQYYLRVTTVASH
                     TSDSDVPSGVTEVVVKLFDSDPITVTVPVEVSRKNPKFMETVAEKALQEYRKKHREE"
     exon            173..321
                     /gene="CLU"
                     /gene_synonym="AAG4; APO-J; APOJ; CLI; CLU1; CLU2; KUB1;
                     NA1/NA2; SGP-2; SGP2; SP-40; TRPM-2; TRPM2"
                     /inference="alignment:Splign:2.1.0"
     exon            322..492
                     /gene="CLU"
                     /gene_synonym="AAG4; APO-J; APOJ; CLI; CLU1; CLU2; KUB1;
                     NA1/NA2; SGP-2; SGP2; SP-40; TRPM-2; TRPM2"
                     /inference="alignment:Splign:2.1.0"
     exon            493..904
                     /gene="CLU"
                     /gene_synonym="AAG4; APO-J; APOJ; CLI; CLU1; CLU2; KUB1;
                     NA1/NA2; SGP-2; SGP2; SP-40; TRPM-2; TRPM2"
                     /inference="alignment:Splign:2.1.0"
     exon            905..1009
                     /gene="CLU"
                     /gene_synonym="AAG4; APO-J; APOJ; CLI; CLU1; CLU2; KUB1;
                     NA1/NA2; SGP-2; SGP2; SP-40; TRPM-2; TRPM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1010..1239
                     /gene="CLU"
                     /gene_synonym="AAG4; APO-J; APOJ; CLI; CLU1; CLU2; KUB1;
                     NA1/NA2; SGP-2; SGP2; SP-40; TRPM-2; TRPM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1240..1415
                     /gene="CLU"
                     /gene_synonym="AAG4; APO-J; APOJ; CLI; CLU1; CLU2; KUB1;
                     NA1/NA2; SGP-2; SGP2; SP-40; TRPM-2; TRPM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1416..2749
                     /gene="CLU"
                     /gene_synonym="AAG4; APO-J; APOJ; CLI; CLU1; CLU2; KUB1;
                     NA1/NA2; SGP-2; SGP2; SP-40; TRPM-2; TRPM2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1648..1653
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLU"
                     /gene_synonym="AAG4; APO-J; APOJ; CLI; CLU1; CLU2; KUB1;
                     NA1/NA2; SGP-2; SGP2; SP-40; TRPM-2; TRPM2"
                     /note="hexamer: AATAAA"
     polyA_site      1670
                     /gene="CLU"
                     /gene_synonym="AAG4; APO-J; APOJ; CLI; CLU1; CLU2; KUB1;
                     NA1/NA2; SGP-2; SGP2; SP-40; TRPM-2; TRPM2"
                     /note="major polyA site"
     regulatory      1919..1924
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLU"
                     /gene_synonym="AAG4; APO-J; APOJ; CLI; CLU1; CLU2; KUB1;
                     NA1/NA2; SGP-2; SGP2; SP-40; TRPM-2; TRPM2"
                     /note="hexamer: AATATA"
     polyA_site      1939
                     /gene="CLU"
                     /gene_synonym="AAG4; APO-J; APOJ; CLI; CLU1; CLU2; KUB1;
                     NA1/NA2; SGP-2; SGP2; SP-40; TRPM-2; TRPM2"
     regulatory      1955..1960
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLU"
                     /gene_synonym="AAG4; APO-J; APOJ; CLI; CLU1; CLU2; KUB1;
                     NA1/NA2; SGP-2; SGP2; SP-40; TRPM-2; TRPM2"
                     /note="hexamer: TATAAA"
     polyA_site      1978
                     /gene="CLU"
                     /gene_synonym="AAG4; APO-J; APOJ; CLI; CLU1; CLU2; KUB1;
                     NA1/NA2; SGP-2; SGP2; SP-40; TRPM-2; TRPM2"
     regulatory      2004..2009
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLU"
                     /gene_synonym="AAG4; APO-J; APOJ; CLI; CLU1; CLU2; KUB1;
                     NA1/NA2; SGP-2; SGP2; SP-40; TRPM-2; TRPM2"
                     /note="hexamer: AATACA"
     polyA_site      2029
                     /gene="CLU"
                     /gene_synonym="AAG4; APO-J; APOJ; CLI; CLU1; CLU2; KUB1;
                     NA1/NA2; SGP-2; SGP2; SP-40; TRPM-2; TRPM2"
     polyA_site      2133
                     /gene="CLU"
                     /gene_synonym="AAG4; APO-J; APOJ; CLI; CLU1; CLU2; KUB1;
                     NA1/NA2; SGP-2; SGP2; SP-40; TRPM-2; TRPM2"
     polyA_site      2732
                     /gene="CLU"
                     /gene_synonym="AAG4; APO-J; APOJ; CLI; CLU1; CLU2; KUB1;
                     NA1/NA2; SGP-2; SGP2; SP-40; TRPM-2; TRPM2"
     polyA_site      2749
                     /gene="CLU"
                     /gene_synonym="AAG4; APO-J; APOJ; CLI; CLU1; CLU2; KUB1;
                     NA1/NA2; SGP-2; SGP2; SP-40; TRPM-2; TRPM2"
ORIGIN      
        1 gcggcgtcgc caggaggagc gcgcgggcac agggtgccgc tgaccgaggc gtgcaaagac
       61 tccagaattg gaggcatgat gaagactctg ctgctgtttg tggggctgct gctgacctgg
      121 gagagtgggc aggtcctggg ggaccagacg gtctcagaca atgagctcca ggaaatgtcc
      181 aatcagggaa gtaagtacgt caataaggaa attcaaaatg ctgtcaacgg ggtgaaacag
      241 ataaagactc tcatagaaaa aacaaacgaa gagcgcaaga cactgctcag caacctagaa
      301 gaagccaaga agaagaaaga ggatgcccta aatgagacca gggaatcaga gacaaagctg
      361 aaggagctcc caggagtgtg caatgagacc atgatggccc tctgggaaga gtgtaagccc
      421 tgcctgaaac agacctgcat gaagttctac gcacgcgtct gcagaagtgg ctcaggcctg
      481 gttggccgcc agcttgagga gttcctgaac cagagctcgc ccttctactt ctggatgaat
      541 ggtgaccgca tcgactccct gctggagaac gaccggcagc agacgcacat gctggatgtc
      601 atgcaggacc acttcagccg cgcgtccagc atcatagacg agctcttcca ggacaggttc
      661 ttcacccggg agccccagga tacctaccac tacctgccct tcagcctgcc ccaccggagg
      721 cctcacttct tctttcccaa gtcccgcatc gtccgcagct tgatgccctt ctctccgtac
      781 gagcccctga acttccacgc catgttccag cccttccttg agatgataca cgaggctcag
      841 caggccatgg acatccactt ccatagcccg gccttccagc acccgccaac agaattcata
      901 cgagaaggcg acgatgaccg gactgtgtgc cgggagatcc gccacaactc cacgggctgc
      961 ctgcggatga aggaccagtg tgacaagtgc cgggagatct tgtctgtgga ctgttccacc
     1021 aacaacccct cccaggctaa gctgcggcgg gagctcgacg aatccctcca ggtcgctgag
     1081 aggttgacca ggaaatacaa cgagctgcta aagtcctacc agtggaagat gctcaacacc
     1141 tcctccttgc tggagcagct gaacgagcag tttaactggg tgtcccggct ggcaaacctc
     1201 acgcaaggcg aagaccagta ctatctgcgg gtcaccacgg tggcttccca cacttctgac
     1261 tcggacgttc cttccggtgt cactgaggtg gtcgtgaagc tctttgactc tgatcccatc
     1321 actgtgacgg tccctgtaga agtctccagg aagaacccta aatttatgga gaccgtggcg
     1381 gagaaagcgc tgcaggaata ccgcaaaaag caccgggagg agtgagatgt ggatgttgct
     1441 tttgcaccta cgggggcatc tgagtccagc tccccccaag atgagctgca gccccccaga
     1501 gagagctctg cacgtcacca agtaaccagg ccccagcctc caggccccca actccgccca
     1561 gcctctcccc gctctggatc ctgcactcta acactcgact ctgctgctca tgggaagaac
     1621 agaattgctc ctgcatgcaa ctaattcaat aaaactgtct tgtgagctga tcgcttggag
     1681 ggtcctcttt ttatgttgag ttgctgcttc ccggcatgcc ttcattttgc tatggggggc
     1741 aggcaggggg gatggaaaat aagtagaaac aaaaaagcag tggctaagat ggtataggga
     1801 ctgtcatacc agtgaagaat aaaagggtga agaataaaag ggatatgatg acaaggttga
     1861 tccacttcaa gaattgcttg ctttcaggaa gagagatgtg tttcaacaag ccaactaaaa
     1921 tatattgctg caaatggaag cttttctgtt ctattataaa actgtcgatg tattctgacc
     1981 aaggtgcgac aatctcctaa aggaatacac tgaaagttaa ggagaagaat cagtaagtgt
     2041 aaggtgtact tggtattata atgcataatt gatgttttcg ttatgaaaac atttggtgcc
     2101 cagaagtcca aattatcagt tttatttgta agagctattg cttttgcagc ggttttattt
     2161 gtaaaagctg ttgatttcga gttgtaagag ctcagcatcc caggggcatc ttcttgactg
     2221 tggcatttcc tgtccaccgc cggtttatat gatcttcata cctttccctg gaccacaggc
     2281 gtttctcggc ttttagtctg aaccatagct gggctgcagt accctacgct gccagcaggt
     2341 ggccatgact acccgtggta ccaatctcag tcttaaagct caggcttttc gttcattaac
     2401 attctctgat agaattctgg tcatcagatg tactgcaatg gaacaaaact catctggctg
     2461 catcccaggt gtgtagcaaa gtccacatgt aaatttatag cttagaatat tcttaagtca
     2521 ctgtcccttg tctctctttg aagttataaa caacaaactt aaagcttagc ttatgtccaa
     2581 ggtaagtatt ttagcatggc tgtcaaggaa attcagagta aagtcagtgt gattcactta
     2641 atgatataca ttaattagaa ttatggggtc agaggtattt gcttaagtga tcataattgt
     2701 aaagtatatg tcacattgtc acattaatgt cacactgttt caaaagtta
//
