LOCUS       NM_022131              14202 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens calsyntenin 2 (CLSTN2), mRNA.
ACCESSION   NM_022131
VERSION     NM_022131.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 14202)
  AUTHORS   Schachtl-Riess,J.F., Schonherr,S., Lamina,C., Forer,L., Coassin,S.,
            Streiter,G., Kheirkhah,A., Li,Y., Meiselbach,H., Di Maio,S.,
            Eckardt,K.U., Kottgen,A. and Kronenberg,F.
  CONSRTM   GCKD investigators
  TITLE     KLKB1 and CLSTN2 are associated with HDL-mediated cholesterol
            efflux capacity in a genome-wide association study
  JOURNAL   Atherosclerosis 368, 1-11 (2023)
   PUBMED   36812656
  REMARK    GeneRIF: KLKB1 and CLSTN2 are associated with HDL-mediated
            cholesterol efflux capacity in a genome-wide association study.
REFERENCE   2  (bases 1 to 14202)
  AUTHORS   Subramanian,V.S., Teafatiller,T., Vidal,J., Gunaratne,G.S.,
            Rodriguez-Ortiz,C.J., Kitazawa,M. and Marchant,J.S.
  TITLE     Calsyntenin-3 interacts with the sodium-dependent vitamin C
            transporter-2 to regulate vitamin C uptake
  JOURNAL   Int J Biol Macromol 192, 1178-1184 (2021)
   PUBMED   34673103
  REMARK    GeneRIF: Calsyntenin-3 interacts with the sodium-dependent vitamin
            C transporter-2 to regulate vitamin C uptake.
REFERENCE   3  (bases 1 to 14202)
  AUTHORS   Boraxbekk,C.J., Ames,D., Kochan,N.A., Lee,T., Thalamuthu,A.,
            Wen,W., Armstrong,N.J., Kwok,J.B., Schofield,P.R., Reppermund,S.,
            Wright,M.J., Trollor,J.N., Brodaty,H., Sachdev,P. and Mather,K.A.
  TITLE     Investigating the influence of KIBRA and CLSTN2 genetic
            polymorphisms on cross-sectional and longitudinal measures of
            memory performance and hippocampal volume in older individuals
  JOURNAL   Neuropsychologia 78, 10-17 (2015)
   PUBMED   26415670
  REMARK    GeneRIF: Study did not find support for an association of KIBRA
            either alone or in combination with CLSTN2 with memory performance
            or hippocampal volume, nor did variation in these genes influence
            longitudinal memory decline or hippocampal atrophy in older adults
REFERENCE   4  (bases 1 to 14202)
  AUTHORS   Li,Q., Wojciechowski,R., Simpson,C.L., Hysi,P.G., Verhoeven,V.J.,
            Ikram,M.K., Hohn,R., Vitart,V., Hewitt,A.W., Oexle,K., Makela,K.M.,
            MacGregor,S., Pirastu,M., Fan,Q., Cheng,C.Y., St Pourcain,B.,
            McMahon,G., Kemp,J.P., Northstone,K., Rahi,J.S., Cumberland,P.M.,
            Martin,N.G., Sanfilippo,P.G., Lu,Y., Wang,Y.X., Hayward,C.,
            Polasek,O., Campbell,H., Bencic,G., Wright,A.F., Wedenoja,J.,
            Zeller,T., Schillert,A., Mirshahi,A., Lackner,K., Yip,S.P.,
            Yap,M.K., Ried,J.S., Gieger,C., Murgia,F., Wilson,J.F., Fleck,B.,
            Yazar,S., Vingerling,J.R., Hofman,A., Uitterlinden,A.,
            Rivadeneira,F., Amin,N., Karssen,L., Oostra,B.A., Zhou,X.,
            Teo,Y.Y., Tai,E.S., Vithana,E., Barathi,V., Zheng,Y., Siantar,R.G.,
            Neelam,K., Shin,Y., Lam,J., Yonova-Doing,E., Venturini,C.,
            Hosseini,S.M., Wong,H.S., Lehtimaki,T., Kahonen,M., Raitakari,O.,
            Timpson,N.J., Evans,D.M., Khor,C.C., Aung,T., Young,T.L.,
            Mitchell,P., Klein,B., van Duijn,C.M., Meitinger,T., Jonas,J.B.,
            Baird,P.N., Mackey,D.A., Wong,T.Y., Saw,S.M., Parssinen,O.,
            Stambolian,D., Hammond,C.J., Klaver,C.C., Williams,C.,
            Paterson,A.D., Bailey-Wilson,J.E. and Guggenheim,J.A.
  CONSRTM   CREAM Consortium
  TITLE     Genome-wide association study for refractive astigmatism reveals
            genetic co-determination with spherical equivalent refractive
            error: the CREAM consortium
  JOURNAL   Hum Genet 134 (2), 131-146 (2015)
   PUBMED   25367360
REFERENCE   5  (bases 1 to 14202)
  AUTHORS   Pantzar,A., Laukka,E.J., Atti,A.R., Papenberg,G., Keller,L.,
            Graff,C., Fratiglioni,L. and Backman,L.
  TITLE     Interactive effects of KIBRA and CLSTN2 polymorphisms on episodic
            memory in old-age unipolar depression
  JOURNAL   Neuropsychologia 62, 137-142 (2014)
   PUBMED   25080189
  REMARK    GeneRIF: The results of this study supports the view that effects
            of KIBRA and CLSTN2 polymorphisms genetic polymorphisms on
            cognitive functioning may be most easily disclosed at suboptimal
            levels of cognitive ability, such as in old-age depression.
REFERENCE   6  (bases 1 to 14202)
  AUTHORS   Jacobsen,L.K., Picciotto,M.R., Heath,C.J., Mencl,W.E. and
            Gelernter,J.
  TITLE     Allelic variation of calsyntenin 2 (CLSTN2) modulates the impact of
            developmental tobacco smoke exposure on mnemonic processing in
            adolescents
  JOURNAL   Biol Psychiatry 65 (8), 671-679 (2009)
   PUBMED   19058786
  REMARK    GeneRIF: calsyntenins play an essential role in learning and this
            role is modulated both by CLSTN2 genotype and, during adolescent
            development, by exposure to tobacco smoke.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 14202)
  AUTHORS   Uhl,G.R., Liu,Q.R., Drgon,T., Johnson,C., Walther,D., Rose,J.E.,
            David,S.P., Niaura,R. and Lerman,C.
  TITLE     Molecular genetics of successful smoking cessation: convergent
            genome-wide association study results
  JOURNAL   Arch Gen Psychiatry 65 (6), 683-693 (2008)
   PUBMED   18519826
  REMARK    GeneRIF: Clinical trial and genome-wide association study of
            gene-disease association, gene-environment interaction, and
            pharmacogenomic / toxicogenomic. (HuGE Navigator)
REFERENCE   8  (bases 1 to 14202)
  AUTHORS   Araki,Y., Miyagi,N., Kato,N., Yoshida,T., Wada,S., Nishimura,M.,
            Komano,H., Yamamoto,T., De Strooper,B., Yamamoto,K. and Suzuki,T.
  TITLE     Coordinated metabolism of Alcadein and amyloid beta-protein
            precursor regulates FE65-dependent gene transactivation
  JOURNAL   J Biol Chem 279 (23), 24343-24354 (2004)
   PUBMED   15037614
  REMARK    GeneRIF: Alcadein and amyloid beta-protein precursor regulates
            FE65-dependent gene transactivation [alcalpha1, alcbeta, alcgamma]
REFERENCE   9  (bases 1 to 14202)
  AUTHORS   Araki,Y., Tomita,S., Yamaguchi,H., Miyagi,N., Sumioka,A., Kirino,Y.
            and Suzuki,T.
  TITLE     Novel cadherin-related membrane proteins, Alcadeins, enhance the
            X11-like protein-mediated stabilization of amyloid beta-protein
            precursor metabolism
  JOURNAL   J Biol Chem 278 (49), 49448-49458 (2003)
   PUBMED   12972431
REFERENCE   10 (bases 1 to 14202)
  AUTHORS   Hintsch,G., Zurlinden,A., Meskenaite,V., Steuble,M.,
            Fink-Widmer,K., Kinter,J. and Sonderegger,P.
  TITLE     The calsyntenins--a family of postsynaptic membrane proteins with
            distinct neuronal expression patterns
  JOURNAL   Mol Cell Neurosci 21 (3), 393-409 (2002)
   PUBMED   12498782
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK315308.1, AJ278018.1, BC103506.1, BC004871.2 and AC048346.13.
            
            On Nov 22, 2018 this sequence version replaced NM_022131.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK315308.1, SRR1660807.44270.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000458420.7/ ENSP00000402460.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-180               AK315308.1         1-180
            181-1152            AJ278018.1         1-972
            1153-2472           BC103506.1         972-2291
            2473-4601           AJ278018.1         2293-4421
            4602-4882           BC004871.2         2264-2544
            4883-14202          AC048346.13        52596-61915         c
FEATURES             Location/Qualifiers
     source          1..14202
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q23"
     gene            1..14202
                     /gene="CLSTN2"
                     /gene_synonym="ALC-GAMMA; alcagamma; CDHR13; CS2; CSTN2"
                     /note="calsyntenin 2"
                     /db_xref="GeneID:64084"
                     /db_xref="HGNC:HGNC:17448"
                     /db_xref="MIM:611323"
     exon            1..299
                     /gene="CLSTN2"
                     /gene_synonym="ALC-GAMMA; alcagamma; CDHR13; CS2; CSTN2"
                     /inference="alignment:Splign:2.1.0"
     CDS             191..3058
                     /gene="CLSTN2"
                     /gene_synonym="ALC-GAMMA; alcagamma; CDHR13; CS2; CSTN2"
                     /note="cadherin-related family member 13; alcadein gamma"
                     /codon_start=1
                     /product="calsyntenin-2 precursor"
                     /protein_id="NP_071414.2"
                     /db_xref="CCDS:CCDS3112.1"
                     /db_xref="GeneID:64084"
                     /db_xref="HGNC:HGNC:17448"
                     /db_xref="MIM:611323"
                     /translation="MLPGRLCWVPLLLALGVGSGSGGGGDSRQRRLLAAKVNKHKPWI
                     ETSYHGVITENNDTVILDPPLVALDKDAPVPFAGEICAFKIHGQELPFEAVVLNKTSG
                     EGRLRAKSPIDCELQKEYTFIIQAYDCGAGPHETAWKKSHKAVVHIQVKDVNEFAPTF
                     KEPAYKAVVTEGKIYDSILQVEAIDEDCSPQYSQICNYEIVTTDVPFAIDRNGNIRNT
                     EKLSYDKQHQYEILVTAYDCGQKPAAQDTLVQVDVKPVCKPGWQDWTKRIEYQPGSGS
                     MPLFPSIHLETCDGAVSSLQIVTELQTNYIGKGCDRETYSEKSLQKLCGASSGIIDLL
                     PSPSAATNWTAGLLVDSSEMIFKFDGRQGAKVPDGIVPKNLTDQFTITMWMKHGPSPG
                     VRAEKETILCNSDKTEMNRHHYALYVHNCRLVFLLRKDFDQADTFRPAEFHWKLDQIC
                     DKEWHYYVINVEFPVVTLYMDGATYEPYLVTNDWPIHPSHIAMQLTVGACWQGGEVTK
                     PQFAQFFHGSLASLTIRPGKMESQKVISCLQACKEGLDINSLESLGQGIKYHFNPSQS
                     ILVMEGDDIGNINRALQKVSYINSRQFPTAGVRRLKVSSKVQCFGEDVCISIPEVDAY
                     VMVLQAIEPRITLRGTDHFWRPAAQFESARGVTLFPDIKIVSTFAKTEAPGDVKTTDP
                     KSEVLEEMLHNLDFCDILVIGGDLDPRQECLELNHSELHQRHLDATNSTAGYSIYGVG
                     SMSRYEQVLHHIRYRNWRPASLEARRFRIKCSELNGRYTSNEFNLEVSILHEDQVSDK
                     EHVNHLIVQPPFLQSVHHPESRSSIQHSSVVPSIATVVIIISVCMLVFVVAMGVYRVR
                     IAHQHFIQETEAAKESEMDWDDSALTITVNPMEKHEGPGHGEDETEGEEEEEAEEEMS
                     SSSGSDDSEEEEEEEGMGRGRHGQNGARQAQLEWDDSTLPY"
     exon            300..422
                     /gene="CLSTN2"
                     /gene_synonym="ALC-GAMMA; alcagamma; CDHR13; CS2; CSTN2"
                     /inference="alignment:Splign:2.1.0"
     exon            423..618
                     /gene="CLSTN2"
                     /gene_synonym="ALC-GAMMA; alcagamma; CDHR13; CS2; CSTN2"
                     /inference="alignment:Splign:2.1.0"
     exon            619..827
                     /gene="CLSTN2"
                     /gene_synonym="ALC-GAMMA; alcagamma; CDHR13; CS2; CSTN2"
                     /inference="alignment:Splign:2.1.0"
     exon            828..977
                     /gene="CLSTN2"
                     /gene_synonym="ALC-GAMMA; alcagamma; CDHR13; CS2; CSTN2"
                     /inference="alignment:Splign:2.1.0"
     exon            978..1163
                     /gene="CLSTN2"
                     /gene_synonym="ALC-GAMMA; alcagamma; CDHR13; CS2; CSTN2"
                     /inference="alignment:Splign:2.1.0"
     exon            1164..1412
                     /gene="CLSTN2"
                     /gene_synonym="ALC-GAMMA; alcagamma; CDHR13; CS2; CSTN2"
                     /inference="alignment:Splign:2.1.0"
     exon            1413..1534
                     /gene="CLSTN2"
                     /gene_synonym="ALC-GAMMA; alcagamma; CDHR13; CS2; CSTN2"
                     /inference="alignment:Splign:2.1.0"
     exon            1535..1697
                     /gene="CLSTN2"
                     /gene_synonym="ALC-GAMMA; alcagamma; CDHR13; CS2; CSTN2"
                     /inference="alignment:Splign:2.1.0"
     exon            1698..1864
                     /gene="CLSTN2"
                     /gene_synonym="ALC-GAMMA; alcagamma; CDHR13; CS2; CSTN2"
                     /inference="alignment:Splign:2.1.0"
     exon            1865..2013
                     /gene="CLSTN2"
                     /gene_synonym="ALC-GAMMA; alcagamma; CDHR13; CS2; CSTN2"
                     /inference="alignment:Splign:2.1.0"
     exon            2014..2231
                     /gene="CLSTN2"
                     /gene_synonym="ALC-GAMMA; alcagamma; CDHR13; CS2; CSTN2"
                     /inference="alignment:Splign:2.1.0"
     exon            2232..2402
                     /gene="CLSTN2"
                     /gene_synonym="ALC-GAMMA; alcagamma; CDHR13; CS2; CSTN2"
                     /inference="alignment:Splign:2.1.0"
     exon            2403..2548
                     /gene="CLSTN2"
                     /gene_synonym="ALC-GAMMA; alcagamma; CDHR13; CS2; CSTN2"
                     /inference="alignment:Splign:2.1.0"
     exon            2549..2672
                     /gene="CLSTN2"
                     /gene_synonym="ALC-GAMMA; alcagamma; CDHR13; CS2; CSTN2"
                     /inference="alignment:Splign:2.1.0"
     exon            2673..2857
                     /gene="CLSTN2"
                     /gene_synonym="ALC-GAMMA; alcagamma; CDHR13; CS2; CSTN2"
                     /inference="alignment:Splign:2.1.0"
     exon            2858..14202
                     /gene="CLSTN2"
                     /gene_synonym="ALC-GAMMA; alcagamma; CDHR13; CS2; CSTN2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      14172..14177
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLSTN2"
                     /gene_synonym="ALC-GAMMA; alcagamma; CDHR13; CS2; CSTN2"
                     /note="hexamer: AATAAA"
     polyA_site      14202
                     /gene="CLSTN2"
                     /gene_synonym="ALC-GAMMA; alcagamma; CDHR13; CS2; CSTN2"
                     /note="major polyA site"
ORIGIN      
        1 agtgagcgcg gctgctgccg gcgagctagc ggcgcagcgg cgggaacccg aggccgagcg
       61 ccgcggcggc agcgctagaa gcgcacccat cgggcacggc gaggcggccc acggtgcggc
      121 aggcaccggg aggcgagagc cggcgcggac agtaggcggc ggctgcagct cgttggcggc
      181 tgctgcgagg atgctgcctg ggcggctgtg ctgggtgccg ctcctgctgg cgctgggcgt
      241 ggggagcggc agcggcggtg gcggggacag ccggcagcgc cgcctcctcg cggctaaagt
      301 caataagcac aagccatgga tcgagacttc atatcatgga gtcataactg agaacaatga
      361 cacagtcatt ttggacccac cactggtagc cctggataaa gatgcaccgg ttccttttgc
      421 aggggaaatc tgtgcgttca agatccatgg ccaggagctg ccctttgagg ctgtggtgct
      481 caacaagaca tcaggagagg gccggctccg tgccaagagc cccattgact gtgagttgca
      541 gaaggagtac acattcatca tccaggccta tgactgtggt gctgggcccc acgagacagc
      601 ctggaaaaag tcacacaagg ccgtggtcca tatacaggtg aaggatgtca acgagtttgc
      661 tcccaccttc aaagagccag cctacaaggc tgttgtgacg gagggcaaga tctatgacag
      721 cattctgcag gtggaggcca ttgacgagga ctgctcccca cagtacagcc agatctgcaa
      781 ctatgaaatc gtcaccacag atgtgccttt tgccatcgac agaaatggca acatcaggaa
      841 cactgagaag ctgagctatg acaaacaaca ccagtatgag atcctggtga ccgcctacga
      901 ctgtggacag aagcccgctg ctcaggacac cctggtgcag gtggatgtga agccagtttg
      961 caagcctggc tggcaagact ggaccaagag gattgagtac cagcctggct ccgggagcat
     1021 gcccctgttc cccagcatcc acctggagac gtgcgatgga gccgtgtctt ccctccagat
     1081 cgtcacagag ctgcagacta attacattgg gaagggttgt gaccgggaga cctactctga
     1141 gaaatccctt caaaagttat gtggagcctc ctctggcatc attgacctct tgccatcccc
     1201 tagcgctgcc accaactgga ctgcaggact gctggtggac agcagtgaga tgatcttcaa
     1261 gtttgacggc aggcagggtg ccaaagtccc cgatgggatt gtgcccaaga acctgaccga
     1321 tcagttcacc atcaccatgt ggatgaaaca cggccccagc cctggtgtga gagccgagaa
     1381 ggaaaccatc ctctgcaact cagacaaaac cgaaatgaac cggcatcact atgccctgta
     1441 tgtgcacaac tgccgcctcg tctttctctt gcggaaggac ttcgaccagg ctgacacctt
     1501 tcgccccgcg gagttccact ggaagctgga tcagatttgt gacaaagagt ggcactacta
     1561 tgtcatcaat gtggagtttc ctgtggtaac cttatacatg gatggagcaa catatgaacc
     1621 atacctggtg accaacgact ggcccattca tccatctcac atagccatgc aactcacagt
     1681 cggcgcttgt tggcaaggag gagaagtcac caaaccacag tttgctcagt tctttcatgg
     1741 aagcctggcc agtctcacca tccgccctgg caaaatggaa agccagaagg tgatctcctg
     1801 cctgcaggcc tgcaaggaag ggctggacat taattccttg gaaagccttg gccaaggaat
     1861 aaagtatcac ttcaacccct cgcagtccat cctggtgatg gaaggtgacg acattgggaa
     1921 cattaaccgt gctctccaga aagtctccta catcaactcc aggcagttcc caacggcggg
     1981 tgtgcggcgc ctcaaagtat cctccaaagt ccagtgcttt ggggaagacg tatgcatcag
     2041 tatccctgag gtagatgcct atgtgatggt cctccaggcc atcgagcccc ggatcaccct
     2101 ccggggcaca gaccacttct ggagacctgc tgcccagttt gaaagtgcca ggggagtgac
     2161 cctcttccct gatatcaaga ttgtgagcac cttcgccaaa accgaagccc ccggggacgt
     2221 gaaaaccaca gaccccaaat cagaagtctt agaggaaatg cttcataact tagatttctg
     2281 tgacattttg gtgatcggag gggacttgga cccaaggcag gagtgcttgg agctcaacca
     2341 cagtgagctc caccaacgac acctggatgc cactaattct actgcaggct actccatcta
     2401 cggtgtgggc tccatgagcc gctatgagca ggtgctacat cacatccgct accgcaactg
     2461 gcgtccggct tcccttgagg cccggcgttt ccggattaag tgctcagaac tcaatgggcg
     2521 ctacactagc aatgagttca acttggaggt cagcatcctt catgaagacc aagtctcaga
     2581 taaggagcat gtcaatcatc tgattgtgca gcctcccttc ctccagtctg tccatcatcc
     2641 tgagtcccgg agtagcatcc agcacagttc agtggtccca agcattgcca cagtggtcat
     2701 catcatctcc gtgtgcatgc ttgtgtttgt cgtggccatg ggtgtgtacc gggtccggat
     2761 cgcccaccag cacttcatcc aggagactga ggctgccaag gaatctgaga tggactggga
     2821 cgattctgcg ctgactatca cagtcaaccc catggagaaa catgaaggac cagggcatgg
     2881 ggaagatgag actgagggag aagaggagga agaagccgag gaagaaatga gctccagcag
     2941 tggctctgac gacagcgaag aggaggagga ggaggaaggg atgggcagag gcagacatgg
     3001 gcagaatgga gccaggcaag cccagctgga gtgggatgac tccaccctcc cctactagtg
     3061 cccaggggtc tgctgcctgg cccacatgtc ccttttgtaa accctgaccc agtgtatgcc
     3121 catgtctatc atacctcacc tctgatgtct gtgacatgtc tgggaaggcc ttctccagct
     3181 tcctggagcc caccctttaa gccttgggca ctccctgtgt ttcatccatg gggaagttcc
     3241 aagaagccca gcatggccat cagtgaggac ttcagggtag actttgtcct gtagcctcca
     3301 cttctgccct aagttcccca gcatcctgac tacctgtctg cagagtttgc ctttgttttt
     3361 tcctgcaggg aagaaggccc acctttgtgt cactcacctc cccaggctca gagtccccaa
     3421 ggccctgggg ttccaactca ctgtgcgtct cctccacaca gaccagtagg ttctcctatg
     3481 ctgactccag gttgcttcat acaaggaggg tggttgaact tcacacacgt aaggtcttag
     3541 tgcttaacag tttaaaggaa agtccttgtt gaggcagaac taagtttaca gggaaaggta
     3601 cacacattct ctctctctct ctctctctgt ctatctagtt ccccagcttg gagagccttt
     3661 ccccttgctt ctttctgagg ccatataagc ttataagaaa agtcccaaac caagaatagg
     3721 tccttggcca caagcagggt ctgatccccc atcagagcta tctgagcctg cctgtctggg
     3781 cacctgctgc aaccatgcag ctaccctgcc aggggcactc agcaaacaga accacagggc
     3841 ccaggaggca ttccacacag gcactgcccc aggacaacac aacaaggaca gtcacaacaa
     3901 ggacaacaag gacacaacac aacacacaac aaggacagtc acaacaagcc tagagccaga
     3961 aagcagatgg aaatgctaat gaggtcaaac gtaggcttca tggtgggtgg agtgggggtg
     4021 gctgggctcc cccaggacag aggggaccct gaggttggca aggctctcac cactcagcct
     4081 tatggtccct tatctcctat cttccctctt gagaaaatac acgctttctg catgtattag
     4141 aaacgcacga gctccaccaa gtctacaatg aaagtttgaa atttaactgc aaggaattag
     4201 aagcatattt gcaatcattg cagcttcttc tttcttctgc tcataaaagg aggaacactt
     4261 tagatagagg gcaaatatat ctgaaaacct aatttctttc tttttttgat aaggaaatct
     4321 tttccatctc catcctaaca tgcacaacct gtgaagagaa ttgtttctat agtaactggt
     4381 ctgtgatctt ttgtggccaa gagaatagca ggcaagaatt agggccttga cagaatttcc
     4441 acgaagctct gagaacatgt ttgtttcgaa tgtctgattc ctctttgtca tcaatgtgta
     4501 tgctctgtcc ccatccttca ctcctcctca agctcacacc aattggtttg gcacaggcac
     4561 agagctggtc cctagttaag tggcatttat gttaaaaaaa aatagttcag aatctcagcc
     4621 ttttctttgt gtcatcaaaa cagcttaaga aggggactac tgccaatgtc ctctagtctg
     4681 acctccaccc agggaggacc catggcaggt cttttcaact ttctgattca tgagaacaac
     4741 cttgtgaagc ttttcccacc tcctaaagtg ttttctgcat ctgttccttc ctttggacct
     4801 cacaacaaat cctgtgaagt aactgagaca tctgttgtta gatacatttt tgtgatgagt
     4861 aaactgaggc ttcgtgattc aatgtcttgt tcagagtcat tcaaaagtat agacaagacc
     4921 agtctcccca acttccactc ctggtgcagg attctactta tgttcatagg tatgcaccta
     4981 acagacactg ctgcaaacct actatgtgct aagcatccta ccaatagctg tgagaaaaag
     5041 aaaagctgaa taagataagc tccttcccta cattatattt gcagacggga aataaacagg
     5101 cctagaaatt acacaagaga caaatgaaga ggaagagaag tcacaagaga agaaggatgc
     5161 ggtcacaaac ttaaccagtg ctcttatttt tcccttctca gggaaaaaca ccagaagaag
     5221 ctggggtttt atattcattg gatggaaaca gatatttctc ctgggaattc tcccaaccaa
     5281 atagcccttt tctattaggc tgtgtctgta catccacaag aggaaagtct ataggactga
     5341 ttagttttca caatcccatt taaataaaaa aaagatcttt agttgggcat ttaggagcaa
     5401 ctgaattctc ccaaaattgt ggcagcaaaa agaacacttc atatgcttaa aacccattct
     5461 ttttgaatta gattttgatt ttaaatattt aagtctggtg tttgtgagaa cacttttctc
     5521 tttgctcagt cttttcgatc tggttgggta gggaaggtgt tcctcatgct ctctgggtgg
     5581 ctgccttatt catagttgag agagctgtta ggtaaaatct ttatatgtgt tggggttttt
     5641 ttggtagggg gggtagcagg taaaggtggc ccatggctag ggcagcaaag atgaataatt
     5701 tgaggaagca tttggttggt ggactcattt gcagagaaac agtggtgaca acaaatcaga
     5761 gctgtttccc tcaactctgg ctataagagg tcattctctg catgtcagct acaaagatca
     5821 ggcacttgaa tgaatccgct gggttggcag gcattggtca tcttcgcaac accctcttcc
     5881 tcaccatgga tgagaacaag acgggagtgg ttatatccca gtaacagaag ctttgggagg
     5941 tatccaggtg agcagggtaa tgtggcttgc cctcccccag atattctgat cccaagaaaa
     6001 aggtgtcatt aatcagtctg gcattttcca ccctccatat gctggcatcc aggaaccaaa
     6061 acccctgaag gcctagacac cctatgacct ctaactgaga tgttggcagc caggcagatg
     6121 caggctcatg tccaggagga actgctgact ggtttggcca cagcccagtt cattgcttgt
     6181 cttgccaagt ggacaaccag attctgcctt cctgagcttg aaaataggct acaactctca
     6241 ggtgattcag atgacatgga ggagaagttg gaggagaaga ttgaaatgct taaatcccag
     6301 tgcagaatga gttgcttcag tggagtaaag caaccggcta gttggtagcc tgccttaaaa
     6361 aatactaaac atatatcatc atacatttga tcaaatgctg taagcacagc caaggcccaa
     6421 attcatctag ataccaaaaa tgtgatttca gtgaaatctc ttttcattgc cgctttcttt
     6481 tgttgttgtt tgttttttga gccagagtct tgctgctctg tcacccaggc tggagtgcag
     6541 tggcacaatc tcagctcact gcaacctcca cctcccgggt tcaagcgagt ttcctgcctc
     6601 agactcccga gtagctggga ttacaggcat ccaccaccat gcccagctaa tttttgtatt
     6661 tttagtagag acagggcttc accgtgttgg ccaggctggt ctcaaactcc tgatctcagg
     6721 tgatctgccc accttggcct ccaaggtaat gagattatag gagtgagcca ccgcacccag
     6781 ccctcattac cactttcttg aatttactca atcatcctat caagcagggt ttagaccaac
     6841 atctttgcat ttaataaggg ctctactttg tatacatgga ccatccagtg agagagctgg
     6901 gggtggtgag aagcaatgtc ccccattcct atatgtcagg tgtcagcaaa ctatctgtaa
     6961 agaggcaaat agtaaatatt ttgggctttg tgagccattt ggtacctgtc acagaatagt
     7021 cagccctcca tatctgaggt ttccaaatcc atggattcaa ccaaccttga atcaaaaata
     7081 tttgggaaaa aatgtatagt tgcaactgac ctgaacatat tctttttttc attattattc
     7141 cttgaacaat acagtttaac aggatttaca tagcatttac actgtattag gtattataag
     7201 taacctagag atgatttgaa gaacacagga agttgtgcat aggctatatg caaatactat
     7261 gcattttata taaaggaatt gaacatctgt ggattttggt gtccgagggg agttctggaa
     7321 ccaatcccct acagatactg agggatgact gtactcagct ctcctattgt agcatgaaaa
     7381 cagacaatac gtacaacaaa tgaatgtttc aatacaactt tatctagttt ccataaaaca
     7441 atttaaaaac actaaaaaag caggcagttt ggatttatgt aattttcata tcttttttta
     7501 cccttttgat ttacattttt aataatttaa aaatataaaa accttcctca gcccacaggc
     7561 catacgaaaa ttagcagcca gctagatttg gcccaaggcc aaagtttggc aacccgtacc
     7621 atacaccatg gaagttagcc ttccacctca ggagaaagca agctgctctc ataagcctct
     7681 caccaactac ccagtagtct ttgcctccaa actgaacaac aggaaaaagt gccatagttt
     7741 ttaattaagc tgttttaaaa gtccatgttc cgggggaaaa caaaaaagaa acaaacaaaa
     7801 cacttgttat ttgctgaact gacaaactga cagatattcc acgagagttg aaagccttgg
     7861 atcgaggtga ttgcctggtt actgagtgct aactccactg aggcaagtgt tagcccccca
     7921 aggctcagtg ccttcccagc acagctcagc ccataatgat ctctgagcag gagtgttcat
     7981 tatctcagac tgattttcat ttcagcccta ctcttgccat tgctctaaaa atgatcctaa
     8041 agacttgtga ctgtttgaat caatagcaac atcatcagta gaaagcccga ccaagcacac
     8101 ttcgaaatta gcaatcttct tggctctatg gctcaatatc agcaagaaga aaaaaagaca
     8161 actttttttt taatcatccc cacatttaaa ttaaccaaat agtttttaca aaaaagtagg
     8221 taagtgggtt taattctttg aaagtatttg ccagtgaaaa gtagatgatt tttaggtaag
     8281 ataacgttta ggcctctggg ggatccccaa agccttctgc aatgtactct ggcaggctat
     8341 gaatggtaca ggattctgca tctatatgag acatttattg aggctgacaa gcaaaagccc
     8401 ttggcacaat ttaattttca cataaccact ttccattgtc cagctgcagg caaaagaaat
     8461 gagcaatgaa agttttgaag tctaccccag taccctcatt tcctgtcttt atttccttta
     8521 ctttatagat gtattctata cctgctatgt agataatgct ctagttttcc ctttagtgcc
     8581 aactggaaaa ggagagttgg ctttacaggg gctgtggatt aagagccagg cttcattcca
     8641 tcccagggag acaaaaagag acagaagaag ttacctccag cagcacccca gaaagctgac
     8701 agatattccc tgtatgcagg tagtaaacag atgggagtct gtgtttgcag ctatgttcag
     8761 tgtaattcag cagaccttca ctgggcactt gtgatgggtc aggccctgtg ccagcaacag
     8821 gggaagggag gatgaacaag gcatgtttcc ctgaccatga ggagctcttg gcctgtgagc
     8881 tgttgggatg tccctttgtg gtgttggaag ctgtgcacga gcagtcagga ctttggggac
     8941 agaagtttag tgttagggag gcagggtaac ttgctttgac agctacccac cctaagtcag
     9001 atgacaggtg taattctagg ggccctgata tgcatcactg aggcagactg aggagctctc
     9061 cagggaagta ggcaatattt ccctgaatat tcctcaattc caatcaaggc tgactcagtt
     9121 cacttatgga tgaaagccag ttaccctgcc cactgggcag aaataacatg gttgacagct
     9181 gacattcaga gaaaataaga cagaagcagt ccttaaatat aaacagcagc caggtggacc
     9241 tggaaattta ggaagggtga tgcaggtaga ggggccagca ggagccaagt ctcaaaaggc
     9301 acaagccaga ctgatgcaag ggagccctcc tgaggcccaa catcagagct gaagatctaa
     9361 ggagggccca cctccacgag acctgctgag ggcttacatt tggtgctgag gagcagtggt
     9421 tccctgggcc agctgcatca cctgggaaca tgtgagaagt gcatattctt gggccccacc
     9481 ccagacctac tgaatgagaa gctctggagg caggcagtct gtgatttaat gagctttctg
     9541 acacacacta aagtttgaga accactgatg cagaggatga ggagatgact tgagctggca
     9601 tcgggaagaa ctgactatta actaacaagg taaaatcaat gactagtgca gtgagggtgc
     9661 caaagcaaac cacccccagc ccccatcctg ggcctttttg gctccagttt atactcactg
     9721 aagcagtggt ttcaaggaac cttggtccta gtccgcatcc ttcacctgcc cttgggcatg
     9781 agacaattcc tgcctgcctg ccccttctat cctagagaga gagactgccc aggagaggct
     9841 gccatgaggt cactcatccc caggaaggcc acctgccagg tttctgtaag ctgagcagcc
     9901 tcttcgaggg ccatattttg tggagaaaca ctgaaactgc agcagtgacc ctggtcacta
     9961 ggagagctgc tttctacaca gcctgtatgc tggtggagac acccagggcc tggcagagtg
    10021 tggagggggt catttaagga cgttgccagg ttgccgcagc actgctctgt ttgtttgtaa
    10081 tgagactggg gcatgcagca tcacaattca aacactgaat tgcttgggga atggatggcc
    10141 ttccttggaa gggcagtaac tcattctgga tgttactaaa agtagaaaac aaaaatccaa
    10201 aaacaaacct acagcctcat ttacaattag aatctgtctg ggccttctga tttctaaagt
    10261 ctgtgaggct gccaggattc accaattctc ctttcccctg aggcccagca actattgatt
    10321 tatagcttaa actctgtgaa ggtccacaga cctttgaaag gaacttgctg gtactttgtc
    10381 agtcgtgtac acctaactga ctcctcctac gaggtacagt ctcctcatac aaggggtttt
    10441 gagatggaga gtcagagggc cagggccctg ggctttgggg tggaaggaca gaagagtagc
    10501 agtctaggaa agggactaga catgtagcct aggaaaacta gcaccatctg ttgagttctg
    10561 aagaacccag attctctcct gctcacagcc tcagcagtct tttcagagtc ctcctgaatg
    10621 gcccaaagac acccctgttt ctggggaaca gaagggagct ggtacctctg gaccattgag
    10681 gcaacacctc cccagaggca ggaccccgcc cccatgagaa catgcagttg aatgatgtgg
    10741 tgcctggcca ggaccttgga aaacgtggcc atgtgagact agtatagatc tccaactatt
    10801 gctgcctctg ctgatatggc cacctcctta aggtaagaag actaccgact tagctattgt
    10861 ggcaccacgg gagcctagca ctgcacctgg cccatgacat gtgtgccaca aatgttgaat
    10921 tgaattgaat cccagagatc acttgacctc agtgaaggga ctgggtttga agaattaaga
    10981 ccccccagtg gagaaagcct cctccaactt ccttctcggt ttgtgagtca aggtcctttg
    11041 taaaatcaga tcgacgaggg tggaagtgca gtgaacagag gacaacattt gtgatgcacg
    11101 tgggagaaaa caggagtggg gttgtttggg gactacccct tcctagctat agtcagtaaa
    11161 tggagcccct aagcaaggag caggggtaga tgtgcctggg tgaggcctgc ttgctccacc
    11221 gtcgaggtca tgggcagcat cccaactgag gagagaaaac cctaatttgt taatgaccca
    11281 gccttgtgct gctctccagt tcctttcatc agatttactg cagagcagat tcatcaacat
    11341 gaagttcaat tcccagtaga ctaatgaaaa gagggcaaca gacttgagaa gagccaaaat
    11401 gcagacagtg tgtattaatc acagacaaca gaaacgtgtt agctttaatg tctgtttgct
    11461 tttatcttga aatgaaaatg catctctgtt acctggaccc tttttactgc acatttcaga
    11521 acagaaatgg gacacccaca cacgtattga gggtcctttt ccccggcagg aggagctacc
    11581 agagaaatga gcccacattt tgtgtgcaga gagaatcaac ccacaggtct cagctgcaga
    11641 ctccagcacc tctgcagaga aggcaggcaa agggatgagg aggtagcatc tcctcttcag
    11701 aaggattcat aagggcctct gtgaggaggg ccttgcttgt gcctgtgggt catggatgtt
    11761 aatataaagg ctcatccacc cttggtggca aaagtgcatc catcctggcc aatatagaac
    11821 actcacatct ggagcccacg cccacccacc tccagctcct ctacatggct gcaagagcat
    11881 ggctgatgtg tgggattcag aagaggtctg ctcatgttca ctagccaaac tttaggtggt
    11941 gtgatacagc aaaaagcttt ttttgttttt ctgtttttgt tttgtttttg gcgtggagga
    12001 gaaatttaga cttgaaagtc agggctgatc actcaaaaag taagatagga gatattgagg
    12061 gaaaatgtga ctcatagttg gagaggaaac aggagagtgg gtacaagtga cagcaccagg
    12121 aacaaccccc tcccccagat gccaaagcat tgctctgtat ctaaagatgc atccaagttt
    12181 gcaaaaaatt atgggtaaat ctggccatgt gaccaaagag gcagtcaaat caattgcttc
    12241 caaagtcaga gctgtttgaa ggtgatcagc tcttaattga actagtcatg atgaatcaat
    12301 ccaactgact gcatccagtc tgacctcggt ggtttccata aagggttgtt gatgtcctga
    12361 gctggacagg gtgggcagga ggttgaagaa aaccagttgg tgagtataga aaagaaattg
    12421 catgtagaag ttgtttgttc tcaaagaaat ctgtaggtgt tagagctggg tttgttatgt
    12481 tattgtcagg gacattataa tattggtaag gaggagagaa tgatttggta ggggtattcg
    12541 agaaatttga gtacaaaata taaattatca gagatgagta ggctatggac agctgattat
    12601 atatatgtgt gtgtgtgtat atgtgtgcat atatgtgtac atatacacat accccaaagc
    12661 catatacata tctataatac agaagtaaga ataaagtcat aagtaccagc atattttaaa
    12721 tccttagaag cactggttgg gatgttacat gttttttcac tgttgttgtt taccgttagc
    12781 tctcaaagca gtgggaatag tgcttggcac atagtaggag tttggtaaac atttggaaat
    12841 tgaagaccca gcttatcact ccttcataga gactcagtag tatgagtata ccccaaattg
    12901 aatgagccac agagaaatgc atccagccac actccctctt agctgaggag aagcctctgt
    12961 gaatccacag attccatgca atattcagcc agccgcagac acaaggtctg gctcccttgt
    13021 cgtgagagtg aaggcatgct gcacacagtg ggtttgcatg cacctagact gcatctgctg
    13081 aacacccaac agctgtttct acaagctaaa gttgtagctt ctctctacca ctgtctaaag
    13141 ctgtagcttg tctctactat tgtctctaca ccgttgccca aacacttggt ataatgctat
    13201 tgaatttttc acctccaggc atgattttgt aaccaatgta aaggttctgg gttcagccag
    13261 tctaaaaagt ctaataattt cttctctcag tccttccctt ctcccttgtc tggagctgcc
    13321 accgccattt aaaagtgttg gacgctgttt attcccagct tctcaacctc agtgtgtgtg
    13381 ggaaaggcct gaaagagaga ggtatgtttt ctctcctcca gagtctctgt ggtgttgaca
    13441 cagcagagag aggaggggga gcagcctcgt cttgcagaat caccttcaat cactgctact
    13501 gtctgggcca ttactgccag tctggccggt gtctcccaca gtggatggct ttgcctccag
    13561 cggtggacca gtgtgtacct gacatgtatc agacttctga gctcttcaag ctgcctcaaa
    13621 gaatgaatct ttttagtcta gaaaatgtgt ttatttgata aatatactat tgtgtatgag
    13681 tgtgaaacaa tgcagacttt ggagtatctc attagaagga ctgtatgaat ttatagaaaa
    13741 ttgaatctaa tttcagaaga gcgcactgtc ttctcagtca acaaggttgc ccagccacag
    13801 agggtcagag aaaatgtcct ttcccctccc cactcctttt catagaatcc tctctcaggc
    13861 ctaactgagc tgtcatatcc aattcagact gacacagagt gaggggcgct gagagtctct
    13921 atgtatataa agatatagga agaaaataag gatcatcaca ggaatctgtt gggcttctcc
    13981 ccatgactgt gatcttaccc atgtttgaat cacaaacttt aaatttataa taattatgat
    14041 tcttgcagtt ttcaagtaat ttgttaaatg cctattcaac acagacagat ttttattttc
    14101 agctgtggct tgtaaatgcc tacaaactga ttcatgttgg tggtcgttat gacactttct
    14161 gtgaattggt gaataaatat gattttagaa tttcacagta aa
//
