LOCUS       NM_018941               7084 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens CLN8 transmembrane ER and ERGIC protein (CLN8), mRNA.
ACCESSION   NM_018941
VERSION     NM_018941.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Badura-Stronka,M., Winczewska-Wiktor,A., Pietrzak,A.,
            Hirschfeld,A.S., Zemojtel,T., Wolynska,K., Bednarek-Rajewska,K.,
            Seget-Dubaniewicz,M., Matheisel,A., Latos-Bielenska,A. and
            Steinborn,B.
  TITLE     CLN8 Mutations Presenting with a Phenotypic Continuum of Neuronal
            Ceroid Lipofuscinosis-Literature Review and Case Report
  JOURNAL   Genes (Basel) 12 (7), 956 (2021)
   PUBMED   34201538
  REMARK    GeneRIF: CLN8 Mutations Presenting with a Phenotypic Continuum of
            Neuronal Ceroid Lipofuscinosis-Literature Review and Case Report.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7084)
  AUTHORS   Meng,N., Wang,X., Shi,Y., Mao,Y., Yang,Q., Ju,B., Zhu,Q., Zhang,T.,
            Gu,Y. and Zhang,X.
  TITLE     miR-3074-5p/CLN8 pathway regulates decidualization in recurrent
            miscarriage
  JOURNAL   Reproduction 162 (1), 33-45 (2021)
   PUBMED   34044364
  REMARK    GeneRIF: miR-3074-5p/CLN8 pathway regulates decidualization in
            recurrent miscarriage.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7084)
  AUTHORS   Bajaj,L., Sharma,J., di Ronza,A., Zhang,P., Eblimit,A., Pal,R.,
            Roman,D., Collette,J.R., Booth,C., Chang,K.T., Sifers,R.N.,
            Jung,S.Y., Weimer,J.M., Chen,R., Schekman,R.W. and Sardiello,M.
  TITLE     A CLN6-CLN8 complex recruits lysosomal enzymes at the ER for Golgi
            transfer
  JOURNAL   J Clin Invest 130 (8), 4118-4132 (2020)
   PUBMED   32597833
  REMARK    GeneRIF: CLN8 associates with CLN6 to form the EGRESS complex
            (ER-to-Golgi Relaying of Enzymes of the lySosomal System), the
            functional unit responsible for the recruitment of newly
            synthesized lysosomal enzymes in the endoplasmic reticulum and
            their transfer to the Golgi complex.
REFERENCE   4  (bases 1 to 7084)
  AUTHORS   Parvin,S., Rezazadeh,M., Hosseinzadeh,H., Moradi,M., Shiva,S. and
            Gharesouran,J.
  TITLE     The Neuronal Ceroid Lipofuscinoses-Linked Loss of Function CLN5 and
            CLN8 Variants Disrupt Normal Lysosomal Function
  JOURNAL   Neuromolecular Med 21 (2), 160-169 (2019)
   PUBMED   30919163
  REMARK    GeneRIF: The Neuronal Ceroid Lipofuscinoses-Linked Loss of Function
            CLN5 and CLN8 Variants Disrupt Normal Lysosomal Function.
REFERENCE   5  (bases 1 to 7084)
  AUTHORS   Lonka,L., Kyttala,A., Ranta,S., Jalanko,A. and Lehesjoki,A.E.
  TITLE     The neuronal ceroid lipofuscinosis CLN8 membrane protein is a
            resident of the endoplasmic reticulum
  JOURNAL   Hum Mol Genet 9 (11), 1691-1697 (2000)
   PUBMED   10861296
REFERENCE   6  (bases 1 to 7084)
  AUTHORS   Dawson,G. and Cho,S.
  TITLE     Batten's disease: clues to neuronal protein catabolism in lysosomes
  JOURNAL   J Neurosci Res 60 (2), 133-140 (2000)
   PUBMED   10740217
  REMARK    Review article
REFERENCE   7  (bases 1 to 7084)
  AUTHORS   Ranta,S., Zhang,Y., Ross,B., Lonka,L., Takkunen,E., Messer,A.,
            Sharp,J., Wheeler,R., Kusumi,K., Mole,S., Liu,W., Soares,M.B.,
            Bonaldo,M.F., Hirvasniemi,A., de la Chapelle,A., Gilliam,T.C. and
            Lehesjoki,A.E.
  TITLE     The neuronal ceroid lipofuscinoses in human EPMR and mnd mutant
            mice are associated with mutations in CLN8
  JOURNAL   Nat Genet 23 (2), 233-236 (1999)
   PUBMED   10508524
REFERENCE   8  (bases 1 to 7084)
  AUTHORS   Ranta,S., Lehesjoki,A.E., Hirvasniemi,A., Weissenbach,J., Ross,B.,
            Leal,S.M., de la Chapelle,A. and Gilliam,T.C.
  TITLE     Genetic and physical mapping of the progressive epilepsy with
            mental retardation (EPMR) locus on chromosome 8p
  JOURNAL   Genome Res 6 (5), 351-360 (1996)
   PUBMED   8743986
REFERENCE   9  (bases 1 to 7084)
  AUTHORS   Mole,S.E. and Williams,R.E.
  TITLE     Neuronal Ceroid-Lipofuscinoses - RETIRED CHAPTER, FOR HISTORICAL
            REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301601
REFERENCE   10 (bases 1 to 7084)
  AUTHORS   Klein,C., Lohmann,K., Marras,C. and Munchau,A.
  TITLE     Hereditary Dystonia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301334
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA193710.1, AF123761.1,
            BC007725.2, AC100810.18 and AW296697.1.
            
            On Aug 31, 2019 this sequence version replaced NM_018941.3.
            
            Summary: This gene encodes a transmembrane protein belonging to a
            family of proteins containing TLC domains, which are postulated to
            function in lipid synthesis, transport, or sensing. The protein
            localizes to the endoplasmic reticulum (ER), and may recycle
            between the ER and ER-Golgi intermediate compartment. Mutations in
            this gene are associated with a disorder characterized by
            progressive epilepsy with cognitive disabilities (EPMR), which is a
            subtype of neuronal ceroid lipofuscinoses (NCL). Patients with
            mutations in this gene have altered levels of sphingolipid and
            phospholipids in the brain. [provided by RefSeq, Jul 2017].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF123761.1, SRR14038192.1799885.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000331222.6/ ENSP00000328182.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-53                DA193710.1         86-138
            54-61               AF123761.1         68-75
            62-1564             BC007725.2         1-1503
            1565-6800           AC100810.18        30339-35574         c
            6801-7084           AW296697.1         17-300              c
FEATURES             Location/Qualifiers
     source          1..7084
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p23.3"
     gene            1..7084
                     /gene="CLN8"
                     /gene_synonym="C8orf61; EPMR; TLCD6"
                     /note="CLN8 transmembrane ER and ERGIC protein"
                     /db_xref="GeneID:2055"
                     /db_xref="HGNC:HGNC:2079"
                     /db_xref="MIM:607837"
     exon            1..97
                     /gene="CLN8"
                     /gene_synonym="C8orf61; EPMR; TLCD6"
                     /inference="alignment:Splign:2.1.0"
     exon            98..763
                     /gene="CLN8"
                     /gene_synonym="C8orf61; EPMR; TLCD6"
                     /inference="alignment:Splign:2.1.0"
     CDS             221..1081
                     /gene="CLN8"
                     /gene_synonym="C8orf61; EPMR; TLCD6"
                     /note="ceroid-lipofuscinosis, neuronal 8"
                     /codon_start=1
                     /product="protein CLN8"
                     /protein_id="NP_061764.2"
                     /db_xref="CCDS:CCDS5956.1"
                     /db_xref="GeneID:2055"
                     /db_xref="HGNC:HGNC:2079"
                     /db_xref="MIM:607837"
                     /translation="MNPASDGGTSESIFDLDYASWGIRSTLMVAGFVFYLGVFVVCHQ
                     LSSSLNATYRSLVAREKVFWDLAATRAVFGVQSTAAGLWALLGDPVLHADKARGQQNW
                     CWFHITTATGFFCFENVAVHLSNLIFRTFDLFLVIHHLFAFLGFLGCLVNLQAGHYLA
                     MTTLLLEMSTPFTCVSWMLLKAGWSESLFWKLNQWLMIHMFHCRMVLTYHMWWVCFWH
                     WDGLVSSLYLPHLTLFLVGLALLTLIINPYWTHKKTQQLLNPVDWNFAQPEAKSRPEG
                     NGQLLRKKRP"
     exon            764..7084
                     /gene="CLN8"
                     /gene_synonym="C8orf61; EPMR; TLCD6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1257..1262
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLN8"
                     /gene_synonym="C8orf61; EPMR; TLCD6"
                     /note="hexamer: AAGAAA"
     polyA_site      1281
                     /gene="CLN8"
                     /gene_synonym="C8orf61; EPMR; TLCD6"
                     /note="major polyA site"
     regulatory      3237..3242
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLN8"
                     /gene_synonym="C8orf61; EPMR; TLCD6"
                     /note="hexamer: AATAAA"
     polyA_site      3256
                     /gene="CLN8"
                     /gene_synonym="C8orf61; EPMR; TLCD6"
                     /note="major polyA site"
     regulatory      7062..7067
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLN8"
                     /gene_synonym="C8orf61; EPMR; TLCD6"
                     /note="hexamer: AATATA"
     polyA_site      7084
                     /gene="CLN8"
                     /gene_synonym="C8orf61; EPMR; TLCD6"
                     /note="major polyA site"
ORIGIN      
        1 agtcgtgact gggcggcaat gaggtcagtg actgccggga gtcctgcagg ggcggggcgg
       61 cgccaagcgc agggagcccg gctgagtggc agcccagatt gaagatggat acgtgacaat
      121 cccagggacc gctgcactga cttcatttcc ttagacaaga cacagtgtag ggcccggccc
      181 gtgttggccc caggactcct ttggaatata gctgtggaca atgaatcctg cgagcgatgg
      241 gggcacatca gagagcattt ttgacctgga ctatgcatcc tgggggatcc gctccacgct
      301 gatggtcgct ggctttgtct tctacttggg cgtctttgtg gtctgccacc agctgtcctc
      361 ttccctgaat gccacttacc gttctttggt ggccagagag aaggtcttct gggacctggc
      421 ggccacgcgt gcagtctttg gtgttcagag cacagccgca ggcctgtggg ctctgctggg
      481 ggaccctgtg ctgcatgccg acaaggcgcg tggccagcag aactggtgct ggtttcacat
      541 cacgacagca acgggattct tttgctttga aaatgttgca gtccacctgt ccaacttgat
      601 cttccggaca tttgacttgt ttctggttat ccaccatctc tttgcctttc ttgggtttct
      661 tggctgcttg gtcaatctcc aagctggcca ctatctagct atgaccacgt tgctcctgga
      721 gatgagcacg ccctttacct gcgtttcctg gatgctctta aaggcgggct ggtccgagtc
      781 tctgttttgg aagctcaacc agtggctgat gattcacatg tttcactgcc gcatggttct
      841 aacctaccac atgtggtggg tgtgtttctg gcactgggac ggcctggtca gcagcctgta
      901 tctgcctcat ttgacactgt tccttgtcgg actggctctg cttacgctaa tcattaatcc
      961 atattggacc cataagaaga ctcagcagct tctcaatccg gtggactgga acttcgcaca
     1021 gccagaagcc aagagcaggc cagaaggcaa cgggcagctg ctgcggaaga agaggccata
     1081 gctgctccag ccggggctcc ggggcggcag cagagctggc acaccgattc tgggaagccc
     1141 cgcgaatgat ggcttttgaa ttaatgaggc agtgaatgtt ttgtgtttac ttctaaggga
     1201 aatactaact ttctttcgca ttagtattaa ttttgaagta gctacaaagt atttttaaga
     1261 aattataatt ttatgactgt ctggcaggct ctgtcagttt agccgcgccg gaccgtgtca
     1321 agcatctagg agaggagtcc atggtgtcca ggcatcgggg cgtcacacct gttgaggagt
     1381 ggggtggctt tgaatgctgg aaatggcttc atagtgaagt gcctcccaca gggcgggtgg
     1441 gtcagcgttg actctttcca gctgcacact catatgccgt gtgtcttatt cagaagtcac
     1501 attcttttca gttggagaga attgggctaa gatagaaaat aacatgattt gttccttatt
     1561 aaagcttccc agcgtatgaa attctaagct gggtggggtg gctcacaccc gacgtaatcc
     1621 cagcacgttg ggaggccgag gcaggtggat cacttgaggc caggagttcg agaccagcct
     1681 ggtcaagatg gtgaaacccc atctctacta aaattacaaa aattagccgg gtgtcgtggc
     1741 acacacctgt aatcccagct atttgggagg ccaaggcagg agaattgcct gaacccggga
     1801 ggcggaggtt gcagtgagct gagatcgcac cactgcactc cagcactcca gcctgggtga
     1861 cagagcaaga ctctctctca aaaaaaaaaa aaaaaaaaaa ttctagaccg aagtgtacgg
     1921 cagtgccatc tggtggcaag tggtacaaag acaacgctga ggggtagtga ctcctgtagc
     1981 agcagagacg ccttggagtt taacccccac caaccagagc gtgggctggt aaagatgaaa
     2041 tcttgcaagt tttttttttt tttaaatcat ggtacctgtt ttaaaatgag aagttattat
     2101 tcatactgtg ttgctcattt gacaaaataa ggtaaggatt tcataatcag gttgtctggg
     2161 tttcagagct gtttttaatt gactgagtta cctacaggca cccaatctag accctaattc
     2221 tgtggttggt gttcctctgt agatttcaag acaagtagtg atgtcatttt ctccctgagc
     2281 tgtagggtgg acctggaccc ctcagagatg ctcgactgca gggtcctggg gtccctagag
     2341 tggtcctgca gcagcccctg gcaatgagct gcctgatgat gagtcccaag gtacattgtt
     2401 ctactgtttt atacccaggt agaagttaca cagcaattta gcaatgttgt taacagacat
     2461 acagaattgt gtgtgtgtgt gtgtgtgtgt gtgtgagtgt tttgatataa acgtgcaatt
     2521 aaaattcaaa gtttattttt tatcacaagc acctttgaaa tgtacgtgga aaacgtcggc
     2581 tgctcgtgag aatcatgtgt gacttacgtt ccttatgttt tatggaactc tcggaacagt
     2641 gttttgtttt tgtttagttt ttgttttcgt ttttttgaga cggagcctgg ctttggctcc
     2701 caggctggag tccagtggtg tgatctcggc tcactgcaac ctccacctcc cgggttccag
     2761 ggattctcct gcctcaccct ttcaagtagc tgggattaca ggtacctcta ccatgcctgg
     2821 ctgatttttt gtatttttag tagagacagg gtttcaccat gttggccagg ctggtctctg
     2881 actcctgact aaaagtgatc tgcccacctc ggcctcccaa agttctggga ttacgggcgt
     2941 gagccacttc acccggcccg gagcggtgta ttttgattgc tggtgcttta actatattga
     3001 ccaaggtgta tagtaaccta tttatcacat tttacgaagc aattttaatc ttaaacacat
     3061 ccagcaaatg gtgtccctgc tgaaactttt aactggaaac ctcacacctg gcaggcagca
     3121 ctctctgcag ccgtgccctt gtggagtcag caggagctgg tgtttttcag ccagacagag
     3181 gcataataaa gtgactgatg tttacagaac ccaaatgatg tcttcctctc tctcaaaata
     3241 aactaaccta ttcatattgc tgtgaatgct ttatcttgga gatagaaaat ataatgataa
     3301 attcatcatg tgttcagaat tcgatttgga gttggaagtg gacagcctaa tttttatctc
     3361 acctccacta ggactttgac cttcccaggg gtccacactt tacctcctgt atcttcatac
     3421 gcgtgtgtgg atgcatagag atggaggtgc tgtagagaag cacaggctca cccgctccgt
     3481 ccagtgtcac ccacacattg gtccggggac ccctcagtgc agagcctgaa gttgggggtt
     3541 taggggtgga aaaggcttcg tgaaatgtta atgatgcgta caccttccag atggcacagc
     3601 actttcccat ggtcctcact ggcctcagtc tccctgaccg gcagtgtgag gggatgtagc
     3661 tatagtggag catggtacaa gcgacattca tgagctctga cttcttggtg ccaggttggt
     3721 gtgagcctgg gcccggccca ctcagcggcc cacattggcc agcattcagc tgcacgtgtg
     3781 tgctgcgtgc ttcacatcct ctattgagag ttacagcaag tgttaaacga ggtgagttca
     3841 cataacagga attctggaac tgcttgaaaa ctaggacgat tgggcaatat cggccttaac
     3901 tccacctgat ggcaggtgac ccggatagaa aatggccctg cgtttagcca ggatgtggct
     3961 ctccagcttg gcttcagtgt gatcacttgt cagtgcgctt tctctttcga tagtgaaatc
     4021 cttctctata cctatgtttt gttttgtttc ttaagttggg aaacagaatg ggccagggag
     4081 gttgagtgac tgaagaccaa gggttggtgc agcctcctcg ccgcgctgcg ggggctgggc
     4141 cgcacaggct tctgcccttc tcggtgtcca ggctccttgg gtgatgctgg agttgtcatg
     4201 gccgcagttc agtgtgagat tttttaccag gtattgcgct taagggacat gattttccat
     4261 tttcttcgcc cggacaactt gaatgaaatg ggcactgttg attccacttc tgtcgaggag
     4321 cttcggggct cagagaggtg atgacgtgcc caaggtgacg caactcgtga acagccgtgc
     4381 ctgccttggg cgcagcctcc ggcgccagag ctgggctctt caacacggca tttagcgcag
     4441 aaagtcgtgg ttcaggcagt atgggccgct gtgacaaaac acctaagact gggtagttta
     4501 taaagaacag acattcaggc caggcacggt gactcacgcc tgtaatccca gcactttggg
     4561 aggccgaggc gggtggatca tttgaggtca ggagtttgaa accagcctgg ccaacatggt
     4621 gaaaccccat ctctactaaa aaaacaaaac tagctggggg tggtggtgca tgcctgtggt
     4681 cccagctact tgggaggcta aggtagaaga attgcttgaa cctgggaggc agagattgca
     4741 gtgagccgag atcacgccat tgcactccag cctgggtgac acagtgagac tccatctcaa
     4801 aaaaaaaaaa agaacggaca tcatttctca cagttctgga ggctggaagt ccaagataaa
     4861 ggtgtgggct gattagtttc tgctgggaac actgcatcct cacgtggtgg aggggatgga
     4921 aggtcaacag gcctcagcta gttctccgct gcctttttat gaggcattaa gccatttgca
     4981 agagtagggg gccccacctc ttaataccac cgcaatgaca attacaatgt catgtgaatt
     5041 ctggagggga cacggtcaca ctgcagcatc agcacccggt gaatgtggtt ctcttcccat
     5101 gtccgaattt tgtgagctcc acacgggcag ggttgcatct gttttattct ctgattgatt
     5161 ctaagcccca tgtccctggc acaggatggc tgcttggcag tagggagtgg atgaaccagt
     5221 agtacccagg gctgccaggc agggccctgc aggggacaga cccaagggct gatatgggga
     5281 cacctgctga ggggggaatt taccacggta agggcagggc tgggaggctg ggcagccagt
     5341 ttcaccctcg cacaggctgc ctggcaagac cagcggctgg aggtgatgtc ctcagtagag
     5401 aacggtggcc actggaaacg tgtggccaga aggggaaacg ctcgggcctc tggtgggggc
     5461 cagcctgggg tggggtcggc cagggtcagc tgcctttcct tcatgctggg gaggagatgt
     5521 tgtgtgcatg tgagaaagca tttgatagca tgccaaaagt ttcctccatt atgtgcgtgt
     5581 caagtgtttg accaggtcag aactcttgtt ctatgcgaat gtgctcacgc agacagaagc
     5641 ggaggggctc cgtcgctctg ccctcacgcc gctgaaccgc gagggtctcc cgctcctcag
     5701 gcttgcggct cggccgcgaa gtgtgtcagg tgtgcatcct gggtgctcct ttgtgctctg
     5761 tcctacggtg acacaggcgg cgtggggtta gacaggtacc ggtcagacta cggtgacaca
     5821 ggcggcgtgc ggttagacag gtaccggtca gattacggtg gcacaggcgg cgtggggtta
     5881 gacaggtacc ggtcagatta cggtggcaca ggcggcgtgg ggttagacag gtaccggtca
     5941 gattacggtg gcacaggcgg cgtggggtta gacaggtacc ggtcagatta cggtggcaca
     6001 ggcggcgtgg ggttagacag gtaccggtca gattacggtg gcacaggcag cgtggggtta
     6061 gacaggtacc ggtcagatta cggtggcaca ggcggcgtgg ggttagacag gtaccggtca
     6121 gattacggtg gcacaggcgg cgtggggtta gacaggtacc ggtcagatta cggtgacaca
     6181 ggcggcgtgg ggttagacag gtaccggtca gattacggtg gcacaggctg cgtggggtta
     6241 gacaggtacc ggtcagatta cggtggcaca ggcggcgtgg ggttagacag gtactggtca
     6301 gatgcacggg ctccctaaac ccctgctgtg gcttcggcag taaagacagg acgcacccat
     6361 gtcacaagag gagcacaggc aggggtgttg gtgttggggc agccctcagg gtctccagac
     6421 cccagcccca ctcacacagc agcctaggaa ggaagggcag agtcccaggt gtcagctggt
     6481 gggtctccca ggagctgccc ctccctggaa gtcacaggac aggaatgaca gatcagggaa
     6541 ctgcaggaag ctgccacctc tggggtcaga atatgcccag cctgcggggg ctctctatcg
     6601 gggtcttcga gagccagaca gcctgccttg tgctgcatac ctggctttgc tctgtgcaga
     6661 acccagcaca cgtgattttg tgtgacatgc cagcagcctg gctcccagga caggaggcct
     6721 gccctggggg aggggctgca ggaggagggg gggcaggcac ccatgagtct gtccagcctt
     6781 gtcacagatg catcgcccac cctgcggtcc tgatttcagc tcacctcaga gtaaatcaga
     6841 ataaactgca cccagacttt cacgaatgca tgttgacgct ttcagttcac ccctttcttt
     6901 gctaactttc ttcctatttt cttctaatgc gagagcttat taattccata tttatcattt
     6961 tgaataactt ttctcctttt tagtaacaaa atgtacttca ctcttagtaa aatgtattta
     7021 ctattttagt aacaaaaata tacttgccta atcatgttta aaatatagtg atgtgaaaaa
     7081 ttca
//
