LOCUS       NM_020666               2504 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens CDC like kinase 4 (CLK4), mRNA.
ACCESSION   NM_020666
VERSION     NM_020666.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Petsalaki,E. and Zachos,G.
  TITLE     Clks 1, 2 and 4 prevent chromatin breakage by regulating the Aurora
            B-dependent abscission checkpoint
  JOURNAL   Nat Commun 7, 11451 (2016)
   PUBMED   27126587
  REMARK    GeneRIF: Clk1, Clk2 and Clk4 prevent chromatin breakage by
            regulating the Aurora B-dependent abscission checkpoint.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2504)
  AUTHORS   Eisenreich,A., Bogdanov,V.Y., Zakrzewicz,A., Pries,A., Antoniak,S.,
            Poller,W., Schultheiss,H.P. and Rauch,U.
  TITLE     Cdc2-like kinases and DNA topoisomerase I regulate alternative
            splicing of tissue factor in human endothelial cells
  JOURNAL   Circ Res 104 (5), 589-599 (2009)
   PUBMED   19168442
  REMARK    GeneRIF: Cdc2-like kinases and DNA topoisomerase I regulate
            alternative splicing of tissue factor in human endothelial cells.
REFERENCE   4  (bases 1 to 2504)
  AUTHORS   Wang,A.G., Yoon,S.Y., Oh,J.H., Jeon,Y.J., Kim,M., Kim,J.M.,
            Byun,S.S., Yang,J.O., Kim,J.H., Kim,D.G., Yeom,Y.I., Yoo,H.S.,
            Kim,Y.S. and Kim,N.S.
  TITLE     Identification of intrahepatic cholangiocarcinoma related genes by
            comparison with normal liver tissues using expressed sequence tags
  JOURNAL   Biochem Biophys Res Commun 345 (3), 1022-1032 (2006)
   PUBMED   16712791
REFERENCE   5  (bases 1 to 2504)
  AUTHORS   McNally,T., Huang,Q., Janis,R.S., Liu,Z., Olejniczak,E.T. and
            Reilly,R.M.
  TITLE     Structural analysis of UBL5, a novel ubiquitin-like modifier
  JOURNAL   Protein Sci 12 (7), 1562-1566 (2003)
   PUBMED   12824502
REFERENCE   6  (bases 1 to 2504)
  AUTHORS   Kantham,L., Kerr-Bayles,L., Godde,N., Quick,M., Webb,R.,
            Sunderland,T., Bond,J., Walder,K., Augert,G. and Collier,G.
  TITLE     Beacon interacts with cdc2/cdc28-like kinases
  JOURNAL   Biochem Biophys Res Commun 304 (1), 125-129 (2003)
   PUBMED   12705895
REFERENCE   7  (bases 1 to 2504)
  AUTHORS   Katsu,R., Onogi,H., Wada,K., Kawaguchi,Y. and Hagiwara,M.
  TITLE     Novel SR-rich-related protein clasp specifically interacts with
            inactivated Clk4 and induces the exon EB inclusion of Clk
  JOURNAL   J Biol Chem 277 (46), 44220-44228 (2002)
   PUBMED   12169693
REFERENCE   8  (bases 1 to 2504)
  AUTHORS   Schultz,J., Jones,T., Bork,P., Sheer,D., Blencke,S., Steyrer,S.,
            Wellbrock,U., Bevec,D., Ullrich,A. and Wallasch,C.
  TITLE     Molecular characterization of a cDNA encoding functional human CLK4
            kinase and localization to chromosome 5q35 [correction of 4q35]
  JOURNAL   Genomics 71 (3), 368-370 (2001)
   PUBMED   11170754
  REMARK    Erratum:[Genomics 2001 Jun 1;74(2):251]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC015942.1, BC063116.1 and
            CB127989.1.
            
            On Nov 23, 2018 this sequence version replaced NM_020666.2.
            
            Summary: The protein encoded by this gene belongs to the CDC2-like
            protein kinase (CLK) family. This protein kinase can interact with
            and phosphorylate the serine- and arginine-rich (SR) proteins,
            which are known to play an important role in the formation of
            spliceosomes, and thus may be involved in the regulation of
            alternative splicing. Studies in the Israeli sand rat Psammomys
            obesus suggested that the ubiquitin-like 5 (UBL5/BEACON), a highly
            conserved ubiquitin-like protein, may interact with and regulate
            the activity of this kinase. Multiple alternatively spliced
            transcript variants have been observed, but the full-length natures
            of which have not yet been determined. [provided by RefSeq, Jul
            2008].
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.41785.1,
                                           SRR1660803.83941.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000316308.9/ ENSP00000316948.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1544              BC015942.1         4-1547
            1545-1896           BC063116.1         3115-3466
            1897-2504           CB127989.1         19-626
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q35.3"
     gene            1..2504
                     /gene="CLK4"
                     /note="CDC like kinase 4"
                     /db_xref="GeneID:57396"
                     /db_xref="HGNC:HGNC:13659"
                     /db_xref="MIM:607969"
     exon            1..105
                     /gene="CLK4"
                     /inference="alignment:Splign:2.1.0"
     CDS             106..1551
                     /gene="CLK4"
                     /EC_number="2.7.12.1"
                     /note="protein serine threonine kinase Clk4"
                     /codon_start=1
                     /product="dual specificity protein kinase CLK4"
                     /protein_id="NP_065717.1"
                     /db_xref="CCDS:CCDS4437.1"
                     /db_xref="GeneID:57396"
                     /db_xref="HGNC:HGNC:13659"
                     /db_xref="MIM:607969"
                     /translation="MRHSKRTHCPDWDSRESWGHESYRGSHKRKRRSHSSTQENRHCK
                     PHHQFKESDCHYLEARSLNERDYRDRRYVDEYRNDYCEGYVPRHYHRDIESGYRIHCS
                     KSSVRSRRSSPKRKRNRHCSSHQSRSKSHRRKRSRSIEDDEEGHLICQSGDVLRARYE
                     IVDTLGEGAFGKVVECIDHGMDGMHVAVKIVKNVGRYREAARSEIQVLEHLNSTDPNS
                     VFRCVQMLEWFDHHGHVCIVFELLGLSTYDFIKENSFLPFQIDHIRQMAYQICQSINF
                     LHHNKLTHTDLKPENILFVKSDYVVKYNSKMKRDERTLKNTDIKVVDFGSATYDDEHH
                     STLVSTRHYRAPEVILALGWSQPCDVWSIGCILIEYYLGFTVFQTHDSKEHLAMMERI
                     LGPIPQHMIQKTRKRKYFHHNQLDWDEHSSAGRYVRRRCKPLKEFMLCHDEEHEKLFD
                     LVRRMLEYDPTQRITLDEALQHPFFDLLKKK"
     exon            106..266
                     /gene="CLK4"
                     /inference="alignment:Splign:2.1.0"
     exon            267..489
                     /gene="CLK4"
                     /inference="alignment:Splign:2.1.0"
     exon            490..580
                     /gene="CLK4"
                     /inference="alignment:Splign:2.1.0"
     exon            581..647
                     /gene="CLK4"
                     /inference="alignment:Splign:2.1.0"
     exon            648..764
                     /gene="CLK4"
                     /inference="alignment:Splign:2.1.0"
     exon            765..931
                     /gene="CLK4"
                     /inference="alignment:Splign:2.1.0"
     exon            932..1026
                     /gene="CLK4"
                     /inference="alignment:Splign:2.1.0"
     exon            1027..1156
                     /gene="CLK4"
                     /inference="alignment:Splign:2.1.0"
     exon            1157..1239
                     /gene="CLK4"
                     /inference="alignment:Splign:2.1.0"
     exon            1240..1319
                     /gene="CLK4"
                     /inference="alignment:Splign:2.1.0"
     exon            1320..1410
                     /gene="CLK4"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..2504
                     /gene="CLK4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1873..1878
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLK4"
                     /note="hexamer: AGTAAA"
     polyA_site      1898
                     /gene="CLK4"
     regulatory      2486..2491
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLK4"
                     /note="hexamer: CATAAA"
     polyA_site      2504
                     /gene="CLK4"
                     /note="major polyA site"
ORIGIN      
        1 gacggcgctg ccgccatttt gtggggtgtt tgtcgcagcg gccgaggagg gaagacggca
       61 gtttggcgac atttctcggc cgaagggcca tttgcttttg cggagatgcg gcattccaaa
      121 agaactcact gtcctgattg ggatagcaga gaaagctggg gacatgaaag ctatcgtgga
      181 agtcacaagc ggaagaggag atctcatagt agcacacaag agaacaggca ttgtaaacca
      241 catcaccagt ttaaagaatc tgattgtcat tatttagaag caaggtcctt gaatgagcga
      301 gattatcggg accggagata cgttgacgaa tacaggaatg actactgtga aggatatgtt
      361 cctagacatt atcacagaga cattgaaagc gggtatcgaa tccactgcag taaatcttca
      421 gtccgcagca ggagaagcag tcctaaaagg aagcgcaata gacactgttc aagtcatcag
      481 tcacgttcga agagccaccg aaggaaaaga tccaggagta tagaggatga tgaggagggt
      541 cacctgatct gtcaaagtgg agacgttcta agagcaagat atgaaatcgt ggacactttg
      601 ggtgaaggag cctttggcaa agttgtagag tgcattgatc atggcatgga tggcatgcat
      661 gtagcagtga aaatcgtaaa aaatgtaggc cgttaccgtg aagcagctcg ttcagaaatc
      721 caagtattag agcacttaaa tagtactgat cccaatagtg tcttccgatg tgtccagatg
      781 ctagaatggt ttgatcatca tggtcatgtt tgtattgtgt ttgaactact gggacttagt
      841 acttacgatt tcattaaaga aaacagcttt ctgccatttc aaattgacca catcaggcag
      901 atggcgtatc agatctgcca gtcaataaat tttttacatc ataataaatt aacccataca
      961 gatctgaagc ctgaaaatat tttgtttgtg aagtctgact atgtagtcaa atataattct
     1021 aaaatgaaac gtgatgaacg cacactgaaa aacacagata tcaaagttgt tgactttgga
     1081 agtgcaacgt atgatgatga acatcacagt actttggtgt ctacccggca ctacagagct
     1141 cccgaggtca ttttggcttt aggttggtct cagccttgtg atgtttggag cataggttgc
     1201 attcttattg aatattacct tggtttcaca gtctttcaga ctcatgatag taaagagcac
     1261 ctggcaatga tggaacgaat attaggaccc ataccacaac acatgattca gaaaacaaga
     1321 aaacgcaagt attttcacca taaccagcta gattgggatg aacacagttc tgctggtaga
     1381 tatgttagga gacgctgcaa accgttgaag gaatttatgc tttgtcatga tgaagaacat
     1441 gagaaactgt ttgacctggt tcgaagaatg ttagaatatg atccaactca aagaattacc
     1501 ttggatgaag cattgcagca tcctttcttt gacttattaa aaaagaaatg aaatgggaat
     1561 cagtggtctt actatatact tctctagaag agattactta agactgtgtc agtcaactaa
     1621 acattctaat atttttgtaa acattaaatt attttgtaca gttaagtgta aatattgtat
     1681 gttttgtatc aatagcataa ttaacttgtt aagcaagtat ggtcttgata atgcattaga
     1741 aaaattaaaa ttaatttttc tttttgaaat taccattttt aaataccttt gaaatatcct
     1801 ttgtgtccag tgataaatgt gattgatctt gccttttgta catggaggtc acctctgaag
     1861 tgattttttt tgagtaaaag gaaatcttga ctactttata ttcttaaagg aatattcttt
     1921 atatacttca aatttagaac ttaactttaa aagtttttct tctgtaattg ttgaacgggt
     1981 gattattatt aactctagat aagcaggtac tagaaaccaa aactcagaaa atgtttactg
     2041 ttagaattct attaaatttt aagtgttgta ttctttttca ttgggtgatg tcagggtgat
     2101 aaccagacat tcatggaaag gcatgcagtt tgtccattgt gacagtttgt ttaataaaac
     2161 cacatacaca ctttatttaa gattaaaatc taactggaaa gtcagcttgg aaaatggaca
     2221 tttccaagta tgtttggtga gtcacagata taaaaataga aattctgatg agaggtttca
     2281 gtttttaata ccaagtcctt aggagtctta acattggcca gcatctgttt atcaaatgac
     2341 ataaatacgt aaacctataa gaattaagtt tattaattag gcaatttatg tctgtgataa
     2401 ttcttacggg agaaagagga tttgattgga aagcagtttg ggaagaaagt gctgctgaaa
     2461 tttccagaat ttaattgatt ggttacataa actttttgac ttca
//
