LOCUS       NM_001294338            2154 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens CDC like kinase 2 (CLK2), transcript variant 1, mRNA.
ACCESSION   NM_001294338 XM_005244876 XM_005276742
VERSION     NM_001294338.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Zhou,H.B., Yang,L., Liu,S.F., Xu,X.H., Chen,Z., Li,Y.X., Yuan,J.H.
            and Wei,Y.C.
  TITLE     CDC Like Kinase 2 plays an oncogenic role in colorectal cancer via
            modulating the Wnt/beta-catenin signaling
  JOURNAL   Neoplasma 69 (3), 657-669 (2022)
   PUBMED   35293765
  REMARK    GeneRIF: CDC Like Kinase 2 plays an oncogenic role in colorectal
            cancer via modulating the Wnt/beta-catenin signaling.
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 2154)
  AUTHORS   Liu,B., Kong,X., Wang,R. and Xin,C.
  TITLE     CLK2 promotes occurrence and development of non-small cell lung
            cancer
  JOURNAL   J BUON 26 (1), 58-64 (2021)
   PUBMED   33721432
  REMARK    GeneRIF: CLK2 promotes occurrence and development of non-small cell
            lung cancer.
REFERENCE   4  (bases 1 to 2154)
  AUTHORS   Buljan,M., Ciuffa,R., van Drogen,A., Vichalkovski,A., Mehnert,M.,
            Rosenberger,G., Lee,S., Varjosalo,M., Pernas,L.E., Spegg,V.,
            Snijder,B., Aebersold,R. and Gstaiger,M.
  TITLE     Kinase Interaction Network Expands Functional and Disease Roles of
            Human Kinases
  JOURNAL   Mol Cell 79 (3), 504-520 (2020)
   PUBMED   32707033
REFERENCE   5  (bases 1 to 2154)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 2154)
  AUTHORS   Nayler,O., Schnorrer,F., Stamm,S. and Ullrich,A.
  TITLE     The cellular localization of the murine serine/arginine-rich
            protein kinase CLK2 is regulated by serine 141 autophosphorylation
  JOURNAL   J Biol Chem 273 (51), 34341-34348 (1998)
   PUBMED   9852100
REFERENCE   7  (bases 1 to 2154)
  AUTHORS   Duncan,P.I., Stojdl,D.F., Marius,R.M., Scheit,K.H. and Bell,J.C.
  TITLE     The Clk2 and Clk3 dual-specificity protein kinases regulate the
            intranuclear distribution of SR proteins and influence pre-mRNA
            splicing
  JOURNAL   Exp Cell Res 241 (2), 300-308 (1998)
   PUBMED   9637771
REFERENCE   8  (bases 1 to 2154)
  AUTHORS   Winfield,S.L., Tayebi,N., Martin,B.M., Ginns,E.I. and Sidransky,E.
  TITLE     Identification of three additional genes contiguous to the
            glucocerebrosidase locus on chromosome 1q21: implications for
            Gaucher disease
  JOURNAL   Genome Res 7 (10), 1020-1026 (1997)
   PUBMED   9331372
REFERENCE   9  (bases 1 to 2154)
  AUTHORS   Lee,K., Du,C., Horn,M. and Rabinow,L.
  TITLE     Activity and autophosphorylation of LAMMER protein kinases
  JOURNAL   J Biol Chem 271 (44), 27299-27303 (1996)
   PUBMED   8910305
REFERENCE   10 (bases 1 to 2154)
  AUTHORS   Hanes,J., von der Kammer,H., Klaudiny,J. and Scheit,K.H.
  TITLE     Characterization by cDNA cloning of two new human protein kinases.
            Evidence by sequence comparison of a new family of mammalian
            protein kinases
  JOURNAL   J Mol Biol 244 (5), 665-672 (1994)
   PUBMED   7990150
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY049688.1, AL713999.28,
            BC053603.1 and BC014067.2.
            
            On Nov 23, 2018 this sequence version replaced NM_001294338.1.
            
            Summary: This gene encodes a dual specificity protein kinase that
            phosphorylates serine/threonine and tyrosine-containing substrates.
            Activity of this protein regulates serine- and arginine-rich (SR)
            proteins of the spliceosomal complex, thereby influencing
            alternative transcript splicing. Chromosomal translocations have
            been characterized between this locus and the PAFAH1B3
            (platelet-activating factor acetylhydrolase 1b, catalytic subunit 3
            (29kDa)) gene on chromosome 19, resulting in the production of a
            fusion protein. Note that this gene is distinct from the TELO2 gene
            (GeneID:9894), which shares the CLK2 alias, but encodes a protein
            that is involved in telomere length regulation. There is a
            pseudogene for this gene on chromosome 7. Alternative splicing
            results in multiple transcript variants. [provided by RefSeq, Jun
            2014].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.14770.1,
                                           SRR3476690.99313.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000368361.9/ ENSP00000357345.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-55                HY049688.1         28-82
            56-57               AL713999.28        101728-101729       c
            58-1995             BC053603.1         1-1938
            1996-2154           BC014067.2         1776-1934
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q22"
     gene            1..2154
                     /gene="CLK2"
                     /note="CDC like kinase 2"
                     /db_xref="GeneID:1196"
                     /db_xref="HGNC:HGNC:2069"
                     /db_xref="MIM:602989"
     exon            1..304
                     /gene="CLK2"
                     /inference="alignment:Splign:2.1.0"
     CDS             305..1804
                     /gene="CLK2"
                     /EC_number="2.7.12.1"
                     /note="isoform 1 is encoded by transcript variant 1; dual
                     specificity protein kinase CLK2; CLK kinase"
                     /codon_start=1
                     /product="dual specificity protein kinase CLK2 isoform 1"
                     /protein_id="NP_001281267.1"
                     /db_xref="CCDS:CCDS72939.1"
                     /db_xref="GeneID:1196"
                     /db_xref="HGNC:HGNC:2069"
                     /db_xref="MIM:602989"
                     /translation="MPHPRRYHSSERGSRGSYREHYRSRKHKRRRSRSWSSSSDRTRR
                     RRREDSYHVRSRSSYDDRSSDRRVYDRRYCGSYRRNDYSRDRGDAYYDTDYRHSYEYQ
                     RENSSYRSQRSSRRKHRRRRRRSRTFSRSSSQHSSRRAKSVEDDAEGHLIYHVGDWLQ
                     ERYEIVSTLGEGTFGRVVQCVDHRRGGARVALKIIKNVEKYKEAARLEINVLEKINEK
                     DPDNKNLCVQMFDWFDYHGHMCISFELLGLSTFDFLKDNNYLPYPIHQVRHMAFQLCQ
                     AVKFLHDNKLTHTDLKPENILFVNSDYELTYNLEKKRDERSVKSTAVRVVDFGSATFD
                     HEHHSTIVSTRHYRAPEVILELGWSQPCDVWSIGCIIFEYYVGFTLFQTHDNREHLAM
                     MERILGPIPSRMIRKTRKQKYFYRGRLDWDENTSAGRYVRENCKPLRRYLTSEAEEHH
                     QLFDLIESMLEYEPAKRLTLGEALQHPFFARLRAEPPNKLWDSSRDISR"
     exon            305..474
                     /gene="CLK2"
                     /inference="alignment:Splign:2.1.0"
     exon            475..703
                     /gene="CLK2"
                     /inference="alignment:Splign:2.1.0"
     exon            704..791
                     /gene="CLK2"
                     /inference="alignment:Splign:2.1.0"
     exon            792..858
                     /gene="CLK2"
                     /inference="alignment:Splign:2.1.0"
     exon            859..975
                     /gene="CLK2"
                     /inference="alignment:Splign:2.1.0"
     exon            976..1142
                     /gene="CLK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1143..1237
                     /gene="CLK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1238..1367
                     /gene="CLK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1368..1450
                     /gene="CLK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1451..1530
                     /gene="CLK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1531..1621
                     /gene="CLK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1622..2154
                     /gene="CLK2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2125..2130
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLK2"
                     /note="hexamer: AATAAA"
     polyA_site      2154
                     /gene="CLK2"
                     /note="major polyA site"
ORIGIN      
        1 gtgcgtacgt gctcgctcac ggcggcggcg gcggagcgga gaggccagag ccggagaccg
       61 agctgggatc gggccccggg cgggggcggt gcgagcggcg ccaagcagat cttaggggcg
      121 gggacggagc cggggcgggc gggactgaag cggagcccgg gaacggggcg ggaggtccca
      181 gggtcccggg ttgggggggt ggagcagcat ttcgtcgccg cgggggtgcc gggactccgg
      241 ccgcagtgtc gccgccatca cggacttcct gtgggacaag cgcacgggcc tcgccgccag
      301 aacgatgccg catcctcgaa ggtaccactc ctcagagcga ggcagccggg ggagttaccg
      361 tgaacactat cggagccgaa agcataagcg acgaagaagt cgctcctggt caagtagtag
      421 tgaccggaca cgacggcgtc ggcgagagga cagctaccat gtccgttctc gaagcagtta
      481 tgatgatcgt tcgtccgacc ggagggtgta tgaccggcga tactgtggca gctacagacg
      541 caacgattat agccgggatc ggggagatgc ctactatgac acagactatc ggcattccta
      601 tgaatatcag cgggagaaca gcagttaccg cagccagcgc agcagccgga ggaagcacag
      661 acggcggagg aggcgcagcc ggacatttag ccgctcatct tcgcagcaca gcagccggag
      721 agccaagagt gtagaggacg acgctgaggg ccacctcatc taccacgtcg gggactggct
      781 acaagagcga tatgaaatcg ttagcacctt aggagagggg accttcggcc gagttgtaca
      841 atgtgttgac catcgcaggg gtggggctcg agttgccctg aagatcatta agaatgtgga
      901 gaagtacaag gaagcagctc gacttgagat caacgtgcta gagaaaatca atgagaaaga
      961 ccctgacaac aagaacctct gtgtccagat gtttgactgg tttgactacc atggccacat
     1021 gtgtatctcc tttgagcttc tgggccttag caccttcgat ttcctcaaag acaacaacta
     1081 cctgccctac cccatccacc aagtgcgcca catggccttc cagctgtgcc aggctgtcaa
     1141 gttcctccat gataacaagc tgacacatac agacctcaag cctgaaaata ttctgtttgt
     1201 gaattcagac tatgagctca cctacaacct agagaagaag cgagatgagc gcagtgtgaa
     1261 gagcacagct gtgcgggtgg tagactttgg cagtgccacc tttgaccatg agcaccatag
     1321 caccattgtc tccactcgcc attaccgagc accagaagtc atccttgagt tgggctggtc
     1381 acagccttgt gatgtgtgga gtataggctg catcatcttt gaatactatg tgggattcac
     1441 cctcttccag acccatgaca acagagagca tctagccatg atggaaagga tcttgggtcc
     1501 tatcccttcc cggatgatcc gaaagacaag aaagcagaaa tatttttacc ggggtcgcct
     1561 ggattgggat gagaacacat cagctgggcg ctatgttcgt gagaactgca aaccgctgcg
     1621 gcggtatctg acctcagagg cagaggaaca ccaccagctc ttcgatctga ttgaaagcat
     1681 gctagagtat gaaccagcta agcggctgac cttgggtgaa gcccttcagc atcctttctt
     1741 cgcccgcctt cgggctgagc cgcccaacaa gttgtgggac tccagtcggg atatcagtcg
     1801 gtgacgatca ggccctgggc ccccctgcat cttttatagc agtgggtgtc cagtccagga
     1861 cactggtgct tttttataca agagaacgag ccagagttca ctccttcctc ctggctctct
     1921 atatacctgt gaatatgtga aatagtgtaa atatgaaaga acttgtacct atcacttcaa
     1981 cccctgcctt gtacataata ctattccatc cacacagttt ccaccctcac ctgccccctc
     2041 atacggagtt ggatgggggc cgagtgaggt aaccaggtgg catctacccc atgttttata
     2101 aggaattttg tacagtcttt gtgaaataaa ataacgtgct tcatttgacc ccca
//
