LOCUS       NM_004362               2725 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens calmegin (CLGN), transcript variant 1, mRNA.
ACCESSION   NM_004362
VERSION     NM_004362.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Li,C., He,J., Chen,J., Zhao,J., Gu,D., Hixson,J.E., Rao,D.C.,
            Jaquish,C.E., Gu,C.C., Chen,J., Huang,J., Chen,S. and Kelly,T.N.
  TITLE     Genome-Wide Gene-Sodium Interaction Analyses on Blood Pressure: The
            Genetic Epidemiology Network of Salt-Sensitivity Study
  JOURNAL   Hypertension 68 (2), 348-355 (2016)
   PUBMED   27271309
  REMARK    GeneRIF: In gene-based analysis CLGN1 represented one of a cluster
            of genes, which interacted with sodium to influence Blood Pressure.
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Boldt,K., van Reeuwijk,J., Lu,Q., Koutroumpas,K., Nguyen,T.M.,
            Texier,Y., van Beersum,S.E., Horn,N., Willer,J.R., Mans,D.A.,
            Dougherty,G., Lamers,I.J., Coene,K.L., Arts,H.H., Betts,M.J.,
            Beyer,T., Bolat,E., Gloeckner,C.J., Haidari,K., Hetterschijt,L.,
            Iaconis,D., Jenkins,D., Klose,F., Knapp,B., Latour,B.,
            Letteboer,S.J., Marcelis,C.L., Mitic,D., Morleo,M., Oud,M.M.,
            Riemersma,M., Rix,S., Terhal,P.A., Toedt,G., van Dam,T.J., de
            Vrieze,E., Wissinger,Y., Wu,K.M., Apic,G., Beales,P.L.,
            Blacque,O.E., Gibson,T.J., Huynen,M.A., Katsanis,N., Kremer,H.,
            Omran,H., van Wijk,E., Wolfrum,U., Kepes,F., Davis,E.E., Franco,B.,
            Giles,R.H., Ueffing,M., Russell,R.B. and Roepman,R.
  CONSRTM   UK10K Rare Diseases Group
  TITLE     An organelle-specific protein landscape identifies novel diseases
            and molecular mechanisms
  JOURNAL   Nat Commun 7, 11491 (2016)
   PUBMED   27173435
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2725)
  AUTHORS   Gupta,G.D., Coyaud,E., Goncalves,J., Mojarad,B.A., Liu,Y., Wu,Q.,
            Gheiratmand,L., Comartin,D., Tkach,J.M., Cheung,S.W., Bashkurov,M.,
            Hasegan,M., Knight,J.D., Lin,Z.Y., Schueler,M., Hildebrandt,F.,
            Moffat,J., Gingras,A.C., Raught,B. and Pelletier,L.
  TITLE     A Dynamic Protein Interaction Landscape of the Human
            Centrosome-Cilium Interface
  JOURNAL   Cell 163 (6), 1484-1499 (2015)
   PUBMED   26638075
REFERENCE   4  (bases 1 to 2725)
  AUTHORS   Sakono,M., Seko,A., Takeda,Y., Aikawa,J., Hachisu,M., Koizumi,A.,
            Fujikawa,K. and Ito,Y.
  TITLE     Glycan specificity of a testis-specific lectin chaperone calmegin
            and effects of hydrophobic interactions
  JOURNAL   Biochim Biophys Acta 1840 (9), 2904-2913 (2014)
   PUBMED   24769397
  REMARK    GeneRIF: Although their similarity in carbohydrate binding
            specificities is high, there seems to be some differences in the
            mode of substrate recognition between calmegin and calnexin.
REFERENCE   5  (bases 1 to 2725)
  AUTHORS   Benjamin,E.J., Dupuis,J., Larson,M.G., Lunetta,K.L., Booth,S.L.,
            Govindaraju,D.R., Kathiresan,S., Keaney,J.F. Jr., Keyes,M.J.,
            Lin,J.P., Meigs,J.B., Robins,S.J., Rong,J., Schnabel,R., Vita,J.A.,
            Wang,T.J., Wilson,P.W., Wolf,P.A. and Vasan,R.S.
  TITLE     Genome-wide association with select biomarker traits in the
            Framingham Heart Study
  JOURNAL   BMC Med Genet 8 Suppl 1 (Suppl 1), S11 (2007)
   PUBMED   17903293
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2725)
  AUTHORS   Ikawa,M., Nakanishi,T., Yamada,S., Wada,I., Kominami,K., Tanaka,H.,
            Nozaki,M., Nishimune,Y. and Okabe,M.
  TITLE     Calmegin is required for fertilin alpha/beta heterodimerization and
            sperm fertility
  JOURNAL   Dev Biol 240 (1), 254-261 (2001)
   PUBMED   11784061
REFERENCE   7  (bases 1 to 2725)
  AUTHORS   Yoshinaga,K., Tanii,I. and Toshimori,K.
  TITLE     Molecular chaperone calmegin localization to the endoplasmic
            reticulum of meiotic and post-meiotic germ cells in the mouse
            testis
  JOURNAL   Arch Histol Cytol 62 (3), 283-293 (1999)
   PUBMED   10495883
REFERENCE   8  (bases 1 to 2725)
  AUTHORS   Tanaka,H., Ikawa,M., Tsuchida,J., Nozaki,M., Suzuki,M.,
            Fujiwara,T., Okabe,M. and Nishimune,Y.
  TITLE     Cloning and characterization of the human Calmegin gene encoding
            putative testis-specific chaperone
  JOURNAL   Gene 204 (1-2), 159-163 (1997)
   PUBMED   9434179
REFERENCE   9  (bases 1 to 2725)
  AUTHORS   Ikawa,M., Wada,I., Kominami,K., Watanabe,D., Toshimori,K.,
            Nishimune,Y. and Okabe,M.
  TITLE     The putative chaperone calmegin is required for sperm fertility
  JOURNAL   Nature 387 (6633), 607-611 (1997)
   PUBMED   9177349
REFERENCE   10 (bases 1 to 2725)
  AUTHORS   Watanabe,D., Yamada,K., Nishina,Y., Tajima,Y., Koshimizu,U.,
            Nagata,A. and Nishimune,Y.
  TITLE     Molecular cloning of a novel Ca(2+)-binding protein (calmegin)
            specifically expressed during male meiotic germ cell development
  JOURNAL   J Biol Chem 269 (10), 7744-7749 (1994)
   PUBMED   8126001
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC396076.1, BC028357.2 and
            D86322.1.
            
            On Nov 22, 2018 this sequence version replaced NM_004362.2.
            
            Summary: Calmegin is a testis-specific endoplasmic reticulum
            chaperone protein.  CLGN may play a role in spermatogeneisis and
            infertility. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) is the shorter transcript.
            Both variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D86322.1, SRR1803617.222683.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000325617.10/ ENSP00000326699.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-177               DC396076.1         15-191
            178-2410            BC028357.2         145-2377
            2411-2725           D86322.1           2390-2704
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q31.1"
     gene            1..2725
                     /gene="CLGN"
                     /note="calmegin"
                     /db_xref="GeneID:1047"
                     /db_xref="HGNC:HGNC:2060"
                     /db_xref="MIM:601858"
     exon            1..112
                     /gene="CLGN"
                     /inference="alignment:Splign:2.1.0"
     exon            113..265
                     /gene="CLGN"
                     /inference="alignment:Splign:2.1.0"
     CDS             122..1954
                     /gene="CLGN"
                     /note="testis tissue sperm-binding protein Li 79P"
                     /codon_start=1
                     /product="calmegin precursor"
                     /protein_id="NP_004353.1"
                     /db_xref="CCDS:CCDS3751.1"
                     /db_xref="GeneID:1047"
                     /db_xref="HGNC:HGNC:2060"
                     /db_xref="MIM:601858"
                     /translation="MHFQAFWLCLGLLFISINAEFMDDDVETEDFEENSEEIDVNESE
                     LSSEIKYKTPQPIGEVYFAETFDSGRLAGWVLSKAKKDDMDEEISIYDGRWEIEELKE
                     NQVPGDRGLVLKSRAKHHAISAVLAKPFIFADKPLIVQYEVNFQDGIDCGGAYIKLLA
                     DTDDLILENFYDKTSYIIMFGPDKCGEDYKLHFIFRHKHPKTGVFEEKHAKPPDVDLK
                     KFFTDRKTHLYTLVMNPDDTFEVLVDQTVVNKGSLLEDVVPPIKPPKEIEDPNDKKPE
                     EWDERAKIPDPSAVKPEDWDESEPAQIEDSSVVKPAGWLDDEPKFIPDPNAEKPDDWN
                     EDTDGEWEAPQILNPACRIGCGEWKPPMIDNPKYKGVWRPPLVDNPNYQGIWSPRKIP
                     NPDYFEDDHPFLLTSFSALGLELWSMTSDIYFDNFIICSEKEVADHWAADGWRWKIMI
                     ANANKPGVLKQLMAAAEGHPWLWLIYLVTAGVPIALITSFCWPRKVKKKHKDTEYKKT
                     DICIPQTKGVLEQEEKEEKAALEKPMDLEEEKKQNDGEMLEKEEESEPEEKSEEEIEI
                     IEGQEESNQSNKSGSEDEMKEADESTGSGDGPIKSVRKRRVRKD"
     exon            266..339
                     /gene="CLGN"
                     /inference="alignment:Splign:2.1.0"
     exon            340..398
                     /gene="CLGN"
                     /inference="alignment:Splign:2.1.0"
     exon            399..540
                     /gene="CLGN"
                     /inference="alignment:Splign:2.1.0"
     exon            541..622
                     /gene="CLGN"
                     /inference="alignment:Splign:2.1.0"
     exon            623..815
                     /gene="CLGN"
                     /inference="alignment:Splign:2.1.0"
     exon            816..1005
                     /gene="CLGN"
                     /inference="alignment:Splign:2.1.0"
     exon            1006..1119
                     /gene="CLGN"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1270
                     /gene="CLGN"
                     /inference="alignment:Splign:2.1.0"
     exon            1271..1486
                     /gene="CLGN"
                     /inference="alignment:Splign:2.1.0"
     exon            1487..1612
                     /gene="CLGN"
                     /inference="alignment:Splign:2.1.0"
     exon            1613..1772
                     /gene="CLGN"
                     /inference="alignment:Splign:2.1.0"
     exon            1773..1873
                     /gene="CLGN"
                     /inference="alignment:Splign:2.1.0"
     exon            1874..2725
                     /gene="CLGN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2385..2390
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLGN"
                     /note="hexamer: AATAAA"
     polyA_site      2422
                     /gene="CLGN"
                     /note="major polyA site"
     regulatory      2696..2701
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLGN"
                     /note="hexamer: AATAAA"
     polyA_site      2725
                     /gene="CLGN"
ORIGIN      
        1 agtagtcgct gctgcgcggc cgccggcggg actggtctga agagacgcgg ggaccaagtg
       61 gcaacgactt ggacatctga gctgtcactg ccgaaaacag gccgcaagag agataatcaa
      121 tatgcatttc caagcctttt ggctatgttt gggtcttctg ttcatctcaa ttaatgcaga
      181 atttatggat gatgatgttg agacggaaga ctttgaagaa aattcagaag aaattgatgt
      241 taatgaaagt gaactttcct cagagattaa atataagaca cctcaaccta taggagaagt
      301 atattttgca gaaacttttg atagtggaag gttggctgga tgggtcttat caaaagcaaa
      361 gaaagatgac atggatgagg aaatttcaat atacgatgga agatgggaaa ttgaagagtt
      421 gaaagaaaac caggtacctg gtgacagagg actggtatta aaatctagag caaagcatca
      481 tgcaatatct gctgtattag caaaaccatt catttttgct gataaaccct tgatagttca
      541 atatgaagta aattttcaag atggtattga ttgtggaggt gcatacatta aactcctagc
      601 agacactgat gatttgattc tggaaaactt ttatgataaa acatcctata tcattatgtt
      661 tggaccagat aaatgtggag aagattataa acttcatttt atcttcagac ataaacatcc
      721 caaaactgga gttttcgaag agaaacatgc caaacctcca gatgtagacc ttaaaaagtt
      781 ctttacagac aggaagactc atctttatac ccttgtgatg aatccagatg acacatttga
      841 ggtgttagtt gatcaaacag ttgtaaacaa aggaagcctc ctagaggatg tggttcctcc
      901 tatcaaacct cccaaagaaa ttgaagatcc caatgataaa aaacctgagg aatgggatga
      961 aagagcaaaa attcctgatc cttctgccgt caaaccagaa gactgggatg aaagtgaacc
     1021 tgcccaaata gaagattcaa gtgttgttaa acctgctggc tggcttgatg atgaaccaaa
     1081 atttatccct gatcctaatg ctgaaaaacc tgatgactgg aatgaagaca cggatggaga
     1141 atgggaggca cctcagattc ttaatccagc atgtcggatt gggtgtggtg agtggaaacc
     1201 tcccatgata gataacccaa aatacaaagg agtatggaga cctccactgg tcgataatcc
     1261 taactatcag ggaatctgga gtcctcgaaa aattcctaat ccagattatt tcgaagatga
     1321 tcatccattt cttctgactt ctttcagtgc tcttggttta gagctttggt ctatgacctc
     1381 tgatatctac tttgataatt ttattatctg ttcggaaaag gaagtagcag atcactgggc
     1441 tgcagatggt tggagatgga aaataatgat agcaaatgct aataagcctg gtgtattaaa
     1501 acagttaatg gcagctgctg aagggcaccc atggctttgg ttgatttatc ttgtgacagc
     1561 aggagtgcca atagcattaa ttacttcatt ttgttggcca agaaaagtaa agaaaaaaca
     1621 taaagataca gagtataaaa aaaccgacat atgtatacca caaacaaaag gagtactaga
     1681 gcaagaagaa aaggaagaga aagcagccct ggaaaaacca atggacctgg aagaggaaaa
     1741 aaagcaaaat gatggtgaaa tgcttgaaaa agaagaggaa agtgaacctg aggaaaagag
     1801 tgaagaagaa attgaaatca tagaagggca agaagaaagt aatcaatcaa ataagtctgg
     1861 gtcagaggat gagatgaaag aagcagatga gagcacagga tctggagatg ggccgataaa
     1921 gtcagtacgc aaaagaagag tacgaaagga ctaaactaga ttgaaatatt tttaattccc
     1981 gagagggatg tttggcattg taaaaatcag catgccagac ctgaacttta atcagtctgc
     2041 acatcctgtt tctaatatct agcaacatta tattctttca gacatttatt ttagtccttc
     2101 atttcagagg aaaaagaagc aactttgaag ttacctcatc tttgaattta gaataaaagt
     2161 ggcacattac atatcggatc taagagatta ataccattag aagttacaca gttttagttg
     2221 tttggagata gttttggttt gtacagaaca aaataatatg tagcagcttc attgctattg
     2281 gaaaaatcag ttattggaat ttccacttaa atggctatac aacaatataa ctggtagttc
     2341 tataataaaa atgagcatat gttctgttgt gaagagctaa atgcaataaa gtttctgtat
     2401 ggttgtttga ttctatcaac aattgaaagt gttgtatatg acccacattt acctagtttg
     2461 tgtcaaatta tagttacagt gagttgtttg cttaaattat agattccttt aaggacatgc
     2521 cttgttcata aaatcactgg attatattgc agcatatttt acatttgaat acaaggataa
     2581 tgggttttat caaaacaaaa tgatgtacag attttttttc aagtttttat agttgcttta
     2641 tgccagagtg gtttacccca ttcacaaaat ttcttatgca tacattgcta ttgaaaataa
     2701 aatttaaata ttttttcatc ctgaa
//
